FB2025_01 , released February 20, 2025
Allele: Dmel\Rb97D1
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General Information
Symbol
Dmel\Rb97D1
Species
D. melanogaster
Name
FlyBase ID
FBal0030104
Feature type
allele
Associated gene
Dmel\Rb97D
Associated Insertion(s)
P{PZ}Rb97D1
Carried in Construct
Key Links
Genomic Maps

Allele class

loss of function allele

Mutagen
Nature of the Allele
Allele class

loss of function allele

(Karsch-Mizrachi and Haynes, 1993)
Mutagen
P-element activity
(Heatwole and Haynes, 1996, Castrillon et al., 1993)
Delta2-3 transposase
(Karsch-Mizrachi and Haynes, 1993)
Progenitor genotype
Associated Insertion(s)
P{PZ}Rb97D1
Cytology
Description

Insertion of P{ry+lacZ} into the 5' untranslated sequences of Hrb97D.

(Karsch-Mizrachi and Haynes, 1993)

Rb97D gene product has been isolated and characterised, the protein contains two copies of the RNP sequence in the amino half and a proline rich domain in the carboxyl half. The protein is encoded by multiple transcripts that are expressed throughout development.

(Karsch-Mizrachi and Haynes, 1992)
Allele components
Component
Use(s)
Inserted element
P{PZ}
Encoded product / tool
lacZ
Tagged with
Tag:NLS(P)
Mutations Mapped to the Genome
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      nucleus & spermatid

      (Raja and Renkawitz-Pohl, 2005)
      Detailed Description
      Statement
      Reference

      Rb97D1 spermatids show defective nuclear shaping although chromatin condensation does occur.

      (Raja and Renkawitz-Pohl, 2005)

      Gross morphology of male reproductive tract appears normal, but sperm are completely absent from seminal vessels, suggesting a defect in spermatogenesis itself. Testes of Rb97D1 males contain spermatogonia, primary spermatocytes and elongating spermatids, but few individualized sperm, suggesting that the mutation leads to a failure of spermatogenesis beyond individualization and coiling stages. The base of the testis near the duct leading to the seminal vesicle contains refractile material, apparently degenerating sperm.

      (Karsch-Mizrachi and Haynes, 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Comments
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer

      A. Spradling.

      Comments
      Comments

      Precise excision revertants of Rb97D1 restore fertility completely.

      (Karsch-Mizrachi and Haynes, 1993)
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (7)
      Reported As
      Symbol Synonym
      RB97D1
      (Ustaoglu et al., 2019)
      Rb97D03445
      Rb97D1
      (Christensen et al., 2008.4.15, Christensen et al., 2008.6.11, Raja and Renkawitz-Pohl, 2005)
      Rb97D3
      ms(3)03445
      (Spradling et al., 1999)
      ms(3)0344503445
      rb97d1
      (Sofola et al., 2007)
      Name Synonyms
      Secondary FlyBase IDs
      • FBal0028527
      References (14)