E74K | dco-PA; E74K | dco-PB; E74K | dco-PC; E74K | dco-PD; E74K | dco-PE
R4C | dco-PA; R4C | dco-PB; R4C | dco-PC; R4C | dco-PD; R4C | dco-PE
The mutated residues R4 and E74 fall in the catalytic domain of the wild type protein. E74 lies in the region of the catalytic domain thought to face the protein substrates, not in the region involved in the catalytic activity. dco2 and dco3 share a D401Q polymorphism.
Amino acid replacement: R4C.
Amino acid replacement: E74K.
The lethality and overgrowth phenotypes of dco3 animals are rescued by Scer\GAL4tub.PU-mediated overexpression of wtsScer\UAS.T:Hsap\MYC, resulting in animals that, aside from some mild wing vein phenotypes, are indistinguishable from wild type animals over-expressing wtsScer\UAS.T:Hsap\MYC.
Assessment of complementation based on lethal aspect of phenotype.