Embryo hatching rate is somewhat reduced in Rab1193Bi/Rab1193Bi mutants compared to wild type. Embryo hatching rate is massively reduced in Rab1193Bi/Rab11j2D1 mutants (around 10% hatch) compared to wild type.
Rab1193Bi mutants exhibit synaptic overgrowth with excess satellite boutons on neuromuscular junction 4.
Homozygotes show a small increase in the total number of multi-nucleate spermatids compared to controls.
Testes from Rab1193Bi homozygous larvae, or larvae in which Rab1193Bi is transheterozygous with any of Rab11EP3017, Rab11ETo11 or Rab11ETo3, have spermatids composed of an abnormally large Nebenkern associated with multiple nuclei.
Mid and late telophases in Rab1193Bi/Rab11ETo11 spermatocytes display contractile rings that are not fully constricted or poorly constricted and broken into irregular pieces. Defective central spindles are seen in 80% of these cases. In those spermatocytes that failed to complete cytokinesis, the furrow ingresses to a limited extent and then regresses, coincident with disassembly of the central spindle.
48% of telophases in Rab1193Bi/Rab11ETo11 spermatocytes display abnormal localization of Golgi-derived vesicle-like structures.
Rab1193Bi/Rab11ETo11 testes show disorganized acroblasts in both early and (particularly) elongating spermatids.
Rab1193Bi/Rab11ETo11 brains do not exhibit polyploid mitoses.
Rab1193Bi/+ flies show no eye phenotype.
Rab11ex1/Rab1193Bi transheterozygotes are mostly lethal but a few survive to adulthood.
The posterior abdomens of Rab11ex1/Rab1193Bi flies have empty sockets that are missing microchaetae. Additionally, posterior scutellar bristles are shortened in these mutants. Rab1193Bi homozygotes also show microchaetae loss, although this is less extensive.
The synapse of muscle 6/7 shows an increase in boutons by about 60% in Rab11ex1/Rab1193Bi mutants compared to controls. The boutons of Rab11ex1/Rab1193Bi mutants are often clustered resulting in a 'bunch of grapes' morphology instead of the wild-type 'beads-on-a-string' morphology. The aberrant morphology may reflect increased branching (by 2.6 fold) of Rab11ex1/Rab1193Bi boutons.
8% of Rab1193Bi/Rab11j2D1-derived embryos exhibit disrupted nuclear morphology in premigration and early cortical divisions. During the late cortical divisions, when the nuclei are more densely packed, 65% of embryos exhibit severely disrupted nuclear morphology. In Rab1193Bi/Rab11j2D1-derived nuclear cycle 12 embryos, actin cap formation occurs normally. As the embryos progress into prophase, the actin caps are dismantled as the actin re-organises into furrows encompassing each prophase nucleus and its developing spindle. In Rab1193Bi/Rab11j2D1-derived embryos, the hexagonal furrow network is riddled with gaps, present at prophase during the initial stages of furrow formation. Similar defects are observed during cellularisation at nuclear cycle 14.
Homozygotes have weak bristle defects. 7.7% of eggs laid by Rab1193Bi/Rab11j2D1 females develop into larvae. 2% of the escapers have a germ cell-less phenotype.