100% of the eggs laid by Ras85D05703/Ras85De1B mothers have a single central dorsal appendage. This single appendage has a lumen, and the cell rearrangements occuring duing its development resemble those occuring during the morphogenesis of each of wild-type appendage despite some of the cells involved being mis-shapen.
Ras85De1B suppresses the increased size of Apc2g10ApcQ8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed. This reduction in cell number is due to reduced proliferation, rather than increased cell death: TUNEL expression is unchanged and expression of the apoptotic protein BacA\p35 has no effect on the phenotype. The remaining increase in clone size seen in the Ras85De1B, Apc2g10, ApcQ8 triple mutant flies is fully suppressed upon expression of panΔN.Scer\UAS under the control of Scer\GAL4Act.PU, and no increase in apoptosis is seen in these flies.
The cell-autonomous overgrowth phenotype found in Uba1B2 homozygous and Uba1B1/Uba1B2 trans-heterozygous eye discs is suppressed in a Ras85De1B heterozygous background.
A Ras85De1B/+ background dramatically suppresses the lethality found in Uba1B1/Uba1B2 homozygotes.
Uba1B1/Uba1B2 eyes in a Ras85De1B heterozygous background show decreased suppression of egrScer\UAS.cMa (under the control of Scer\GAL4GMR.PF) induced cell death.
Overall cell proliferation in Uba1B1/Uba1B2 eye discs is reduced in a Ras85De1B/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe.
A Ras85De1B/+ background dramatically suppresses the non-autonomous overgrowth phenotype found in Uba1A1 mosaic eye clones.
Ras85De1B/+, S05671/+ double mutants have a stronger missing-bract phenotype than either mutant alone. (2% of bracts in the tibia and 60% in the basitarsus of the second leg remain). The addition of Ras85Dhs.M11.2 under heat shock control partially suppresses this phenotype.
Ras85De1B/+ significantly restores the organisation of cysts and membrane deposition in the testes of homozygous Df(3R)rn20 males. These animals show reduced eclosion and have a short life span.
Ras85De1B/+ in a Df(3R)rn20 homozygous background results in a significant increase in the number of pigment cells and closer packing than seen in a Df(3R)rn20 single mutant background. The ommatidia remain strangely shaped and have variable numbers of outer and inner photoreceptor cells. There is a drastic reduction in the depth of the retina.
pbhs.PBRas85De1B double heterozygotes have a partially penetrant truncation of wing veins L4 and L5, and the frequency of ectopic bristle formation on the distal end of wing vein L3 (seen in pbhs.PB heterozygotes) is increased to 90% for females and 50% for males. The enhancement of the pbhs.PB antenna to maxillary transformation phenotype caused by Gap1sxt-BJ61 is reduced if the flies also carry one copy of Ras85De1B. The enhancement of the Gap1sxt-BJ61 ectopic wing vein phenotype caused by pbhs.PB is reduced if the flies also carry one copy of Ras85De1B. The enhancement of the Df(3R)Ubx109/+ phenotype by Gap1sxt-BJ61 is partially reversed if the flies also carry Ras85De1B.