Allele Dmel\dpp^hr92

General Information
Symbol	Dmel\dpp^hr92	Species	D. melanogaster
Name		FlyBase ID	FBal0031071
Feature type	allele	Associated gene	Dmel\dpp

Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Arora and Nusslein-Volhard, 1992)
Mutagen	ethyl methanesulfonate (Wharton et al., 1993, Wharton et al., 1996)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: G2947A. Amino acid replacement: C587Y. Amino acid 587 falls in the C-terminal ligand region of the dpp protein. (Wharton et al., 1996)
Cytology
Phenotypic Data
Phenotypic Class
	lethal (Wharton et al., 1993) lethal \| embryonic stage (Wharton et al., 1996) visible \| adult stage \| dominant (Dahal et al., 2012)
Phenotype Manifest In
	abdominal segment (Wharton et al., 1996) amnioserosa (Arora and Nusslein-Volhard, 1992) antennal sense organ (Arora and Nusslein-Volhard, 1992) cephalopharyngeal skeleton (Wharton et al., 1996) denticle belt & thorax (Wharton et al., 1996) dorsal hair \| embryonic stage (Arora and Nusslein-Volhard, 1992) embryonic anal pad (Takashima and Murakami, 2001) embryonic dorsal epidermis (Wharton et al., 1996) embryonic epidermis \| dorsal (Arora and Nusslein-Volhard, 1992) embryonic hindgut (Takashima and Murakami, 2001) embryonic large intestine (Takashima and Murakami, 2001) embryonic thorax (Wharton et al., 1996) filzkorper (Wharton et al., 1996) maxillary palp sense organ (Arora and Nusslein-Volhard, 1992) wing (Dahal et al., 2012)
Detailed Description
	Statement Reference dpp[hr92]/+ mutant wings show thickened, bifurcated veins. (Dahal et al., 2012) The hindgut of heterozygous and homozygous embryos is significantly shorter than that of wild-type embryos. In heterozygotes, the hindgut is associated with abnormally internalised anal pads. In homozygous embryos, the small intestine and rectum form normally, but the large intestine is very short. (Takashima and Murakami, 2001) Embryos show cuticular holes, disorganization of the head skeleton, internalization of the seventh and eighth abdominal segments and a thoracic constriction. The first and second ventral denticle belts are expanded up to the dorsal midline. The filzkorper are entirely absent. (Wharton et al., 1996) Survival of heterozygotes is reduced if also heterozygous for certain alleles of tld. (Finelli et al., 1994) Dominant lethality less than 50%. Homozygous and transheterozygous embryos were examined with respect to 25 cuticular markers, results demonstrate a graded requirement for dpp along the dorso-ventral axis. (Wharton et al., 1993) Moderate ventralised phenotype. Rings of ventral denticle belts differentiate around the entire dorsoventral axis, almost no dorsal hairs are seen and the antennal and maxillary sense organs are missing. Defective movements of the germ band: due to loss of the amnioserosa and because the dorsalmost cells have acquired the lateral fate of the dorsal ectoderm. Dorsal cell fates are deleted and ventrolateral mitotic domains are expanded. (Arora and Nusslein-Volhard, 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference dpp^hr92 has lethal \| recessive \| embryonic stage phenotype, enhanceable \| maternal effect by Df(2L)C144/+ (Su et al., 2001) dpp^hr92 has lethal \| recessive \| embryonic stage phenotype, enhanceable \| maternal effect by Df(2L)DTD52-D51/+ (Su et al., 2001) dpp^hr92 has lethal \| recessive \| embryonic stage phenotype, enhanceable \| maternal effect by lilli^a16/lilli[+] (Su et al., 2001) dpp^hr92 has lethal \| recessive \| embryonic stage phenotype, enhanceable \| maternal effect by T(2;3)DTD46.4/+ (Su et al., 2001)
Enhancer of
Statement Reference dpp[+]/dpp^hr92 is an enhancer of visible \| adult stage phenotype of Irk2^DN.Scer\UAS, Scer\GAL4^Bx-MS1096 (Dahal et al., 2012)
NOT Enhancer of
Statement Reference dpp[+]/dpp^hr92 is a non-enhancer of visible \| adult stage phenotype of Irk2^Scer\UAS.cDa, Scer\GAL4^da.G32 (Dahal et al., 2012)
Phenotype Manifest In
Enhanced by
Statement Reference dpp^hr92 has phenotype, enhanceable by tld¹⁴ (Raftery et al., 1995)
Enhancer of
Statement Reference dpp[+]/dpp^hr92 is an enhancer of wing phenotype of Irk2^DN.Scer\UAS, Scer\GAL4^Bx-MS1096 (Dahal et al., 2012)
NOT Enhancer of
Statement Reference dpp[+]/dpp^hr92 is a non-enhancer of wing vein phenotype of Irk2^Scer\UAS.cDa, Scer\GAL4^Bx-MS1096 (Dahal et al., 2012)
Suppressor of
Statement Reference dpp^hr92 is a suppressor \| partially of female germline stem cell \| supernumerary phenotype of Su(var)3-3^ΔN (Eliazer et al., 2011)
Other
Statement Reference Ack⁸⁶, dpp[+]/dpp^hr92 has embryo \| dorsal closure stage phenotype (Zahedi et al., 2008)
Additional Comments
Genetic Interactions
Statement Reference One copy of dpp[hr92] enhances the wing defects seen when Irk2[DN.Scer\UAS] is expressed under the control of Scer\GAL4[Bx-MS1096]. 84% of wings from female flies expressing the P{UAS-Irk2.DN}5.2 insertion line display defects, with 29% of these considered to be severe. When the stronger P{UAS-Irk2.DN}5.1 insertion line is expressed 97% of wings from female flies display severe defects. One copy of dpp[hr92] does not enhance the wing vein defects seen when Irk2[Scer\UAS.cDa] is expressed under the control of Scer\GAL4[Bx-MS1096]. (Dahal et al., 2012) dpp[hr92]/+ partially suppresses the Su(var)3-3[ΔN] mutant supernumerary germline cell phenotype resulting in an increased number of germline cysts with branched fusomes and maturing egg chambers. (Eliazer et al., 2011) dpp[hr92]/+ ; Ack[86]/Ack[86] embryos show dorsal closure defects at a relatively low frequency, but which is significantly higher than the frequency seen in either dpp[hr92]/+ or Ack[86]/Ack[86] single mutant embryos. (Zahedi et al., 2008)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2 )
Bloomington	2069 dpp^hr92 cn¹ bw¹/SM6a
Kyoto	106649 dpp^hr92 cn¹ bw¹/SM6a
Notes on Origin
Discoverer	Blackman.

Comments
	Based on considerations of degree of dominant lethality, dpp alleles can be placed in an allelic series. Progressing from weakest to most severe the series is: dpp^e87 < dpp^e90 < dpp^hr56 < dpp^hr4 < dpp^hr92 < dpp^hr27 < dpp^hr93 < dpp^H94 < dpp^H95 < dpp^H96 = dpp^H91 = dpp^H46. (Wharton et al., 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 3 )
Reported As
Symbol Synonym	dpp⁹² (Iwaki and Lengyel, 2002) dpp^hr92 (Scuderi et al., 2006, Bangi and Wharton, 2006, Zahedi et al., 2008, Pilgram et al., 2011, Eliazer et al., 2011, Dahal et al., 2012) hin-r92 (Arora and Nusslein-Volhard, 1992)
Name Synonym
Secondary FlyBase IDs

References ( 18 )
Research paper	Dahal et al., 2012, Development 139(19): 3653--3664 An inwardly rectifying K+ channel is required for patterning. [FBrf0219361] Eliazer et al., 2011, Proc. Natl. Acad. Sci. U.S.A. 108(17): 7064--7069 Loss of lysine-specific demethylase 1 nonautonomously causes stem cell tumors in the Drosophila ovary. [FBrf0213593] Pilgram et al., 2011, J. Neurosci. 31(2): 492--500 The RhoGAP crossveinless-c Interacts with Dystrophin and Is Required for Synaptic Homeostasis at the Drosophila Neuromuscular Junction. [FBrf0212767] Zahedi et al., 2008, Dev. Dyn. 237(10): 2936--2946 Leading edge-secreted Dpp cooperates with ACK-dependent signaling from the amnioserosa to regulate myosin levels during dorsal closure. [FBrf0205999] Bangi and Wharton, 2006, Dev. Biol. 295(1): 178--193 Dpp and Gbb exhibit different effective ranges in the establishment of the BMP activity gradient critical for Drosophila wing patterning. [FBrf0192202] Scuderi et al., 2006, Dev. Biol. 291(1): 110--122 scylla and charybde, homologues of the human apoptotic gene RTP801, are required for head involution in Drosophila. [FBrf0190159] Chen et al., 2005, Dev. Biol. 281(2): 270--285 Allocation and specification of the genital disc precursor cells in Drosophila. [FBrf0187379] Iwaki and Lengyel, 2002, Mech. Dev. 114(1-2): 71--84 A Delta-Notch signaling border regulated by Engrailed/Invected repression specifies boundary cells in the Drosophila hindgut. [FBrf0147142] Stronach and Perrimon, 2001, Development 128(15): 2905--2913 Investigation of leading edge formation at the interface of amnioserosa and dorsal ectoderm in the Drosophila embryo. [FBrf0138365] Su et al., 2001, Genetics 157(2): 717--725 A screen for modifiers of decapentaplegic mutant phenotypes identifies lilliputian, the only member of the Fragile-X/Burkitt's Lymphoma family of transcription factors in Drosophila melanogaster. [FBrf0132343] Takashima and Murakami, 2001, Mech. Dev. 101(1-2): 79--90 Regulation of pattern formation in the Drosophila hindgut by wg, hh, dpp, and en. [FBrf0134749] Gajewski et al., 1998, Dev. Genes Evol. 208(7): 382--392 Combinatorial control of Drosophila mef2 gene expression in cardiac and somatic muscle cell lineages. [FBrf0104705] Wharton et al., 1996, Genetics 142(2): 493--505 Molecular lesions associated with alleles of decapentaplegic identify residues necessary for TGF- BMP cell signaling in Drosophila melanogaster. [FBrf0086682] Raftery et al., 1995, Genetics 139(1): 241--254 Genetic screens to identify elements of the decapentaplegic signaling pathway in Drosophila. [FBrf0079237] Childs and O'Connor, 1994, Dev. Biol. 162(1): 209--220 Two domains of the tolloid protein contribute to its unusual genetic interaction with decapentaplegic. [FBrf0068447] Finelli et al., 1994, Development 120(4): 861--870 Mutational analysis of the Drosophila tolloid gene, a human BMP-1 homolog. [FBrf0073067] Wharton et al., 1993, Development 117(2): 807--822 An activity gradient of decapentaplegic is necessary for the specification of dorsal pattern elements in the Drosophila embryo. [FBrf0058100] Arora and Nusslein-Volhard, 1992, Development 114: 1003--1024 Altered mitotic domains reveal fate map changes in Drosophila embryos mutant for zygotic dorsoventral patterning genes. [FBrf0055835]

Allele Dmel\dpphr92

Allele Dmel\dpp^hr92