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General Information
Symbol
Dmel\grk2B6
Species
D. melanogaster
Name
FlyBase ID
FBal0031404
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
grk2B
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

A 442 bp deletion is reported in FBrf0127360; due to differences between the reference sequence and the sequence reported in GB:AF223394, the endpoints of the 442 bp deletion in GB:AF223394 map to a slightly longer fragment in the reference sequence

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

442bp deletion starting at nucleotide 236.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eggs derived from grk2B6/grkHF females lack operculum regions II and III (the section that normally extends from the area surrounding the micropyle to the dorsal appendages and the section that is normally between the dorsal appendages), and instead only region I cells are present symmetrically surrounding the micropyle, following a symmetric collar that separates the operculum from the rest of the chorion.

Stage 12 grk3/grk2B6 mutant egg chambers lack dorsal appendages.

In egg chambers with grk2B6 germline clones, the oocyte nucleus is incorrectly located at the posterior of the oocyte in 94/154 cases observed. In egg chambers with a mosaic germline composed of eight grk2B6 nurse cells, seven wild-type nurse cells and a wild-type oocyte, the oocyte nucleus is properly localized at the anterior margin of the oocyte in all observed cases. Likewise, the oocyte is also anteriorly localized when egg chambers have a grk2B6 oocyte with eight grk2B6 nurse cells and seven wild-type nurse cells. In germline mosaics with a grk2B6 oocyte, the border cell cluster is correctly dorsally localized and dorsal follicle cell fates are not affected.

Heterozygous females lay eggs with fused or partially fused dorsal appendages (75% at 18oC, 27.7% at 25oC and 13.8% at 29oC).

In grk2B6/grk1 oocytes the anterior-posterior gradient of microtubule concentration seen in wild-type is abolished. However, concentration of microtubules around the mis-localized nucleus persists.

Ovaries from grk1/grk2B6 mutant females show a range of defects. In mildly affected egg chambers where the germinal vesicle has moved anterior and dorsal, border cells complete posterior migration but fail to migrate dorsally.

Embryos laid by homozygous grk2B6 mothers are strongly ventralised.

Microtubule cytoskeleton in grk2B6/grk1 oocytes is defective due to premature streaming of the microtubules.

grk2B6/grk2E12 egg chambers show a duplication of all three anterior follicle cell types at the posterior pole.

grk2B6/grk2 heterozygous eggs lack dorsal ventral polarity and possess a posterior micropyle. The eggs never contain embryos.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Suppressor of
Statement
Reference
NOT Suppressor of
Phenotype Manifest In
Enhanced by
Statement
Reference

grk2B6 has dorsal appendage phenotype, enhanceable by CSN5[+]/CSN5ex15

grk2B6 has dorsal appendage phenotype, enhanceable by CSN5ex35/CSN5[+]

grk2B6 has dorsal appendage phenotype, enhanceable by CSN5[+]/CSN5ex9

grk2B6 has dorsal appendage phenotype, enhanceable by CSN5L4032/CSN5[+]

NOT Enhanced by
Statement
Reference

grk2B6 has dorsal appendage phenotype, non-enhanceable by CSN5ex27/CSN5[+]

Suppressed by
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

grk3, grk3, grk2B6 is a non-suppressor of dorsal appendage phenotype of cicfet-U6/cicfet-T6

grk3, grk3, grk2B6 is a non-suppressor of egg phenotype of cicfet-U6/cicfet-T6

Other
Additional Comments
Genetic Interactions
Statement
Reference

grk3/grk2B6 suppresses the over proliferation of posterior follicle cells in Dis3G5039/Dis32 egg chambers.

Eggs derived from females expressing dppScer\UAS.cSa under the control of Scer\GAL4CY2 in a grk2B6/grkHF background have misshaped micropyles, show the loss of the collar that normally separates the operculum from the rest of the chorion and show a reduction in egg diameter.

Stage 12 grk3/grk2B6 ; cicfet-U6/cicfet-T6 double mutant egg chambers lack dorsal appendages (characteristic of loss of grk), but retain a pronounced collar of appendage material, indicating that loss of grk cannot suppress the cic phenotype.

Expression of grk::ylgrk1-275.yl1826-1984 is able to suppress the grk2B6/grkHF oogenesis phenotype.

grk::ylgrk1-275.yl1826-1984.

Heterozygosity for grk2B6 enhances the penetrance of dorsal appendage loss or fusion in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa females, from 5% to 90%. This phenotype still shows over 90% penetrance in eggs laid by Scer\GAL4nos.UTR.T:Hsim\VP16; aretScer\UAS.P\T.cFa; grk2B6/+; vasαTub67C.T:Avic\GFP flies.

The penetrance and expressivity of the weak ventralization phenotype seen in eggs laid by kek1Scer\UAS.P\T.cGa; Scer\GAL4nos.UTR.T:Hsim\VP16 females is strongly enhanced by heterozygosity for grk2B6.

The fused dorsal appendage phenotype of eggs laid by grk2B6/+ females is enhanced if the females are also heterozygous for CSN5L4032, CSN5ex15, CSN5ex9 or CSN5ex35 but is not enhanced if they are also heterozygous for CSN5ex27.

Xenogenetic Interactions
Statement
Reference

The presence of one or two copies of Dwil\GK18382+ta in grk2B6/grk2E12 mutant D. melanogaster flies results in four distinct eggshell phenotypes. Most eggshells, 69% and 78% from one and two copies of Dwil\GK18382+ta, respectively, are wild-type or wild-type with an enlarger area posterior to the base of the dorsal appendages, similar to wild-type flies expressing Dwil\GK18382+ta. A dorsal ridge-like morphology is found in 10% and 1% of the eggshells from one and two copies of Dwil\GK18382+ta, respectively.

In 13% and 18% of the eggshells from in grk2B6/grk2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382+ta, respectively, there is a single or fused dorsal appendage.

In 8% and 3% of the eggshells from in grk2B6/grk2E12 mutant D. melanogaster with one and two copies of Dwil\GK18382+ta, respectively, the eggshells are collapsed and the eggs unmountable.

Complementation and Rescue Data
Partially rescued by

grk2B6 is partially rescued by grkΔC

grk2B6 is partially rescued by grkt5

Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (53)