FB2025_01 , released February 20, 2025
Allele: Dmel\abclu-1
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General Information
Symbol
Dmel\abclu-1
Species
D. melanogaster
Name
FlyBase ID
FBal0030791
Feature type
allele
Associated gene
Dmel\ab
Associated Insertion(s)
Carried in Construct
Also Known As
abclu1, clu1
Key Links
Genomic Maps

Allele class
Mutagen
Nature of the Allele
Allele class
Mutagen
ethyl methanesulfonate
(van Vactor et al., 1993)
Progenitor genotype
Cytology
Description

Amino acid replacement: R531term.

(Hu et al., 1995)

Mutation results in premature termination before the ZF DNA-binding domain.

(Hu et al., 1995)

Nucleotide substitution: C?T.

(Hu et al., 1995)
Mutations Mapped to the Genome
Curation Data
Type
Location
Additional Notes
References
point_mutation
2L:11,258,220..11,258,220 [+]
Nucleotide change:

C11258220T

Amino acid change:

R531term | ab-PA; R521term | ab-PB; R521term | ab-PD; R521term | ab-PF

Comment:

The amino acid position of the mutation is reported with respect to ab-PA.

(Hu et al., 1995)
Variant Molecular Consequences
Associated Sequence Data
DDBJ / EMBL / GenBank
DNA sequence
Protein sequence
 
UniProtKB/Swiss-Prot
    UniProtKB/TrEMBL
      Expression Data
      Reporter Expression
      Additional Information
      Statement
      Reference
       
      Marker for
      Reflects expression of
      Reporter construct used in assay
      Human Disease Associations
      Disease Ontology (DO) Annotations
      Models Based on Experimental Evidence ( 0 )
      Disease
      Evidence
      References
      Modifiers Based on Experimental Evidence ( 0 )
      Disease
      Interaction
      References
      Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
       
      Disease-implicated variant(s)
       
      Phenotypic Data
      Phenotypic Class
      Phenotype Manifest In

      abdominal posterior ventral multidendritic neuron vdap

      (Sugimura et al., 2004)

      abdominal posterior ventral multidendritic neuron vdap (with abk02807)

      (Sugimura et al., 2004)

      larval abdominal segmental nerve

      (Hu et al., 1995, van Vactor et al., 1993)

      larval dorsal multidendritic neuron ddaD | somatic clone

      (Sugimura et al., 2004)

      larval dorsal multidendritic neuron ddaE

      (Sugimura et al., 2004)

      larval dorsal multidendritic neuron ddaE (with abk02807)

      (Sugimura et al., 2004)

      larval dorsal multidendritic neuron ddaE | somatic clone

      (Sugimura et al., 2004)

      larval transverse nerve

      (Hu et al., 1995, van Vactor et al., 1993)

      muscle cell of abdominal 2 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 2 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 2 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 2 lateral oblique muscle 1

      (Hu et al., 1995)

      muscle cell of abdominal 3 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 3 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 3 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 3 lateral oblique muscle 1

      (Hu et al., 1995)

      muscle cell of abdominal 4 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 4 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 4 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 4 lateral oblique muscle 1

      (Hu et al., 1995)

      muscle cell of abdominal 5 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 5 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 5 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 5 lateral oblique muscle 1

      (Hu et al., 1995)

      muscle cell of abdominal 6 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 6 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 6 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 6 lateral oblique muscle 1

      (Hu et al., 1995)

      muscle cell of abdominal 7 dorsal acute muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 7 dorsal oblique muscle 3

      (Hu et al., 1995)

      muscle cell of abdominal 7 dorsal oblique muscle 5

      (Hu et al., 1995)

      muscle cell of abdominal 7 lateral oblique muscle 1

      (Hu et al., 1995)

      wing

      (Caygill and Johnston, 2008)

      wing vein L5 (with ab1)

      (Hu et al., 1995)
      Detailed Description
      Statement
      Reference

      abclu-1/abk02807 transheterozygotes die during early larval stages.

      (Hattori et al., 2013)

      Heterozygotes show an increase in wing size compared to controls.

      (Caygill and Johnston, 2008)

      abclu-1 homozygotes and abclu-1/abk02807 transheterozygotes hatch and grow normally until 30 hours AEL. The ddaE neurons of abclu-1/abk02807 and abclu-1 larvae do not display such a comb-like design of dendritic arbors as wild-type ddaE neurons. Instead, mutant neurons produce supernumerary terminals that do not necessarily show oriented growth. About 20% of mutant ddaE cells do not extend lateral primary branches and in extreme cases, a single mutant ddaE extends two primary branches dorsally instead. Time-lapse recording of mutant ddaE shows that secondary branches appear with curved morphology and remain associated with fine higher-order branches that are oriented in dorsal and ventral directions. Ventral class I ventral posterior da (vpda) neurons also show dendritic pattern defects.

      ddaD and ddaE abclu-1 mutant neuron clones in heterozygous mature larvae take on an abnormal shape abd produce branch terminals in excess. The ddaD neuron shows a statistically significant increase in the dendritic arbor area size while the area size of ddaE neurons is not significantly different. Class II, III and IV neuron clones do not show dendritic pattern defects.

      (Sugimura et al., 2004)

      During embryogenesis the SNb axons pause at the edge of muscle 13 and form abnormal branches, instead of forming their wild type axonal extensions onto the muscle fibers. These aberrant branches wander over the prospective target muscles and occasionally form connections at ectopic sites. Mutants do not establish the three branched terminal arbor on muscles 7, 6, 13 and 12. The transverse nerve is often incomplete and invades the ventral muscle field. A few muscles (3, 5, 11, 20) show variably penetrant defects in their attachment to the epidermis, and variable defects in the location of their attachments. The muscle detachment phenotype of other strong alleles is not evident in abclu-1. ab1/abclu-1 heterozygotes show wing vein L5 failing to reach the margin of the wing.

      (Hu et al., 1995)

      Segmental nerve b fails to make some or all of its stereotyped connections in most segments. CNS development is normal. Ectopic arborization over the ventral muscles from the transverse nerve is often observed: possibly a secondary effect of the failure of segmental nerve b axons to synapse onto their correct targets.

      (van Vactor et al., 1993)
      External Data
      Interactions
      Show genetic interaction network for Enhancers & Suppressors
      Phenotypic Class
      Suppressor of
      Statement
      Reference

      ab1/abclu-1 is a suppressor | partially of some die during P-stage phenotype of mir-125KO.C, mir-let7KO.C

      (Caygill and Johnston, 2008)
      Phenotype Manifest In
      Additional Comments
      Genetic Interactions
      Statement
      Reference
      Xenogenetic Interactions
      Statement
      Reference
      Complementation and Rescue Data
      Images (0)
      Mutant
      Wild-type
      Stocks (1)
      Notes on Origin
      Discoverer
      External Crossreferences and Linkouts ( 0 )
      Synonyms and Secondary IDs (3)
      References (9)