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General Information
Symbol
Dmel\atoUAS.cJa
Species
D. melanogaster
Name
Saccharomyces cerevisiae UAS construct a of Jarman
FlyBase ID
FBal0030530
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
UAS-ato
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

UASt regulatory sequences drive expression of an ato cDNA fragment that includes 48bp of sequence 5' to the predicted initiation codon, the open reading frame and 8bp of sequence 3' to the predicted stop codon.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 1 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

abdominal basiconical sensillum dbd & larva & nerve terminal, with Scer\GAL4sca.PC

adult thorax & macrochaeta, with Scer\GAL4109

chordotonal organ & wing | ectopic, with Scer\GAL4dpp.blk1

macrochaeta & wing | ectopic, with Scer\GAL4dpp.blk1

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4109

sensory mother cell & dorsal mesothoracic disc, with Scer\GAL4ptc-559.1

Detailed Description
Statement
Reference

Somatic clones of type I neuroblasts expressing atoUAS.cJa under the control of Scer\GAL4αTub84B.PL exhibit symmetric cell divisions (indicating altered cell polarity) and a reduction in cell size in the optic lobe of third instar larvae.

Expression of atoScer\UAS.cJa driven by Scer\GAL4dpp.blk1 leads to neurogenesis (presence of ectopic sensory organ precursors) in third instar larval wing discs.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 results in loss of scutellar bristles.

Ectopic expression of atoScer\UAS.cJa anterior to the morphogenetic furrow, under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C does not lead to premature differentiation.

Expression of atoScer\UAS.cJa with Scer\GAL4sca-109-68 does not elicit overt eye/R8 defects at 24[o]C.

Flies expressing atoScer\UAS.cJa under the control of Scer\GAL4dpp.blk1 have ectopic sternopleural and scutellar bristles.

Clones that express atoScer\UAS.cJa behind the morphogenetic furrow of the eye disc, driven by Scer\GAL4GMR.PF, leads to disorganised clusters with many cells having upregulated adherens junction components.

atoScer\UAS.cJa; Scer\GAL4dpp.blk1 flies have large numbers of ectopic sensory bristles around the A-P compartment boundary of their wings, along with ectopic chordotonal organs.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 results in a rough eye phenotype. Some R8 twinning is seen in the mutant ommatidia.

When expression is driven in the PNS by Scer\GAL4sca.PC, the larval dbd neuron projection is shifted ventrally and from the medial to the intermediate longitudinal fascicle and, in pronounced cases, the dbd-characteristic ventral extension no longer occurs. Late remodelling of the dbd terminal also does not occur. The major CNS axon fascicles are wild type and the larvae hatch.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4SG18.1 or Scer\GAL4neur-GAL4-A101 does not affect the formation of the antennal glomeruli.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4ey.PH gives rise to animals in which most of the head and one or both eyes of reduced size are present.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 produce densely packed arrays of ectopic chordotonal organs. Misexpression of atoScer\UAS.cJa driven by Scer\GAL4h-1J3 results in expansion of lateral chordotonal organ clusters (usually 6-10 neurons instead of 5) but the neurons are of normal morphology.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4unspecified results in no or very few ectopic olfactory-like sensilla.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4ato.3.6 results in excessive branching in the lobula and the medulla as compared to wild type. The organisation of the ato-expressing dorsal cluster, its size and its position are unaffected in third larval instar and adult brains. The number of axons crossing the optic chiasma, but not their morphology, is wild type.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-537.4 slightly induces the formation of multiple dendritic neurons in embryos. A slight increase in the number of chordotonal neurons in the lateral region is also seen. Expression of atoScer\UAS.cJa under the control of Scer\GAL4hs.PB using a 10 minute heat shock just after puparium formation results in ectopic bristles in the wing.

When atoScer\UAS.cJa is driven by Scer\GAL4neur-GAL4-A101, Many of the basiconic and trichoid sensilla of the antenna are converted to a coeloconic lineage, leading to an increase in the latter type of sense organs. Glial cell number is significantly increased and these cells now appear at ectopic positions on the antenna.

When atoScer\UAS.cJa is driven by Scer\GAL4neur-GAL4-A101 a reduction is seen in basiconica and trichoidea and a concomitant increase in coeloconic sensilla. This appears to be a conversion of other sensilla to "ectopic coeloconica".

Flies expressing atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 have rough eyes, with many ommatidia containing inappropriate complements of photoreceptors and support cells (there can be an increase or a decrease in any specific cell type). This phenotype results from a combination of recruitment and R8 spacing defects. Ommatidial spacing is abnormal and there appear to be fused ommatidia. R8 cells are more dense, frequently juxtaposed (twinned) and unevenly spaced relative to one another. The phenotype is non-autonomous; the presence of supernumerary cells is not always correlated with ectopic ato expression within them. The ommatidia are no longer arranged in straight rows and ectopic photoreceptors infrequently differentiate in the interommatidial spaces. Supernumerary R7 photoreceptors are frequently seen in these flies.

Flies expressing low levels of atoScer\UAS.cJa under the control of Scer\GAL4109 have ectopic bristles on the scutellum, on the dorsocentral notum and wing vein L3. A small number of ectopic chordotonal organs are detected under the cuticle of the scutellum and vein L3. The wild-type chordotonal organs are hyperplastic, consisting of more aligned scolopidia or individual chordotonal unit organs. Flies expressing higher levels of atoScer\UAS.cJa under the control of Scer\GAL4hs.PB show only the ectopic chordotonal organ phenotype. In many places the formation of ectopic chordotonal organs coincides with bristle loss in these flies. In some cases, when expression is driven by Scer\GAL4109, external sense organs are malformed and are associated with scolopales, suggesting an incomplete transformation to chordotonal organs. External sense organs affected in this way include the sternitals of the ventral abdomen, the wing sense organs ACV and TSM1+2 and in extreme cases all other thoracic macrochaetae. Extra sensory organ precursors are seen in the wing discs of flies expressing atoScer\UAS.cJa under the control of Scer\GAL4109. Flies expressing atoScer\UAS.cJa under the control of Scer\GAL4ptc-559.1 are not viable and die as pharate adults with deformed wings and appendages. Ectopic sensory organ precursors are seen in the wing discs of these animals.

Scer\GAL4384-mediated expression causes duplication of the antenna sacculus in a position near to the normal sacculus.

Scer\GAL4hs.PB mediated expression causes a slightly irregular ommatidial pattern: ectopic R8 cells in several ommatidia due to transformation of all 2-3 cells within the R8 equivalence group. Multiple R8 cells are observed in ommatidia that were within the morphogenetic furrow at the time of heat shock.

Fifteen minute heat shock pulse during larval development allows spatially restricted rescue of ommatidia. A row of ommatidia is formed within the pigment cell patch, ommatidia are of variable size. Longer or multiple heat shocks result in pupal death.

When ato is ectopically expressed, large numbers of ectopic chordotonal organs develop.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Suppressor of
Other
Phenotype Manifest In
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Suppressor of
NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

Co-expression of smt3Scer\UAS.P\T.T:Zzzz\FLAG.T:Zzzz\His6 enhances the loss of scutellar bristles caused by expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68.

Approximately 81% of cells overexpressing both atoScer\UAS.cJa and daΔex1.Scer\UAS ahead of the morphogenetic furrow (under the control of Scer\GAL4Scer\FRT.Rnor\CD2.Act5C) differentiate into neurons.

Co-expression of atoScer\UAS.cJa almost completely suppresses the formation of eye tumours seen in 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) flies.

Co-expression of atoScer\UAS.cJa in 'eyeful' third instar eye discs (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) results in dramatically increased apoptosis, as measured by decay staining.

Co-expression of atoScer\UAS.cJa reduces ectopic proliferation in "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs.

Co-expression of atoScer\UAS.cJa restores the size and organization of "eyeful" (Scer\GAL4ey.PH DlScer\UAS.cDa, psqGS88A8 and lolaGS88A8) third instar eye discs.

Co-expression of bskDN.Scer\UAS in a atoScer\UAS.cJa 'eyeful' (Scer\GAL4ey.PH, DlScer\UAS.cDa, psqGS88A8, lolaGS88A8) background leads to a suppression of the inhibitory effects of atoScer\UAS.cJa on the 'eyeful' tumor phenotype, restoring tumor formation and enhancing metastasis.

The eye and photoreceptor defects caused by expression of E(spl)m8-HLHSD.Scer\UAS with Scer\GAL4sca-109-68 are suppressed by co-expression of atoScer\UAS.cJa.

Expressing atoScer\UAS.cJa under the control of Scer\GAL4GMR.PF fails to suppress the morphogenetic furrow phenotype of Ras85DΔC40B clones.

The rough eye phenotype and R8 twinning phenotype caused by expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 is dominantly enhanced by ed4.12.

When expression is driven by Scer\GAL4h-H10 in an roDOM mutant, the stop-furrow phenotype of roDOM is suppressed.

The reduction in number of sensilla basiconica seen in lz3 flies is not rescued by expression of atoScer\UAS.cJa under the control of Scer\GAL4hs.PB even though the number of sensilla coeloconica is increased.

Expression of Nintra.GS.Scer\UAS under the control of Scer\GAL4ato.3.6 results in a severe inhibition of axonal branching over the lobula and a complete failure of innervation of the medulla. This phenotype is not altered by coexpression of atoScer\UAS.cJa.

Egfrf3 suppresses the recruitment defects in the eye caused by expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68, although abnormal pattern formation still occurs. ro1, ptc5, hunspecified or Dl9P enhance the rough eye phenotype caused by expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68. hhAC or ciunspecified suppress the rough eye phenotype caused by expression of atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68. The eye is extremely disorganised when both atoScer\UAS.cJa and hhScer\UAS.cSa are expressed under the control of Scer\GAL4sca-109-68. Many R7 cells differentiate in sev14 flies expressing atoScer\UAS.cJa under the control of Scer\GAL4sca-109-68 (in contrast to sev14 flies which lack R7 photoreceptors). This rescue of R7 formation by atoScer\UAS.cJa expressed under the control of Scer\GAL4sca-109-68 is strongly suppressed if the flies are also carrying Egfrf3.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of atoScer\UAS.cJa under the control of Scer\GAL47 restores the formation of R8 photoreceptor cells and numerous ommatidia in ato1 flies.

Expression of atoScer\UAS.cJa under the control of Scer\GAL4SG18.1 does not rescue the lack of antennal glomeruli seen in ato1/Df(3R)p13 mutants.

The lobular innervation pattern defect of ato1/Df(3R)p13 flies is rescued by atoScer\UAS.cJa expressed under the control of Scer\GAL4ato.3.6.

Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Used to ectopically express ato via a heat shock Scer\GAL4 construct.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
Reported As
Symbol Synonym
atoScer\UAS.cJa
atoUAS.cJa
Name Synonyms
Saccharomyces cerevisiae UAS construct a of Jarman
Secondary FlyBase IDs
    References (47)