FlyBase Allele Report: Dmel\Cdk1[B47]

When cdc2^B47/cdc2^E1-24 animals are raiseds at a semi-restrictive temperature (26^oC), flies develop until the end of pupariation, but fail to hatch. These animals exhibit a severe loss of bristles. In addition, at 26h APF about 50% of sensory organs are composed of only two cells, lacking shaft and socket cells.

(Fichelson and Gho, 2004)

Lethal phase is during the larval/pupal stage. Homozygous embryos develop essentially normally. RP2 neuron duplication and large RP2 neurons are seen in only 3.6% and 3% of cases respectively. Duplication of muscle DA1 can be seen in embryos carrying four copies of cdc2^A171T (in a cdc2^B47 background) which have been shifted to the non-permissive temperature. RP2 neuron duplication and large RP2 neurons are seen in 42.7% and 4.6% of cases respectively. Temperature upshifts can also result in the arrest of neuroblasts in G2 in these embryos.

(Tio et al., 2001)

Somatic clones in the wing lead to wing cells that are larger than normal which produce either longer than normal wing hairs, or double wing hairs.

(Adler et al., 2000)

cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature show mitotic defects resulting in a reduction in the size of the imaginal discs and a reduction in the cell density in the optic lobe. The nuclei of the abdominal histoblasts are enlarged, and the DNA content is increased to about 16C.

(Hayashi and Yamaguchi, 1999)

cdc2^B47/cdc2^E1-24 females cease to lay eggs when shifted to the restrictive temperature of 29^oC. Dividing cells in the ovary disappear over a period of 5 days after shifting to 29^oC, while nurse cell nuclei persist and increase in size.

(Reed and Orr-Weaver, 1997)

The cdc2^B47/cdc2^E1-24 combination is temperature sensitive. cdc2^B47/cdc2^E1-24 third instar mutants the size of the central nervous system and imaginal discs is severly reduced. Many of the mutants cells in the CNS, salivary gland and imaginal discs are larger and more intensely DAPI-staining than wild type. Cell division is reduced but BrdU incorporation occurs in the salivary gland imaginal ring and CNS during the third instar. Mutations at esg have no further effect on these mutant phenotypes. In cdc2^B47/Df(2L)J27, many cells of the outer part of the optic lobe contain huge bnanded polytene chromsomes comparable in size and morphology to the salivary gland polytene chromosomes. Abdominal histoblasts in cdc2^B47/cdc2^E1-24 larvae accumulate BrdU without increasing cell number, suggesting they are in endocycle.

(Hayashi, 1996)

Males carrying two or four copies of P{Dmcdc2^A171T} at 18^oC or 29^oC exhibit a reduction in testis cell number and increase in size of the nucleus and Nebenkern. Results indicate only one meiotic division occurs in males with two copies of P{Dmcdc2^A171T} and no meiotic divisions in males with four copies. Males carrying two copies still exhibit spermatid differentiation.

(Sigrist et al., 1995)

Larvae contain no imaginal discs. Brain development is abnormal.

(Clegg et al., 1993)

Die after pupariation at the larval-pupal interface. Larvae take 1-2 days longer than wild type to reach the wandering stage. Imaginal discs are missing or rudimentary and larval brains are reduced in size. Endoreplication and polytenization of larval cells is normal, as are salivary gland size and intensity of DNA staining of their polytene nuclei.

(Stern et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

Cdk1^B47 is an enhancer of visible phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk1^B47 is an enhancer of abnormal size phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk1^B47 is an enhancer of visible phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

Suppressor of

Statement

Reference

Cdk1^B47 is a suppressor of increased occurrence of cell division | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Cdk1^B47 is a suppressor of increased cell death | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

cdc2[+]/Cdk1^B47 is a suppressor of increased cell death phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdk1^B47 is a suppressor of visible phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

Other

Statement

Reference

cdc2[+]/Cdk1^B47, CycD¹ has viable phenotype

(Emmerich et al., 2004)

Cdk1^B47, CycD¹ has viable phenotype

(Emmerich et al., 2004)

Cdk1^B47, wts^P8 has viable phenotype

(Tao et al., 1999)

Phenotype Manifest In

Suppressed by

Statement

Reference

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Scer\GAL4^P127/CycA^UAS.cHa/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, suppressible by Scer\GAL4^P127/CycA^UAS.cHa

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has embryonic/larval optic lobe phenotype, suppressible by Xlae\cdc2^UAS.cHa/Scer\GAL4^P127

(Hayashi and Yamaguchi, 1999)

NOT suppressed by

Statement

Reference

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has embryonic/larval optic lobe phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has histoblast & nucleus phenotype, non-suppressible by Scer\GAL4^P127/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Cdk1^E1-24/Cdk1^B47 has imaginal disc phenotype, non-suppressible by Scer\GAL4^P127/CycA^UAS.cHa/Xlae\cdc2^K33R.UAS

(Hayashi and Yamaguchi, 1999)

Enhancer of

Statement

Reference

Cdk1^B47 is an enhancer of eye phenotype of Mmus\Shisa5^UAS.cGa, Scer\GAL4^ey.PH

(Gupta et al., 2008)

Cdk1^B47 is an enhancer of wing phenotype of Scer\GAL4^en-e16E, arm^UAS.cWa

(Greaves et al., 1999)

NOT Enhancer of

Statement

Reference

cdc2[+]/Cdk1^B47 is a non-enhancer of eye | somatic clone phenotype of gang-of-four¹

(Beam and Moberg, 2010)

cdc2[+]/Cdk1^B47 is a non-enhancer of phenotype of png^unspecified

(Lee et al., 2001)

Suppressor of

Statement

Reference

Cdk1^B47 is a suppressor of embryonic/larval salivary gland | embryonic stage phenotype of hkb³⁵¹

(Matthew et al., 2024)

Cdk1[+]/Cdk1^B47 is a suppressor | partially of egg chamber | increased number phenotype of Ras85D^V12.cZa.UASp, Scer\GAL4^{VP16.nanos.UTR}

(Zhang et al., 2024)

cdc2[+]/Cdk1^B47 is a suppressor of eye phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

cdc2[+]/Cdk1^B47 is a suppressor of ommatidium phenotype of shtd¹

(Tanaka-Matakatsu et al., 2007)

Cdk1^B47 is a suppressor of wing phenotype of Scer\GAL4^en-e16E, shg^{i.UAS.Tag:SS(aos),Tag:MYC}

(Greaves et al., 1999)

NOT Suppressor of

Statement

Reference

cdc2[+]/Cdk1^B47 is a non-suppressor of eye | somatic clone phenotype of gang-of-four¹

(Beam and Moberg, 2010)

Other

Statement

Reference

Cdk1^B47, esg⁰⁵⁷²⁹ has imaginal disc phenotype

(Hayashi, 1996)

Cdk1^B47, esg^35Ce-3 has imaginal disc phenotype

(Hayashi, 1996)

Additional Comments

Genetic Interactions

Statement

Reference

The enlarged eye phenotype seen in mosaic animals in which the eye is homozygous for gfr¹ is not modified by cdc2^B47/+.

(Beam and Moberg, 2010)

The defects in the abdominal histoblasts are partially rescued by CycA^Scer\UAS.cHa expressed under the control of Scer\GAL4^P127; the increase in DNA content is limited to 8C.

(Hayashi and Yamaguchi, 1999)

Dominantly suppresses the pupal lethality and overproliferation phenotype of wts^P8 homozygotes. The disc overproliferation phenotype of wts^e26-1 homozygotes is suppressed by one copy of cdc2^B47.

(Tao et al., 1999)

In cdc2^B47/+,esg^35Ce-3/esg⁰⁵⁷²⁹ imaginal discs are reduced in size and sometimes have cells with large nuclei.

(Hayashi, 1996)

Xenogenetic Interactions

Statement

Reference

cdc2^B47 enhances the eye phenotypes seen when Mmus\Shisa5^Scer\UAS.cGa is expressed under the control of Scer\GAL4^dpp.PH.

(Gupta et al., 2008)

The defects of cdc2^B47/cdc2^E1-24 larvae are rescued by Xlae\cdc2^Scer\UAS.cHa expressed under the control of Scer\GAL4^P127, but not by Xlae\cdc2^{K33R.Scer\UAS} expressed under the control of Scer\GAL4^P127. Coexpression of CycA^Scer\UAS.cHa and Xlae\cdc2^{K33R.Scer\UAS} under the control of Scer\GAL4^P127 completely rescues the abdominal histoblast defects seen in cdc2^B47/cdc2^E1-24 larvae at the restrictive temperature. The imaginal disc defects seen in these larvae are not rescued.

(Hayashi and Yamaguchi, 1999)

Complementation and Rescue Data

Fails to complement

Cdk1^GT-000294

(Roote, 2004.11.9)

Rescued by

Cdk1^E1-24/Cdk1^B47 is rescued by Cdk1^{WT.UASp.Venus}/Scer\GAL4^Tub.PU

(Ayeni et al., 2014)

Cdk1^E1-24/Cdk1^B47 is rescued by Cdk1^{T14A.UASp.Venus}/Scer\GAL4^Tub.PU

(Ayeni et al., 2014)

Partially rescued by

Cdk1^B47/Df(2L)J27 is partially rescued by Cdk1^P131S

(Sigrist et al., 1995)

Cdk1^B47/Df(2L)J27 is partially rescued by Cdk1^A171T

(Sigrist et al., 1995)

Cdk1^B47/Df(2L)J27 is partially rescued by Cdk1^G43E

(Sigrist et al., 1995)

Not rescued by

Cdk1^E1-24/Cdk1^B47 is not rescued by Scer\GAL4^Tub.PU/Cdk1^{Y15F.UASp.Venus}

(Ayeni et al., 2014)

Cdk1^E1-24/Cdk1^B47 is not rescued by Scer\GAL4^Tub.PU/Cdk1^{T14A.Y15F.UASp.Venus}

(Ayeni et al., 2014)

Cdk1^B47/Df(2L)J27 is not rescued by Cdk1^G177E

(Sigrist et al., 1995)

Comments

Scer\GAL4^tub.PU-mediated expression of cdc2^{WT.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} rescues the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{T14A.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} rescues the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} does not rescue the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

Scer\GAL4^tub.PU-mediated expression of cdc2^{T14A.Y15F.Scer\UAS.P\T.T:Avic\GFP-YFP.Venus} does not rescue the pupal lethality of cdc2^B47/cdc2^E1-24 animals grown at the restrictive temperature of 25[o]C.

(Ayeni et al., 2014)

Lethality of heterozygotes with Df(2L)J27 can be partially rescued by expression of cdc2^A171T, cdc2^G43E or cdc2^P131S at 18^oC, levels of rescue drop at higher temperatures.

(Sigrist et al., 1995)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

6643

w^*; Cdk1^B47 cn¹ bw¹/CyO, P{ftz-lacB}E3

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (6)

Reported As

Symbol Synonym

Cdc2^B47

(Das et al., 2016)

Cdk1^B47

(Matthew et al., 2024, Li et al., 2021, Emmerich et al., 2004)

Dmcdc2^B47

(Nakajima et al., 2011, Greaves et al., 1999, Larochelle et al., 1998, Reed and Orr-Weaver, 1997, Sigrist et al., 1995)

cdc2^B47

(Ayeni et al., 2014, Beam and Moberg, 2010, Chen et al., 2008, Gupta et al., 2008, Von Stetina et al., 2008, Tanaka-Matakatsu et al., 2007)

cdc2^B7

(Heriche et al., 2003)

cdk1^B47

(Zhang et al., 2024, Huang et al., 2021, Baumgardt et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (30)

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