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General Information
Symbol
Dmel\hhhs.PI
Species
D. melanogaster
Name
heat shock construct of Ingham
FlyBase ID
FBal0031488
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-hh, hs-hhM11, hsp70-hh
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

An Hsp70 promoter is fused upstream of a SmaI-EcoRV fragment carrying the entire hh open reading frame.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In

abdominal tergite & microchaeta | conditional ts

Detailed Description
Statement
Reference

14 days old hhhs.PI adult mutants (cultured at 29[o]C to activate expression) display locomotor defects and increased lethality compared to controls and also show lamellar inclusions in brain neurons.

Females in which hhhs.PI is expressed using heat shock twice daily for 3 days followed by a 3 day "chase" show excess follicle stem cell progeny in between egg chambers.

hhhs.PI animals that undergo heatshock during larval stages produce animals with severe head capsule defects.

Overexpression of hhhs.PI (using heat shock) results in an increase in larval eye neurons and optic lobe precursors. Optic lobe and Bolwig's organ tissue is induced in the head midline, resulting in a "cyclops" phenotype (the phenocritical period for this phenotype is between 2.5 and 5 hours).

Heatshock induced expression of hhhs.PI leads to excess follicle cell proliferation and the production of ectopic polar cells. Actin fibres are sometimes, though not always, seen to circle around ectopic polar cells.

Overexpression of hhhs.PI in females results in 87% of germaria having no visible fusome. No spectrosome is visible in early germ cells.

Heat shock treatment of flies carrying one copy of hhhs.PI during the first 5 hours after puparium formation (APF) has no effect on the abdominal cuticle. Heat shocks between 5 and 10 hours APF result in the transformation of posterior tergite microchaetae into macrochaetae. Flies subjected to a single 2-hour heat shock between 11 and 17 hours APF usually appear normal, although weak disturbances in bristle polarity are occasionally seen. Heat shock treatment between 18 and 35 hours APF results in an anterior expansion of the pigment band of the abdominal tergites without any effect on bristle pattern. Flies carrying two copies of hhhs.PI and subjected to three to four 2-hour heat shocks between 5 and 30 hours APF have compressed abdominal tergites which are approximately half their normal width, while the intersegmental membrane is considerably expanded. The polarity of structures in the anterior region of the tergite is sometimes completely reversed, and mirror-image posterior duplications are seen in many abdominal sternites.

Heat induced expression causes expansion of the germarium and excess follicle cells bud off newly formed egg chambers.

Ectopic hh product has little or no effect on smo mutants.

Ectopic activation of hh in the ovaries results in the overproliferation of somatic cells. Excess follicle cells fail to associate with germ-line cysts and accumulate between egg chambers, forming giant 'stalks' which are not formed by true stalk cells.

Heat shock applied for 1 hour twice a day over three days causes a dramatic increase in the number of somatic cells contributing to each ovariole, with excess somatic cells accumulating between the egg chambers. Terminal filament, cap cell and germaria region 1 look normal. Region 2 is swollen and densely packed with cells. Germaria containing hhhs.PI transplanted into non-transgenic hosts show the same effects on ovariole morphology. Chambers that have separated from the germarium are connected by giant stalks containing 25-130+ cells. Ectopic expression of hh affects the positioning of the oocyte within egg chambers and polar cell differentiation, but not the rate of germ line stem cell division.

Overexpression between 3-4 hours of development causes extensive hypertrophy of heart precursor cells.

Failed to induce programmed cell death (PCD) in Df(3L)H99 embryos.

Ectopic expression of hhhs.PI in the anterior compartment of the wing disc results in the duplication of anterior wing structures with mirror image symmetry.

Heterozygotes heat shocked for 5 minutes at 8 hours after egg laying show an increase in rows of 3o cell types at the expense of 4o cell types, with no change to the proportion of the segment made up of 2o cell types. Heterozygotes heat shocked for 20 minutes at 8 hours after egg laying show an increase in rows of 2o cell types at the expense of 3o, and some 4o, cell types. The row of 4o cells closest to the wg expressing cells are unaffected, indicating that they are not competent to respond to increased hh signal. By heat shocking homozygotes, the most extreme degree of transformation is achieved, with increasing levels of hh producing more proximal fates.

After heat shock individuals display abnormal denticle belts. Multiple heat shocks during third larval instar causes wing defects: disorganised wing veins in the anterior compartment and small expansion of the anterior-dorsal wing blade.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference

hhhs.PI is a suppressor of abnormal size | oogenesis | oogenesis phenotype of piwi2

NOT Suppressor of
Statement
Reference

hhhs.PI is a non-suppressor of female sterile phenotype of fs(1)Yb1

hhhs.PI is a non-suppressor of female sterile phenotype of piwi2

Other
Phenotype Manifest In
Suppressed by
Statement
Reference

hhhs.PI has phenotype, suppressible by mam10/mam[+]

hhhs.PI has phenotype, suppressible by Df(2R)BSC18/+

hhhs.PI has phenotype, suppressible by mam8/mam[+]

Suppressor of
Statement
Reference

hhhs.PI is a suppressor | partially of germarium phenotype of fs(1)Yb1

hhhs.PI is a suppressor | partially of phenotype of fs(1)Yb72

hhhs.PI is a suppressor of egg chamber phenotype of piwi2

hhhs.PI is a suppressor of germarium phenotype of piwi2

NOT Suppressor of
Statement
Reference

hhhs.PI is a non-suppressor of embryonic/larval dorsal vessel phenotype of wgl-12

Other
Additional Comments
Genetic Interactions
Statement
Reference

The phenotype of excess follicle stem cell progeny in between egg chambers that is caused by expression of hhhs.PI using heat shock twice daily for 3 days followed by a 3 day "chase" is dominantly suppressed by Df(2R)BSC18, mam8 or mam10, such that the number of inappropriate cells between egg chambers is strongly reduced.

Heat-shocking fs(1)Yb1 heterozygotes that carry one or two copies of the hhhs.PI transgene induces the formation of large stalks composed of overproliferated somatic cells. Over expression of hhhs.PI in piwi2 homozygotes or heterozygotes stimulates somatic cell differentiation in ovarioles. hhhs.PI also restores germ stem cell divisions in these mutants. Rescued homozygotes often contain a full complement of germline cells. Moreover the germaria generate more egg chambers, per ovariole, similar to heterozygous controls. However fertility is not restored.

0% and 5% of ph-p410/+; en54/+ double heterozygotes have a gap in the fourth wing vein at 25oC and 29oC respectively. The penetrance of the phenotype at 29oC is increased to 25% if the flies are also expressing hhhs.PI.

Heart formation cannot be restored in hhhs.PI, wgl-12 embryos that are heat shocked between 3 and 4 hours of development. hh8, hhhs.PI embryos that are heat shocked between 3 and 4 hours of development exhibit rescue (and hypertrophy) of heart precursors.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Carried in plasmid "hs-hh", expressed in Kc cells alone or with por to test the effect of por on hh processing.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
Reported As
Symbol Synonym
Name Synonyms
heat shock construct of Ingham
Secondary FlyBase IDs
    References (35)