FlyBase Allele Report: Dmel\ex[e1]

General Information

Symbol

Dmel\ex^e1

Species

D. melanogaster

Name

FlyBase ID

FBal0031224

Feature type

allele

Associated gene

Dmel\ex

Associated Insertion(s)

P{?lacW}ex^e1

Carried in Construct

Also Known As

ex-lacZ

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

loss of function allele

Mutagen

P-element activity

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Boedigheimer and Laughon, 1993)

loss of function allele

(Willecke et al., 2006, Boedigheimer et al., 1997)

Mutagen

P-element activity

(Boedigheimer and Laughon, 1993)

Progenitor genotype

ex⁶⁹⁷

(Boedigheimer and Laughon, 1993)

Associated Insertion(s)

P{?lacW}ex^e1

Cytology

Description

Imprecise excision of a P{lacW} insertion that leaves behind the Ecol\lacZ gene.

(Teleman and Cohen, 2006)

Small deletion that removes 3-12kb of genomic DNA, including the first ex exon.

(Boedigheimer and Laughon, 1993)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

deletion

2L:429,761..437,244 [+]

Linked to:

HindIII-HindIII restriction fragment

Comment:

Approximate mapping of ex^e1 deletion to a restriction fragment; ex^e1 consists of a partial excision of the original P{lacW}ex⁶⁹⁷ insert plus removal of 3-12kb of flanking genomic sequence; position of restriction fragment on reference sequence inferred by FlyBase curator

(Boedigheimer and Laughon, 1993)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 1 )

Disease

Evidence

References

model of cancer

CEA with Mer⁴

(Hamaratoglu et al., 2006)

Modifiers Based on Experimental Evidence ( 1 )

Disease

Interaction

References

ameliorates gastrointestinal adenoma

modeled by Apc^Q8, Apc2^g10

(Suijkerbuijk et al., 2016)

exacerbates Charcot-Marie-Tooth disease type 4B1

modeled by mtm^ΔN

(Hu et al., 2022)

modeled by mtm^ΔC

(Hu et al., 2022)

exacerbates cancer

modeled by Ras85D^V12.cUa.UAS

(Liu et al., 2022)

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal cell migration | oogenesis | somatic clone

(Badmos et al., 2021)

abnormal planar polarity | somatic clone

(Blaumueller and Mlodzik, 2000)

abnormal size | adult stage | somatic clone

(Zhang et al., 2015)

abnormal size | somatic clone | third instar larval stage

(Su et al., 2017)

decreased cell number | third instar larval stage

(Reddy and Irvine, 2011)

decreased size | adult stage

(Yang and Choi, 2021)

hyperplasia

(Ziosi et al., 2010)

hyperplasia | recessive

(Boedigheimer and Laughon, 1993)

increased cell number | cell non-autonomous | somatic clone

(Sun et al., 2008)

increased cell number | larval stage

(Cho et al., 2006)

increased size | adult stage | somatic clone - tissue specific

(Hu et al., 2022)

lethal

(Tokamov et al., 2021, Gaspar et al., 2015)

lethal (with Df(2L)BSC107)

(Kahsai et al., 2016)

lethal | recessive

(Blaumueller and Mlodzik, 2000, McCartney et al., 2000, Boedigheimer et al., 1997)

lethal - all die before end of P-stage | recessive

(Boedigheimer and Laughon, 1993)

neoplasia | adult stage | cell non-autonomous | male | somatic clone

(Sun et al., 2008)

some die during pharate adult stage | recessive

(Boedigheimer and Laughon, 1993)

visible | adult stage

(Yang and Choi, 2021)

visible | adult stage | somatic clone

(Hu et al., 2016, Gaspar et al., 2015)

visible | somatic clone

(Baumgartner et al., 2010, Genevet et al., 2010)

Phenotype Manifest In

adult head | somatic clone

(Zhang et al., 2015)

antenna

(Boedigheimer and Laughon, 1993)

antenna | adult stage | somatic clone

(Hu et al., 2016)

arista

(Boedigheimer and Laughon, 1993)

border follicle cell | somatic clone

(Badmos et al., 2021)

eye | adult stage | somatic clone

(Hu et al., 2016, Gaspar et al., 2015)

eye | somatic clone

(Zhang et al., 2015, Baumgartner et al., 2010, Genevet et al., 2010, Blaumueller and Mlodzik, 2000)

eye | somatic clone - tissue specific

(Hu et al., 2022)

eye-antennal disc

(Boedigheimer and Laughon, 1993)

eye disc

(Blaumueller and Mlodzik, 2000)

eye disc | somatic clone

(Blaumueller and Mlodzik, 2000)

eye disc | third instar larval stage

(Gaspar et al., 2015)

haltere disc

(Boedigheimer and Laughon, 1993)

head

(Boedigheimer and Laughon, 1993)

interommatidial bristle | somatic clone

(Ling et al., 2010)

interommatidial cell | pupal stage | somatic clone | increased number

(Gaspar et al., 2015)

larval glial cell | third instar larval stage

(Reddy and Irvine, 2011)

leg

(Boedigheimer and Laughon, 1993)

mesothoracic leg disc

(Boedigheimer and Laughon, 1993)

mesothoracic tarsal segment 3

(Boedigheimer and Laughon, 1993)

mesothoracic tarsal segment 4

(Boedigheimer and Laughon, 1993)

mesothoracic tarsal segment 5

(Boedigheimer and Laughon, 1993)

metathoracic leg disc

(Boedigheimer and Laughon, 1993)

metathoracic tarsal segment 3

(Boedigheimer and Laughon, 1993)

metathoracic tarsal segment 4

(Boedigheimer and Laughon, 1993)

metathoracic tarsal segment 5

(Boedigheimer and Laughon, 1993)

ommatidium

(Boedigheimer and Laughon, 1993)

ommatidium | somatic clone

(Blaumueller and Mlodzik, 2000)

photoreceptor neuron | somatic clone

(Blaumueller and Mlodzik, 2000)

prothoracic leg disc

(Boedigheimer and Laughon, 1993)

prothoracic tarsal segment 3

(Boedigheimer and Laughon, 1993)

prothoracic tarsal segment 4

(Boedigheimer and Laughon, 1993)

prothoracic tarsal segment 5

(Boedigheimer and Laughon, 1993)

spermatogonial cyst | cell non-autonomous | somatic clone

(Sun et al., 2008)

testis | adult stage | cell non-autonomous | somatic clone

(Sun et al., 2008)

wing

(Yang and Choi, 2021, Boedigheimer and Laughon, 1993)

wing | somatic clone

(Zhang et al., 2015)

wing disc

(Boedigheimer and Laughon, 1993)

wing disc | larval stage

(Cho et al., 2006)

wing disc | somatic clone | third instar larval stage

(Su et al., 2017)

wing disc | third instar larval stage (with ex^AP50)

(Gaspar et al., 2015)

Detailed Description

Statement

Reference

Follicle border cell clusters either fully clonal or mosaic for ex^e1/ex^e1 show delayed migration.

(Badmos et al., 2021)

The relative size of somatic MARCM clones mutant for ex^e1 in third instar larval wing discs is significantly increased compared to control clones in wild-type discs.

(Su et al., 2017)

Adult eyes containing ex^e1 homozygous somatic clones (induced by the eyFLP method) display overgrowth and folding phenotype not only in the eyes but also at the base of antennae.

(Hu et al., 2016)

ex^e1/+ does not have any affect on cell growth or proliferation in the adult posterior midgut, as compared to controls.

(Suijkerbuijk et al., 2016)

ex^e1/ex^e1 eyes (created by the EGUF method in otherwise heterozygous animals) contain significantly increased number of interomatidial cells (IOC) in the pupal retina compared to wild-type and the adult eyes are smaller and misshapen.

ex^e1/ex^e1 mutants rarely survive to third instar larval stage (but never to adulthood) and the survivors display decreased number of photoreceptor cells in the eye disc compared to wild-type.

The wing discs of ex^e1/ex^AP50 transheterozygotes are overgrown compared to controls.

(Gaspar et al., 2015)

ex^e1/ex^e1 mosaic adult wings are enlarged in size relative to controls. ex^e1/ex^e1 mosaic female heads exhibit both head and eye overgrowth as compared to controls.

(Zhang et al., 2015)

Cells in homozygous clones in the wing disc accumulate F-actin near the apical surface.

(Fernández et al., 2011)

ex^e1 mutant larvae show a decrease in the number of glial cells in the eye disc and a lack of glial overgrowth.

(Reddy and Irvine, 2011)

Eyes that are partially homozygous for ex^e1 (generated using the eyFLP method without cell lethal) show mild overgrowth compared to controls.

(Baumgartner et al., 2010)

Pupal retinae composed of homozygous ex^e1 mutant cells show an increase in the number of interommatidial cells.

(Ling et al., 2010)

ex^e1 homozygous mutant clones generated in the male germline develop normally. 16 cells are observed per cyst, and cell size and morphology are indistinguishable from neighbouring control cells. However, non-autonomous germline over-proliferation is observed in non-mutant spermatogonial cysts.

(Sun et al., 2008)

ex^e1 mutant wing discs collected 36-48 hours after the L2-L3 molt are overgrown.

(Cho et al., 2006)

ex^e1 mutants do not exhibit any defect in photoreceptor differentiation.

(Maitra et al., 2006)

Mutant ex^e1 clones, generated through FLP-induced recombination are significantly larger than their wild-type twin-spots at the larval stage.

ex^e1 pupal retinas exhibit an increase in secondary cells that are normally eliminated by apoptosis (approximately 8 in ex^e1 clones, compared to 6 in wild-type).

(Silva et al., 2006)

Somatic clones of homozygous ex^e1 tissue in the eye exhibit ommatidial chirality inversions, misrotations and minor defects in photoreceptor differentiation. Somatic clones in the pupal imaginal disc produces a disruption of the well-ordered pattern of R3/R4 photoreceptor precursor cells. Clones also exhibit a significant growth advantage over the wild-type counterparts in larval and pupal discs, and in the adult eye. In the case of large clones the tissue protrudes out of the plane of the disc. Homozygous ex^e1 animals survive until the pharate adult stage. The eye discs are disproportionately large in comparison with the antennal discs of the same complex, reaching several times the size of wild-type larvae. Anterior regions of mutant discs lose their 'flat' character, leading to the formation of additional tissue flaps. In these discs the morphogenetic furrow moves across the mutant tissue and cell fate determination does take place.

(Blaumueller and Mlodzik, 2000)

Clonal analysis revealed no effect on tissues other than the wing. Mutant clones generated 3-5 days AEL are more than twice as large as their wild type twins, but show no significant differences depending on the position in the wing. ex function is not required at or after 5-6 days AEL.

(Boedigheimer et al., 1997)

Pharate adults have a massive head, wing and leg defects. Most common leg defect is missing distal tarsal segments including claw organ, with the remaining proximal tarsal segments have supernumerary bristles. Wing disc of third instar larvae is enlarged, and by day 5 has formed an extra fold. Overgrowth also occurs in the haltere discs, and the eye disc and leg discs show signs of degeneration.

(Boedigheimer and Laughon, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

ex^e1 has visible | somatic clone phenotype, enhanceable by kibra^del

(Yu et al., 2010)

ex^e1 has hyperplasia phenotype, enhanceable by Myc^UAS.cZa/Scer\GAL4^hh.PU

(Ziosi et al., 2010)

Suppressed by

Statement

Reference

ex^e1, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Pi3K68D^HMS01296

(Hu et al., 2022)

ex^e1, mtm^null has increased size | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Pi3K68D^HMS01296

(Hu et al., 2022)

ex^e1, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Rok^HMS01311/Scer\GAL4^αTub84B.PL

(Hu et al., 2022)

ex^e1, mtm^null has increased size | somatic clone | adult stage phenotype, suppressible by Rok^HMS01311/Scer\GAL4^αTub84B.PL

(Hu et al., 2022)

ex^e1, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP}

(Hu et al., 2022)

ex^e1, mtm^null has increased size | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP}

(Hu et al., 2022)

ex^e1, mtm^null has increased cell number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Hsap\MTMR2^UAS.cHa

(Hu et al., 2022)

ex^e1, mtm^null has increased size | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Hsap\MTMR2^UAS.cHa

(Hu et al., 2022)

ex^e1 has increased mortality during development phenotype, suppressible by kibra^{Ubi-p63E.GFP,Tag:FLAG}

(Tokamov et al., 2021)

ex^e1 has abnormal size | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/kibra^UASp.cGa

(Su et al., 2017)

ex^e1 has visible | adult stage phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has lethal phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has abnormal size | somatic clone | adult stage phenotype, suppressible by tai^61G1/tai^61G1

(Zhang et al., 2015)

ex^e1 has visible | somatic clone phenotype, suppressible by kibra^EP747/Scer\GAL4^Tub.PU

(Genevet et al., 2010)

NOT suppressed by

Statement

Reference

ex^e1 has visible | somatic clone | adult stage phenotype, non-suppressible by Ack^10b/Ack^10b

(Hu et al., 2016)

ex^e1 has visible | somatic clone | adult stage phenotype, non-suppressible by Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU

(Hu et al., 2016)

ex^e1 has increased cell number | larval stage phenotype, non-suppressible by dachs^GC13

(Cho et al., 2006)

Enhancer of

Statement

Reference

ex^e1/ex^e1 is an enhancer of increased size | adult stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

ex^e1/ex^e1 is an enhancer of increased cell number | P-stage | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

ex^e1 is an enhancer of visible | somatic clone phenotype of kibra^del

(Yu et al., 2010)

ex[+]/ex^e1 is an enhancer of partially lethal - majority die phenotype of ft⁸/ft^G-rv

(Silva, 2006)

ex[+]/ex^e1 is an enhancer of visible phenotype of dsh^hs.sev.B

(Blaumueller and Mlodzik, 2000)

ex^e1 is an enhancer of visible | recessive phenotype of Mer³

(McCartney et al., 2000)

Suppressor of

Statement

Reference

ex^e1 is a suppressor of decreased size | adult stage phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

ex^e1 is a suppressor of visible | adult stage phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

ex^e1/ex^e1 is a suppressor | somatic clone of abnormal size | somatic clone | third instar larval stage phenotype of Scer\GAL4^Tub.PU, kibra^UASp.cGa

(Su et al., 2017)

ex[+]/ex^e1 is a suppressor of decreased cell number | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

ex[+]/ex^e1 is a suppressor of increased cell number | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

Other

Statement

Reference

ex^e1, mtm^null has increased size | somatic clone | adult stage phenotype

(Hu et al., 2022)

Rab11^{S25N.UASp.YFP}, Scer\GAL4^αTub84B.PL, ex^e1 has increased cell number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔC has increased size | adult stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔC has increased cell number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔN has increased size | adult stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔN has increased cell number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has fertile phenotype

(Tokamov et al., 2021)

ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has visible | adult stage phenotype

(Tokamov et al., 2021)

ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has increased size | adult stage phenotype

(Tokamov et al., 2021)

ex[+]/ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has visible | adult stage phenotype

(Tokamov et al., 2021)

ex[+]/ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has decreased size | adult stage phenotype

(Tokamov et al., 2021)

ex^e1, ft⁸ has visible | somatic clone phenotype

(Dent et al., 2015)

ex^e1, ft⁸ has partially lethal - majority die | somatic clone phenotype

(Dent et al., 2015)

RtGEF^p1036, ex^e1, ft⁸ has lethal | somatic clone phenotype

(Dent et al., 2015)

ex^e1, kibra¹/kibra³ has lethal | somatic clone | pupal stage phenotype

(Baumgartner et al., 2010)

Myc^UAS.cZa, Scer\GAL4^hh.PU, ex^e1 has increased cell size phenotype

(Ziosi et al., 2010)

Mer⁴, ex^e1 has visible phenotype

(Hamaratoglu et al., 2006)

Mer⁴, ex^e1 has hyperplasia | somatic clone phenotype

(Hamaratoglu et al., 2006)

Mer⁴, ex^e1 has visible | recessive | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has neoplasia | somatic clone | adult stage phenotype

(McCartney et al., 2000)

Phenotype Manifest In

Enhanced by

Statement

Reference

ex^e1 has eye | somatic clone phenotype, enhanceable by kibra^del

(Yu et al., 2010)

ex^e1 has ommatidium | somatic clone phenotype, enhanceable by kibra^del

(Yu et al., 2010)

NOT Enhanced by

Statement

Reference

ex^e1 has eye | adult stage | somatic clone phenotype, non-enhanceable by Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU

(Hu et al., 2016)

ex^e1 has antenna | adult stage | somatic clone phenotype, non-enhanceable by Ack^UAS.Tag:V5/Scer\GAL4^Ubi.PU

(Hu et al., 2016)

ex^e1 has interommatidial bristle | somatic clone phenotype, non-enhanceable by crb^82-04

(Ling et al., 2010)

ex^e1 has phenotype, non-enhanceable by Rho1^V14.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 has phenotype, non-enhanceable by Rac1^V12.hs.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 has phenotype, non-enhanceable by fz^hs.sev

(Blaumueller and Mlodzik, 2000)

Suppressed by

Statement

Reference

ex^e1, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Pi3K68D^HMS01296

(Hu et al., 2022)

ex^e1, mtm^null has mesonotum | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Pi3K68D^HMS01296

(Hu et al., 2022)

ex^e1, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Rok^HMS01311/Scer\GAL4^αTub84B.PL

(Hu et al., 2022)

ex^e1, mtm^null has mesonotum | somatic clone | adult stage phenotype, suppressible by Rok^HMS01311/Scer\GAL4^αTub84B.PL

(Hu et al., 2022)

ex^e1, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP}

(Hu et al., 2022)

ex^e1, mtm^null has mesonotum | somatic clone | adult stage phenotype, suppressible by Scer\GAL4^αTub84B.PL/Rab11^{Q70L.UASp.YFP}

(Hu et al., 2022)

ex^e1, mtm^null has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Hsap\MTMR2^UAS.cHa

(Hu et al., 2022)

ex^e1, mtm^null has mesonotum | somatic clone | adult stage phenotype, suppressible | partially by Scer\GAL4^αTub84B.PL/Hsap\MTMR2^UAS.cHa

(Hu et al., 2022)

ex^e1 has wing disc | somatic clone | third instar larval stage phenotype, suppressible by Scer\GAL4^Tub.PU/kibra^UASp.cGa

(Su et al., 2017)

ex^AP50/ex^e1 has wing disc | third instar larval stage phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has eye | somatic clone | adult stage phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has interommatidial cell | increased number | somatic clone | pupal stage phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has eye disc | third instar larval stage phenotype, suppressible by Zyx^Δ41/Zyx^Δ41

(Gaspar et al., 2015)

ex^e1 has wing | somatic clone phenotype, suppressible by tai^61G1/tai^61G1

(Zhang et al., 2015)

ex^e1 has adult head | somatic clone phenotype, suppressible by tai^61G1/tai^61G1

(Zhang et al., 2015)

ex^e1 has eye | somatic clone phenotype, suppressible by tai^61G1/tai^61G1

(Zhang et al., 2015)

ex^e1 has eye | somatic clone phenotype, suppressible by kibra^EP747/Scer\GAL4^Tub.PU

(Genevet et al., 2010)

NOT suppressed by

Statement

Reference

ex^e1 has eye | somatic clone | adult stage phenotype, non-suppressible by Ack^10b/Ack^10b

(Hu et al., 2016)

ex^e1 has antenna | somatic clone | adult stage phenotype, non-suppressible by Ack^10b/Ack^10b

(Hu et al., 2016)

ex^e1 has interommatidial bristle | somatic clone phenotype, non-suppressible by crb^82-04

(Ling et al., 2010)

ex^e1 has wing disc | larval stage phenotype, non-suppressible by dachs^GC13

(Cho et al., 2006)

ex^e1 has phenotype, non-suppressible by Rac1^V12.hs.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 has phenotype, non-suppressible by Rho1^V14.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 has phenotype, non-suppressible by fz^hs.sev

(Blaumueller and Mlodzik, 2000)

Enhancer of

Statement

Reference

ex^e1/ex^e1 is an enhancer of eye | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

ex^e1/ex^e1 is an enhancer of interommatidial cell | P-stage | increased number | somatic clone - tissue specific phenotype of mtm^null

(Hu et al., 2022)

ex[+]/ex^e1 is an enhancer of wing phenotype of pyd^tam/pyd^ex147

(Djiane et al., 2011)

ex^e1 is an enhancer of eye | somatic clone phenotype of kibra^del

(Yu et al., 2010)

ex^e1 is an enhancer of ommatidium | somatic clone phenotype of kibra^del

(Yu et al., 2010)

ex^e1 is an enhancer of interommatidial bristle | increased number phenotype of Mer⁴

(Willecke et al., 2006)

ex[+]/ex^e1 is an enhancer of ommatidium phenotype of dsh^hs.sev.B

(Blaumueller and Mlodzik, 2000)

ex[+]/ex^e1 is an enhancer of eye photoreceptor cell phenotype of dsh^hs.sev.B

(Blaumueller and Mlodzik, 2000)

ex[+]/ex^e1 is an enhancer of eye phenotype of dsh^hs.sev.B

(Blaumueller and Mlodzik, 2000)

ex^e1 is an enhancer of adult cuticle & head phenotype of Mer³

(McCartney et al., 2000)

NOT Enhancer of

Statement

Reference

ex^e1 is a non-enhancer of phenotype of Rho1^V14.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 is a non-enhancer of phenotype of fz^hs.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 is a non-enhancer of phenotype of Rac1^V12.hs.sev

(Blaumueller and Mlodzik, 2000)

Suppressor of

Statement

Reference

ex^e1 is a suppressor of wing phenotype of Patronin^GD11946, Scer\GAL4^en.PU

(Yang and Choi, 2021)

ex^e1/ex^e1 is a suppressor | somatic clone of wing disc | somatic clone | third instar larval stage phenotype of Scer\GAL4^Tub.PU, kibra^UASp.cGa

(Su et al., 2017)

ex[+]/ex^e1 is a suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone | cell non-autonomous phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

ex[+]/ex^e1 is a suppressor of intestinal stem cell of posterior adult midgut epithelium | somatic clone phenotype of Apc2^g10, Apc^Q8

(Suijkerbuijk et al., 2016)

NOT Suppressor of

Statement

Reference

ex^e1 is a non-suppressor of phenotype of Rac1^V12.hs.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 is a non-suppressor of phenotype of Rho1^V14.sev

(Blaumueller and Mlodzik, 2000)

ex^e1 is a non-suppressor of phenotype of fz^hs.sev

(Blaumueller and Mlodzik, 2000)

Other

Statement

Reference

ex^e1, mtm^null has mesonotum | somatic clone | adult stage phenotype

(Hu et al., 2022)

ex^e1, mtm^null has eye | somatic clone - tissue specific phenotype

(Hu et al., 2022)

Rab11^{S25N.UASp.YFP}, Scer\GAL4^αTub84B.PL, ex^e1 has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔC has eye | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔC has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔN has eye | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, mtm^ΔN has interommatidial cell | increased number | P-stage | somatic clone - tissue specific phenotype

(Hu et al., 2022)

ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has wing phenotype

(Tokamov et al., 2021)

ex[+]/ex^e1, kibra^{Ubi-p63E.GFP,Tag:FLAG} has wing phenotype

(Tokamov et al., 2021)

ex^e1, ft⁸ has ptilinum | somatic clone phenotype

(Dent et al., 2015)

ex^e1, tai^61G1 has wing disc | third instar larval stage phenotype

(Zhang et al., 2015)

ex^e1, kibra^Δ32/kibra[+] has follicle cell phenotype

(Fletcher et al., 2012)

ex^e1, kibra¹/kibra³ has head | somatic clone | pupal stage phenotype

(Baumgartner et al., 2010)

ex^e1, kibra¹ has eye disc | somatic clone phenotype

(Baumgartner et al., 2010)

ex^e1, ft⁸ has interommatidial cell | increased number | pupal stage phenotype

(Bennett and Harvey, 2006)

Mer⁴, ex^e1 has interommatidial cell | somatic clone | pupal stage phenotype

(Hamaratoglu et al., 2006)

Mer⁴, ex^e1 has eye photoreceptor cell | somatic clone phenotype

(Maitra et al., 2006)

Df(1)N-54l9/Mer⁴, ex^e1 has wing | somatic clone phenotype

(Maitra et al., 2006)

Mer⁴, ex^e1 has ommatidium | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has eye | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has wing vein | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has posterior crossvein | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has wing cell | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has adult head & cuticle | ectopic | somatic clone phenotype

(McCartney et al., 2000)

Mer⁴, ex^e1 has eye disc morphogenetic furrow | larval stage phenotype

(McCartney et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The increased relative size of somatic MARCM clones mutant for ex^e1 in third instar larval wing discs (as compared to wild-type clones in control discs) is suppressed by expression of kibra^{Scer\UAS.P\T.cGa} in the mutant clones (driven by Scer\GAL4^tub.PU), which on its own produces clones of reduced size.

(Su et al., 2017)

The overgrowth phenotype observed in adult eye and antennae containing ex^e1 homozygous mutant somatic clones (induced by the eyFLP method) is not changed when the clones are induced in Ack^10b/Ack^10b mutant background or when they also express Ack^{Scer\UAS.T:SV5\V5} under the control of Scer\GAL4^Ubi.PU.

(Hu et al., 2016)

ex^e1/+ fully suppresses the reduced growth seen in posterior midgut wild type clones when in the presence of Apc^Q8/Apc^Q8, Apc2^g10/Apc2^g10 mutant clones, and suppresses the growth of Apc^Q8/Apc^Q8, Apc2^g10/Apc2^g10 mutant clones.

(Suijkerbuijk et al., 2016)

Double homozygous ft⁸, ex^e1 clones generated using the eyFLP system result in substantial overgrowth of the head, particularly in the ptilinum.

(Dent et al., 2015)

The supernumerary inter-ommatidial cells in the pupal retina as well as the smaller size and altered eye shape in adults characteristic for ex^e1/ex^e1 eyes (created by the EGUF method in otherwise heterozygous animals) can be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

The low survival rate of ex^e1/ex^e1 mutants to third instar larval stage can be improved by combination with Zyx^Δ41/Zyx^Δ41 and some of the double mutants can survive even to adulthood. The decreased number of photoreceptor cells in the eye discs characteristic for ex^e1/ex^e1 mutants is also restored in the surviving ex^e1;Zyx^Δ41 double mutant third instar larvae.

The overgrowth of the wing discs characteristic for ex^e1/ex^AP50 transheterozygotes can be suppressed by combination with Zyx^Δ41/Zyx^Δ41.

(Gaspar et al., 2015)

tai^61G1/tai^61G1, ex^e1/ex^e1 mosaic adult wings are smaller than ex^e1/ex^e1 mosaic wings, although the wings are still a broader shape than controls; tai^61G1/tai^61G1, ex^e1/ex^e1 clones in the L3 wing disc appear smaller than age-matched ex^e1/ex^e1 clones; tai^61G1/tai^61G1, ex^e1/ex^e1 mosaic heads have suppressed head and eye overgrowth as compared to ex^e1/ex^e1 clones.

(Zhang et al., 2015)

ex^e1/ex^e1, kibra^Δ32/+ egg chambers develop a multilayered follicle cell epithelium.

(Fletcher et al., 2012)

Wings from pyd^ex147/pyd^tam flies are broader compared to control wings, and this phenotype is enhanced in a ex^e1/+ background.

(Djiane et al., 2011)

Expression of yki^NIG.4005R under the control of Scer\GAL4^unspecified in ex^e1 clones in the wing disc does not prevent apical accumulation of F-actin in the mutant cells.

(Fernández et al., 2011)

Pupae with mosaic heads that are largely doubly mutant for ex^e1/ex^e1 and kibra¹/kibra³ (clones induced using the eyFLP method without cell lethal) do not eclose and normal head structures are displaced by overgrown tissue.

ex^e1 kibra¹ double mutant clones in the eye imaginal disc are very large and invariably adopt a rounded shape.

(Baumgartner et al., 2010)

Pupal retinae composed of crb^82-04 ex^e1 double mutant cells show a similar number of interommatidial cells as do the single mutants.

(Ling et al., 2010)

ex^e1 ; kibra^del double mutant clones in the eye show a more severe eye overgrowth phenotype and a greater number of interommatidial cells per ommatidium than is seen in either single mutant.

(Yu et al., 2010)

dm^Scer\UAS.cZa overexpression clones (under the control of Scer\GAL4^hh.PU) found in the posterior of the wing disc strongly enhance the proliferative activity of ex^e1 mutant cells. In addition these cells are larger in dm^Scer\UAS.cZa clones than in a wild-type background.

(Ziosi et al., 2010)

ex^e1 allows recovery of Df(1)su(s)R194/+ clones in the adult eye in animals with mosaic eyes containing two genotypes of cells with respect to RpL36; cells which are Df(1)su(s)R194/+ and cells in which the haplo-insufficiency of Df(1)su(s)R194/+ for RpL36 has been rescued by RpL36^+t4 (in a wild-type background the Df(1)su(s)R194/+ clones are eliminated by cell competition and are not seen in the adult eye in these animals).

(Tyler et al., 2007)

d^GC13 does not suppress the wing disc overgrowth seen in ex^e1 mutant larvae.

(Cho et al., 2006)

Somatic clones homozygous for Mer⁴ and ex^e1 in the antenna or in the dorsal thorax are massively overgrown. In the mid-pupal retina, these clones contain a large excess of inter-ommatidial cells. In the late third instar eye disc, cells in these clones show increased levels of mitosis after (posterior to) the second mitotic wave. Later, at around 25 hours APF, the widespread apotosis seen throughout the developing retina in wild-type and heterozygous cells, is largely suppressed in these clones.

(Hamaratoglu et al., 2006)

Mer⁴; ex^e1 somatic clones in contact with the posterior or lateral margin of the eye fail to produce photoreceptors. Those somatic clones located in the middle of the eye field can produce photoreceptors.

Mer⁴; ex^e1 double mutant clones exhibit defects in membrane trafficking of proteins such as N and Egfr.

Df(1)N-54l9 Mer⁴; ex^e1 triple transheterozygous mutants suppress the Df(1)N-54l9 heterozygous wing notch phenotype.

(Maitra et al., 2006)

ft⁸/ft^G-rv ex^e1 mutant retinas exhibit an increase in the number of secondary cells per ommatidia (approximately 9, compared to 6 in wild-type), that is not statistically significant from ft⁸ single mutants.

(Silva et al., 2006)

One copy of ex^e1 enhances the lethality of ft⁸/ft^G-rv animals. Those that are wild-type for ex^e1 but mutant for ft⁸/ft^G-rv emerge as 0.32% of the progeny, whereas those that are also mutant for ex^e1 emerge as 0.269% of the progeny, a small, but statistically significant difference.

(Silva, 2006)

ex^e1;Mer⁴ double mutants exhibit a large excess of interommatidial cells, in a very similar manner to hpo mutants.

(Willecke et al., 2006)

The eye phenotype seen in dsh^hs.sev.B flies is dominantly enhanced by the addition of ex^e1 due to an increase in unscorable ommatidia (missing one or more photoreceptors.

(Blaumueller and Mlodzik, 2000)

Dominantly enhances the head phenotype of Mer³ hemizygotes. Both vein and intervein cells can differentiate in Mer⁴; ex^e1 double mutant clones in the wing. Clones that intersect the position of the posterior crossvein disrupt its development. Clones in the position of the anterior crossvein develop normally. Within the mutant intervein and vein clones, apparent defects in proliferation control are seen; in the proximal region of the wing, clonal vein tissue forms a raised protrusion. In other regions of the wing, bulges in the veins are also seen, although more frequently vein clones are merely broadened when compared with the surrounding vein. In the intervein regions, the clonal tissue appears to bulge and crinkle within the confines of the normal tissue, suggesting overproliferation. Cells within the intervein clones appear to differentiate as intervein cells, however, the cuticle deposited at the base of each wing hair within the clone appears to be thickened, and is distinct from cuticle produced by either the surrounding heterozygous intervein or vein cells. Mer⁴; ex^e1 double mutant clones in the eye appear to disrupt the progression of the morphogenetic furrow, being seen either as small scars with associated clusters of bristles or as elongated scars and associated indentations running from within the eye field towards the anterior margin. These clones do not differentiate ommatidia. The clones are often associated with overproliferated head cuticle.

(McCartney et al., 2000)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

ex^NY1

(Tyler et al., 2007)

Rescued by

ex^e1 is rescued by Scer\GAL4^32B/ex^UAS.cBa

(Boedigheimer et al., 1997)

Comments

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

44249

w^*; ex^e1 P{neoFRT}40A/CyO

Notes on Origin

Discoverer

Comments

An allelic series can be defined for ex alleles with respect to viability, eclosion rate and penetrance of ex wing phenotype. Going from most to least severe: ex^e1 >= ex^l2ey > ex^e2 > ex^e6 > ex⁶⁹⁷ > ex¹.

(Boedigheimer and Laughon, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

ex^e1

(Guo et al., 2024, Fulford et al., 2023, Hu et al., 2022, Liu et al., 2022, Wang et al., 2022, Badmos et al., 2021, Tokamov et al., 2021, Yang and Choi, 2021, Aguilar-Aragon et al., 2020, Wang and Baker, 2019, Yu and Pan, 2018, Anderson et al., 2017, Pascual et al., 2017, Su et al., 2017, Zheng et al., 2017, Boone et al., 2016, Hu et al., 2016, Dent et al., 2015, Gailite et al., 2015, Gaspar et al., 2015, Jia et al., 2015, Parker and Struhl, 2015, Vrabioiu and Struhl, 2015, Wang and Baker, 2015, Zhang et al., 2015, Zhang et al., 2015, Fletcher et al., 2012, Djiane et al., 2011, Fernández et al., 2011, Grusche et al., 2011, Sansores-Garcia et al., 2011, Baumgartner et al., 2010, Baumgartner et al., 2010, Genevet et al., 2010, Ling et al., 2010, Yu et al., 2010, Yu et al., 2010, Ziosi et al., 2010, Genevet et al., 2009, Oh and Irvine, 2008, Sun et al., 2008, Pellock et al., 2007, Tyler et al., 2007, Bennett and Harvey, 2006, Cho et al., 2006, Hamaratoglu et al., 2006, Maitra et al., 2006, Polesello et al., 2006, Polesello et al., 2006, Silva, 2006, Silva et al., 2006, Willecke et al., 2006)

ex^ex1

(Suijkerbuijk et al., 2016)

Name Synonyms

Secondary FlyBase IDs

References (62)

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