FlyBase Allele Report: Dmel\Ilk[1]

General Information

Symbol

Dmel\Ilk¹

Species

D. melanogaster

Name

FlyBase ID

FBal0031864

Feature type

allele

Associated gene

Dmel\Ilk

Associated Insertion(s)

Carried in Construct

Also Known As

l(3)78Ca¹

Key Links

Genomic Maps

JBrowse

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl nitrosourea

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Zervas et al., 2001)

Mutagen

ethyl nitrosourea

(Russell et al., 1996, Carpenter, 1994)

Progenitor genotype

Cytology

Description

Amino acid replacement: W211term.

(Zervas et al., 2001)

Nucleotide substitution: G?A.

(Zervas et al., 2001)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

point_mutation

3L:21,218,167..21,218,167 [+]

Nucleotide change:

G21218167A

Reported nucleotide change:

G?A

Amino acid change:

W211term | Ilk-PA

Reported amino acid change:

W211term

Comment:

G to A nucleotide change at the second or third position of the Trp codon leads to a nonsense mutation. (exact site of mutation unspecified)

(Zervas et al., 2001)

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

adult posterior midgut epithelium | somatic clone

(Lin et al., 2013)

larval somatic muscle cell

(Zervas et al., 2001)

larval somatic muscle cell (with Df(3L)Pc-14d)

(Zervas et al., 2001)

wing | somatic clone

(Zervas et al., 2001)

Detailed Description

Statement

Reference

Ilk¹/+ does not significantly affect resistance to ethanol-induced sedation.

(Ojelade et al., 2015)

Adult-generated Ilk¹ homozygous mutant intestinal stem cell clones have reduced maintenance 7 days and 14 days after clone induction compared to control clones; cells in the remaining clones are either diploid cells or immature enterocytes.

(Lin et al., 2013)

Ilk¹ dorsal branch terminal cell clones do not cause any detectable defects in terminal cell morphology.

(Levi et al., 2006)

Homozygotes die at the end of embryogenesis. The cuticle is completely normal in Ilk¹/Df(3L)Pc-14d embryos. Midgut development is normal in mutant embryos. Stage 17 homozygous and Ilk¹/Df(3L)Pc-14d embryos show defects in most of the muscles, with actin clumped together rather than extending along the length of the muscles as in wild type. Muscles where the actin has detached from the plasma membrane and the membrane remains attached at its normal position adjacent to the extracellular matrix are seen in Ilk¹/Df(3L)Pc-14d embryos. Muscles where both the plasma membrane and actin have retracted are also seen, although they are still separate. Homozygous embryos derived from homozygous female germline clones (lacking both maternal and zygotic Ilk function) have a modestly more severe phenotype than homozygous embryos (lacking zygotic Ilk function); they show clumping of actin in the muscles slightly earlier, at stage 16. These embryos have normal cuticles. Homozygous clones in the wing result in a wing blister. Wing blisters are associated with clones on either side of the wing blade.

(Zervas et al., 2001)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressor of

Statement

Reference

Ilk[+]/Ilk¹ is a suppressor of chemical resistant | adult stage phenotype of Scer\GAL4^elav.PU, ics^RNAi.UAS.WIZ

(Ojelade et al., 2015)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

Ilk¹/+ significantly suppresses (to wild type levels) the increased resistance to ethanol-induced sedation seen in flies with expression of ics^{dsRNA.Scer\UAS.WIZ} driven by Scer\GAL4^elav.PU.

(Ojelade et al., 2015)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Fails to complement

Ilk²

(Zervas et al., 2001)

Rescued by

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^mGFP6

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^T384A.mGFP6

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^T384D.mGFP6

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Scer\GAL4^how-24B/Ilk^UAS.mGFP6

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Scer\GAL4^how-24B/Ilk^{T384A.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Scer\GAL4^how-24B/Ilk^{T384D.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Scer\GAL4^how-24B/Ilk^{S176G.T180G.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^{S176D.T180D.UAS.mGFP6}/Scer\GAL4^how-24B

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is rescued by Scer\GAL4^how-24B/Ilk^{F436A.UAS.mGFP6}

(Zervas et al., 2011)

Ilk¹ is rescued by Ilk^K219M.mGFP6

(Zervas et al., 2001)

Ilk¹ is rescued by Ilk^E359K.mGFP6

(Zervas et al., 2001)

Ilk¹ is rescued by Ilk^E359K.UAS/Scer\GAL4^how-24B

(Zervas et al., 2001)

Ilk¹ is rescued by Ilk^P358S.mGFP6

(Zervas et al., 2001)

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^+t9

(Zervas et al., 2001)

Df(3L)Pc-14d/Ilk¹ is rescued by Ilk^mGFP6

(Zervas et al., 2001)

Partially rescued by

Df(3L)Pc-14d/Ilk¹ is partially rescued by Ilk^{Δ34-177.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Ilk^{R208D.R210D.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Ilk^{R208D.R210D.Δ34-177.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Scer\GAL4^how-24B/Ilk^{Δ34-177.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Ilk^{R208D.R210D.UAS.mGFP6}/Scer\GAL4^how-24B

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Scer\GAL4^how-24B/Ilk^{R208D.R210D.Δ34-177.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is partially rescued by Scer\GAL4^how-24B/Ilk^UAS.cZa

(Zervas et al., 2001)

Not rescued by

Df(3L)Pc-14d/Ilk¹ is not rescued by Ilk^{Δ178-448.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is not rescued by Ilk^{Δ34-177.mGFP6}/Ilk^{Δ178-448.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is not rescued by Scer\GAL4^how-24B/Ilk^{Δ178-448.UAS.mGFP6}

(Zervas et al., 2011)

Df(3L)Pc-14d/Ilk¹ is not rescued by Scer\GAL4^how-24B/Ilk^{Δ34-177.UAS.mGFP6}/Ilk^{Δ178-448.UAS.mGFP6}

(Zervas et al., 2011)

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4861

mwh¹ Ilk¹ red¹ e⁴/TM3, Sb¹

Kyoto

107914

mwh¹ Ilk¹ red¹ e⁴/TM3, Sb¹

Notes on Origin

Discoverer

A.T.C. Carpenter.

(Carpenter, 1994)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

ILK¹

(Thuveson et al., 2019)

Ilk¹

(Sarov et al., 2016, Ojelade et al., 2015, Christensen et al., 2009.2.28)

ilk¹

(Keramidioti et al., 2022, Lin et al., 2013, Elias et al., 2012, Knox et al., 2011, Zervas et al., 2011, Loer et al., 2008, Levi et al., 2006, Zervas et al., 2001)

l(3)78Ca¹

(Zervas et al., 2001, Russell et al., 1996, Carpenter, 1994)

Name Synonyms

Secondary FlyBase IDs

References (15)

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