Allele Dmel\Rh4^ninaE.P

General Information
Symbol	Dmel\Rh4^ninaE.P	Species	D. melanogaster
Name		FlyBase ID	FBal0030115
Feature type	allele	Associated gene	Dmel\Rh4

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Colley et al., 1991, Feiler et al., 1992, Ranganathan et al., 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference The entire Rh4 structural gene is under the control of the ninaE promoter. (Ranganathan et al., 1994) Construct: Entire gene and upstream sequences of Rh4 are fused to 2.8kb ninaE promoter fragment containing 67 nucleotides of untranslated leader sequence. (Feiler et al., 1992)
Carried in construct	P{Rh1+4} (Salcedo et al., 1999, Ahmad et al., 2006, Ranganathan et al., 1994, Feiler et al., 1992, Colley et al., 1991, Lee et al., 1996, Shen et al., 2011)
Cytology
Phenotypic Data
Phenotypic Class

Phenotype Manifest In

Detailed Description
	Statement Reference Whole cell patch clamp recordings of P{Rh1+4}; ninaE¹⁷ photoreceptors demonstrates light-dependent changes in intracellular calcium result from the influx of external calcium. Application of thapsigargin results in a steady increase of intracellular calcium (demonstrating the existance of IP₃-sensitive stores of calcium). Even after complete depletion of calcium the light response is not abolished. Instead the rise in intracellular calcium is associated with a partial desensitisation (adaptation) of the electrical response. There is no functional link between average intracellular calcium and adaptation recovery mechanisms. Localised intracellular calcium drives aspects of photoreceptor cell regulation. (Ranganathan et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement Reference Rh4^ninaE.P is a suppressor of thermotaxis behavior defective \| larval stage phenotype of ninaE¹⁷ (Shen et al., 2011)
Phenotype Manifest In
Suppressor of
Statement Reference Rh4^ninaE.P is a suppressor of phenotype of ninaE¹⁷ (Feiler et al., 1992)
Additional Comments
Genetic Interactions
Statement Reference Expression of Rh4[ninaE.P] rescues the ability of ninaE[17] larvae to strongly discriminate between 18[o]C and 24[o]C in a binary choice thermotaxis assay. (Shen et al., 2011)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	A UV sensitive rhodopsin transgene. (Ranganathan et al., 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	Rh4^ninaE.P Rh4^Rh1+4
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Shen et al., 2011, Science 331(6022): 1333--1336 Function of rhodopsin in temperature discrimination in Drosophila. [FBrf0213227] Ahmad et al., 2006, J. Biol. Chem. 281(14): 9205--9209 The role of Drosophila ninaG oxidoreductase in visual pigment chromophore biogenesis. [FBrf0190883] Salcedo et al., 1999, J. Neurosci. 19(24): 10716--10726 Blue- and green-absorbing visual pigments of Drosophila: ectopic expression and physiological characterization of the R8 photoreceptor cell-specific Rh5 and Rh6 rhodopsins. [FBrf0123183] Lee et al., 1996, Microsc. Res. Tech. 35(6): 418--430 Vitamin A, visual pigments, and visual receptors in Drosophila. [FBrf0091394] Ranganathan et al., 1994, Neuron 13(4): 837--848 Cytosolic calcium transients: spatial localization and role in Drosophila photoreceptor cell function. [FBrf0077008] Feiler et al., 1992, J. Neurosci. 12(10): 3862--3868 Ectopic expression of ultraviolet-rhodopsins in the blue photoreceptor cells of Drosophila: visual physiology and photochemistry of transgenic animals. [FBrf0056577] Colley et al., 1991, Cell 67: 255--263 The cyclophilin homolog ninaA is required in the secretory pathway. [FBrf0053360]

Allele Dmel\Rh4ninaE.P

Allele Dmel\Rh4^ninaE.P