Allele Dmel\Scm^ET50

General Information
Symbol	Dmel\Scm^ET50	Species	D. melanogaster
Name		FlyBase ID	FBal0030201
Feature type	allele	Associated gene	Dmel\Scm

Map ( GBrowse )
Allele class	hypomorphic allele - genetic evidence
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Bornemann et al., 1996, Bornemann et al., 1998)
Mutagen	ethyl methanesulfonate (Bornemann et al., 1996, Bornemann et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 3R:5,461,996..5,461,996 na_change=G5461996A pr_change=G275E\|Scm-PA reported_pr_change=G275E\|FBrf0104734 comment=Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change. evidence=experimental (Bornemann et al., 1998)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: G275E. (Bornemann et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	lethal \| pupal stage (with Scm^H1) (Peterson et al., 2004) lethal \| pupal stage \| recessive (Bornemann et al., 1998) lethal \| recessive (Wu et al., 1989, Bornemann et al., 1996) non-enhancer of variegation, with P{hsp26-pt-T}39C-5 (Boivin et al., 2003) non-suppressor of variegation, with P{hsp26-pt-T}39C-5 (Boivin et al., 2003)
Phenotype Manifest In
	sex comb \| ectopic (Wu et al., 1989)
Detailed Description
	Statement Reference Heterozygous males are viable and often show ectopic sex comb teeth. Mild suppressor of z¹ eye colour. (Wu et al., 1989)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement Reference Scm^ET50 is an enhancer of lethal \| dominant \| partially phenotype of Pc³ (Bornemann et al., 1998) Scm^ET50 is an enhancer of visible \| dominant \| homeotic phenotype of Pc³ (Bornemann et al., 1998)
Suppressor of
Statement Reference Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.10 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.13 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.13, w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.scs'.11 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^{Mcp.scs'.12:15} (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.scs'.13, w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of eye color defective phenotype of w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50 is a suppressor of eye color defective phenotype of P{iab-7PRE-w^enh.260}1, w^{enh.iab-7PRE.260} (Mishra et al., 2001) Scm^ET50 is a suppressor of eye color defective phenotype of P{iab-7PRE-w^enh.260}2, w^{enh.iab-7PRE.260} (Mishra et al., 2001) Scm^ET50 is a suppressor of eye color defective phenotype of P{iab-7PRE-w^enh.410}1, w^{enh.iab-7PRE.410} (Mishra et al., 2001) Scm^ET50 is a suppressor of eye color defective phenotype of P{iab-7PRE-w^enh.410}2, w^{enh.iab-7PRE.410} (Mishra et al., 2001)
NOT Suppressor of
Statement Reference Scm^ET50/Scm[+] is a non-suppressor of eye color defective phenotype of w^Mcp.11 (Muller et al., 1999)
Other
Statement Reference P{hsp26-pt-T}39C-5, Scm^ET50, ph-p⁴¹⁰ has suppressor of variegation phenotype (Boivin et al., 2003) Pc², Scm^ET50 has lethal \| dominant \| partially phenotype (Bornemann et al., 1998, Bornemann et al., 1998) Sce^D1, Scm^ET50 has lethal \| dominant \| partially phenotype (Bornemann et al., 1998, Bornemann et al., 1998) Scm^ET50, ph-p⁴⁰⁹ has lethal \| partially phenotype (Bornemann et al., 1998, Bornemann et al., 1998)
Phenotype Manifest In
Enhancer of
Statement Reference Scm^ET50 is an enhancer of phenotype of Pc¹⁵ (Bornemann et al., 1998)
Suppressor of
Statement Reference Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^Mcp.10 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^Mcp.13 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^Mcp.13, w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^{Mcp.scs'.12:15} (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^Mcp.scs'.13, w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50/Scm[+] is a suppressor of pigment cell phenotype of w^Mcp.scs'.14 (Muller et al., 1999) Scm^ET50 is a suppressor of phenotype of z¹ (Bornemann et al., 1998) Scm^ET50 is a suppressor of pigment cell phenotype of w^Mcp.scs'.11 (Muller et al., 1999)
NOT Suppressor of
Statement Reference Scm^ET50/Scm[+] is a non-suppressor of pigment cell phenotype of w^Mcp.11 (Muller et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference Scm^ET50 suppresses the pairing-dependent silencing of w^{enh.iab-7PRE.410} seen in flies homozygous for P{iab-7PRE-w^enh.410}1 or P{iab-7PRE-w^enh.410}2. Scm^ET50 suppresses the pairing-dependent silencing of w^{enh.iab-7PRE.260} seen in flies homozygous for P{iab-7PRE-w^enh.260}1 or P{iab-7PRE-w^enh.260}2. (Mishra et al., 2001) The addition of heterozygous Scm^ET50 can suppress the Pairing sensitive silencing effect seen in w^Mcp.10, w^Mcp.13, w^{Mcp.scs'.12:15}, w^Mcp.scs'.11 and w^Mcp.scs'.14. The addition of heterozygous Scm^ET50 has no effect on the Pairing sensitive silencing effect seen in w^Mcp.11. the addition of heterozygous Scm^ET50 strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.scs'.11 (P{Mcp-w#11}16/+, P{Mcp-w#11}102/+) double heterozygous insertions. The addition of heterozygous Scm^ET50 strongly suppresses the Pairing sensitive silencing effect seen in w^Mcp.scs'.14/ w^Mcp.13 (P{Mcp-w#14}AP-2^stummelbein/+, P{Mcp-w#13}116/+) double heterozygous insertions. (Muller et al., 1999) Dominantly enhances the homeotic phenotypes of Pc³/+ adults. Partially lethal in double heterozygous combination with Pc³, Pc² or Sce^D1. ph-p⁴⁰⁹ males that are also heterozygous for Scm^ET50 show partial lethality. (Bornemann et al., 1998)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Fails to complement	Scm⁴ (Bornemann et al., 1998)
Rescued by	Scm^ET50/Scm^H1 is rescued by Scm^{triple.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is rescued by Scm^{WT.T:Zzzz\FLAG} (Peterson et al., 2004)
Partially rescued by	Scm^ET50/Scm⁴ is partially rescued by Scm^Cas-Scm7 (Bornemann et al., 1996)
Not rescued by	Scm^ET50/Scm^H1 is not rescued by Scm^{G31D.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is not rescued by Scm^{I29T.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is not rescued by Scm^{K55E.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is not rescued by Scm^{M43Δ.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is not rescued by Scm^{M46R.T:Zzzz\FLAG} (Peterson et al., 2004) Scm^ET50/Scm^H1 is not rescued by Scm^{ΔPSM.T:Zzzz\FLAG} (Peterson et al., 2004)
Comments
Stocks ( 1 )
Bloomington	42685 Scm^ET50 e¹/TM3, Sb¹ Ser¹
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	Scm^ET50
Name Synonym
Secondary FlyBase IDs

References ( 7 )
Research paper	Peterson et al., 2004, Genetics 167(3): 1225--1239 Requirement for sex comb on midleg protein interactions in Drosophila polycomb group repression. [FBrf0179383] Boivin et al., 2003, Genetics 164(1): 195--208 Telomeric associated sequences of Drosophila recruit Polycomb-group proteins in vivo and can induce pairing-sensitive repression. [FBrf0158993] Mishra et al., 2001, Mol. Cell. Biol. 21(4): 1311--1318 The iab-7 Polycomb Response Element maps to a nucleosome-free region of chromatin and requires both GAGA and pleiohomeotic for silencing activity. [FBrf0132408] Muller et al., 1999, Genetics 153(3): 1333--1356 The mcp element from the Drosophila melanogaster bithorax complex mediates long-distance regulatory interactions. [FBrf0111986] Bornemann et al., 1998, Genetics 150(2): 675--686 Expression and properties of wild-type and mutant forms of the Drosophila sex comb on midleg (SCM) repressor protein. [FBrf0104734] Bornemann et al., 1996, Development 122(5): 1621--1630 The Drosophila Polycomb group gene Sex comb on midleg (Scm) encodes a zinc finger protein with similarity to polyhomeotic protein. [FBrf0086923] Wu et al., 1989, Mol. Gen. Genet. 218: 559--564 Homeosis and the interaction of zeste and white in Drosophila. [FBrf0050577]

Allele Dmel\ScmET50

Allele Dmel\Scm^ET50