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General Information
Symbol
Dmel\Rab1193Bi
Species
D. melanogaster
Name
FlyBase ID
FBal0031959
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C21114900T

Amino acid change:

R104W | Rab11-PA; R104W | Rab11-PB

Reported amino acid change:

R104W

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: R104W.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Embryo hatching rate is somewhat reduced in Rab1193Bi/Rab1193Bi mutants compared to wild type. Embryo hatching rate is massively reduced in Rab1193Bi/Rab11j2D1 mutants (around 10% hatch) compared to wild type.

Rab1193Bi mutants exhibit synaptic overgrowth with excess satellite boutons on neuromuscular junction 4.

Homozygotes show a small increase in the total number of multi-nucleate spermatids compared to controls.

Testes from Rab1193Bi homozygous larvae, or larvae in which Rab1193Bi is transheterozygous with any of Rab11EP3017, Rab11ETo11 or Rab11ETo3, have spermatids composed of an abnormally large Nebenkern associated with multiple nuclei.

Mid and late telophases in Rab1193Bi/Rab11ETo11 spermatocytes display contractile rings that are not fully constricted or poorly constricted and broken into irregular pieces. Defective central spindles are seen in 80% of these cases. In those spermatocytes that failed to complete cytokinesis, the furrow ingresses to a limited extent and then regresses, coincident with disassembly of the central spindle.

48% of telophases in Rab1193Bi/Rab11ETo11 spermatocytes display abnormal localization of Golgi-derived vesicle-like structures.

Rab1193Bi/Rab11ETo11 testes show disorganized acroblasts in both early and (particularly) elongating spermatids.

Rab1193Bi/Rab11ETo11 brains do not exhibit polyploid mitoses.

Rab1193Bi/+ flies show no eye phenotype.

Rab11ex1/Rab1193Bi transheterozygotes are mostly lethal but a few survive to adulthood.

The posterior abdomens of Rab11ex1/Rab1193Bi flies have empty sockets that are missing microchaetae. Additionally, posterior scutellar bristles are shortened in these mutants. Rab1193Bi homozygotes also show microchaetae loss, although this is less extensive.

The synapse of muscle 6/7 shows an increase in boutons by about 60% in Rab11ex1/Rab1193Bi mutants compared to controls. The boutons of Rab11ex1/Rab1193Bi mutants are often clustered resulting in a 'bunch of grapes' morphology instead of the wild-type 'beads-on-a-string' morphology. The aberrant morphology may reflect increased branching (by 2.6 fold) of Rab11ex1/Rab1193Bi boutons.

8% of Rab1193Bi/Rab11j2D1-derived embryos exhibit disrupted nuclear morphology in premigration and early cortical divisions. During the late cortical divisions, when the nuclei are more densely packed, 65% of embryos exhibit severely disrupted nuclear morphology. In Rab1193Bi/Rab11j2D1-derived nuclear cycle 12 embryos, actin cap formation occurs normally. As the embryos progress into prophase, the actin caps are dismantled as the actin re-organises into furrows encompassing each prophase nucleus and its developing spindle. In Rab1193Bi/Rab11j2D1-derived embryos, the hexagonal furrow network is riddled with gaps, present at prophase during the initial stages of furrow formation. Similar defects are observed during cellularisation at nuclear cycle 14.

Homozygotes have weak bristle defects. 7.7% of eggs laid by Rab1193Bi/Rab11j2D1 females develop into larvae. 2% of the escapers have a germ cell-less phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference
NOT Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

Rab1193Bi has spermatid phenotype, enhanceable by bru[+]/brunZ3358

Suppressed by
Enhancer of
Statement
Reference
NOT Enhancer of
Statement
Reference

Rab1193Bi/Rab11ETo11 is a non-enhancer of spermatid phenotype of vibEP513

NOT Suppressor of
Statement
Reference

Rab1193Bi/Rab11ETo11 is a non-suppressor of spermatid phenotype of fwdZ0453/fwd3

Rab1193Bi/Rab11ETo11 is a non-suppressor of spermatid phenotype of vibEP513

Other
Additional Comments
Genetic Interactions
Statement
Reference

Bsg25D1 leads to a small but significant enhancements of reduced embryo hatching rates in Rab1193Bi/Rab1193Bi or Rab1193Bi/Rab11j2D1 mutants.

Rab1193Bi, Madk00237 double mutants exhibit undergrown synapses with few boutons on neuromuscular junction 4 that are indistinguishable from Madk00237 single mutants.

Acsl05847/AcslKO Rab1193Bi/+ animals do not survive to wandering third instar larvae.

Rab1193Bi/+ and, to a greater extent, Rab1193Bi/Rab1193Bi significantly increases neuromuscular junction overgrowth of Acsl05847/Acsl8 mutants.

Flies homozygous for both bruZ3358 and Rab1193Bi die in early larval stages at 25[o]C, as do flies homozygous for bruZ3358 and transheterozygous for Rab1193Bi/Rab11ETo3.

bruZ3358/+ males also homozygous for Rab1193Bi display a four-fold increase in the total number of multi-nucleate spermatids compared with males homozygous for Rab1193Bi alone.

Rab1193Bi/Rab11ETo11, fwd3/fwdZ0453 spermatids are multinucleated at a similar frequency to fwd3/fwdZ0453 spermatids.

Rab1193Bi/Rab11ETo11, vibEP513 spermatids are multinucleated at a similar frequency to vibEP513 spermatids.

Rab1193Bi heterozygotes act as dominant enhancers of the Scer\GAL4GMR.PF>bchsEP2299 eye phenotype, resulting in adults with severely reduced eyes.

The viability of Rab11ex1/Rab1193Bi is increased when flies are also mutant for either Df(2L)cl7/+, Df(2L)cl7/bchs17, bchs17/bchs12 or bchs17/+.

The microchaeta loss of Rab11ex1/Rab1193Bi flies is significantly suppressed by Df(2L)cl7/+ or bchs17/+ and is completely suppressed by Df(2L)cl7/bchs17 or bchs17/bchs12. Further, posterior scutellar bristle length of Rab11ex1/Rab1193Bi flies is restored partially by Df(2L)cl7/+ or bchs17/+ and more fully by Df(2L)cl7/bchs17 or bchs17/bchs12.

The microchaeta loss of Rab1193Bi flies is significantly suppressed by bchs58/+ or bchs8K/+ and is completely suppressed by bchs58/bchs58.

The bouton density and branching defects of Rab11ex1/Rab1193Bi larval NMJs at muscle 6/7 are significantly suppressed in bchs17/bchs12; Rab11ex1/Rab1193Bi double mutants.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Rab1193Bi/Rab11ETo3 or Rab1193Bi/Rab11ETo11 flies carrying Rab11T:Avic\GFP are fully viable and testes from larvae of these genotypes do not exhibit abnormal spermatids.

Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

T. Vincent

Comments
Comments

Order of alleles (based on transheterozygous sterile phenotype): Rab1193Bi < Rab11j2D1 < Rab11EP3017 = Rab11ETo11 = Rab11ETo3 < Df(3R)e-N19.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
References (15)