Allele Dmel\ex⁶⁹⁷

General Information
Symbol	Dmel\ex⁶⁹⁷	Species	D. melanogaster
Name		FlyBase ID	FBal0031223
Feature type	allele	Associated gene	Dmel\ex

Map ( GBrowse )	Untitled Document
Allele class
Mutagen	P-element activity
Recent Updates
Description	What does this section display? This section contains items that were added to this record for each release. It currently only tracks new links between this FlyBase report and other FlyBase data classes (e.g. genes, references, stocks) or controlled vocabulary terms (e.g. GO, anatomy terms). What does this section not display? This section does not currently display links that were removed or gene model changes.
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	P-element activity (Boedigheimer and Laughon, 1993)
Mutations Mapped to the Genome

Type Location Additional Notes References transposable element insertion site 2L:431,266..431,266 (Boedigheimer and Laughon, 1993, Maitra et al., 2006)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Insertion of P{lacW} at the 5'end of the ex transcription unit. (Boedigheimer and Laughon, 1993)
Caused by insertion	P{lacW}ex⁶⁹⁷ (Boedigheimer and Laughon, 1993, Maitra et al., 2006)
Cytology
Phenotypic Data
Phenotypic Class
	decreased cell size \| recessive (McCartney et al., 2000) increased cell number (Maitra et al., 2006) mitotic cell cycle defective (Maitra et al., 2006) visible \| recessive (McCartney et al., 2000)
Phenotype Manifest In
	anterior crossvein (McCartney et al., 2000) eye-antennal disc \| third instar larval stage (Maitra et al., 2006) interommatidial bristle & eye disc & pupa (Maitra et al., 2006) leg (Boedigheimer and Laughon, 1993) posterior crossvein (McCartney et al., 2000) thorax (Boedigheimer and Laughon, 1993) wing (Boedigheimer and Laughon, 1993, McCartney et al., 2000) wing disc (Maitra et al., 2006)
Detailed Description
	Statement Reference ex⁶⁹⁷ mutants do not exhibit any defect in photoreceptor differentiation. (Maitra et al., 2006) In ex⁶⁹⁷ third instar eye-antennal discs, many cells posterior to the second mitotic wave fail to exit the cell cycle. In ex⁶⁹⁷ pupal eye discs (24hrs after pupariation) exhibit extra interommatidial cells, often forming double rows surrounding the ommatidia. The number of cone cells does not change in the mutants. ex⁶⁹⁷ mutants exhibit overgrown wing imaginal discs. (Maitra et al., 2006) Homozygous females have a broad wing phenotype characterised by disruption of the posterior and anterior crossveins. The increased wing size appears to be due to an increase in cell number rather than an increase in cell size and in fact cell size is slightly decreased compared to wild type. (McCartney et al., 2000) Mild phenotype. (Boedigheimer et al., 1997) Broad, arced wings. Mutant wings have undergone at least one extra cell division. Legs, thorax and head may also be affected. (Boedigheimer and Laughon, 1993)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference ex⁶⁹⁷ has visible \| recessive phenotype, enhanceable by Mer¹/Mer[+] (McCartney et al., 2000)
Enhancer of
Statement Reference ex⁶⁹⁷/ex[+] is an enhancer of partially lethal - majority die \| larval stage phenotype of wts^P2 (Hamaratoglu et al., 2006) ex⁶⁹⁷ is an enhancer of visible \| recessive phenotype of Mer³ (McCartney et al., 2000)
Other
Statement Reference dpp^hr56, ex⁶⁹⁷ has visible \| recessive phenotype (McCartney et al., 2000) dpp^hr56, ex⁶⁹⁷ has visible phenotype (McCartney et al., 2000)
Phenotype Manifest In
Enhanced by
Statement Reference ex⁶⁹⁷ has wing phenotype, enhanceable by Mer¹/Mer[+] (McCartney et al., 2000)
Enhancer of
Statement Reference ex⁶⁹⁷/ex[+] is an enhancer of wing vein \| ectopic phenotype of Snr1^E1 (Marenda et al., 2004) ex⁶⁹⁷ is an enhancer of adult cuticle & head phenotype of Mer³ (McCartney et al., 2000) ex⁶⁹⁷ is an enhancer of eye phenotype of Mer³ (McCartney et al., 2000) ex⁶⁹⁷ is an enhancer of interommatidial bristle \| ectopic phenotype of Mer³ (McCartney et al., 2000) ex⁶⁹⁷ is an enhancer of interommatidial bristle phenotype of Mer³ (McCartney et al., 2000) ex⁶⁹⁷ is an enhancer of vibrissae \| ectopic phenotype of Mer³ (McCartney et al., 2000)
Other
Statement Reference dpp^hr56, ex⁶⁹⁷ has leg phenotype (McCartney et al., 2000, McCartney et al., 2000) dpp^hr56, ex⁶⁹⁷ has tarsal segment phenotype (McCartney et al., 2000, McCartney et al., 2000) Mer⁴, ex⁶⁹⁷ has adult eye primordium \| somatic clone phenotype (Maitra et al., 2006) Mer⁴, ex⁶⁹⁷ has antennal disc \| ectopic \| somatic clone phenotype (Maitra et al., 2006) Mer⁴, ex⁶⁹⁷ has antennal disc \| somatic clone phenotype (Maitra et al., 2006) Mer⁴, ex⁶⁹⁷ has eye photoreceptor cell \| somatic clone phenotype (Maitra et al., 2006) Mer⁴, ex⁶⁹⁷ has wing disc phenotype (Maitra et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference Mer⁴; ex⁶⁹⁷ double mutant eye-antennal imaginal discs have severely reduced eye primordia with a substantial reduction in or total absence of photoreceptors. In these mutants, photoreceptor development and overall eye field size are severely reduced. The antennal portion is normal or slightly larger than normal and in some cases is duplicated. Apoptosis does not seem to be affected. Mer⁴; ex⁶⁹⁷ somatic clones in contact with the posterior or lateral margin of the eye fail to produce photoreceptors. Those somatic clones located in the middle of the eye field can produce photoreceptors. (Maitra et al., 2006) Mer⁴; ex⁶⁹⁷ double mutants exhibit overgrown wing imaginal discs. (Maitra et al., 2006) The broad wing phenotype of ex⁶⁹⁷ homozygotes is dominantly enhanced by Mer¹. Dominantly enhances the head and broad wing phenotypes of Mer³ hemizygotes. ex⁶⁹⁷ dpp^hr56/ex⁶⁹⁷ flies have truncated legs in which the distal tarsal segments are absent (significant leg defects are not seen in either dpp^hr56/+ or ex⁶⁹⁷ single homozygote flies). (McCartney et al., 2000)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Dysgenesis induced excisions of the P element can be accompanied by reversion of the mutant phenotype. An allelic series can be defined for ex alleles with respect to viability, eclosion rate and penetrance of ex wing phenotype. Going from most to least severe: ex^e1 >= ex^l2ey > ex^e2 > ex^e6 > ex⁶⁹⁷ > ex¹. (Boedigheimer and Laughon, 1993)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	ex⁹ (Hamaratoglu et al., 2006) ex⁶⁹⁷ (Maitra et al., 2006, Hamaratoglu et al., 2006, Hamaratoglu et al., 2006, Willecke et al., 2006, Grusche et al., 2011)
Name Synonym
Secondary FlyBase IDs

References ( 10 )
Research paper	Grusche et al., 2011, Dev. Biol. 350(2): 255--266 The Salvador/Warts/Hippo pathway controls regenerative tissue growth in Drosophila melanogaster. [FBrf0212888] Hamaratoglu et al., 2006, Nat. Cell Biol. 8(1): 27--36 The tumour-suppressor genes NF2/Merlin and Expanded act through Hippo signalling to regulate cell proliferation and apoptosis. [FBrf0191261] Maitra et al., 2006, Curr. Biol. 16(7): 702--709 The tumor suppressors Merlin and Expanded function cooperatively to modulate receptor endocytosis and signaling. [FBrf0190031] Willecke et al., 2006, Curr. Biol. 16(21): 2090--2100 The fat cadherin acts through the hippo tumor-suppressor pathway to regulate tissue size. [FBrf0194966] Marenda et al., 2004, Dev. Biol. 267(2): 279--293 The Drosophila Brahma (SWI/SNF) chromatin remodeling complex exhibits cell-type specific activation and repression functions. [FBrf0174517] McCartney et al., 2000, Development 127(6): 1315--1324 The Neurofibromatosis-2 homologue, Merlin, and the tumor suppressor expanded function together in Drosophila to regulate cell proliferation and differentiation. [FBrf0125175] Boedigheimer et al., 1997, Dev. Genet. 20(2): 103--110 Expanded functions in the apical cell domain to regulate the growth rate of imaginal discs. [FBrf0093371] Boedigheimer and Laughon, 1993, Development 118(2): 1291--1301 expanded: a gene involved in the control of cell proliferation in Drosophila imaginal discs. [FBrf0058062]
Supplementary material	Hamaratoglu et al., 2006, Nat. Cell Biol. 8(1): The tumour-suppressor genes NF2/Merlin and Expanded act through Hippo signalling to regulate cell proliferation and apoptosis. [FBrf0205575] Maitra et al., 2006, Curr. Biol. 16(7): The tumor suppressors Merlin and expanded function cooperatively to modulate receptor endocytosis and signaling. [FBrf0199071]

Allele Dmel\ex697

Allele Dmel\ex⁶⁹⁷