FlyBase Allele Report: Dmel\Ras85D[e1B]

General Information

Symbol

Dmel\Ras85D^e1B

Species

D. melanogaster

Name

FlyBase ID

FBal0030101

Feature type

allele

Associated gene

Dmel\Ras85D

Associated Insertion(s)

Carried in Construct

Also Known As

Ras1^e1B, ras^e1B, Ras85D^E62K, E(sev)3C^elb, E(sev)3C^e1B

Key Links

Genomic Maps

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Allele class

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

Mutagen

ethyl methanesulfonate

(Schnorr and Berg, 1996)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

point_mutation

3R:9,511,952..9,511,952 [-]

Nucleotide change:

G9511952A

Amino acid change:

E62K | Ras85D-PA

Reported amino acid change:

E62K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Simon et al., 1991)

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

Cytology

Nature of the lesion

Statement

Reference

Missense mutation.

(Boube et al., 1997)

Mutation at residue 62 in the GTP-binding domain.

(Kussick et al., 1993)

Amino acid replacement: E62K.

(Simon et al., 1991)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates alveolar rhabdomyosarcoma

modeled by Hsap\FOXO1::Hsap\PAX7^UAS.cGa

(Galindo et al., 2006)

exacerbates viral infectious disease

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates infectious mononucleosis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

exacerbates Epstein-Barr virus hepatitis

modeled by EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

ameliorates gastrointestinal system cancer

modeled by Apc2^g10, Apc^Q8

(Wang et al., 2013)

exacerbates tuberous sclerosis

modeled by Tsc1^UAS.cTa, gig^UAS.cTa

(Tapon et al., 2001)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

lethal

(Schnorr and Berg, 1996)

lethal | recessive

(Simon et al., 1991)

partially lethal - majority die

(Schnorr and Berg, 1996)

visible

(Schnorr and Berg, 1996)

Phenotype Manifest In

bract

(Held, 2002)

dorsal appendage

(Schnorr and Berg, 1996)

dorsal appendage | maternal effect (with Ras85D⁰⁵⁷⁰³)

(Ward and Berg, 2005)

mesothoracic tarsal segment 1 & bract

(Held, 2002)

ommatidium

(Schnorr and Berg, 1996)

Detailed Description

Statement

Reference

Heterozygous Ras85D^e1B flies exhibit normal life spans.

(Tong et al., 2007)

Ras85D^e1B/+ flies show a wild-type number of rhabdomeres in their eyes.

(Kim et al., 2006)

100% of the eggs laid by Ras85D⁰⁵⁷⁰³/Ras85D^e1B mothers have a single central dorsal appendage. This single appendage has a lumen, and the cell rearrangements occuring duing its development resemble those occuring during the morphogenesis of each of wild-type appendage despite some of the cells involved being mis-shapen.

(Ward and Berg, 2005)

Somatic clones of Ras85D^e1B in the wing disc peripodial membrane have reduced viability compared to wild-type twin spot cells.

(Pallavi and Shashidhara, 2003)

Heterozygotes have almost wild-type bracts in the tibia (100%) and in the basitarsus (96%) of the second leg.

(Held, 2002)

Heterozygous loss of Ras85D has no effect on size of Nf1 mutant pupae.

(The et al., 1997)

No effect on the faf eye phenotype.

(Huang and Fischer-Vize, 1996)

Ras85D^ΔC17b/Ras85D^e1B and Df(3R)ΔC40b/Ras85D^e1B transheterozygotes are lethal. Ras85D^e1B/Ras85D⁰⁵⁷⁰³ transheterozygotes are semi-lethal. Females transheterozygotes with Ras85D⁰⁵⁷⁰³ lay eggs with a single dorsal appendage, 84% of eggs hatch and develop to normal larvae. Transheterozygotes with Ras85D⁰⁵⁷⁰³ exhibit extremely rough eyes (many fused and misplaced ommatidia).

(Schnorr and Berg, 1996)

Reduces the ability of a cell to develop as an R7 cell or as any other photoreceptor.

(Simon et al., 1991)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

Apc2^g10, Apc^Q8, Ras85D^e1B has hyperplasia | somatic clone phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^Act.PU

(Wang et al., 2013)

Ras85D^e1B/Ras85D[+], Uba1^B1/Uba1^B2 has viable phenotype, suppressible by egr^UAS.cMa/Scer\GAL4^GMR.PF

(Yan et al., 2009)

Enhancer of

Statement

Reference

Ras85D^e1B/Ras85D[+] is an enhancer of visible phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Kim et al., 2006)

Ras85D^e1B/Ras85D[+] is an enhancer of visible phenotype of CycE^JP

(Brumby et al., 2004)

Ras85D^e1B is an enhancer of visible | adult stage phenotype of Mkp3^UAS.cKa, Scer\GAL4^sev.PU

(Kim et al., 2004)

Ras85D^e1B is an enhancer of decreased cell number | adult stage phenotype of Mkp3^UAS.cKa, Scer\GAL4^sev.PU

(Kim et al., 2004)

Ras85D^e1B/Ras85D[+] is an enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e1B is an enhancer of abnormal size | adult stage phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa/gig^Scer\UAS.cTa

(Tapon et al., 2001)

Ras85D^e1B is an enhancer of visible | adult stage phenotype of Scer\GAL4^ey.PH, Tsc1^UAS.cTa, gig^UAS.cTa/gig^Scer\UAS.cTa

(Tapon et al., 2001)

Ras85D^e1B is an enhancer of visible phenotype of BacA\p35^GMR.PH, Dp^GMR.PD, E2f1^GMR.PD

(Staehling-Hampton et al., 1999)

Ras85D^e1B is an enhancer of visible phenotype of rux³

(Thomas et al., 1994)

NOT Enhancer of

Statement

Reference

Ras85D^e1B is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Suppressor of

Statement

Reference

Ras85D^e1B/Ras85D[+] is a suppressor | partially of short lived phenotype of Uba1^B1

(Liu and Pfleger, 2013)

Ras85D^e1B is a suppressor of hyperplasia | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Ras85D^e1B is a suppressor of visible phenotype of AdamTS-B^KK108644, Scer\GAL4^Act5C.PU

(Butchar et al., 2012)

Ras85D^e1B/Ras85D[+] is a suppressor | partially of visible phenotype of Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU is a suppressor of visible phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU is a suppressor | partially of increased body size phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+], Scer\GAL4^C-765 is a suppressor of visible phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^C-765

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+] is a suppressor of lethal phenotype of Uba1^B1/Uba1^B2

(Yan et al., 2009)

Ras85D^e1B/Ras85D[+] is a suppressor of increased cell number | somatic clone | cell non-autonomous phenotype of Uba1^A1

(Yan et al., 2009)

Ras85D^e1B/Ras85D[+] is a suppressor of lethal phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e1B/Ras85D[+] is a suppressor of visible phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Ras85D^e1B/Ras85D[+] is a suppressor of visible phenotype of Scer\GAL4^en-e16E, Socs44A^UAS.cRa

(Rawlings et al., 2004)

Ras85D^e1B is a suppressor | partially of abnormal locomotor rhythm phenotype of Nf1^P2

(Williams et al., 2001)

Ras85D^e1B is a suppressor | partially of abnormal locomotor rhythm phenotype of Nf1^P1

(Williams et al., 2001)

Ras85D^e1B is a suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

Ras85D^e1B is a suppressor of visible phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

Ras85D^e1B is a suppressor of lethal phenotype of Ras85D^V12.sev, sev^S11.Tag:MYC

(Maixner et al., 1998)

Ras85D^e1B/Ras85D[+] is a suppressor of visible phenotype of RasGAP1^sxt-BJ61, pb^hs.PB/pb[+]

(Boube et al., 1997)

Ras85D^e1B/Ras85D[+] is a suppressor of visible phenotype of RasGAP1^sxt-BJ61, pb^hs.PB

(Boube et al., 1997)

Ras85D^e1B is a suppressor of visible phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

Ras85D^e1B is a non-suppressor of visible phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Lee et al., 2002)

Ras85D^e1B is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Other

Statement

Reference

Bap170^C1.UAS, Ras85D^e1B, Scer\GAL4^hs.2sev has visible phenotype

(Rendina et al., 2010)

Df(3R)rn20, Ras85D^e1B has partially lethal - majority die | dominant phenotype

(Bergeret et al., 2001)

Df(3R)rn20, Ras85D^e1B has short lived | dominant phenotype

(Bergeret et al., 2001)

Ras85D^V12.sev, Ras85D^e1B, sev^S11.Tag:MYC has viable phenotype

(Maixner et al., 1998)

RasGAP1^sxt-BJ61, Ras85D^e1B, pb^hs.PB/pb[+] has visible phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has visible phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has visible | dominant phenotype

(Boube et al., 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

Ras85D^e1B has mesothoracic tarsal segment 1 & bract phenotype, enhanceable by S⁰⁵⁶⁷¹

(Held, 2002)

Ras85D^e1B has bract phenotype, enhanceable by S⁰⁵⁶⁷¹

(Held, 2002)

Suppressed by

Statement

Reference

Apc2^g10, Apc^Q8, Ras85D^e1B has adult midgut | somatic clone phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^Act.PU

(Wang et al., 2013)

Apc2^g10, Apc^Q8, Ras85D^e1B has intestinal stem cell | somatic clone phenotype, suppressible by pan^ΔN.UAS/Scer\GAL4^Act.PU

(Wang et al., 2013)

Enhancer of

Statement

Reference

Ras85D^e1B is an enhancer of eye phenotype of EBV\BZLF1^GMR.PA

(Adamson and Lajeunesse, 2012)

Ras85D^e1B/Ras85D[+] is an enhancer of ommatidium phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Kim et al., 2006)

Ras85D^e1B/Ras85D[+] is an enhancer of interommatidial bristle phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Kim et al., 2006)

Ras85D^e1B/Ras85D[+] is an enhancer of rhabdomere phenotype of S6kII^UAS.Tag:MYC, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Kim et al., 2006)

Ras85D^e1B/Ras85D[+] is an enhancer of eye photoreceptor cell phenotype of S6kII^{K231R.UAS.Tag:MYC}, Scer\GAL4^hs.2sev

(Kim et al., 2006)

Ras85D^e1B/Ras85D[+] is an enhancer of eye phenotype of CycE^JP

(Brumby et al., 2004)

Ras85D^e1B is an enhancer of eye phenotype of Mkp3^UAS.cKa, Scer\GAL4^sev.PU

(Kim et al., 2004)

Ras85D^e1B is an enhancer of eye photoreceptor cell | adult stage | decreased number phenotype of Mkp3^UAS.cKa, Scer\GAL4^sev.PU

(Kim et al., 2004)

Ras85D^e1B is an enhancer of mesothoracic tarsal segment 1 & bract phenotype of S⁰⁵⁶⁷¹

(Held, 2002)

Ras85D^e1B is an enhancer of mesothoracic tibial bract phenotype of S⁰⁵⁶⁷¹

(Held, 2002)

Ras85D^e1B/Ras85D[+] is an enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e1B is an enhancer of eye phenotype of rux³

(Thomas et al., 1994)

NOT Enhancer of

Statement

Reference

Ras85D^e1B is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e1B is a non-enhancer of phenotype of sina^GMR.PN

(Neufeld et al., 1998)

Suppressor of

Statement

Reference

Ras85D^e1B is a suppressor of adult midgut | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Ras85D^e1B is a suppressor of intestinal stem cell | somatic clone phenotype of Apc2^g10, Apc^Q8

(Wang et al., 2013)

Ras85D^e1B is a suppressor of wing vein | ectopic phenotype of AdamTS-B^KK108644, Scer\GAL4^Act5C.PU

(Butchar et al., 2012)

Ras85D^e1B/Ras85D[+] is a suppressor of posterior crossvein phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein L2 phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+] is a suppressor of posterior crossvein phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^C-765

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein L2 phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^C-765

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+] is a suppressor | partially of eye phenotype of Dcr-2^UAS.cDa, Rabex-5^VDRC.cUa, Scer\GAL4^ey.PU

(Yan et al., 2010)

Ras85D^e1B/Ras85D[+], Scer\GAL4^Act.PU is a suppressor | partially of wing phenotype of Rabex-5^VDRC.cUa, Scer\GAL4^Act.PU

(Yan et al., 2010)

Ras85D^e1B, Scer\GAL4^Tub.PU is a suppressor of wing vein phenotype of Scer\GAL4^Tub.PU, csw^Y279C.UASp

(Oishi et al., 2009)

Ras85D^e1B/Ras85D[+] is a suppressor of eye disc posterior to the morphogenetic furrow phenotype of Uba1^B1/Uba1^B2

(Yan et al., 2009)

Ras85D^e1B/Ras85D[+] is a suppressor of eye disc | somatic clone | cell non-autonomous phenotype of Uba1^A1

(Yan et al., 2009)

Ras85D^e1B/Ras85D[+] is a suppressor of embryonic/larval somatic muscle cell phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e1B/Ras85D[+] is a suppressor of embryonic/larval muscle system phenotype of Hsap\FOXO1::Hsap\PAX7^UAS.cGa, Scer\GAL4^Mhc.PW

(Galindo et al., 2006)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^Tub.PU, csw^N308D.UASp

(Oishi et al., 2006)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4^en-e16E, Socs44A^UAS.cRa

(Rawlings et al., 2004)

Ras85D^e1B is a suppressor | partially of ommatidium phenotype of Krn^UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Reich and Shilo, 2002)

Ras85D^e1B is a suppressor | partially of eye phenotype of Krn^UAS.cRa, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Reich and Shilo, 2002)

Ras85D^e1B is a suppressor of eye phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Ishimaru et al., 1999)

Ras85D^e1B is a suppressor of wing vein L3 phenotype of C3G^{UAS.Tag:MYC,Tag:M(c-SRC)}, Scer\GAL4^dpp.blk1

(Ishimaru et al., 1999)

Ras85D^e1B/Ras85D[+] is a suppressor of phenotype of Src42A^Su(Raf)1-1

(Zhang et al., 1999)

Ras85D^e1B/Ras85D[+] is a suppressor of antenna phenotype of RasGAP1^sxt-BJ61, pb^hs.PB/pb[+]

(Boube et al., 1997)

Ras85D^e1B/Ras85D[+] is a suppressor of antenna phenotype of RasGAP1^sxt-BJ61, pb^hs.PB

(Boube et al., 1997)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein phenotype of RasGAP1^sxt-BJ61, pb^hs.PB

(Boube et al., 1997)

Ras85D^e1B/Ras85D[+] is a suppressor of wing vein phenotype of RasGAP1^sxt-BJ61, pb^hs.PB/pb[+]

(Boube et al., 1997)

Ras85D^e1B/Ras85D[+] is a suppressor | partially of haltere phenotype of Df(3R)Ubx109/+, RasGAP1^sxt-BJ61

(Boube et al., 1997)

Ras85D^e1B is a suppressor of phenotype of rho^hs.sev

(Freeman, 1994)

Ras85D^e1B is a suppressor of eye phenotype of Src64B^{Δ540.hs.2sev}

(Kussick et al., 1993)

NOT Suppressor of

Statement

Reference

Ras85D^e1B is a non-suppressor of eye photoreceptor cell | ectopic phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Lee et al., 2002)

Ras85D^e1B is a non-suppressor of eye phenotype of PDZ-GEF^EP388, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF

(Lee et al., 2002)

Ras85D^e1B is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR

(Sullivan and Rubin, 2002)

Ras85D^e1B is a non-suppressor of phenotype of arm³

(Cox et al., 2000)

Ras85D^e1B is a non-suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

Ras85D^e1B is a non-suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

Ras85D^e1B is a non-suppressor of phenotype of sina^GMR.PN

(Neufeld et al., 1998)

Other

Statement

Reference

Bap170^C1.UAS, Ras85D^e1B, Scer\GAL4^hs.2sev has eye phenotype

(Rendina et al., 2010)

Bap170^C1.UAS, Ras85D^e1B, Scer\GAL4^hs.2sev has photoreceptor cell R7 phenotype

(Rendina et al., 2010)

Ras85D^e1B, da² has follicle cell phenotype

(Smith et al., 2002)

Ras85D^e1B/Ras85D[+], da² has follicle cell phenotype

(Smith et al., 2002)

Df(3R)Ubx109/+, RasGAP1^sxt-BJ61, Ras85D^e1B has haltere phenotype

(Boube et al., 1997)

RasGAP1^sxt-BJ61, Ras85D^e1B, pb^hs.PB/pb[+] has antenna phenotype

(Boube et al., 1997)

RasGAP1^sxt-BJ61, Ras85D^e1B, pb^hs.PB/pb[+] has wing vein phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L3 phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L4 phenotype

(Boube et al., 1997)

Ras85D^e1B, pb^hs.PB has wing vein L5 phenotype

(Boube et al., 1997)

Additional Comments

Genetic Interactions

Statement

Reference

Ras85D^e1B/+ rescues the lifespan defect of Uba1^B1/+ mutants, and partially rescues the lifespan defect of Uba1^B1/Uba1^B1 mutants.

(Liu and Pfleger, 2013)

Ras85D^e1B suppresses the increased size of Apc2^g10 Apc^Q8 mutant intestinal stem cell clones 7 and 14 days after clone induction (ACI). The multilayering phenotype often seen at 21 days ACI is also suppressed. This reduction in cell number is due to reduced proliferation, rather than increased cell death: TUNEL expression is unchanged and expression of the apoptotic protein BacA\p35 has no effect on the phenotype. The remaining increase in clone size seen in the Ras85D^e1B, Apc2^g10, Apc^Q8 triple mutant flies is fully suppressed upon expression of pan^ΔN.Scer\UAS under the control of Scer\GAL4^Act.PU, and no increase in apoptosis is seen in these flies.

(Wang et al., 2013)

The extra wing vein phenotype caused by expression of CG4096^KK108644 under the control of Scer\GAL4^Act5C.PU is significantly suppressed if the flies also carry Ras85D^e1B.

(Butchar et al., 2012)

Ras85D^e1B flies which are also expressing Bap170^C1.Scer\UAS under the control of Scer\GAL4^hs.2sev have rough eyes and lack all R7 photoreceptor cells.

(Rendina et al., 2010)

Ras85D^e1B dominantly suppresses the extra wing veins caused by constitutive expression of Rabex-5^VDRC.cUa via Scer\GAL4^Act.PU.

Ras85D^e1B dominantly suppresses the extra wing veins caused by wing-specific expression of Rabex-5^VDRC.cUa via Scer\GAL4^C-765.

A Ras85D^e1B/+ genetic background results in fewer flies with severe eye phenotypes upon co-expression of Rabex-5^VDRC.cUa and Dcr-2^Scer\UAS.cDa driven by Scer\GAL4^ey.PU.

Ras85D^e1B dominantly suppresses the increased body size, body length and wing area resulting from the expression of Rabex-5^VDRC.cUa driven by Scer\GAL4^Act.PU. Body weight of all flies and wing area in female flies are no longer statistically different from controls.

(Yan et al., 2010)

The presence of a Ras85D^e1B background significantly suppresses ectopic wing vein formation found in csw^{Y279C.Scer\UAS} (Scer\GAL4^tub) mutants.

A Ras85D^e1B background suppresses the csw^{Y279C.Scer\UAS} (Scer\GAL4^tub) rough eye phenotype, normalizing the numbers of R7 and ommatidial rotation.

(Oishi et al., 2009)

The cell-autonomous overgrowth phenotype found in Uba1^B2 homozygous and Uba1^B1/Uba1^B2 trans-heterozygous eye discs is suppressed in a Ras85D^e1B heterozygous background.

A Ras85D^e1B/+ background dramatically suppresses the lethality found in Uba1^B1/Uba1^B2 homozygotes.

Uba1^B1/Uba1^B2 eyes in a Ras85D^e1B heterozygous background show decreased suppression of egr^Scer\UAS.cMa (under the control of Scer\GAL4^GMR.PF) induced cell death.

Overall cell proliferation in Uba1^B1/Uba1^B2 eye discs is reduced in a Ras85D^e1B/+ background. Ectopic proliferation posterior to the morphogenetic furrow decreases and is restricted to a thin stripe.

A Ras85D^e1B/+ background dramatically suppresses the non-autonomous overgrowth phenotype found in Uba1^A1 mosaic eye clones.

(Yan et al., 2009)

Expression of S6kII^{K231R.Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^hs.2sev, causes a more severe loss of photoreceptor cells when expressed in a Ras85D^e1B/+ background.

Expression of S6kII^{Scer\UAS.T:Hsap\MYC}, under the control of Scer\GAL4^GMR.PF, causes a more severe roughened-eye phenotype and a greater loss of rhabdomeres when expressed in a rl^10a/+ background than when expressed in a wild-type background.

(Kim et al., 2006)

The ectopic wing vein phenotype caused by expression of Socs44A^Scer\UAS.cRa under the control of Scer\GAL4^en-e16E is suppressed by Ras85D^e1B/+.

(Rawlings et al., 2004)

Ras85D^e1B/+, S⁰⁵⁶⁷¹/+ double mutants have a stronger missing-bract phenotype than either mutant alone. (2% of bracts in the tibia and 60% in the basitarsus of the second leg remain). The addition of Ras85D^hs.M11.2 under heat shock control partially suppresses this phenotype.

(Held, 2002)

Ras85D^e1B/+ significantly restores the organisation of cysts and membrane deposition in the testes of homozygous Df(3R)rn20 males. These animals show reduced eclosion and have a short life span.

Ras85D^e1B/+ in a Df(3R)rn20 homozygous background results in a significant increase in the number of pigment cells and closer packing than seen in a Df(3R)rn20 single mutant background. The ommatidia remain strangely shaped and have variable numbers of outer and inner photoreceptor cells. There is a drastic reduction in the depth of the retina.

(Bergeret et al., 2001)

Strongly enhances the eye phenotype produced by activated arm constructs. (either arm^S44Y.GMR or arm^S56F.GMR).

(Freeman and Bienz, 2001)

Expression of both Tsc1^Scer\UAS.cTa and gig^Scer\UAS.cTa in the eye under the control of Scer\GAL4^ey.PH results in a much smaller eye than normal. Addition of one copy of Ras85D^e1B enhances the phenotype.

(Tapon et al., 2001)

Dominantly suppresses the eye phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^GMR.PF. Completely dominantly suppresses the wing vein phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^dpp.blk1.

(Ishimaru et al., 1999)

Enhances the phenotype of E2f^GMR.PD Dp^GMR.PD BacA\p35^GMR.PH flies.

(Staehling-Hampton et al., 1999)

Dominantly suppresses the ability of Src42A^Su(phl)1-1 to suppress the lethality of phl¹/Y flies.

(Zhang et al., 1999)

The lethality caused by sev^{S11.T:Hsap\MYC} in combination with Ras85D^V12.sev is suppressed by Ras85D^e1B.

(Maixner et al., 1998)

pb^hs.PB Ras85D^e1B double heterozygotes have a partially penetrant truncation of wing veins L4 and L5, and the frequency of ectopic bristle formation on the distal end of wing vein L3 (seen in pb^hs.PB heterozygotes) is increased to 90% for females and 50% for males. The enhancement of the pb^hs.PB antenna to maxillary transformation phenotype caused by Gap1^sxt-BJ61 is reduced if the flies also carry one copy of Ras85D^e1B. The enhancement of the Gap1^sxt-BJ61 ectopic wing vein phenotype caused by pb^hs.PB is reduced if the flies also carry one copy of Ras85D^e1B. The enhancement of the Df(3R)Ubx109/+ phenotype by Gap1^sxt-BJ61 is partially reversed if the flies also carry Ras85D^e1B.

(Boube et al., 1997)

Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

(Hariharan et al., 1995)

Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling.

(Simon et al., 1991)

Xenogenetic Interactions

Statement

Reference

Heterozygosity for Ras85D^e2F, Ras85D^e1B or Ras85D⁰⁶⁶⁷⁷ suppresses the larval muscle phenotype seen in animals expressing Hsap\FOXO1A::Hsap\PAX7^Scer\UAS.cGa under the control of Scer\GAL4^Mhc.PW and also results in viable adult escapers.

(Galindo et al., 2006)

Complementation and Rescue Data

Rescued by

Ras85D^e2F/Ras85D^e1B is rescued by Ras85D^+t12

(Simon et al., 1991)

Partially rescued by

Ras85D^e1B is partially rescued by Ras85D^hs.M11.2

(Held, 2002)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

5689

sev¹⁴; Ras85D^e1B/TM3, Sb¹

Kyoto

108366

sev¹⁴; Ras85D^e1B/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (24)

Reported As

Symbol Synonym

E(e.1.B)

(Shamloula et al., 2002)

E(e1.b)

(Shamloula et al., 2002)

E(sev)3C^e1B

(Doyle and Bishop, 1993, Simon et al., 1991)

E(sev)3C^elb

(Boulton et al., 2000, Staehling-Hampton et al., 1999)

Ras1^E1B

(Bergeret et al., 2001, Maixner et al., 1998)

Ras1^E62K

(Yakoby et al., 2008)

Ras1^e1B

(Yang et al., 2016, Walker et al., 2006, Fetchko et al., 2002, Williams et al., 2001, Ishimaru et al., 1999, Boube et al., 1997, Li et al., 1997, The et al., 1997, Schweitzer et al., 1995, Kussick et al., 1993)

Ras1^e1b

(Zhang et al., 1999, Neufeld et al., 1998, Freeman, 1994)

Ras85^e1B

(Adamson and Lajeunesse, 2012)

Ras85D^E62K

(Zartman et al., 2008, Ward and Berg, 2005, Schnorr and Berg, 1996)

Ras85D^e1B

(Wolfstetter et al., 2020, Johns et al., 2018, Rives-Quinto et al., 2017, Wang et al., 2013, Butchar et al., 2012, Xu et al., 2011, Oishi et al., 2009, Yan et al., 2009, Tong et al., 2007, Galindo et al., 2006, Oishi et al., 2006, Rawlings et al., 2004)

Ras85D^e1b

(Brumby et al., 2004)

Ras¹

(Pallavi and Shashidhara, 2003)

Ras^e1B

(Hannan et al., 2006)

Ras^e1b

(Liu and Pfleger, 2013, Yan et al., 2010, Kim et al., 2006, Kim et al., 2004, Lee et al., 2002)

ras1^E1B

(Tapon et al., 2001)

ras1^e1B

(Huang and Fischer-Vize, 1996)

ras1^e1b

(Daga et al., 1996)

ras85B^e1B

(McEwen et al., 2000)

ras85D^e1B

(Cox et al., 2000)

ras85DelB

(Wu et al., 2009)

ras^E1FB

(Galindo et al., 2006)

ras^e1B

(Wang et al., 2013, Rendina et al., 2010, Reich and Shilo, 2002, Freeman and Bienz, 2001)

ras^e1b

(Gafuik and Steller, 2011)

Name Synonyms

Secondary FlyBase IDs

References (62)

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