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General Information
Symbol
Dmel\hhbar3
Species
D. melanogaster
Name
FlyBase ID
FBal0031487
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hh1, bar-3
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

1885bp deletion within a hh intron. The coordinates of the hh deletion are bases 6053-7938 relative to the location of the P{PZ}hhP30 insertion site.

Deletion of non-coding sequences within the first intron.

1.8kb deletion of sequences between +6.0 and +7.8kb. 1.8kb deletion of sequences from position +6.0 to +7.8.

Associated with a deficiency of approximately 2kb.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

lamina & neuron

photoreceptor cell & axon

photoreceptor cell R7 & axon

photoreceptor cell R8 & axon

Detailed Description
Statement
Reference

Most hhbar3/hhbar3 mutant adults exhibit a small reduction in the area between wing veins L3 and L4, and a small minority exhibit wing blisters, as compared to controls.

hh2/hhbar3 mutant adults exhibit smaller wings with an ectopic crossvein, as compared to controls.

hhbar3 mutant eye diss exhibit ommatidial developmental defects.

The eyes of hh8/hhbar3 flies are smaller than wild-type and are slightly rough, due to some disorganisation of ommatidial and inter-ommatidial structure.

Homozygotes have a rough eye which has an indented anterior side.

hhbar3/hh8 flies have a slight rough eye phenotype.

hhbar3 heterozygotes are not significantly different from wild-type.

hhbar3 is a strong, eye-specific hypomorph. It is fully recessive in trans to wild-type, but has a severely reduced eye when homozygous (68% smaller than hhbar3/+) and in trans to the null hhAC it is smaller still (82% smaller than hhbar3/+). This suggests that hhbar3 is not an amorph for eye size by Muller's test: the phenotype becomes stronger in trans to the null.

hhbar3 homozygotes have about 10 columns of ommatidia compared to 28 to 30 in wild-type.

hhbar3/hhAC mutants exhibit about six columns of ommatidia per eye compared to 28-30 columns in wild-type.

R7 and R8 photoreceptor cell growth cones still form two layers in the medulla in hhbar3 animals, although they remain closely associated during the early pupal stage (17% APF).

dac-positive lamina neurons fail to differentiate in hhbar3 mutants.

The morphogenetic furrow becomes arrested in the eye discs of third instar mutant larvae.

The compound eyes of mutant animals lack anterior structures.

Flies homozygous for hhbar3 have narrow kidney-shaped eyes.

In hhbar3 mutant eye discs, most of the ommatidial clusters over-rotate, with 33.7% of them reaching 110o, compared to only 1.4% in wild-type discs (most ommatidial rotation in wild-type eye discs of late third larval and early pupal stages is about 50o-60o). The overrotation defect in these mutant discs correlated with the disappearance of the morphogenetic furrow (MF): In late third instar mutant eye discs the MF is still present, and most ommatidial clusters rotate to the normal position of 50o-60o, apart from a few in the most posterior region that over-rotate. As development progresses the MF becomes increasingly disrupted, and the over-rotation phenotype becomes more prominent. By the time MF progression is completely disrupted most of the ommatidial clusters have over-rotated, with many of them reaching 110o-120o. This overrotation phenotype persists to the adult stage, where 47.3% of ommatidia have a rotation degree larger than 105o, compared to 1.1% in wild-type.

In hhbar3 mutant larvae the posterior eye field develops normally, but anterior progression of the morphogenetic furrow is inhibited.

A small number of lamina neurons develop compared to wild type in hhbar3 animals. The development of glial cells is not affected. R1-R6 axons stop in the lamina (as in wild type) in these animals. The array of R7 and R8 growth cones in the medulla is indistinguishable from wild type.

Defective spatial positioning of the R7,8 axons is seen in 18% of hhbar3 homozygotes. Sometimes in just one lobe, and others in both. In the cases where both are affected the direction of the misorientation is the same.

Heterozygotes show a quantitative effect on wing shape in intervein regions C and D compared to wild type.

The eye is reduced in homozygotes, containing approximately 13 vertical rows of ommatidia from the posterior end.

Homozygotes have extremely reduced eyes, which contain approximately 150 (rather than the normal 800) ommatidia.

The eye discs contain about 11 columns of ommatidial R cell clusters. The most anterior clusters appear normal and the axon fascicles from them are found in their proper dorsoventral positions posterior to the lamina furrow. But viewed on their antero-posterior axis the fascicles are collapsed on each other. Glial cells are absent from the optic stalk and lamina. Lamina precursor cells fail to undergo their terminal division, arresting in G1 at the lamina furrow.

Homozygotes lack portions of the anterior eye, due to a defect in morphogenetic furrow progression. Eye phenotype is suppresses when in combination with Pka-C1DN heterozygote, cell death is also suppressed.

Arrests morphogenetic furrow movement after differentiation of 8-10 rows of photoreceptors. In wgl-12; hhbar3 eye discs the hhbar3 mutation completely abolishes the effects of loss of wg expression (inwardly directed furrow movement is not present).

An eye specific hh allele. Double mutants of bunrI043 and hhbar3 all die as pupae.

Adult eye is reduced in size. hhbar3/hh6 produces an intermediate reduced eye phenotype.

When in combination with Df(2R)enE/en1 a phenotype reminiscent of a weak dpp mutation is produced, narrowing of the space between wing veins 3 and 4. Posterior wing clones affect adjacent cells of the anterior compartment and cause an overall reduction in wing size.

The progression of the morphogenetic furrow in the developing eye disc arrests. The eye disc appears normal at the time of arrest. dpp expression is abolished (as assayed with a dpp-lacZ fusion gene).

Slight increase in bristles of all tarsal rows in the second leg.

Transheterozygotes with hhAC display an eye phenotype at 25oC.

Adult viable allele.

A weak hypomorphic allele that is not complemented by other hh alleles. Eye of homozygote small and narrow with about 150 facets. Eye disc size reduced; deep cleft at anterior edge cell; clusters at cleft look mature (Renfranz and Benzer, 1989). viable

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

hhbar3 has size defective phenotype, enhanceable by apt167/apt[+]

hhbar3/hh8 has visible phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has visible phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has visible phenotype, enhanceable by snama1/mnm[+]

Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference

hhbar3/hh8 has visible phenotype, non-suppressible by BRWD3s5349/BRWD3[+]

Enhancer of
Statement
Reference

hhbar3/hhbar3 is an enhancer of visible | dominant phenotype of L2

hh[+]/hhbar3 is an enhancer of planar polarity defective phenotype of scaBP1/scaBP2

NOT Suppressor of
Statement
Reference

hh[+]/hhbar3 is a non-suppressor of size defective phenotype of Lfee

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

hhbar3 has wing phenotype, enhanceable by apt167/apt[+]

hhbar3/hh8 has eye phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has ommatidium phenotype, enhanceable by snamaPX1/mnm[+]

hhbar3/hh8 has eye phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has ommatidium phenotype, enhanceable by l(2)rQ313[+]/snamarQ313

hhbar3/hh8 has eye phenotype, enhanceable by snama1/mnm[+]

hhbar3/hh8 has ommatidium phenotype, enhanceable by snama1/mnm[+]

hhbar3 has eye | anterior phenotype, enhanceable by foi[+]/foiB1-089

hhbar3 has eye phenotype, enhanceable by oro1/oro[+]

hhbar3 has ommatidium phenotype, enhanceable by oro1/oro[+]

hhbar3 has eye phenotype, enhanceable by oro2/oro[+]

hhbar3 has ommatidium phenotype, enhanceable by oro2/oro[+]

hhbar3 has ommatidium phenotype, enhanceable by Ten-m[+]/Ten-m05309

hhbar3 has ommatidium phenotype, enhanceable by Df(3L)Ten-m-AL3/+

Suppressed by
Statement
Reference

hhbar3/hh8 has eye phenotype, suppressible by BRWD3ram1

hhbar3/hh8 has ommatidium phenotype, suppressible by BRWD3ram1

hhbar3 has eye phenotype, suppressible by trr+t12

hhbar3 has eye phenotype, suppressible | partially by ptc14/ptctuf-1

hhbar3 has eye phenotype, suppressible | partially by ptc16/ptctuf-1

hhbar3 has eye phenotype, suppressible | partially by ptc34/ptctuf-1

hhbar3 has eye phenotype, suppressible | partially by ptc37/ptctuf-1

hhbar3 has eye phenotype, suppressible | partially by ptc47/ptctuf-1

hhbar3 has eye phenotype, suppressible by ptc13/ptctuf-1

hhbar3 has macrochaeta | ectopic phenotype, suppressible | partially by scM6

hhbar3 has phenotype, suppressible | partially by amosRoi-1

NOT suppressed by
Statement
Reference

hhbar3 has macrochaeta | ectopic phenotype, non-suppressible by scsisB-2

hhbar3 has macrochaeta | ectopic phenotype, non-suppressible by scsisB-3

Enhancer of
Statement
Reference

hhbar3/hhbar3 is an enhancer of eye phenotype of L2

hh[+]/hhbar3 is an enhancer of ommatidium phenotype of scaBP1/scaBP2

Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference

hh[+]/hhbar3 is a non-suppressor of eye phenotype of Lfee

hhbar3/hhbar3 is a non-suppressor of ommatidium phenotype of nmoj147-1/nmoP1

Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

The presence of the apt167/+ mutation enhances the severity of the wing defects seen in hhbar3/hhbar3 mutant adults, showing further reduced wing area between L3 and L4, small wings and blistering.

A subset of CycE2/+, hhbar3/hhbar3 double mutant flies show smaller, blistered wings, as compared to controls.

The reduced eye size of Lfee flies is not restored by hhbar3/+.

BRWD3ram1 suppresses the small, rough eye phenotype of hh8/hhbar3 flies.

The presense of BRWD3s5349/+ does not significantly affect the size or roughness of eye in hh8/hhbar3 flies. However, retinal sections reveal the presence of small rhabdomeres in these eyes, which are not present in the eyes of hh8/hhbar3 animals in the absence of BRWD3s5349/+.

L2/+ ; hhbar3/hhbar3 flies show complete elimination of the eye.

When eyScer\UAS.cHa clones (driven by Scer\GAL4Act5C.PI) are made in the wing disc in a hhbar3 background no ectopic eyes are seen.

The hhbar3 eye phenotype is suppressed by ptc13/ptctuf-1 and partially suppressed by ptctuf-1 in trans to ptc16, ptc14, ptc34, ptc37 or ptc47.

Ectopic macrochaetae on the scutellum of hhbar3 homozygotes is suppressed by 75% in scM6 hemizygotes, but is only very weakly suppressed by hemizygous scsisB-1. This phenotype is not suppressed by hemizygous scsisB-2 or scsisB-3.

The over-rotation of ommatidia seen in hhbar3 mutant eye discs and adult eyes, is suppressed by scaScer\UAS.cEa; Scer\GAL4hs.2sev.

The reduced eye phenotype is dominantly enhanced by oro1 and oro2. The eyes of oro1/+ ; hhbar3/hhbar3 flies contain approximately 9 rows vertical rows of ommatidia from the posterior end. The eyes of ptc9/+ ; hhbar3/hhbar3 and ptc9/oro1 ; hhbar3/hhbar3 flies are similar in size, suggesting that ptc is epistatic to oro with respect to effects on hhbar3.

Df(3L)Ten-m-AL3/+ and Ten-m05309/+ each enhance the loss of ommatidia phenotype which is seen in hhbar3 homozygotes.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (5)
Notes on Origin
Discoverer
Comments
Comments

Eye specific allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (54)