FlyBase Allele Report: Dmel\bi[omb-282]

General Information

Symbol

Dmel\bi^omb-282

Species

D. melanogaster

Name

FlyBase ID

FBal0030628

Feature type

allele

Associated gene

Dmel\bi

Associated Insertion(s)

Carried in Construct

Also Known As

omb²⁸², l(1)omb²⁸²

Key Links

Genomic Maps

JBrowse

Allele class

loss of function allele

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

loss of function allele

(Kopp and Duncan, 1997)

Mutagen

ethyl methanesulfonate

(Pflugfelder et al., 1990)

Progenitor genotype

Cytology

Description

Amino acid replacement: E347K.

(Sen et al., 2010)

A missense mutation approximately 19 residues into the T-box domain of bi.

(Sen et al., 2010)

Point mutation.

(Pflugfelder et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

X:4,429,473..4,429,473 [+]

Nucleotide change:

G4429473A

Amino acid change:

Reported amino acid change:

E347K

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

(Sen et al., 2010)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

abdominal sternite & trichome

(Kopp and Duncan, 1997)

abdominal tergite

(Kopp and Duncan, 1997)

abdominal tergite & macrochaeta

(Kopp and Duncan, 1997)

abdominal tergite & trichome

(Kopp and Duncan, 1997)

abdominal tergite | posterior

(Kopp and Duncan, 1997)

adult abdomen

(Kopp and Duncan, 1997)

adult abdominal segment 3

(Kopp and Duncan, 2002)

adult abdominal segment 3 | anterior | ectopic

(Kopp and Duncan, 2002)

adult abdominal segment posterior compartment

(Kopp and Duncan, 2002)

adult cuticle & abdominal segment | male

(Kopp et al., 2000)

cercus

(Gorfinkiel et al., 1999)

epiproct

(Gorfinkiel et al., 1999)

eye (with bi^omb-1)

(Tsai et al., 2015)

hypandrial bristle

(Gorfinkiel et al., 1999)

hypandrial hair

(Gorfinkiel et al., 1999)

hypandrium

(Gorfinkiel et al., 1999)

hypoproct

(Gorfinkiel et al., 1999)

ommatidium | adult stage | increased number (with bi^omb-1)

(Tsai et al., 2015)

surstylar long bristle | dorsal

(Gorfinkiel et al., 1999)

wing

(Kopp and Duncan, 1997, Lecuit et al., 1996)

wing (with bi^omb-Gal4)

(Lecuit et al., 1996)

wing | somatic clone

(Lecuit et al., 1996)

Detailed Description

Statement

Reference

Partial deletions of posterior compartment structures are sometimes seen in the abdomens of bi^omb-282 adult hemizygotes. The third abdominal segment undergoes a mirror-image anterior duplication.

(Kopp and Duncan, 2002)

Hemizygous males lack segmental pigment stripes but retain pigment in segments A5 and A6.

(Kopp et al., 2000)

Homozygous females cannot be obtained. In bi^omb-282/bi^omb-282 male pharate adults, the dorsal bristles of the claspers and the hypandrium bristles are absent. Also the hypandrium is devoid of hairs and the hypandrium fragma is reduced. The anal plates are usually somewhat enlarged in the ventral region and reduced in the dorsal areas.

(Gorfinkiel et al., 1999)

Hemizygotes usually die at the late larval or early pupal stage, with a few animals surviving to the late pharate adult stage. The posterior tergite and posterior hairy zone (PHZ) are deleted in many hemisegments of these pharate adults, and are replaced with a mirror-image duplication of the anterior tergite. The three anterior rows of microchaetae are usually unaffected. A small number of hemitergites are completely missing. Some hemitergites have weaker phenotypes, including deletion of the PHZ, absence of the pigment band, abnormal trichome polarity in the PHZ and posterior tergite, and transformation of macrochaetae into microchaetae. Trichome polarity in the posterior sternite is reversed, and occasionally a few sternal bristles and neighbouring pleural trichomes are affected.

(Kopp and Duncan, 1997)

Dead homozygotes rescued from the pupal case exhibit deletion of the central region of the wing. bi^omb-Gal4/bi^omb-282 flies exhibit deletion of the central wing region. Mitotic clone induced in the wing anterior compartment causes deletion of cells in the distal region, more proximally mutant cells remain. Mitotic clone induced in the wing posterior compartment causes deletion of cells in the distal region, more proximally mutant cells remain.

(Lecuit et al., 1996)

Displays lac and rb phenotypes.

(Pflugfelder et al., 1990)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

bi^omb-282 has visible phenotype, enhanceable by Df(3L)Scf-R11

(Kopp and Duncan, 1997)

Suppressor of

Statement

Reference

bi^omb-282 is a suppressor of visible | dominant phenotype of hh^Mir

(Kopp and Duncan, 1997)

Other

Statement

Reference

Scruffy^R10, bi^omb-282 has visible phenotype

(Kopp and Duncan, 1997)

Phenotype Manifest In

Enhanced by

Statement

Reference

bi^omb-282 has adult abdomen phenotype, enhanceable by Df(3L)Scf-R11

(Kopp and Duncan, 1997)

bi^omb-282 has wing phenotype, enhanceable by Df(3L)Scf-R11

(Kopp and Duncan, 1997)

Suppressor of

Statement

Reference

bi^omb-282 is a suppressor of abdominal tergite phenotype of hh^Mir

(Kopp and Duncan, 1997)

Other

Statement

Reference

Scruffy^R10, bi^omb-282 has abdominal sternite bristle phenotype

(Kopp and Duncan, 1997)

Scruffy^R10, bi^omb-282 has abdominal tergite phenotype

(Kopp and Duncan, 1997)

Additional Comments

Genetic Interactions

Statement

Reference

The tergite and PHZ phenotypes of hh^Mir/+ flies are suppressed by bi^omb-282; in bi^omb-282; hh^Mir double mutants, both the normal and mirror-image duplicated PHZ and posterior tergite structures are missing and replaced with a mirror-image double anterior pattern similar to bi^omb-282 single mutants. However, all segments show double anterior patterning in the double mutants, whereas mirror-image duplications are only seen in a fraction of the segments in bi^omb-282 single mutants. bi^omb-282 does not suppress the formation of ectopic intersegmental membrane seen in hh^Mir/+ flies. The abdomen and wing phenotypes of bi^omb-282 homozygotes are strongly enhanced by Df(3L)Scf-R11. The frequency of complete anterior duplications in the abdominal tergites is strongly increased in bi^omb-282; Scf^R10/Scf^R10 flies compared to bi^omb-282; Scf^R10/+ flies. In addition, the polarity of all sternal bristles is reversed in bi^omb-282; Scf^R10/Scf^R10 flies, whereas only a few, if any, are affected in bi^omb-282 single mutants.

(Kopp and Duncan, 1997)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

Df(1)omb282

bi^omb-282

l(1)bi¹

l(1)omb²⁸²

(Tsai et al., 2015, Sen et al., 2010, Kopp et al., 2000, Kopp and Duncan, 1997, Pflugfelder et al., 1990)

omb²⁸²

(Ruiz-Losada et al., 2021, Simon and Guerrero, 2015, Kopp and Duncan, 2002, Gorfinkiel et al., 1999, Hazelett et al., 1998, Lecuit et al., 1996, Pflugfelder et al., 1992)

Name Synonyms

Secondary FlyBase IDs

FBab0000865

References (12)

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