Defects in encapsulation of the 16-cell cyst and separation from the germarium are seen in egg chambers of mirr6D1/mirre48 females. A reduction or loss of dorsal structures is seen in egg chambers; in stage 14 egg chambers the operculum has only 30 +/- 2.6 cell imprints (compared to the wild type number of 43 +/- 5.2). Dorsal appendages may be missing. Homozygous clones result in ventralisation of the egg chamber.
mirr6D1 is a suppressor of dorsal appendage phenotype of cicfet-U6/cicfet-T6
mirr6D1 is a suppressor of dorsal appendage phenotype of cicfet-U6/cicfet-E11
mirr6D1 is a suppressor of egg phenotype of cicfet-U6/cicfet-E11
mirr6D1 is a suppressor of dorsal appendage phenotype of cicfet-U6/cicBA53
Approximately 0.5% of cicfet-T6/mirr6D1 cicfet-U6 females exhibit a weak eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally with a trace of ventral collar material. Approximately 42.6% of cicfet-U6/mirr6D1 cicfet-T6 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 42.1% of cicfet-U6/mirr6D1 cicfet-T6 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. None of the cicfet-U6/mirr6D1 cicfet-T6 females exhibit a severe eggshell phenotype (compared to 1.7% in the heterozygote). There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicfet-T6/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicfet-T6/cicfet-U6 phenotype.
Approximately 34.4% of cicfet-E11/mirr6D1 cicfet-U6 females exhibit a weak eggshell phenotype. Approximately 37.4% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a weak-moderate eggshell phenotype. Approximately 24.5% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate eggshell phenotype. There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicfet-E11/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicfet-E11/cicfet-U6 phenotype.
Approximately 34.4% of cicfet-E11/mirr6D1 cicfet-U6 females exhibit a weak eggshell phenotype. Approximately 37.4% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a weak-moderate eggshell phenotype. Approximately 24.5% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate eggshell phenotype. Approximately 37.9% of cicfet-U6/mirr6D1 cicfet-E11 females exhibit a moderate-severe eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a thick ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicfet-E11/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicfet-E11/cicfet-U6 phenotype.
Approximately 0.5% of cicfet-U6/mirr6D1 cicBA53 females exhibit a moderate eggshell phenotype. The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a ventral collar of ectopic appendage-like material. The dorsalmost region remains free of appendage material. Approximately 32.6% of cicfet-U6/mirr6D1 cicBA53 females exhibit a severe eggshell phenotype (compared to 85.9 in the heterozygote). The dorsal midline is expanded and the appendages are broader and positioned laterally and exhibit a pronounced ventral collar of ectopic appendage-like material, along with small anterior projections.There is an increase in the weak phenotype, and a decrease in the severe phenotype compared to the cicBA53/cicfet-U6 mutant, indicating that mirr6D1 suppresses the cicBA53/cicfet-U6 phenotype.
