Allele Dmel\abd-A^hs.PS

General Information
Symbol	Dmel\abd-A^hs.PS	Species	D. melanogaster
Name	heat shock construct of Sampedro	FlyBase ID	FBal0030435
Feature type	allele	Associated gene	Dmel\abd-A

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Gonzalez-Reyes et al., 1992, Sanchez-Herrero et al., 1994, Moore et al., 1998)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference abd-A cDNA, including 5' leader and 3' untranslated sequences, is under control of an inducible Hsp70 promoter. (Sanchez-Herrero et al., 1994) Construct: abd-A driven by an Hsp70 promoter. (Gonzalez-Reyes et al., 1992)
Carried in construct	P{HS-abd-A} (Capovilla et al., 1994, Bello et al., 2003, Cenci and Gould, 2005, Moore et al., 1998, Merabet et al., 2002, Sanchez-Herrero et al., 1994, Heemskerk and DiNardo, 1994, Prokop and Technau, 1994, Boyle and DiNardo, 1995, Chauvet et al., 2000, Gonzalez-Reyes et al., 1992, Keegan et al., 1997, Haerry and Gehring, 1997, Boyle et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	increased cell death (Cenci and Gould, 2005) lethal \| larval stage \| recessive (Sanchez-Herrero et al., 1994)
Phenotype Manifest In
	abdominal neuroblast \| larval stage (Bello et al., 2003) abdominal segment 1 (Sanchez-Herrero et al., 1994) abdominal segment 2 \| ectopic (Chauvet et al., 2000) A-subperineurial glial cell \| ectopic (Prokop and Technau, 1994) B-subperineurial glial cell of abdomen \| ectopic (Prokop and Technau, 1994) head (Sanchez-Herrero et al., 1994) larval abdomen & neuroblast (Cenci and Gould, 2005) mesothoracic segment (Prokop and Technau, 1994) thorax (Sanchez-Herrero et al., 1994)
Detailed Description
	Statement Reference Induction of abd-A^hs.PS expression at 48 hours leads to the elimination of all abdominal neuroblasts by 72 hours. (Cenci and Gould, 2005) Expression of abd-A^hs.PS using a heat shock at 48-52 hours after larval hatching results in a 50% reduction in size in all three abdominal postembryonic neuroblast lineages compared to controls. (Bello et al., 2003) Ubiquitous expression of abd-A^hs.PS transforms anterior segments into extra A2 segments. (Chauvet et al., 2000) When heat shocked 4 hours after egg laying, a complete or almost complete pattern of ectopic A- and B- glial cells in T1-T3 develops. Heat shocks at 7 hours after egg laying have no effect. (Prokop and Technau, 1994) Heat induced expression between 3 and 5 hours of development causes transformation of thoracic segments to A2-A6 identity. Transformation of the first abdominal segment to a more posterior abdominal segment, A2-A6 identity. Appearance of two segments on the head as shown be the denticle belts. Posterior structures are not transformed. Same transformation is seen when coexpressed with Antp^hs.PHTA. Heat induced expression at the end of the third larval period or the beginning of pupation causes transformation of the first abdominal segment into a more posterior segment identity. Coexpression with Ubx^hs.PG shows similar transformation to expression of abd-A^hs.PS alone, thought it is difficult to ascertain the phenotypic result as abd-A^hs.PS intermediate transformations resemble those of Ubx^hs.PG embryos. Coexpression with Dfd^hs.PK suggests phenotypic suppression of Dfd by abd-A is dependent on gene dosage. (Sanchez-Herrero et al., 1994)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference abd-A^hs.PS has increased cell death phenotype, suppressible by grh³⁷⁰ (Cenci and Gould, 2005)
Phenotype Manifest In
Suppressed by
Statement Reference abd-A^hs.PS has larval abdomen & neuroblast phenotype, suppressible by grh³⁷⁰ (Cenci and Gould, 2005)
Additional Comments
Genetic Interactions
Statement Reference In contrast to when abd-A^hs.PS expression is induced at 48 hours in a wild-type background, this induction fails to cause apoptosis of abdominal neuroblasts in a grh³⁷⁰ background. (Cenci and Gould, 2005)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	abd-A^hs.PS
Name Synonym	heat shock construct of Sampedro
Secondary FlyBase IDs
	FBal0039122
References ( 15 )
Research paper	Cenci and Gould, 2005, Development 132(17): 3835--3845 Drosophila Grainyhead specifies late programmes of neural proliferation by regulating the mitotic activity and Hox-dependent apoptosis of neuroblasts. [FBrf0187453] Bello et al., 2003, Neuron 37(2): 209--219 A pulse of the Drosophila Hox protein Abdominal-A schedules the end of neural proliferation via neuroblast apoptosis. [FBrf0156017] Merabet et al., 2002, Genetics 162(1): 189--202 A green fluorescent protein reporter genetic screen that identifies modifiers of Hox gene function in the Drosophila embryo. [FBrf0152032] Chauvet et al., 2000, Proc. Natl. Acad. Sci. U.S.A. 97(8): 4064--4069 Distinct Hox protein sequences determine specificity in different tissues. [FBrf0127519] Moore et al., 1998, Development 125(5): 837--844 Gonadal mesoderm and fat body initially follow a common developmental path in Drosophila. [FBrf0101964] Boyle et al., 1997, Development 124(5): 971--982 Expression and function of clift in the development of somatic gonadal precursors within the Drosophila mesoderm. [FBrf0092473] Haerry and Gehring, 1997, Dev. Biol. 186(1): 1--15 A conserved cluster of homeodomain binding sites in the mouse Hoxa-4 intron functions in Drosophila embryos as an enhancer that is directly regulated by Ultrabithorax. [FBrf0093491] Keegan et al., 1997, Mech. Dev. 63(2): 145--157 A sequence conserved in vertebrate Hox gene introns functions as an enhancer regulated by posterior homeotic genes in Drosophila imaginal discs. [FBrf0095419] Boyle and DiNardo, 1995, Development 121(6): 1815--1825 Specification, migration and assembly of the somatic cells of the Drosophila gonad. [FBrf0078101] Capovilla et al., 1994, Cell 76(3): 461--475 Direct regulation of decapentaplegic by Ultrabithorax and its role in Drosophila midgut morphogenesis. [FBrf0068441] Heemskerk and DiNardo, 1994, Cell 76(3): 449--460 Drosophila hedgehog acts as a morphogen in cellular patterning. [FBrf0068521] Manak et al., 1994, Development 120(12): 3605--3619 Regulation of a decapentapleigic midgut enhancer by homeotic proteins. [FBrf0075105] Prokop and Technau, 1994, Development 120(9): 2567--2578 Early tagma-specific commitment of Drosophila CNS progenitor NB1-1. [FBrf0074206] Sanchez-Herrero et al., 1994, Mech. Dev. 46(3): 153--167 Developmental consequences of unrestricted expression of the abd-A gene of Drosophila. [FBrf0074351] Gonzalez-Reyes et al., 1992, Development 116(4): 1059--1068 Autocatalysis and phenotypic expression of Drosophila homeotic gene Deformed its dependence on polarity and homeotic gene function. [FBrf0055838]

Allele Dmel\abd-Ahs.PS

Allele Dmel\abd-A^hs.PS