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General Information
Symbol
Dmel\dco3
Species
D. melanogaster
Name
FlyBase ID
FBal0032016
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
G31057632A
Amino acid change:
E74K | dco-PA; E74K | dco-PB; E74K | dco-PC; E74K | dco-PD; E74K | dco-PE
Reported amino acid change:
E74K
Comment:
One of two amino acid changes observed in dco3. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
point mutation
Nucleotide change:
C31057842T
Amino acid change:
R4C | dco-PA; R4C | dco-PB; R4C | dco-PC; R4C | dco-PD; R4C | dco-PE
Reported amino acid change:
R4C
Comment:
One of two amino acid changes observed in dco3. Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
The mutated residues R4 and E74 fall in the catalytic domain of the wild type protein. E74 lies in the region of the catalytic domain thought to face the protein substrates, not in the region involved in the catalytic activity. dco2 and dco3 share a D401Q polymorphism.
Amino acid replacement: R4C.
Amino acid replacement: E74K.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
dco3 mutant clones in the eye imaginal disc are overgrown compared to controls.
The overall morphology of dco3 mutants is abnormal, with extensive rippling and loss of normal morphological features including the wing pouch. dco3/dcot.L39Q transheterozygotes are similar to dco3 mutants.
The rough eye phenotype observed in Fmr1sev.PW flies is suppressed in a dco3/+ background.
dco3 clones in the adult abdomen do not show planar cell polarity defects.
dco3 clones in the wing imaginal disc are larger than their associated wild type sibling clones.
dco3 mutant wing discs collected 36-48 hours after the L2-L3 molt are overgrown.
dco3/dcole88 wing discs exhibit an overgrowth phenotype.
Homozygotes show lethality in larval or early pupal stages, with overgrown discs. Pharate adults generated with dco3/hypomorph combinations show morphogenetic aberrations including duplicated structures. dco3 in combination with dco2 or dco18 : lethality in larval or early pupal stages, with overgrown discs. dco3 in combination with dcole88, dcoi3-193, dcoS053813, dcoS139602, dcoS091510 and dcoP103 : show lethality during larval or early pupal stages with typical dco3 phenotype (swollen tarsi with or without head defects). Discs overgrown. dco3 in combination with dcoS144701 : adult viable with reduced eyes extra vibrissae and expanded wings. dco3 somatic clones in the discs show an autonomous overgrowth phenotype. Cell size is unaffected. Animals with many clones show delayed pupariation.
Hyperplastic overgrowth mutant. Apico-lateral junctional complexes appear normal.
Hyperplastic imaginal disc overgrowth, affecting all discs. Discs implanted into wild type hosts for metamorphosis over grow and differentiate abnormal imaginal structures. dco18/dco3 mutant larvae grow at a normal rate until end of feeding period of last larval instar, when growth slows and then stops: they eventually reach double the weight of wild type pupariating larvae. The ecdysteroid secretory activity of dlg1 and dco ring glands in vitro is 1/6 that of wild type.
Larval life prolonged by up to 14 days. Overgrown larvae show bloating and an increase in water content.
When raised at 25oC the larval period of homozygotes and interallelic heterozygotes is greatly extended and they develop overgrown imaginal discs. Imaginal discs show gap-junctional communication defects: coupling is severely diminished. The single layer epithelium is maintained during overgrowth and they differentiate into the correct but abnormal adult structures.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Enhancer of
Statement
Reference
dco[+]/dco3 is an enhancer of cell polarity defective phenotype of fz20/fz19
Phenotype Manifest In
Suppressed by
Statement
Reference
dco3 has wing disc | larval stage phenotype, suppressible by d1
Enhancer of
Statement
Reference
dco[+]/dco3 is an enhancer of ommatidium phenotype of fz20/fz19
Suppressor of
Statement
Reference
dco[+]/dco3 is a suppressor of eye phenotype of Fmr1sev.PW
Other
Additional Comments
Genetic Interactions
Statement
Reference
The increased size of dco3 clones is rescued by Fbxl7Scer\UAS.T:Zzzz\FLAG expression.
The lethality and overgrowth phenotypes of dco3 animals are rescued by Scer\GAL4tub.PU-mediated overexpression of wtsScer\UAS.T:Hsap\MYC, resulting in animals that, aside from some mild wing vein phenotypes, are indistinguishable from wild type animals over-expressing wtsScer\UAS.T:Hsap\MYC.
d1 suppresses the wing disc overgrowth seen in dco3 mutant larvae.
The ommatidial polarity defects of fz19/fz20 mutants are enhanced in fz19/fz20, dco3/+ double mutants.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (3)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (22)