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General Information
Symbol
Dmel\apUGO35
Species
D. melanogaster
Name
FlyBase ID
FBal0030502
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
apUG035
Mutagen
    Nature of the Allele
    Mutagen
    Mutations Mapped to the Genome
     
    Type
    Location
    Additional Notes
    References
    Associated Sequence Data
    DNA sequence
    Protein sequence
     
     
    Progenitor genotype
    Cytology
    Nature of the lesion
    Statement
    Reference

    Small deletion removing first exon of the ap gene.

    P element has been imprecisely excised from the gene resulting in a deletion of 6 kb removing the first exon of the ap transcription unit.

    Expression Data
    Reporter Expression
    Additional Information
    Statement
    Reference
     
    Marker for
    Reflects expression of
    Reporter construct used in assay
    Human Disease Associations
    Disease Ontology (DO) Annotations
    Models Based on Experimental Evidence ( 0 )
    Disease
    Evidence
    References
    Modifiers Based on Experimental Evidence ( 0 )
    Disease
    Interaction
    References
    Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
     
    Phenotypic Data
    Phenotypic Class
    Phenotype Manifest In
    Detailed Description
    Statement
    Reference

    apUGO35/aprK568 mutant wing discs have no wing pouch and the adult flies do not develop any wings.

    apUGO35/+ flies have normal wings.

    Mutants show occasional losses and misarrangements of individual embryonic abdominal lateral transverse muscles.

    Homozygous apUGO35 mutant flies lack wings.

    apUGO35/Df(2R)nap1 transheterozygotes completely lack wings.

    Wing defects are seen in aploxP.MCP-PRE.MM/apUGO35 mutant flies, but they are significantly less severe than those seen in aploxP.MCP-PRE.MM homozygotes.

    Very mild wing defects are seen in Df(2R)DG/apUGO35 mutant flies, but they are significantly less severe than those seen in Df(2R)DG homozygotes.

    Very few wing defects are seen in apf00451/apUGO35 mutant flies.

    While both homozygotes completely lack wings, the majority of apUGO35/Df(2R)DG-Mcp transheterozygotes have crumpled wings.

    The dorsal/ventral boundary fails to form correctly in apUGO35/aprK568 mutant wing discs.

    Wing discs homozygous for apUGO35 have in general a profoundly altered morphology.

    In apUGO35/apmd544 the fourth tarsal segment is almost completely lost and is fused to the fifth. Other segments are normal.

    apmd544/apUGO35 wing discs do not form a smooth boundary between dorsal and ventral cells.

    The wing margin is reduced and the wing is considerably smaller than normal in apmd544/apUGO35 flies. When present, wing margin bristles have ventral identity.

    apmd544/apUGO35 fail to develop normal wings.

    Wing pouch is missing.

    Transheterozygotes with Df(2R)nap1 show fusion of tarsus 4 with 5, reduction and deformity or loss of tarsus 4. Tarsus four and five fusions appear with lower frequency and expressivity in apUGO35/apmd544 transheterozygotes.

    apmd544/apUGO35 flies have immature ovaries, are sterile, die within 24-72 hours of eclosion and have no wings.

    apmd544/apUGO35 flies show reduction of the wing and complete loss of wing margin. apmd544/apUGO35 wing discs have small wing pouches.

    apmd544/apUGO35 flies lack wings, halteres and the scutellum region of the notum. The flies are sterile and have a lifespan of 1-3 days after eclosion.

    The wing blade does not develop in homozygous flies.

    Stumps are present in place of the wing.

    Neurons of homozygous embryos carrying the P{apC-tau-lacZ} exhibit pathfinding and fasciculation defects: ap neurons that project their axons along abnormal pathways and fail to fasciculate with one another. The ap neurons still project anteriorly within the connectives.

    Clonal analysis, in the wing disc, shows that clones in the dorsal compartment that lack ap function show a cell-autonomous transformation to a ventral identity, and the fate of wild type dorsal cells is altered as a consequence of their proximity to the ap- cells in the clone.

    Wings and halteres absent. Homozygous apUGO35 flies do not express the ap transcript in the embryo, in the larval brain, or in the imaginal discs. Lack some dorsal thoracic bristles.

    External Data
    Interactions
    Show genetic interaction network for Enhancers & Suppressors
    Phenotypic Class
    Enhanced by
    Statement
    Reference

    apUGO35/Df(2R)DG has visible phenotype, enhanceable by za

    NOT Enhanced by
    Statement
    Reference

    apUGO35/Df(2R)DG has visible phenotype, non-enhanceable by za

    Suppressed by
    Enhancer of
    Statement
    Reference

    ap[+]/apUGO35 is an enhancer of visible | dominant phenotype of Bx1

    apUGO35 is an enhancer of visible | dominant phenotype of Bx1

    Suppressor of
    Statement
    Reference
    Other
    Phenotype Manifest In
    Enhanced by
    Statement
    Reference

    apUGO35/Df(2R)DG has wing phenotype, enhanceable by za

    NOT Enhanced by
    Statement
    Reference

    apUGO35/Df(2R)DG has wing phenotype, non-enhanceable by za

    Suppressed by
    Statement
    Reference

    apUGO35 has wing disc phenotype, suppressible | partially by HE31

    NOT suppressed by
    Enhancer of
    Statement
    Reference

    ap[+]/apUGO35 is an enhancer of wing margin phenotype of Bx2

    ap[+]/apUGO35 is an enhancer of wing margin phenotype of Bx1

    ap[+]/apUGO35 is an enhancer of tarsal segment 4 phenotype of BM2

    ap[+]/apUGO35 is an enhancer of pretarsus phenotype of BM2

    apUGO35 is an enhancer of wing phenotype of Bx1

    Suppressor of
    Statement
    Reference

    apUGO35 is a suppressor of scutellar bristle | ectopic phenotype of ChiE

    apUGO35 is a suppressor of thorax phenotype of ChiE

    Other
    Additional Comments
    Genetic Interactions
    Statement
    Reference

    In lmsS95, apUGO35 double mutant embryos most of the lateral transverse (LT) muscles form, though the number of hemisegments with only three or two LT muscles present is significantly higher than for each of the single mutants. These effects appear additive rather than interactive.

    z1 does not enhance the mild wing phenotypes seen in apUGO35/Df(2R)DG mutant flies.

    za slightly enhances the mild wing phenotypes in apUGO35/Df(2R)DG mutant flies.

    apUGO35/aprK568; HE31 and apUGO35/aprK568; Su(H)del47/Su(H)8 wing discs fail to form a dorsal/ventral compartment boundary.

    The severity of the Bx1/+ wing scalloping phenotype is enhanced by one copy of apUGO35.

    apUGO35/+ does not cause any visible leg phenotypes in combination with Chig371/+, Chig96.1/+ or Chie5.5/+. ap::ChiΔLID.ΔLIM.Scer\UAS.T:Hsap\MYC; Scer\GAL4ap-md544 suppresses fusion of tarsal segments 4 and 5 in apUGO35/apmd544 flies. ap::Lim3Scer\UAS.cOa; Scer\GAL4ap-md544 suppresses fusion of tarsal segments 4 and 5 in apUGO35/apmd544 flies. BM2/+ enhances the tarsal phenotype of apUGO35/apmd544 flies: tarsal segment 4 is completely absent and the joint between tarsal segments 3 and 5 is affected. The tarsal segment phenotype of BM2 hemizygous males is enhanced by apUGO35/+: in the resulting flies tarsal segments 4 and 5 are shorter and are fused in 61% of the legs.

    Expression of capsScer\UAS.cSa under the control of Scer\GAL4ap-md544 restores the smooth interface between dorsal and ventral cells in apmd544/apUGO35 wing discs. The boundary between the dorsal and ventral cells is still irregular in apmd544/apUGO35 wing discs expressing trnScer\UAS.cMa under the control of Scer\GAL4ap-md544. Expression of ConScer\UAS.cRa or fngEP3082 under the control of Scer\GAL4ap-md544 does not restore a smooth boundary between the dorsal and ventral cells in apmd544/apUGO35 wing discs.

    Expression of DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies does not restore outgrowth of the wing, although the few margin bristles seen in the dorsal surface of these wings have dorsal identity. Growth of the wing and wing margin formation can be restored in apmd544/apUGO35 flies expressing fngEP3082 under the control of Scer\GAL4ap-md544. Both surfaces of the wing differentiate ventral structures; the anterior wing margin and alula differentiate ventral bristles on both surfaces and the pattern of vein corrugation is ventral. Co-expression of fngEP3082 and DrScer\UAS.cIa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in dorsal differentiation in bristles of the dorsal anterior wing margin.

    apUGO35/apmd544 flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 do not have wing margin defects.

    apUGO35,HE31 double mutants have large wing pouches with no margin structures.

    The addition of this mutant to ChiE homozygotes suppresses the thoracic left and loss of bristle phenotype seen in these flies.

    Expression of SerScer\UAS.cSa under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies restores the wing margin and supports growth of the wing. Patches of ectopic wing margin are found in both surfaces of the rescued wings and overgrowth of the wing can be seen. Expression of fngEP3082 under the control of Scer\GAL4ap-md544 rescues the phenotype of apmd544/apUGO35 flies. Patches of ectopic wing margin form and can be associated with outgrowth of the wing. Groups of cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing SerScer\UAS.cSa under the control of Scer\GAL4ap-md544. The mispositioned patches of dorsal or ventral cells form relatively smooth interfaces with surrounding cells. Cells appear to violate the dorsoventral boundary in apmd544/apUGO35 wing discs which are also expressing fngEP3082 under the control of Scer\GAL4ap-md544. Expression of both SerScer\UAS.cSa and fngEP3082 under the control of Scer\GAL4ap-md544 in apmd544/apUGO35 flies results in well-developed wings which differentiate ventral features on both surfaces; the wing margin, veins and sense organs on the topologically dorsal surface have exclusively ventral identity.

    Scer\GAL4dpp.blk1-mediated expression of vgScer\UAS.cKa rescues loss of wing phenotype. Wing stem is displaced dorsally into the thorax. Scer\GAL4dpp.blk1-mediated expression of wgScer\UAS.cGa does not rescue loss of wing tissue.

    The wing phenotype of homozygous flies is partially rescued by SerScer\UAS.cSa, fngScer\UAS.cKa or DlScer\UAS.cHa expressed under the control of Scer\GAL4dpp.blk1.

    Scer\GAL4dpp.blk1-mediated expression of fngScer\UAS.cKa elicits an ectopic wing. Wing exhibits DV asymmetries and the margin is on the dorsal side. Mediated expression of SerScer\UAS.cSa elicits an ectopic wing. Wing consists of dorsal and ventral cells and the wing margin cells are on the dorsal side.

    Xenogenetic Interactions
    Statement
    Reference

    The apmd544/apUGO35 phenotypes are rescued by Hsap\LHX2Scer\UAS.cRa expressed under the control of Scer\GAL4ap-md544. apmd544/apUGO35 flies rescued by the expression of Hsap\LHX2Scer\UAS.cRa under the control of Scer\GAL4ap-md544 often develop one to two extra bristles in the scutellum.

    Complementation and Rescue Data
    Partially rescued by
    Not rescued by
    Comments

    Expression of two copies of apcDNA.DV+E partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. The wing margin fails to form.

    Expression of two copies of apcDNA.DV+E+P partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. Wing rescue is not perfect: a clear dorsoventral margin is observed.

    Expression of two copies of apcDNA.DV+P fails to rescue the wing loss seen in apUGO35/aprK568 mutant flies.

    Expression of two copies of apcDNA.E+P partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. The wing margin fails to form.

    Reduction and fusion of the fourth tarsal segment in apmd544/Df(2R)nap1 animals is rescued by apScer\UAS.cOa.

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    Mutant
    Wild-type
    Stocks (0)
    Notes on Origin
    Discoverer
    External Crossreferences and Linkouts ( 0 )
    Synonyms and Secondary IDs (4)
    References (37)