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General Information
Symbol
Dmel\abd-AUAS.cGa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Greig
FlyBase ID
FBal0030434
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-abd-A, UAS-abdA
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UAS regulatory sequences drive expression of abd-A coding sequences.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

dendrite & dendritic arborising neuron, with Scer\GAL4ppk.PG

denticle belt & abdominal segment 9, with Scer\GAL469B

Detailed Description
Statement
Reference

The expression of abd-AScer\UAS.cGa under the control of Scer\GAL4prd.PU leads to ectopic oenocytes in Prd-positive thoracic segments, but not in Prd-positive abdominal segments, during stage 15 of embryogenesis, as compared to controls.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4rn.PU suppresses wing formation.

In embryos expressing abd-AScer\UAS.cGa under the control of Scer\GAL4wor.PA, thoracic neuroblast 3-3 (NB3-3T) fails to enter quiescence during mid-embryogenesis and its embryonic cell lineage is extended - NB3-3T generates an increased number of EL neurons compared to wild type.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4ppk.PG results in a reduction in both dendritic branching and total dendritic length in class IV dendrite arborisation (da) neurons in third instar larvae.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4221 has no effect on the dendrite arborisation patterns of class I da neurons.

When abd-AScer\UAS.cGa is driven by Scer\GAL4sd-SG29.1 abnormal wing vein patterns and incisions in the wing margin are seen.

Overexpression of abd-AScer\UAS.cGa under the regulation of Scer\GAL4sca-537.4 results in homeotic transformation of NB6-4t to NB6-4a.

The cardiac tube is thinner following abd-AScer\UAS.cGa overexpression during metamorphosis under the control of Scer\GAL4how-24B (using Scer\GAL80ts.╬▒Tub84B to block expression until the onset of metamorphosis) and myofibrils are longitudinal, instead of transversal as in the wild-type.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4dpp.blk1 results in transformation of the arista to tarsus.

In stage 16 abd-AScer\UAS.cGa; Scer\GAL4how-24B embryos, the number of cells in the dorsal vessel is increased from 104 to 116 cells compared to wild-type.

Embryos of both sexes expressing abd-AScer\UAS.cGa under the control of Scer\GAL4twi.PB initially contain male-specific somatic gonadal precursor (msSGP) cells. These disappear in females, and in males the join the posterior of the gonad as in wild type. Ectopic non-sexually dimorphic somatic gonadal precursor (SGP) cells are seen in more anterior regions of the embryos than normal.

Anterior dMP2 neurons do not survive in late embryos expressing abd-AScer\UAS.cGa under the control of Scer\GAL4Vap.P0201 (as occurs in wild-type embryos, where these neurons are lost by the late embryonic stage). Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4elav-C155 only results in a marginal increase in survival of anterior dMP2 neurons in late embryos compared to wild-type embryos (where these neurons are lost by the late embryonic stage).

In mutant embryos expressing abd-AScer\UAS.cGa driven by both Scer\GAL4twi.PG and Scer\GAL4how-24B ectopic cardioblasts are seen and the lymph glands are systematically eliminated and replaced by major pericardial cells. The anterior aorta is also transformed into a posterior aorta and heart tissue.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4sca-537.4 results in a mutant phenotype in the embryonic tritocerebrum. The phenotype has a penetrance of more than 95%.

The dch3 organs in segments T2 and T3 descend to the lateral cluster in embryos expressing abd-AScer\UAS.cGa under the control of Scer\GAL4da.G32. Their orientation is reversed compared to those in wild-type embryos and the ligament cells are found at their ventral edge.

When abd-AScer\UAS.cGa is driven by Scer\GAL4how-24B, the anterior of the dorsal vessel appears to have the same broader width and larger lumen characteristic of the heart, and the expression pattern of markers suggest ...

The aorta is transformed into heart in segments A5, A4 and part of A3 in embryos expressing abd-AScer\UAS.cGa under the control of Scer\GAL4twi.PG. Functional ostiae are seen in segments A4 and A3.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4lab.PH does not result in morphological defects in the tritocerebrum or any other part of the embryonic brain.

When driven by Scer\GAL4how-24B, shows a clear transformation of posterior pharyngeal wall to an abdominal character. Two distinct rows of abdominal denticles are found on this structure.

When driven by Scer\GAL469B, Scer\GAL4prd.RG1, or Scer\GAL4l(3)31-1-31-1 leads to a significant loss in viability.

Scer\GAL4twi.PG induced expression causes ectopic gonadal mesoderm formation (but no pole cells) in anterior segments of the embryo. Pole cells fail to condense normally and remain distributed within an elongated gonadal sheath.

With Scer\GAL469B, posterior spiracles are reduced to half their wild type size, in most embryos a small A9 denticle belt is formed anterior to the anal plates and the anal tuft is often reduced. Similar but weaker effects on the posterior spiracles are seen with Scer\GAL4h-1J3.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

The co-expression of abd-AScer\UAS.cGa and rhoScer\UAS.cUa under the control of Scer\GAL4prd.PU enhances the increased and/or ectopic oenocytes in Prd-positive segments during stage 15 of embryogenesis displayed in either single expression condition.

Co-expression of pbScer\UAS.T:Avic\GFP-EGFP suppresses the arista-to-tarsus transformation seen in flies expressing abd-AScer\UAS.cGa under the control of Scer\GAL4dpp.blk1.

Embryos coexpressing abd-AScer\UAS.cGa and Abd-BScer\UAS.cCa under the control of Scer\GAL4twi.PB have ectopic male-specific somatic gonadal precursor (msSGP) cells and ectopic somatic gonadal precursor (SGP) cells. A large group of SGPs and msSGPs associates with most of the germ cells in the region where the gonad would normally be coalescing and ectopic SGPs and msSGPs also associate with one another in more anterior regions.

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4lab.PH rescues the tritocerebral defects seen in lab14 embryonic brains. 28.4% of embryos show a complete rescue of the defects (taking into account that the phenotypic penetrance of the lab14 phenotype is 88.6%).

Scer\GAL4twi.PG induced expression of Abd-BScer\UAS.cCa blocks formation of the gonadal primordium, coexpression of Abd-BScer\UAS.cCa and abd-AScer\UAS.cGa restores gonadal mesoderm. Df(3R)Ubx109/Df(3R)P9 embryos and Df(3R)P9 homozygotes lack gonads, ectopic expression of abd-A restores the formation of an encapsulated gonad.

The spiracle phenotype of Df(3R)Ubx109 is modified in the abd-AScer\UAS.cGa, Scer\GAL4Kr.PM combination to suppress spiracle formation, irreversibly, wherever the ectopic protein is produced, i.e. completely in parasegments 7 and 8 and incompletely in parasegments 5,6 and 9. The phenotype of Df(3R)Ubx109 is modified in the abd-AScer\UAS.cGa, Scer\GAL4h-1J3 combination to suppress anterior spiracles in alternate parasegments. In the abd-AScer\UAS.cGa, Scer\GAL469B anterior spiracles are suppressed in all segments. The sensory organ phenotype of Df(3R)Ubx109 is modified in the abd-AScer\UAS.cGa, Scer\GAL4h-1J3 or abd-AScer\UAS.cGa, Scer\GAL4Kr.PM combination to prevent the ventral migration of the cells that would form the kolbchen, though later differentiation produces misplaced kolbchen rather than dorsal hairs. The sensory organ phenotype of Df(3R)Ubx109 is modified in the abd-AScer\UAS.cGa, Scer\GAL469B combination to affect the migration and differentiation of the kolbchen.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of abd-AScer\UAS.cGa under the control of Scer\GAL4abd-A-HC7JA1-GAL4 rescues the morphology of the denticle belts in abdominal segments A2 and A3 of abd-AHC7JA1-GAL4 homozygous embryos.

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Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
abd-AScer\UAS.cGa
abd-AUAS.cGa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Greig
Secondary FlyBase IDs
    References (35)