Allele Dmel\drk^e0A

General Information
Symbol	Dmel\drk^e0A	Species	D. melanogaster
Name		FlyBase ID	FBal0031081
Feature type	allele	Associated gene	Dmel\drk
Also Known As	drk^eOA

Allele class	antimorphic allele - genetic evidence
Mutagen
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	antimorphic allele - genetic evidence (Cooper et al., 1996)
Mutagen
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Amino acid replacement: H106Y. (Moressis et al., 2009)
Cytology
Phenotypic Data
Phenotypic Class
	learning defective (Moressis et al., 2009) lethal \| recessive (Simon et al., 1991, Cooper et al., 1996) visible \| dominant (Cooper et al., 1996)
Phenotype Manifest In
	photoreceptor cell R7 (Cooper et al., 1996)
Detailed Description
	Statement Reference Olfactory association learning is lower in drk[e0A] heterozygotes compared to controls. Histological and immunohistochemical examination of drk[e0A] heterozygous heads with multiple antigenic markers does not reveal and gross structural anomalies in the brain. Avoidance of odors used as CS and electroshock (US) are no different from that of controls. Heterozygous mutants exhibit normal odor avoidance after pre-exposure to electric shock. (Moressis et al., 2009) Mutation disrupts development of receptor cell R7. (Cooper et al., 1996) Reduces the ability of a cell to develop as an R7 cell or as any other photoreceptor. (Simon et al., 1991)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement Reference drk^e0A has learning defective phenotype, suppressible by Scer\GAL4^c747/phl^fl.Scer\UAS (Moressis et al., 2009) drk^e0A has learning defective phenotype, suppressible by Scer\GAL4^c747/phl^{Scer\UAS.F179} (Moressis et al., 2009)
Enhancer of
Statement Reference drk[+]/drk^e0A is an enhancer of size defective \| adult stage phenotype of sl² (Murillo-Maldonado et al., 2011)
NOT Enhancer of
Statement Reference drk[+]/drk^e0A is a non-enhancer of size defective \| adult stage phenotype of sl⁹ (Murillo-Maldonado et al., 2011) drk^e0A is a non-enhancer of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-enhancer of visible phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-enhancer of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005)
Suppressor of
Statement Reference drk[+]/drk^e0A is a suppressor \| partially of increased cell number \| adult stage phenotype of sl² (Murillo-Maldonado et al., 2011) drk[+]/drk^e0A is a suppressor of visible phenotype of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^69B (MacDougall et al., 2004) drk[+]/drk^e0A is a suppressor of visible phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006) drk[+]/drk^e0A is a suppressor of visible phenotype of Src64B^Δ540.hs.sev (Cooper et al., 1996)
NOT Suppressor of
Statement Reference drk^e0A is a non-suppressor of visible phenotype of C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3}, Scer\GAL4^dpp.blk1 (Ishimaru et al., 1999) drk^e0A is a non-suppressor of visible phenotype of C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ishimaru et al., 1999) drk^e0A is a non-suppressor of visible phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-suppressor of visible phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-suppressor of visible phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005)
Phenotype Manifest In
Enhancer of
Statement Reference drk[+]/drk^e0A is an enhancer of wing blade phenotype of sl² (Murillo-Maldonado et al., 2011) drk^e0A is an enhancer of longitudinal connective phenotype of Khc::Ggal\MLCK^KA.ftz (Fritz and VanBerkum, 2000) drk^e0A is an enhancer of phenotype of svp^hs.ro1 (Kramer et al., 1995)
NOT Enhancer of
Statement Reference drk[+]/drk^e0A is a non-enhancer of wing blade phenotype of sl⁹ (Murillo-Maldonado et al., 2011) drk^e0A is a non-enhancer of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-enhancer of eye phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-enhancer of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005) Scer\GAL4^tub.PU/drk^e0A is a non-enhancer of wing vein phenotype of Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} (Oishi et al., 2009)
Suppressor of
Statement Reference drk[+]/drk^e0A is a suppressor \| partially of photoreceptor cell R7 \| adult stage phenotype of sl² (Murillo-Maldonado et al., 2011) drk[+]/drk^e0A is a suppressor \| partially of wing vein \| ectopic phenotype of sl² (Murillo-Maldonado et al., 2011) drk[+]/drk^e0A is a suppressor of dorsal appendage phenotype of Cbl^L.Scer\UAS, Scer\GAL4^EQ1 (Wang and Pai, 2011) drk[+]/drk^e0A is a suppressor of dorsal appendage phenotype of Cbl^{L.Δ70Z.Scer\UAS}, Scer\GAL4^EQ1 (Wang and Pai, 2011) drk[+]/drk^e0A is a suppressor of eye phenotype of Src64B^Δ540.hs.sev (Cooper et al., 1996) drk[+]/drk^e0A is a suppressor of wing vein \| ectopic phenotype of Pi3K68D^{KD.Scer\UAS.T:Hsap\MYC}, Scer\GAL4^69B (MacDougall et al., 2004) drk[+]/drk^e0A is a suppressor of wing vein \| ectopic phenotype of Scer\GAL4^tub.PU, csw^{N308D.Scer\UAS.P\T} (Oishi et al., 2006) drk^e0A is a suppressor of phenotype of Src42A^KR.hs.2sev (Takahashi et al., 1996) drk^e0A is a suppressor of phenotype of svp^2.sev (Kramer et al., 1995) drk^e0A is a suppressor of photoreceptor cell R1 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a suppressor of photoreceptor cell R2 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a suppressor of photoreceptor cell R3 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a suppressor of photoreceptor cell R4 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a suppressor of photoreceptor cell R5 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a suppressor of photoreceptor cell R6 \| ectopic phenotype of B-H1^sev.PH (Hayashi et al., 1998)
NOT Suppressor of
Statement Reference drk[+]/drk^e0A is a non-suppressor of dorsal appendage phenotype of Cbl^{L.mPR.Scer\UAS}, Scer\GAL4^EQ1 (Wang and Pai, 2011) drk^e0A is a non-suppressor of eye phenotype of C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3}, Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Ishimaru et al., 1999) drk^e0A is a non-suppressor of eye phenotype of CanB^GMR.PS, Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-suppressor of eye phenotype of Pp2B-14D^act.GMR (Sullivan and Rubin, 2002) drk^e0A is a non-suppressor of eye phenotype of Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF, btl^{Scer\UAS.T:λ\cI-DD}, stumps^{Scer\UAS.T:Zzzz\FLAG} (Zhu et al., 2005) drk^e0A is a non-suppressor of photoreceptor cell R7 phenotype of B-H1^sev.PH (Hayashi et al., 1998) drk^e0A is a non-suppressor of wing vein L3 phenotype of C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3}, Scer\GAL4^dpp.blk1 (Ishimaru et al., 1999) Scer\GAL4^tub.PU/drk^e0A is a non-suppressor of wing vein phenotype of Scer\GAL4^tub.PU, csw^{Y279C.Scer\UAS.P\T} (Oishi et al., 2009)
Other
Statement Reference da², drk[+]/drk^e0A has follicle cell phenotype (Smith et al., 2002) da², drk^e0A has follicle cell phenotype (Smith et al., 2002)
Additional Comments
Genetic Interactions
Statement Reference One copy of drk[e0A] enhances the reduction in wing blade area seen in homozygous sl[2] males. One copy of drk[e0A] does not enhance the reduction in wing blade area seen in homozygous sl[9] males. One copy of drk[e0A] partially suppresses the ectopic wing vein phenotype seen in sl[2] homozygotes. One copy of drk[e0A] partially suppresses the percentage of sl[2] mutant ommatidia that contain extra R7 photoreceptors. (Murillo-Maldonado et al., 2011) drk[e0A]/+ significantly suppresses the ventralised egg shell phenotype observed when Cbl[L.Scer\UAS] is expressed in follicle cells under the control of Scer\GAL4[EQ1]. drk[e0A]/+ fails to suppresses the ventralised egg shell phenotype observed when Cbl[L.mPR.Scer\UAS] is expressed in follicle cells under the control of Scer\GAL4[EQ1]. The dorsalised eggshell phenotype observed when Cbl[L.Δ70Z.Scer\UAS] is expressed under the control of Scer\GAL4[EQ1] is suppressed in a drk[e0A]/+ background. (Wang and Pai, 2011) Expression of phl[fl.Scer\UAS] under the control of Scer\GAL4[c747] results in full reversal of the learning deficit of drk[e0A] heterozygotes. Expression of phl[Scer\UAS.F179] under the control of Scer\GAL4[c747] results in full reversal of the learning deficit of drk[e0A] heterozygotes. (Moressis et al., 2009) The presence of a drk[e0A] background does not affect ectopic wing vein formation in csw[Y279C.Scer\UAS] (Scer\GAL4[tub]) mutants. (Oishi et al., 2009) drk[e0A]/+ suppresses the ectopic wing vein phenotype caused by expression of Pi3K68D[KD.Scer\UAS.T:Hsap\MYC] under the control of Scer\GAL4[69B]. (MacDougall et al., 2004) Weakly enhances the eye phenotype produced by activated arm constructs. (either arm^S44Y.GMR or arm^S56F.GMR). (Freeman and Bienz, 2001) Has no effect on the eye phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^GMR.PF. Has no effect on the wing vein phenotype caused by C3G^{Scer\UAS.T:Hsap\MYC,T:Ggal\Myr3} expressed under the control of Scer\GAL4^dpp.blk1. (Ishimaru et al., 1999) drk^e0A significantly suppresses the formation of extra outer photoreceptors caused by B-H1^sev.PH, although it does not affect the loss of R7 cell phenotype caused by B-H1^sev.PH. (Hayashi et al., 1998) Presence of the mutation in Src64B^Δ540.hs.sev flies alleviates the rough eye phenotype. (Cooper et al., 1996) No effect on the faf eye phenotype. (Huang and Fischer-Vize, 1996) Mutation has no effect on the rough eye phenotype caused by two insertions of P{GMR-Rho1}. (Hariharan et al., 1995) Suppression of the rough eye phenotype (formation of extra R7 cells) caused by P{sev-svp2} and enhancement of the P{ro-svp1} rough eye phenotype (loss of one or more outer photoreceptors from many of the ommatidia). (Kramer et al., 1995) Enhances the sev^B4 phenotype: disrupts sev^B4 protein signalling. (Simon et al., 1991)
Xenogenetic Interactions
Statement Reference The penetrance of the Fas2-positive axon crossover phenotype seen in embryos carrying Khc::Ggal\MLCK^KA.ftz is increased if they are also homozygous for drk^e0A. (Fritz and VanBerkum, 2000)
Complementation & Rescue Data
Rescued by	drk^e0A is rescued by drk^hs.PM (Moressis et al., 2009)
Comments
Stocks ( 2 )
Bloomington	5691 sev¹⁴; drk^e0A/CyO
Kyoto	108368 sev¹⁴; drk^e0A/CyO
Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 8 )
Reported As
Symbol Synonym	drk^E0A (Huang and Fischer-Vize, 1996, Moressis et al., 2009) drk^e0A (Oishi et al., 2009, MacDougall et al., 2004, Murillo-Maldonado et al., 2011, Oishi et al., 2006) drk^eOA (Smith et al., 2002) drk^eoa (Fritz and VanBerkum, 2000) drk^eOa (Schweitzer et al., 1995) Drk^EOA (Wang and Pai, 2011) Drk^H106Y (Cooper et al., 1996) E(sev)2B^e0A (Takahashi et al., 1996, )
Name Synonym
Secondary FlyBase IDs

References ( 20 )
Research paper	Murillo-Maldonado et al., 2011, PLoS ONE 6(11): e28067 Insulin Receptor-Mediated Signaling via Phospholipase C-γ Regulates Growth and Differentiation in Drosophila. [FBrf0216849] Wang and Pai, 2011, PLoS ONE 6(2): e17097 D-Cbl Binding to Drk Leads to Dose-Dependent Down-Regulation of EGFR Signaling and Increases Receptor-Ligand Endocytosis. [FBrf0213073] Moressis et al., 2009, J. Neurosci. 29(8): 2611--2625 A dual role for the adaptor protein DRK in Drosophila olfactory learning and memory. [FBrf0207513] Oishi et al., 2009, Hum. Mol. Genet. 18(1): 193--201 Phosphatase-defective LEOPARD syndrome mutations in PTPN11 gene have gain-of-function effects during Drosophila development. [FBrf0207302] Oishi et al., 2006, Hum. Mol. Genet. 15(4): 543--553 Transgenic Drosophila models of Noonan syndrome causing PTPN11 gain-of-function mutations. [FBrf0190828] Zhu et al., 2005, Genetics 170(2): 767--777 A screen for genes that influence fibroblast growth factor signal transduction in Drosophila. [FBrf0187664] MacDougall et al., 2004, Mol. Cell. Biol. 24(2): 796--808 Targeted expression of the class II phosphoinositide 3-kinase in Drosophila melanogaster reveals lipid kinase-dependent effects on patterning and interactions with receptor signaling pathways. [FBrf0167897] Smith et al., 2002, Development 129(13): 3255--3267 daughterless coordinates somatic cell proliferation, differentiation and germline cyst survival during follicle formation in Drosophila. [FBrf0148972] Sullivan and Rubin, 2002, Genetics 161(1): 183--193 The Ca(2+)-calmodulin-activated protein phosphatase calcineurin negatively regulates EGF receptor signaling in Drosophila development. [FBrf0149012] Freeman and Bienz, 2001, EMBO Rep. 2(2): 157--162 EGF receptor/Rolled MAP kinase signalling protects cells against activated Armadillo in the Drosophila eye. [FBrf0134599] Fritz and VanBerkum, 2000, Development 127(9): 1991--2000 Calmodulin and Son of sevenless dependent signaling pathways regulate midline crossing of axons in the Drosophila CNS. [FBrf0127081] Ishimaru et al., 1999, EMBO J. 18(1): 145--155 Activation of the Drosophila C3G leads to cell fate changes and overproliferation during development, mediated by the RAS-MAPK pathway and RAP1. [FBrf0106038] Hayashi et al., 1998, Dev. Biol. 200(2): 131--145 Specification of primary pigment cell and outer photoreceptor fates by BarH1 homeobox gene in the developing Drosophila Eye. [FBrf0104448] Cooper et al., 1996, Cell Growth Diffn 7(11): 1435--1441 Signaling by ectopically expressed Drosophila Src64 requires the protein-tyrosine phosphatase Corkscrew and the adapter Downstream of receptor kinases. [FBrf0091036] Huang and Fischer-Vize, 1996, Development 122(10): 3207--3216 Undifferentiated cells in the developing Drosophila eye influence facet assembly and require the Fat facets ubiquitin-specific protease. [FBrf0090618] Takahashi et al., 1996, Genes Dev. 10(13): 1645--1656 Regulation of cell-cell contacts in developing Drosophila eyes by Dsrc41, a new, close relative of vertebrate c-src. [FBrf0089086] Hariharan et al., 1995, EMBO J. 14(2): 292--302 Characterization of rho GTPase family homologues in Drosophila melanogaster: Overexpressing Rho1 in retinal cells causes a late developmental defect. [FBrf0080078] Kramer et al., 1995, Development 121(5): 1361--1372 Cell fate control in the Drosophila retina by the orphan receptor seven-up: its role in the decisions mediated by the ras signaling pathway. [FBrf0078827] Schweitzer et al., 1995, Nature 376(6542): 699--702 Inhibition of Drosophila EGF receptor activation by the secreted protein Argos. [FBrf0083425] Simon et al., 1991, Cell 67(23): 701--716 Ras1 and a putative guanine nucleotide exchange factor perform crucial steps in signaling by the sevenless protein tyrosine kinase. [FBrf0053392]

Allele Dmel\drke0A

Allele Dmel\drk^e0A