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General Information
Symbol
Dmel\Ras85De2F
Species
D. melanogaster
Name
FlyBase ID
FBal0030102
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
Ras1e2f, rase2F, E(sev)3CE2F, Ras1D38N, Dras1e2f
Key Links
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Nucleotide change:

    G9512180A

    Amino acid change:

    D38N | Ras85D-PA

    Reported amino acid change:

    D38N

    Comment:

    Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Missense mutation.

    Amino acid replacement: D38N.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 1 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Disease-implicated variant(s)
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    The concentration of circulating hemocytes in Ras85De2F heterozygous third instar larvae is comparable to that in wild-type controls.

    The number of eve-positive pericardial progenitor cell clusters is markedly reduced in mutant stage 10 embryos.

    Heterozygous Ras85De2F flies exhibit normal life spans.

    The adult eyes of Ras85D2F4/Ras85De2F transheterozygotes have some clusters that are missing one or more photoreceptors. Misrotation of clusters is not seen.

    Homozygous somatic clones in the legs and wings have bractless mechanosensory bristles.

    Ras85DC33/Ras85De2F survive to the third larval instar stage.

    94% of eggs laid by Ras85De2F/Ras85Dix12a females have one fused dorsal appendage.

    Homozygous embryos show a loss of 3 chordotonal organs per abdominal hemisegment (VChA and two in LCh5).

    Embryos show reduced number of midline glial cells.

    Heterozygous loss of Ras85D has no effect on size of Nf1 mutant pupae.

    Removal of one copy of dos from sev::tor13D:Y2546F.hs.sev individuals causes a significant reduction in ommatidia with multiple R7 cells.

    Ras85De2F/Ras85D05703 transheterozygotes are semi-lethal. Female transheterozygotes with Ras85D05703 lay eggs with a severely disrupted eggshell (patch of chorion instead of dorsal appendages), 57% of eggs hatch and develop to normal larvae. Unhatched embryos have normal cuticles, or are missing elements of the head skeleton or have defects in the anterior and posterior termini, holes in the cuticle and disordered posterior segments. Transheterozygotes with Ras85D05703 exhibit slightly disrupted ommatidial array (small number of fused and misplaced ommatidia).

    Clonal analysis reveals phenotypes in the adult including loss of wing vein, ectopic wing vein, reduced cell size, extra bristles, cell lethality and (incompletely expressed) tergite bristle abnormalities.

    Reduces the ability of a cell to develop as an R7 cell or as any other photoreceptor.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhancer of
    Statement
    Reference
    NOT Enhancer of
    Statement
    Reference
    Suppressor of
    NOT Suppressor of
    Statement
    Reference
    Phenotype Manifest In
    Suppressed by
    Enhancer of
    Statement
    Reference

    Ras85De2F is an enhancer of ommatidium phenotype of S48-5

    Ras85De2F is an enhancer of eye phenotype of rprGMR.PW

    Ras85De2F is an enhancer of eye phenotype of hidGMR.PG/WGMR.PG

    Ras85De2F is an enhancer of eye phenotype of rux3

    NOT Enhancer of
    Statement
    Reference

    Ras85De2F is a non-enhancer of eye phenotype of Pp2B-14Dact.GMR

    Ras85De2F is a non-enhancer of phenotype of Doa7/DoaHD

    Ras85De2F is a non-enhancer of phenotype of DoaHD/DoaDem

    Suppressor of
    Statement
    Reference

    Ras85De2F/Ras85D[+] is a suppressor | partially of wing vein | ectopic phenotype of sl2

    Ras85De2F/Ras85D[+] is a suppressor of eye | somatic clone phenotype of gfr1

    Ras85De2F/Ras85D[+] is a suppressor of wing vein L2 phenotype of Rabex-5VDRC.cUa, Scer\GAL4Act.PU

    Ras85De2F/Ras85D[+] is a suppressor of phenotype of Src42ASu(Raf)1-1

    Ras85De2F/Ras85D[+] is a suppressor of phenotype of aos257

    Ras85De2F is a suppressor of phenotype of Src42AKR.hs.2sev

    Ras85De2F is a suppressor of phenotype of svp1.sev

    Ras85De2F is a suppressor of phenotype of rhohs.sev

    Ras85De2F is a suppressor of eye phenotype of Src64BΔ540.hs.2sev

    NOT Suppressor of
    Statement
    Reference

    Ras85De2F is a non-suppressor of eye phenotype of Pp2B-14Dact.GMR

    Ras85De2F is a non-suppressor of ommatidium phenotype of Rac1V12.hs.sev

    Ras85De2F is a non-suppressor of phenotype of Doa7/DoaHD

    Ras85De2F is a non-suppressor of phenotype of DoaHD/DoaDem

    Ras85De2F is a non-suppressor of phenotype of dshhs.sev.B

    Additional Comments
    Genetic Interactions
    Statement
    Reference

    The increased concentration of circulating hemocytes characteristic for Graf1/Y mutant third instar larvae is suppressible by combination with a single copy of Ras85De2F.

    The loss of eve-positive pericardial progenitor cell clusters which is seen in stage 10 Ras85De2F embryos is partially rescued if the embryos are also mutant for Df(3R)Espl22.

    One copy of Ras85De2F partially suppresses the ectopic wing vein phenotype seen in sl2 homozygotes.

    The ommatidial patterning defects seen in homozygous gfr1 clones in the pupal eye disc are not suppressed by Ras85De2F.

    The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr1 is suppressed by Ras85De2F/+.

    Ras85De2F dominantly suppresses the extra wing veins caused by constitutive expression of Rabex-5VDRC.cUa via Scer\GAL4Act.PU.

    S48-5/Ras85De2F mutants show 58.05%+-4.57 misrotated ommatidia compared to 9.55%+-1.43 seen in S48-5 mutants alone.

    The wing phenotype caused by expression of vapScer\UAS.T:Hsap\MYC under the control of Scer\GAL4Bx-MS1096 is not enhanced by one copy of Ras85De2F. Suppresses the extra wing vein phenotype caused by htl::tor4021.Scer\UAS expressed under the control of Scer\GAL4Bx-MS1096.

    The fused dorsal appendage phenotype is partially suppressed by one copy of Src42A15-1 or Df(2R)nap8.

    Dominantly suppresses the ability of Src42ASu(phl)1-1 to suppress the lethality of phl1/Y flies.

    Ras85De2F significantly suppresses the formation of extra outer photoreceptors caused by B-H1sev.PH. The fraction of B-H1sev.PH ommatidia that lack 1 or 2 outer photoreceptors is increased by Ras85De2F.

    Enhances the eye reduction phenotype caused by rprGMR.PW. Enhances the eye reduction phenotype caused by WGMR.PG.

    fs(1)K101/fs(1)K101; Ras85Dix13b/Ras85De2F females lay eggs that range from strongly dorsalised to weakly ventralised, indistinguishable from Ras85D mutant eggs. The embryos range from strongly dorsalised to weakly dorsalised, to almost normal with slight head defects.

    Suppressor of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

    Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}.

    Suppression of the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and no effect on the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia).

    Enhances the sevB4 phenotype: disrupts sevB4 protein signalling.

    Xenogenetic Interactions
    Statement
    Reference

    Heterozygosity for Ras85De2F, Ras85De1B or Ras85D06677 suppresses the larval muscle phenotype seen in animals expressing Hsap\FOXO1A::Hsap\PAX7Scer\UAS.cGa under the control of Scer\GAL4Mhc.PW and also results in viable adult escapers. Of these three alleles, Ras85De2F is the strongest suppressor: at the lower temperature of 23 oC, 83% of Hsap\FOXO1A::Hsap\PAX7Scer\UAS.cGa, Scer\GAL4Mhc.PW, Ras85De2F/+ larvae are rescued to adult viability whereas only 15% of Hsap\FOXO1A::Hsap\PAX7Scer\UAS.cGa, Scer\GAL4Mhc.PW larvae survive to adulthood.

    Complementation and Rescue Data
    Comments
    Images (0)
    Mutant
    Wild-type
    Stocks (2)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (18)
    References (61)