|Name||heat shock construct of Sampedro||FlyBase ID||FBal0030435|
|Feature type||allele||Associated gene||Dmel\abd-A|
|Mutagen||in vitro construct - regulatory fusion|
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|Nature of the Allele|
|Mutations Mapped to the Genome|
|Associated Sequence Data|
|Nature of the lesion|
abd-A cDNA, including 5' leader and 3' untranslated sequences, is under control of an inducible Hsp70 promoter.
|Carried in construct|
(Capovilla et al., 1994, Bello et al., 2003, Cenci and Gould, 2005, Moore et al., 1998, Merabet et al., 2002, Sanchez-Herrero et al., 1994, Heemskerk and DiNardo, 1994, Prokop and Technau, 1994, Boyle and DiNardo, 1995, Chauvet et al., 2000, Gonzalez-Reyes et al., 1992, Keegan et al., 1997, Haerry and Gehring, 1997, Boyle et al., 1997)
|Phenotype Manifest In|
larval abdomen & neuroblast
Induction of abd-Ahs.PS expression at 48 hours leads to the elimination of all abdominal neuroblasts by 72 hours.
Expression of abd-Ahs.PS using a heat shock at 48-52 hours after larval hatching results in a 50% reduction in size in all three abdominal postembryonic neuroblast lineages compared to controls.
Ubiquitous expression of abd-Ahs.PS transforms anterior segments into extra A2 segments.
When heat shocked 4 hours after egg laying, a complete or almost complete pattern of ectopic A- and B- glial cells in T1-T3 develops. Heat shocks at 7 hours after egg laying have no effect.
Heat induced expression between 3 and 5 hours of development causes transformation of thoracic segments to A2-A6 identity. Transformation of the first abdominal segment to a more posterior abdominal segment, A2-A6 identity. Appearance of two segments on the head as shown be the denticle belts. Posterior structures are not transformed. Same transformation is seen when coexpressed with Antphs.PHTA. Heat induced expression at the end of the third larval period or the beginning of pupation causes transformation of the first abdominal segment into a more posterior segment identity. Coexpression with Ubxhs.PG shows similar transformation to expression of abd-Ahs.PS alone, thought it is difficult to ascertain the phenotypic result as abd-Ahs.PS intermediate transformations resemble those of Ubxhs.PG embryos. Coexpression with Dfdhs.PK suggests phenotypic suppression of Dfd by abd-A is dependent on gene dosage.
|Phenotype Manifest In|
|Complementation & Rescue Data|
|Stocks ( 0 )|
|Notes on Origin|
|External Crossreferences & Linkouts|
|Synonyms & Secondary IDs ( 2 )|
heat shock construct of Sampedro
|Secondary FlyBase IDs|
|References ( 15 )|