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General Information
Symbol
Dmel\fafFO8
Species
D. melanogaster
Name
FlyBase ID
FBal0031258
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
fafF08
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C31785557T
Reported nucleotide change:
C?T
Amino acid change:
H1986Y | faf-PA; H1986Y | faf-PC; H1986Y | faf-PD; H1986Y | faf-PE; H1986Y | faf-PG; H1986Y | faf-PH; H1986Y | faf-PI
Reported amino acid change:
H1986Y
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Nucleotide substitution: C?T. Amino acid replacement: H1986Y.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
eye photoreceptor cell & ommatidium | ectopic | somatic clone
Detailed Description
Statement
Reference
Only 27% of the expected number of fafFO8/fafB3 zygotic mutants survive. The embryos have a ventralised cuticle with dorsally extended denticles and an ectopic denticle belt replacing the Filzkorper. fafFO8/Df(3R)faf-BP embryos have a ventralised cuticle with dorsally extended denticles and poorly developed Filzkorper. Embryos derived from fafB4/fafFO8 females mated to wild-type males show significant ventralisation of the cuticle, with a herniated head, defective Filzkorper, malformed dorsal cuticle and denticle belts that extend well into the dorsal half of the embryo.
fafFO8 homozygous somatic clones in the eye lead to the formation of ommatidia containing one additional photoreceptor cell. For an ommatidium to show this phenotype, it is sufficient for 2 of the photoreceptors R2 to R5 to be mutant.
fafBX3/fafFO8 eyes are nearly wild type.
The external surface of the eye is irregular in fafFO8/fafBX4 flies and most facets contain more than the wild-type number of photoreceptor cells.
fafBX4/fafFO8 flies have a rough eye phenotype, due to the ommatidia containing more than the normal number of photoreceptor cells. Embryos derived from homozygous females die prior to cellularisation. fafBX3/fafFO8 flies have facets with more than the wild-type number of photoreceptor cell and an abnormal number of cone cells.
Each facet contains more than the normal number of photoreceptors in mutant flies. BrdU incorporation resembles wild type in fafFO8/fafBX4 third larval instar eye discs.
fafFO8/fafBX3 flies have essentially wild-type eyes.
fafBX3/fafFO8 adults exhibit nearly wild type eyes with a smooth exterior.
Most facets of transheterozygotes with fafBX3 contain more than the normal complement of 8 photoreceptor cells, many facets have extra R7 cells and a small proportion have missing R7 cells.
Mutation causes no significant alteration to the variegated eye phenotype of In(1)wm4h.
Mutation has no effect on rhohs.sev rough eye phenotype.
Eyes roughened due to abnormalities in the hexagonal facet array: most facets include 1-3 extra outer photoreceptor cells which are probably mystery cells. Ovaries of homozygous females are normal in appearance but the embryos produced never form syncytial blastoderm and all fail to hatch.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
fafFO8/fafBX3 has visible phenotype, enhanceable by shi1/shi1
fafFO8/fafBX3 has visible phenotype, enhanceable by shi2/shi2
fafFO8/fafBX3 has visible phenotype, enhanceable by lqfAG/lqf[+]
fafFO8/fafBX3 has visible phenotype, enhanceable by Chc[+]/Chc1
fafFO8/fafBX3 has visible phenotype, enhanceable by shiEM42/shi[+]
fafFO8/fafBX3 has visible phenotype, enhanceable by shi[+]/shiEM45
Suppressed by
Statement
Reference
fafFO8/fafBX3, lqfbE25 has visible phenotype, suppressible by effXS347
fafFO8/fafBX3, lqfbE25 has visible phenotype, suppressible by eff01462
fafFO8/fafBX3, lqfbE25 has visible phenotype, suppressible by Prosβ61
fafFO8/fafBX4 has visible phenotype, suppressible by Mmus\Usp9x+.ro
fafFO8/fafBX4 has visible phenotype, suppressible by Mmus\Usp9x-.ro
fafFO8 has visible | recessive phenotype, suppressible by S(faf)244[+]/S(faf)244244
fafFO8 has visible | recessive phenotype, suppressible by S(faf)8686/S(faf)86[+]
fafFO8 has visible | recessive phenotype, suppressible by eff[+]/effSfaf184
fafFO8 has visible | recessive phenotype, suppressible by DNAprim[+]/DNApol-α60j10B2
fafFO8 has visible | recessive phenotype, suppressible by Df(3L)rdgC-co2/+
fafFO8 has visible | recessive phenotype, suppressible by DNApol-α60Sfaf240/DNAprim[+]
fafFO8 has visible | recessive phenotype, suppressible by S(faf)196196/S(faf)196[+]
NOT suppressed by
Statement
Reference
fafFO8 has female sterile | recessive phenotype, non-suppressible by S(faf)8686/S(faf)86[+]
fafFO8 has female sterile | recessive phenotype, non-suppressible by eff[+]/effSfaf184
fafFO8 has female sterile | recessive phenotype, non-suppressible by DNApol-α60Sfaf240/DNAprim[+]
fafFO8 has female sterile | recessive phenotype, non-suppressible by S(faf)196196/S(faf)196[+]
fafFO8 has female sterile | recessive phenotype, non-suppressible by S(faf)244[+]/S(faf)244244
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
fafFO8/fafBX3 has eye phenotype, enhanceable by shi[+]/shiEM45
fafFO8/fafBX3 has eye phenotype, enhanceable by shi1/shi1
fafFO8/fafBX3 has eye phenotype, enhanceable by shi2/shi2
fafFO8/fafBX3 has eye phenotype, enhanceable by lqfAG/lqf[+]
fafFO8/fafBX3 has eye phenotype, enhanceable by Chc[+]/Chc1
fafFO8/fafBX3 has eye phenotype, enhanceable by shiEM42/shi[+]
NOT Enhanced by
Statement
Reference
fafFO8 has phenotype, non-enhanceable by Chc4
fafFO8/fafBX3 has ommatidium phenotype, non-enhanceable by arm1/arm[+]
fafFO8/fafBX3 has ommatidium phenotype, non-enhanceable by arm4/arm[+]
fafFO8/fafBX3 has ommatidium phenotype, non-enhanceable by cno3/cno[+]
fafFO8/fafBX3 has ommatidium phenotype, non-enhanceable by cno2/cno[+]
Suppressed by
Statement
Reference
fafFO8/fafBX4 has eye phenotype, suppressible by lqf+t19G
fafFO8/fafBX4 has eye phenotype, suppressible by lqfro.Tag:FLAG
fafFO8/fafBX3, lqfbE25 has ommatidium phenotype, suppressible by effXS347
fafFO8/fafBX3, lqfbE25 has ommatidium phenotype, suppressible by eff01462
fafFO8/fafBX3, lqfbE25 has ommatidium phenotype, suppressible by Prosβ61
fafFO8/fafBX4 has ommatidium phenotype, suppressible by Mmus\Usp9x+.ro
fafFO8/fafBX4 has ommatidium phenotype, suppressible by Mmus\Usp9x-.ro
fafFO8 has ommatidium phenotype, suppressible by S(faf)244[+]/S(faf)244244
fafFO8 has ommatidium phenotype, suppressible by S(faf)8686/S(faf)86[+]
fafFO8 has ommatidium phenotype, suppressible by eff[+]/effSfaf184
fafFO8 has ommatidium phenotype, suppressible by DNAprim[+]/DNApol-α60j10B2
fafFO8 has ommatidium phenotype, suppressible by Df(3L)rdgC-co2/+
fafFO8 has ommatidium phenotype, suppressible by DNApol-α60Sfaf240/DNAprim[+]
fafFO8 has ommatidium phenotype, suppressible by S(faf)196196/S(faf)196[+]
fafFO8 has eye phenotype, suppressible by Scer\CDC34ro.Tag:MYC
fafFO8 has eye phenotype, suppressible by Scer\UBP2ro.hs.Tag:MYC
fafFO8 has eye phenotype, suppressible by effS98
fafFO8 has eye phenotype, suppressible by effXS347
fafFO8 has eye phenotype, suppressible by Prosβ61
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
fafFO8 is an enhancer of eye photoreceptor cell phenotype of Rap1E8
fafFO8 is an enhancer of eye photoreceptor cell phenotype of Rap1E127
NOT Enhancer of
Statement
Reference
fafFO8/faf[+] is a non-enhancer of ommatidium phenotype of cnomis1
fafFO8/faf[+] is a non-enhancer of ommatidium phenotype of cnomis1/cno2
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
fafFO8/fafBX4 is a non-suppressor of synapse phenotype of hiwunspecified
Other
Additional Comments
Genetic Interactions
Statement
Reference
Embryos derived from fafFO8/+ females mated to dpphr4/+ males show strong lethality (only 10% of the expected number survive). The embryos have a ventralised cuticle phenotype. Embryos derived from Med8/+ females mated to males heterozygous for fafFO8 show semi-lethality. fafFO8/+ partially suppresses the cuticle phenotype of sogY506 hemizygous embryos. The embryos have partially restored denticles, a normal body shape and poorly developed Filzkorper. The survival of the progeny of fafFO8/+ females mated to dpphr4/+ males is increased 1.6-fold by basal expression of MedScer\UAS.P\T.cSa (in the absence of a Scer\GAL4 driver) in the females, while basal expression of MedK738R.Scer\UAS.P\T in the females increases survival of the progeny 4.2-fold. Expression of either MedScer\UAS.P\T.cSa or MedK738R.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the females increases survival of the progeny to approximately 80% in both cases. The ventralised cuticle phenotype of embryos derived from fafFO8/+ females mated to dpphr4/+ males is not rescued by basal expression of MedScer\UAS.P\T.cSa (in the absence of a Scer\GAL4 driver) in the females, while basal expression of MedK738R.Scer\UAS.P\T in the females results in the rescue of the ventralised cuticle phenotype in some embryos. Expression of either MedScer\UAS.P\T.cSa or MedK738R.Scer\UAS.P\T under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 in the females effectively rescues the ventralised cuticle phenotype of the embryos.
The addition of either homozygous fafBX4, or fafBX4/fafFO8 suppresses the physiological phenotype of hiwunspecified, leading to a more tha doubling of both quantal content and mEJP frequency. However the synaptic overgrowth phenotype is not affected.
The eyes of fafBX3/fafFO8 flies are severely malformed if the flies are also carrying one copy of lqfAG. The fafBX3/fafFO8 phenotype is dominantly enhanced by Chc1, no enhancement if seen with Chc4. The fafBX3/fafFO8 phenotype is dominantly enhanced by shiEM42 or shiEM45. The fafBX3/fafFO8 phenotype is slightly enhanced if the flies are also homozygous for shi1 or shi2. The lqfbE25 fafBX3/fafFO8 mutant phenotype is suppressed by effXS347, eff01462, Pros26rv10e or Pros261. The fafFO8/fafBX4 eye phenotype is completely suppressed by one copy of lqf+t19G or lqfro.T:Zzzz\FLAG.
The addition of effXS347 or effS98 dominantly suppresses the fafFO8 eye phenotype. The addition of three copies of P{ro-UbcD1.T:Hsap\MYC} to fafFO8/fafBX3 flies enhances the eye phenotype.
Heterozygosity for Pros261 dramatically reduces the severity of the eye phenotype of fafFO8 homozygotes.
Xenogenetic Interactions
Statement
Reference
Mmus\Usp9x+.ro or Mmus\Usp9x-.ro rescue nearly completely the eye phenotype of fafBX4/fafFO8 flies. Embryos derived from fafFO8 females which are expressing Mmus\Usp9x+.Scer\UAS.P\T in the germline under the control of Scer\GAL4nos.UTR.T:Hsim\VP16 survive to adulthood and appear to be normal, fertile adults.
The addition of Scer\UBP2ro.hs.T:Hsap\MYC or Scer\cdc34ro.T:Hsap\MYC but not Scer\DOA4ro.T:Hsap\MYC rescues the homozygous fafFO8 eye phenotype.
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Fischer-Vize, Carthew and Rubin.
Comments
Comments
Very strong faf allele.
Strong allele.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (18)