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General Information
Symbol
Dmel\btlH82Δ3
Species
D. melanogaster
Name
FlyBase ID
FBal0030665
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Ecol\lacZ section of progenitor P{lwB}H82 remains, but 500bp of btl flanking DNA has been deleted. Deleted region includes three high affinity slbo binding sites.
Partial excision of P{lwB}H82, breaking in the w coding region. The deletion removes genomic DNA 3' of the site of insertion towards, but not including, the start site of btl transcription.
Insertion components
P{5'lwB}btlH82Δ3
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Mutant embryos have gaps in the dorsal trunk without ectopic branching. The tracheal cells of heterozygotes show moderate lack-of-migration defects in 68% of embryos, and show severe ectopic lack-of-migration defects in 14% of embryos.
Homozygous clones remain in the proximal region of the dorsal air sac primordium and never colonise the distal region.
Homozygous embryos show little branch migration in the tracheal system. 84% of heterozygous embryos show a tracheal lack of migration phenotype.
btlH82Δ3 homozygous clones in the dorsal air sac primordium grow and divide normally but never contribute to the tip of the primordium.
In stage 14 homozygous btlH82Δ3 embryos there is very little tracheal tubule migration as compared to wild-type. The dorsal trunks of the tracheal system of stage 16 btlH82Δ3 heterozygous embryos lack many dorsal branches.
The failure of tracheal cells to migrate in btlH82Δ3 mutant embryos is accompanied by a failure of these cells to form filopodia.
Homozygous embryos show no tracheal cell migration (although tracheal cells are specified and invaginate properly), resulting in the lack of all tracheal branches.
Homozygous embryos show some inhibition of tracheal branching.
In homozygous stage 15 embryos the tracheal cells fail to migrate, remaining in a tracheal sac-like formation. Expression of btlhs.T:λ\cI-DD in heterozygous btlH82Δ3 embryos causes dorsal trunk defects, 4 or more interruptions.
Clones in which all border cells are btl- show no defects in border cell migration, though mild delays or low frequency defects would not have been detected.
btlLG19/btlH82Δ3 heterozygotes exhibit the tracheal phenotype.
Tracheal pits form, but tracheal cells undergo only limited migration.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
NOT suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
The penetrance of the tracheal lumen defect seen in Vhl1/+ embryos is not modified by btlH82Δ3/+.
The severity and penetrance of the Catsup26/+ tracheal lack of migration phenotype is enhanced by btlH82Δ3/+. The opposing tracheal phenotypes of PurAA17/+ and btlH82Δ3/+ single heterozygous embryos (ectopic migration and lack of migration respectively) are both partially suppressed in PurAA17/+ btlH82Δ3/+ double heterozygous embryos. The opposing tracheal phenotypes of PuZ22/+ and btlH82Δ3/+ single heterozygous embryos (ectopic migration and lack of migration respectively) are both partially suppressed in PuZ22/+ btlH82Δ3/+ double heterozygous embryos.
68.8% of btlH82Δ3/+; awdj2A4/+ transheterozygous embryos have normal tracheal systems, compared to 53.4% in btlH82Δ3/+ embryos. 13.5% of these transheterozygotes have tracheal phenotypes resembling those seen in 46.6% of btlH82Δ3/+ embryos, with the remainder having tracheal phenotypes resembling those due to awdj2A4/+.
jing01094, btlH82Δ3 double heterozygotes show an almost complete loss of trachea. When btlH82Δ3 is also added to trh1, tgo1 double heterozygous 69% of embryos display a loss of about half of their trachea.
Expression of btl::htlBH.Scer\UAS under the control of Scer\GAL4btl.PS at both 18oC and 25oC in btlH82Δ3 embryos results in a complete rescue of tracheal cell migration and correct fusion of the relevant tracheal branches. Terminal tracheal cells are determined at the appropriate positions and in correct numbers. Expression of btl::torScer\UAS.cDa under the control of Scer\GAL4btl.PS at both 18oC and 25oC in btlH82Δ3 embryos results in substantial rescue of tracheal defects. Most tracheal branches develop properly and fusion between adjacent metameres occurs in most segments. Occasionally, dorsal branches are missing and the lateral trunk fails to fuse in between some tracheal metameres. The general pattern of the tracheal system is restored, although the tracheal tree has a "felted" phenotype, with fine branches extending from many positions. Ectopic formation of terminal tracheal cells is seen. Expression of btl::EgfrScer\UAS.cDa under the control of Scer\GAL4btl.PS at both 18oC and 25oC in btlH82Δ3 embryos can rescue all primary branches of the tracheal system, although at reduced frequencies. Many embryos have dorsal and ganglionic branch defects in half or more of the segments. The lateral trunk fuses only sporadically, although the dorsal trunk shows complete fusion in most embryos. The visceral branch is misguided in some embryos. The number of terminal tracheal cells is significantly reduced compared to wild type. Tracheal cells fail to migrate in btlH82Δ3 stumps09904b double mutant embryos. Expression of btlScer\UAS.cDa or btl::htlBH.Scer\UAS under the control of Scer\GAL4btl.PS does not rescue any aspect of tracheal development in btlH82Δ3 stumps09904b double mutant embryos. Expression of btl::torScer\UAS.cDa under the control of Scer\GAL4btl.PS significantly rescues tracheal development in btlH82Δ3 stumps09904b double mutant embryos. Ganglionic branches are rescued almost completely, dorsal branches develop in more than 50% of segments and dorsal trunk fusion occurs efficiently. The lateral trunk does not fuse in these embryos and ectopic terminal cell formation is seen. Expression of btl::EgfrScer\UAS.cDa under the control of Scer\GAL4btl.PS significantly rescues tracheal development in btlH82Δ3 stumps09904b double mutant embryos. The dorsal trunk is disrupted in some embryos, some dorsal branches are missing or misguided and the lateral trunk fails to fuse. Some of the ganglionic branches fail to form and do not migrate in the proper direction.
btlH82Δ3 dominantly enhances the homozygous stumpsYY202 tracheal phenotype. stumpsYY202 dominantly enhances the homozygous btlH82Δ3 tracheal phenotype.
Dominantly enhances the border cell migration defect of slboe7b/slboe14a.
Multiple heat shocks of the constitutively active btl::tort4021b.hs.sev protein can partially rescue tracheal migration in homozygous embryos, a single early heat shock allows reduced correction of the mutant phenotype. Partial rescue can also be achieved by the constitutively active proteins tor13D.hs.sev, Ras85DQ13.hs, phl::tor13D.hs.sev, sev::tor13D.hs.sev, Egfr::tort4021E.hs.sev and htl::tort4021F.hs.sev.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Tracheal cell migration defects can be rescued by btlhs.PM restoring the primary branching pattern. Tracheal cell migration defects of stage 15 embryos cannot be rescued by heat induced expression of btlKR.hs or btlhs.T:λ\cI-DD. Coexpression of btlhs.PM and btlhs.T:λ\cI-DD allows less complete rescue than that provided by btlhs.PM alone.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Phenotype of homozygote less severe than phenotype of heterozygote with Df(3L)fz-GF3b or Df(3L)fz-GS1a. The btl alleles form a phenotypic series with respect to the tracheal phenotype. In decreasing order of severity, btlLG18 = btlLG19 > btlH82Δ3 > btlH82Δ11 > btl6-81Δ1.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (16)