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General Information
Symbol
Dmel\chp2
Species
D. melanogaster
Name
FlyBase ID
FBal0030754
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The deletion in chp2 starts 3' to exon 6 and removes the remaining coding region. The exact localisation of the second breakpoint could not be determined.

Expression Data
Reporter Expression
Additional Information
Statement
Reference

chp protein is abnormallly distributed in chp mutants. In chp1, levels of protein are reduced along the axons in larvae and pupae. In chp2, little or no protein is detected in third instar larval eye disc, in pupa, or in adult retina.

Normally chp RNA is expressed uniformly throughout photoreceptor cell soma. In chp1 and chp2 mutants, expression is graded and decreases with distance from the nucleus.

 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

In mosaic pupal retinas, chp2 homozygous photoreceptor cells the stalk and rhabdomeres are properly separated and the microvillar necks are properly developed similar to controls.

Homozygous chp2 is viable and show reduction and disorganization of the apical microvilli of rhabdomeres in the adult eye.

chp2 mutant photoreceptor cells do not undergo light-dependent degeneration. Despite a nearly complete loss of microvilli, no sign of apoptosis is detected. The phenotype is unaltered under light stress, with or without dietary vitamin A.

Rhabdomere microvilli in chp2 mutants are relatively short, have variable length and are separated from each other.

Abnormal rhabdomeric membranes.

Rudimentary rhabdomeres.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

chp[+]/chp2, prom1 has rhabdomere phenotype, enhanceable by eys[+]/eys1

chp[+]/chp2, prom1 has ommatidium phenotype, enhanceable by eys[+]/eys1

chp2, eyc[+]/prom1 has rhabdomere phenotype, enhanceable by eys[+]/eys1

chp2, eyc[+]/prom1 has ommatidium phenotype, enhanceable by eys[+]/eys1

Enhancer of
Suppressor of
Statement
Reference

chp[+]/chp2 is a suppressor | partially of rhabdomere phenotype of prom1

chp[+]/chp2 is a suppressor | partially of ommatidium phenotype of prom1

Other
Additional Comments
Genetic Interactions
Statement
Reference

In mosaic retinas, chp2 and Synd661T double homozygous photoreceptor cells during pupal stages the stalk-rhabdomere separation is severely and prominently disrupted (even before catacomb-like membrane architecture formation); in adults microvilli show no co-adherence instead spread across the entire apical membrane adult mosaic retina when compared to controls.

Trans-heterozygous crb11A22/+ chp2 flies exhibit rhabdomeres with significantly smaller width than wild-type. The length of the rhabdomeres is not affected.

prom1/prom1; chp2/+ double mutant flies show suppression of the prom1/prom1 rhabdomeral phenotype. However, in prom1/prom1, eys1/+; chp2/+ flies the rhabdomeres are stuck together, so the mild prom1/prom1; chp2/+ phenotype is enhanced in prom1/prom1, eys1/+; chp2/+ flies.

In prom1/prom1; chp2/+ double mutant flies, the proportion of flies with one aberrant fused cluster of rhabdomeres per ommatidium is smaller than in prom1/prom1 single mutants. In the double mutant flies, some ommatidia show the wild-type pattern of 7 individual rhabdomeres, while most ommatidia show between 2 and 5 individual rhabdomeres, and a small proportion show one fused cluster of rhabdomeres. In prom1/prom1, eys1/+; chp2/+ triple mutant flies, the rescue of the prom1/prom1 phenotype is reduced compared to the prom1/prom1; chp2/+ flies, with most ommatidia in the triple mutants showing between 0 and 3 individual rhabdomeres and no ommatidia showing more than 3 individual rhabdomeres.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (10)