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General Information
Symbol
Dmel\aprK568
Species
D. melanogaster
Name
FlyBase ID
FBal0030504
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
ap-lacZ, apLacZ, aprK568-lacZ, aprk560, aplacZ, apterous-lacZ, ap-Z
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion of P element enhancer trap in the 5' untranslated leader of the ap mRNA.

The P Ecol\lacZ enhancer trap inserted into ap 42 bp upstream from the longest cDNA clone at cytological position 41F--43A2.

Insertion components
P{PZ}aprK568
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

apUGO35/aprK568 mutant wing discs have no wing pouch and the adult flies do not develop any wings.

Wing defects are seen in aploxP.MCP-PRE.MM/aprK568 mutant flies, but they are significantly less severe than those seen in aploxP.MCP-PRE.MM homozygotes.

Wing defects are seen in Df(2R)DG-Mcp/aprK568 mutant flies, but they are less severe than those seen in Df(2R)DG-Mcp homozygotes.

Mild wing defects are seen in Df(2R)DG/aprK568 mutant flies, but they are less severe than those seen in Df(2R)DG homozygotes.

The dorsal/ventral boundary fails to form correctly in apUGO35/aprK568 mutant wing discs.

The D/V compartment boundary of aprK568/apmd544 wing discs is highly irregular (in contrast to wild type). The resulting adult wings are almost completely eliminated and show no evidence of a wing margin.

Adults show defects in the direct flight muscles. Of the group of four muscles 51-54, only one or two develop. IFMs are reduced in width at the posterior attachment site and do not undergo complete splitting. Muscle size and volume is reduced.

In mosaic analysis of wing clones generated in larvae at 60hrs AEL no ap- clones were found that define the D/V boundary from the dorsal side. Despite a dorsal origin, ap- cells behave like ventral cells with respect to the lineage restriction. Ventrally generated ap- cells never cross into the dorsal compartment, but can define the D/V boundary from the ventral side. ap- cells which cross to the ventral side usually retain their ap expression. Dorsal ap- clones are commonly surrounded by regions of abnormal folding and outgrowth, including blister-like projections from the disc epithelium. This effect is especially pronounced near the hinge region. In rare cases the posterior wing blade is duplicated.

Complete loss of wing, retaining only a stump of wing hinge or an amorphous tissue mass.

Wings and halteres absent.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
NOT suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

Flies expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544 in a aprK568/apmd544 background have ventralised halteres. In addition, the halteres form dark-pigmented bristles that are similar to those formed at the wing margin.

apUGO35/aprK568; HE31 and apUGO35/aprK568; Su(H)del47/Su(H)8 wing discs fail to form a dorsal/ventral compartment boundary.

Expression of apScer\UAS.cMa under the control of Scer\GAL4vg.boundary partially rescues the loss of wing tissue seen in vgnull/vg83b27 flies.

A sharp boundary between dorsal and ventral cells is restored in aprK568/apmd544 wing discs expressing fngScer\UAS.cKa under the control of Scer\GAL4ap-md544. No violations of the compartment boundary are seen. The resulting adult flies have wings of roughly normal size with clearly demarcated wing margin. The rescued wing margin is ventralised; dorsal-specific sensory bristles are completely missing along the anterior margin and cells on both sides of the margin secrete ventral bristle types. The dorsal and ventral wing surfaces are not adhered to each other, resulting in a balloon-like structure. aprK568/apmd544 flies expressing fngEP3082 under the control of Scer\GAL4ap-md544 have wing margin defects.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescued by

aprK568/apUGO35 is partially rescued by apcDNA.E+P

Not rescued by
Comments

Expression of two copies of apcDNA.DV+E partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. The wing margin fails to form.

Expression of two copies of apcDNA.DV+E+P partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. Wing rescue is not perfect: a clear dorsoventral margin is observed.

Expression of two copies of apcDNA.DV+P fails to rescue the wing loss seen in apUGO35/aprK568 mutant flies.

Expression of two copies of apcDNA.E+P partially rescues the wing loss seen in apUGO35/aprK568 mutant flies. The wing margin fails to form.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
Comments
Comments

Phenotype almost as extreme as for a null allele.

Not a transcript null but resembles nulls in adult phenotype.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
  • FBal0030497
References (21)