Allele Dmel\hh^bar3

General Information
Symbol	Dmel\hh^bar3	Species	D. melanogaster
Name		FlyBase ID	FBal0031487
Feature type	allele	Associated gene	Dmel\hh

Allele class	hypomorphic allele - genetic evidence
Mutagen	spontaneous
Recent Updates
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FB2012_01	References Levine et al., 1997, Dev. Dynamics 209(1): 1--14
FB2011_10
All updates	Click here to see a list of all updates to this record from FB2010_08 and on.
Nature of the Allele
Allele class	hypomorphic allele - genetic evidence (Lee et al., 1992, Epps et al., 1997, Chotard et al., 2005, )
Mutagen	spontaneous
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference hh^bar3 contains a 1885 nucleotide deletion, from position 6053 to 7938 (bases numbered from the site of P30). (Rogers et al., 2005) Deletion of non-coding sequences within the first intron. (Chiang and Beachy, 1994) 1.8kb deletion of sequences between +6.0 and +7.8kb. 1.8kb deletion of sequences from position +6.0 to +7.8. (Lee et al., 1992) Associated with a deficiency of approximately 2kb. (Mohler and Vani, 1992)
Cytology
Phenotypic Data
Phenotypic Class
	planar polarity defective \| recessive (Chou and Chien, 2002) viable (Lee et al., 1992, Treisman et al., 1995, Rogers et al., 2005) visible (with hh⁸) (Jones et al., 2006, D'Costa et al., 2006) visible \| recessive (Mohler, 1988, Held, 1993, Lee et al., 1992, Jones et al., 2006)
Phenotype Manifest In
	1st posterior cell (Palsson and Gibson, 2000) 2nd posterior cell (Palsson and Gibson, 2000) embryonic/larval optic stalk (Huang and Kunes, 1996) eye (Epps et al., 1997, Pan and Rubin, 1995, Sedkov et al., 2003, Chiang and Beachy, 1994, Jones et al., 2006, Rogers et al., 2005, Tokhunts et al., 2010) eye (with hh⁸) (D'Costa et al., 2006) eye \| anterior (Pielage et al., 2004) glial cell (Huang and Kunes, 1996) lamina (Huang and Kunes, 1996) lamina & neuron (Poeck et al., 2001) lamina forming neuroblast (Huang and Kunes, 1996) lamina intrinsic neuron (Yoshida et al., 2005) lamina tangential neuron (Yoshida et al., 2005) mesothoracic tarsal bristle longitudinal row 1 (Held, 1993) mesothoracic tarsal bristle longitudinal row 2 (Held, 1993) mesothoracic tarsal bristle longitudinal row 2.5 (Held, 1993) mesothoracic tarsal bristle longitudinal row 3 (Held, 1993) mesothoracic tarsal bristle longitudinal row 3.5 (Held, 1993) mesothoracic tarsal bristle longitudinal row 4 (Held, 1993) mesothoracic tarsal bristle longitudinal row 5 (Held, 1993) mesothoracic tarsal bristle longitudinal row 5.5 (Held, 1993) mesothoracic tarsal bristle longitudinal row 6 (Held, 1993) mesothoracic tarsal bristle longitudinal row 6.5 (Held, 1993) mesothoracic tarsal bristle longitudinal row 7 (Held, 1993) mesothoracic tarsal bristle longitudinal row 8 (Held, 1993) morphogenetic furrow (Chou and Chien, 2002, Huang and Kunes, 1996, Hummel et al., 2002, Lim and Choi, 2004, Treisman and Rubin, 1995) ommatidial precursor cluster (Rogers et al., 2005) ommatidial precursor cluster (with hh^AC) (Rogers et al., 2005) ommatidium (Epps et al., 1997, Chou and Chien, 2002, Jones et al., 2006, Tokhunts et al., 2010, Levine et al., 1997) ommatidium (with hh⁸) (Jones et al., 2006, D'Costa et al., 2006) photoreceptor cell & axon (Huang and Kunes, 1996) photoreceptor cell R7 & axon (Hoyle et al., 2000) photoreceptor cell R8 & axon (Hoyle et al., 2000)
Detailed Description
	Statement Reference hh[bar3] mutant eye diss exhibit ommatidial developmental defects. (Tokhunts et al., 2010) The eyes of hh⁸/hh^bar3 flies are smaller than wild-type and are slightly rough, due to some disorganisation of ommatidial and inter-ommatidial structure. (D'Costa et al., 2006) Homozygotes have a rough eye which has an indented anterior side. hh^bar3/hh⁸ flies have a slight rough eye phenotype. (Jones et al., 2006) hh^bar3 heterozygotes are not significantly different from wild-type. hh^bar3 is a strong, eye-specific hypomorph. It is fully recessive in trans to wild-type, but has a severely reduced eye when homozygous (68% smaller than hh^bar3/+) and in trans to the null hh^AC it is smaller still (82% smaller than hh^bar3/+). This suggests that hh^bar3 is not an amorph for eye size by Muller's test: the phenotype becomes stronger in trans to the null. hh^bar3 homozygotes have about 10 columns of ommatidia compared to 28 to 30 in wild-type. hh^bar3/hh^AC mutants exhibit about six columns of ommatidia per eye compared to 28-30 columns in wild-type. (Rogers et al., 2005) R7 and R8 photoreceptor cell growth cones still form two layers in the medulla in hh^bar3 animals, although they remain closely associated during the early pupal stage (17% APF). (Ting et al., 2005) dac-positive lamina neurons fail to differentiate in hh^bar3 mutants. (Yoshida et al., 2005) The morphogenetic furrow becomes arrested in the eye discs of third instar mutant larvae. (Lim and Choi, 2004) The compound eyes of mutant animals lack anterior structures. (Pielage et al., 2004) Flies homozygous for hh^bar3 have narrow kidney-shaped eyes. (Thomas and Ingham, 2003) In hh^bar3 mutant eye discs, most of the ommatidial clusters over-rotate, with 33.7% of them reaching 110^o, compared to only 1.4% in wild-type discs (most ommatidial rotation in wild-type eye discs of late third larval and early pupal stages is about 50^o-60^o). The overrotation defect in these mutant discs correlated with the disappearance of the morphogenetic furrow (MF): In late third instar mutant eye discs the MF is still present, and most ommatidial clusters rotate to the normal position of 50^o-60^o, apart from a few in the most posterior region that over-rotate. As development progresses the MF becomes increasingly disrupted, and the over-rotation phenotype becomes more prominent. By the time MF progression is completely disrupted most of the ommatidial clusters have over-rotated, with many of them reaching 110^o-120^o. This overrotation phenotype persists to the adult stage, where 47.3% of ommatidia have a rotation degree larger than 105^o, compared to 1.1% in wild-type. (Chou and Chien, 2002) In hh^bar3 mutant larvae the posterior eye field develops normally, but anterior progression of the morphogenetic furrow is inhibited. (Hummel et al., 2002) A small number of lamina neurons develop compared to wild type in hh^bar3 animals. The development of glial cells is not affected. R1-R6 axons stop in the lamina (as in wild type) in these animals. The array of R7 and R8 growth cones in the medulla is indistinguishable from wild type. (Poeck et al., 2001) Defective spatial positioning of the R7,8 axons is seen in 18% of hh^bar3 homozygotes. Sometimes in just one lobe, and others in both. In the cases where both are affected the direction of the misorientation is the same. (Hoyle et al., 2000) Heterozygotes show a quantitative effect on wing shape in intervein regions C and D compared to wild type. (Palsson and Gibson, 2000) The eye is reduced in homozygotes, containing approximately 13 vertical rows of ommatidia from the posterior end. (Epps et al., 1997) Homozygotes have extremely reduced eyes, which contain approximately 150 (rather than the normal 800) ommatidia. (Levine et al., 1997) The eye discs contain about 11 columns of ommatidial R cell clusters. The most anterior clusters appear normal and the axon fascicles from them are found in their proper dorsoventral positions posterior to the lamina furrow. But viewed on their antero-posterior axis the fascicles are collapsed on each other. Glial cells are absent from the optic stalk and lamina. Lamina precursor cells fail to undergo their terminal division, arresting in G₁ at the lamina furrow. (Huang and Kunes, 1996) Homozygotes lack portions of the anterior eye, due to a defect in morphogenetic furrow progression. Eye phenotype is suppresses when in combination with Pka-C1^DN heterozygote, cell death is also suppressed. (Pan and Rubin, 1995) Arrests morphogenetic furrow movement after differentiation of 8-10 rows of photoreceptors. In wg^l-12; hh^bar3 eye discs the hh^bar3 mutation completely abolishes the effects of loss of wg expression (inwardly directed furrow movement is not present). (Treisman and Rubin, 1995) An eye specific hh allele. Double mutants of bun^rI043 and hh^bar3 all die as pupae. (Treisman et al., 1995) Adult eye is reduced in size. hh^bar3/hh⁶ produces an intermediate reduced eye phenotype. (Chiang and Beachy, 1994) When in combination with Df(2R)en^E/en¹ a phenotype reminiscent of a weak dpp mutation is produced, narrowing of the space between wing veins 3 and 4. Posterior wing clones affect adjacent cells of the anterior compartment and cause an overall reduction in wing size. (Hidalgo, 1994) The progression of the morphogenetic furrow in the developing eye disc arrests. The eye disc appears normal at the time of arrest. dpp expression is abolished (as assayed with a dpp-lacZ fusion gene). (Heberlein et al., 1993) Slight increase in bristles of all tarsal rows in the second leg. (Held, 1993) Transheterozygotes with hh^AC display an eye phenotype at 25^oC. (Lee et al., 1992) Adult viable allele. (Mohler, 1988, Mohler and Vani, 1992) A weak hypomorphic allele that is not complemented by other hh alleles. Eye of homozygote small and narrow with about 150 facets. Eye disc size reduced; deep cleft at anterior edge cell; clusters at cleft look mature (Renfranz and Benzer, 1989). viable
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference hh^bar3/hh⁸ has visible phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313 (Jones et al., 2006) hh^bar3/hh⁸ has visible phenotype, enhanceable by snama¹/mnm[+] (Jones et al., 2006) hh^bar3/hh⁸ has visible phenotype, enhanceable by snama^PX1/mnm[+] (Jones et al., 2006)
Suppressed by
Statement Reference hh^bar3/hh⁸ has visible phenotype, suppressible by BRWD3^ram1 (D'Costa et al., 2006) hh^bar3 has planar polarity defective \| recessive phenotype, suppressible by sca^Scer\UAS.cEa/Scer\GAL4^hs.2sev (Chou and Chien, 2002)
NOT suppressed by
Statement Reference hh^bar3/hh⁸ has visible phenotype, non-suppressible by BRWD3^s5349/BRWD3[+] (D'Costa et al., 2006)
Enhancer of
Statement Reference hh[+]/hh^bar3 is an enhancer of planar polarity defective phenotype of sca^BP1/sca^BP2 (Chou and Chien, 2002) hh^bar3/hh^bar3 is an enhancer of visible \| dominant phenotype of L² (Singh et al., 2005, Singh et al., 2005)
NOT Suppressor of
Statement Reference hh[+]/hh^bar3 is a non-suppressor of size defective phenotype of L^fee (Wang and Huang, 2009) hh^bar3/hh^bar3 is a non-suppressor of planar polarity defective phenotype of nmo^j147-1/nmo^P1 (Chou and Chien, 2002, Chou and Chien, 2002)
Phenotype Manifest In
Enhanced by
Statement Reference hh^bar3/hh⁸ has eye phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313 (Jones et al., 2006) hh^bar3/hh⁸ has eye phenotype, enhanceable by snama¹/mnm[+] (Jones et al., 2006) hh^bar3/hh⁸ has eye phenotype, enhanceable by snama^PX1/mnm[+] (Jones et al., 2006) hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by l(2)rQ313[+]/snama^rQ313 (Jones et al., 2006) hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by snama¹/mnm[+] (Jones et al., 2006) hh^bar3/hh⁸ has ommatidium phenotype, enhanceable by snama^PX1/mnm[+] (Jones et al., 2006) hh^bar3 has eye \| anterior phenotype, enhanceable by foi[+]/foi^B1-089 (Pielage et al., 2004) hh^bar3 has eye phenotype, enhanceable by oro¹/oro[+] (Epps et al., 1997) hh^bar3 has eye phenotype, enhanceable by oro²/oro[+] (Epps et al., 1997) hh^bar3 has ommatidium phenotype, enhanceable by Df(3L)Ten-m-AL3/+ (Levine et al., 1997) hh^bar3 has ommatidium phenotype, enhanceable by oro¹/oro[+] (Epps et al., 1997) hh^bar3 has ommatidium phenotype, enhanceable by oro²/oro[+] (Epps et al., 1997) hh^bar3 has ommatidium phenotype, enhanceable by Ten-m[+]/Ten-m⁰⁵³⁰⁹ (Levine et al., 1997)
Suppressed by
Statement Reference hh^bar3/hh⁸ has eye phenotype, suppressible by BRWD3^ram1 (D'Costa et al., 2006) hh^bar3/hh⁸ has ommatidium phenotype, suppressible by BRWD3^ram1 (D'Costa et al., 2006) hh^bar3 has eye phenotype, suppressible \| partially by ptc¹⁴/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible \| partially by ptc¹⁶/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible \| partially by ptc³⁴/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible \| partially by ptc³⁷/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible \| partially by ptc⁴⁷/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible by ptc¹³/ptc^tuf-1 (Thomas and Ingham, 2003) hh^bar3 has eye phenotype, suppressible by trr^+t12 (Sedkov et al., 2003) hh^bar3 has macrochaeta \| ectopic phenotype, suppressible \| partially by sc^M6 (Wrischnik et al., 2003) hh^bar3 has macrochaeta \| ectopic phenotype, suppressible \| partially by sc^sisB-1 (Wrischnik et al., 2003) hh^bar3 has ommatidium phenotype, suppressible by sca^Scer\UAS.cEa/Scer\GAL4^hs.2sev (Chou and Chien, 2002) hh^bar3 has phenotype, suppressible \| partially by amos^Roi-1 (Heberlein et al., 1993)
NOT suppressed by
Statement Reference hh^bar3 has macrochaeta \| ectopic phenotype, non-suppressible by sc^sisB-2 (Wrischnik et al., 2003) hh^bar3 has macrochaeta \| ectopic phenotype, non-suppressible by sc^sisB-3 (Wrischnik et al., 2003)
Enhancer of
Statement Reference hh[+]/hh^bar3 is an enhancer of ommatidium phenotype of sca^BP1/sca^BP2 (Chou and Chien, 2002) hh^bar3/hh^bar3 is an enhancer of eye phenotype of L² (Singh et al., 2005, Singh et al., 2005)
Suppressor of
Statement Reference hh^bar3 is a suppressor of eye \| ectopic \| somatic clone phenotype of Scer\GAL4^Act5C.PI/Scer\GAL4^Act5C.PI, ey^Scer\UAS.cHa (Kango-Singh et al., 2003)
NOT Suppressor of
Statement Reference hh[+]/hh^bar3 is a non-suppressor of eye phenotype of L^fee (Wang and Huang, 2009) hh^bar3/hh^bar3 is a non-suppressor of ommatidium phenotype of nmo^j147-1/nmo^P1 (Chou and Chien, 2002, Chou and Chien, 2002)
Other
Statement Reference BRWD3^s5349/BRWD3[+], hh^bar3/hh⁸ has rhabdomere phenotype (D'Costa et al., 2006)
Additional Comments
Genetic Interactions
Statement Reference The reduced eye size of L[fee] flies is not restored by hh[bar3]/+. (Wang and Huang, 2009) BRWD3^ram1 suppresses the small, rough eye phenotype of hh⁸/hh^bar3 flies. The presense of BRWD3^s5349/+ does not significantly affect the size or roughness of eye in hh⁸/hh^bar3 flies. However, retinal sections reveal the presence of small rhabdomeres in these eyes, which are not present in the eyes of hh⁸/hh^bar3 animals in the absence of BRWD3^s5349/+. (D'Costa et al., 2006) L²/+ ; hh^bar3/hh^bar3 flies show complete elimination of the eye. (Singh et al., 2005) When ey^Scer\UAS.cHa clones (driven by Scer\GAL4^Act5C.PI) are made in the wing disc in a hh^bar3 background no ectopic eyes are seen. (Kango-Singh et al., 2003) The hh^bar3 eye phenotype is suppressed by ptc¹³/ptc^tuf-1 and partially suppressed by ptc^tuf-1 in trans to ptc¹⁶, ptc¹⁴, ptc³⁴, ptc³⁷ or ptc⁴⁷. (Thomas and Ingham, 2003) Ectopic macrochaetae on the scutellum of hh^bar3 homozygotes is suppressed by 75% in sc^M6 hemizygotes, but is only very weakly suppressed by hemizygous sc^sisB-1. This phenotype is not suppressed by hemizygous sc^sisB-2 or sc^sisB-3. (Wrischnik et al., 2003) The over-rotation of ommatidia seen in hh^bar3 mutant eye discs and adult eyes, is suppressed by sca^Scer\UAS.cEa; Scer\GAL4^hs.2sev. (Chou and Chien, 2002) The reduced eye phenotype is dominantly enhanced by oro¹ and oro². The eyes of oro¹/+ ; hh^bar3/hh^bar3 flies contain approximately 9 rows vertical rows of ommatidia from the posterior end. The eyes of ptc⁹/+ ; hh^bar3/hh^bar3 and ptc⁹/oro¹ ; hh^bar3/hh^bar3 flies are similar in size, suggesting that ptc is epistatic to oro with respect to effects on hh^bar3. (Epps et al., 1997) Df(3L)Ten-m-AL3/+ and Ten-m[05309]/+ each enhance the loss of ommatidia phenotype which is seen in hh[bar3] homozygotes. (Levine et al., 1997)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescued by	hh^bar3 is rescued by hh^{N.Scer\UAS.T:Ivir\HA1}/Scer\GAL4^GMR.PF (Tokhunts et al., 2010) hh^bar3 is rescued by hh^Scer\UAS.cAa/Scer\GAL4^GMR.PF/Scer\GAL4^GMR.PF (Chou and Chien, 2002) hh^bar3 is rescued by hh^{Scer\UAS.T:Ivir\HA1}/Scer\GAL4^GMR.PF (Tokhunts et al., 2010) hh^bar3 is rescued by Scer\GAL4^GMR.PF/hh^U.Scer\UAS (Tokhunts et al., 2010)
Comments
Stocks ( 6 )
Bloomington	1376 hh^bar3 tx¹ 450 w¹¹¹⁸; hh^bar3/TM3, Sb¹ 1450 ru¹ h¹ red¹ cv-c¹ Sb^sbd-2 sr¹ e^s hh^bar3 tx¹ ca¹/TM6, h^*
Kyoto	105915 hh^bar3 106293 hh^bar3 tx¹ 106318 ru¹ h¹ red¹ cv-c¹ Sb^sbd-2 sr¹ e^s hh^bar3 tx¹ ca¹/TM6, h^*
Notes on Origin
Discoverer

Comments
	Eye specific allele. (Chiang and Beachy, 1994)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	bar-3 (Ma et al., 1996, Lee et al., 1992) hh1 (Hoyle et al., 2000) hh¹ (Singh et al., 2005, Pauli et al., 2005, Pielage et al., 2004, Ting et al., 2005, Lim and Choi, 2004, Dearborn and Kunes, 2004, Wrischnik et al., 2003, Thomas and Ingham, 2003, Sedkov et al., 2003, Tayler and Garrity, 2003, Chou and Chien, 2002, Kango-Singh et al., 2003, Hummel et al., 2002, Poeck et al., 2001, Palsson and Gibson, 2000, Chanut et al., 2000, Liu and Lengyel, 2000, Dominguez, 1999, Huang and Kunes, 1998, Epps et al., 1997, Treisman et al., 1995, Chotard et al., 2005, Rogers et al., 2005, Levine et al., 1997, Wang et al., 2008, Wang and Huang, 2009, Tokhunts et al., 2010) hh^bar-3 (Held, 1993) hh^bar3 (Jones et al., 2006, Rogers et al., 2005)
Name Synonym
Secondary FlyBase IDs
	FBal0005462
References ( 52 )

Generate a list of	All references relating to this allele ( 52 )

List References by type	Research paper ( 43 ) Supplementary material ( 1 ) Review ( 1 ) Abstract ( 6 ) Letter ( 1 )
Recent research papers ( 1 )
	Tokhunts et al., 2010, J. Biol. Chem. 285(4): 2562--2568 The full-length unprocessed hedgehog protein is an active signaling molecule. [FBrf0209740] Show all research papers ...
Recent reviews (0)
	All reviews listed in FlyBase were published before 2010 Show reviews ...

Allele Dmel\hhbar3

Allele Dmel\hh^bar3