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General Information
Symbol
Dmel\dcole88
Species
D. melanogaster
Name
FlyBase ID
FBal0032019
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Deletion of 369bp that removes the last intron/exon boundary and ends after the T of the ATG initiation codon.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Deletion of 369bp in the dco transcription unit, at the 5' end of the ORF, removing the last intron/exon boundary and ends after the T of the ATG initiation codon.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The presence of dcot.L39Q in a dcole88/Df(3R)A177der20 background results in an extended larval development (10 days compared to 5 days for wild-type larvae) associated with a striking overgrowth phenotype in larval imaginal discs. Whereas the wild-type disc stop growing when they reach the appropriate size, the mutant discs continue growing during the extended larval period and before pupariation they reach about three times the normal final size. Although the mutant larvae eventually pupate they do not proceed through metamorphosis, and the pupae die without any signs of adult development. The overall morphology of these mutants is abnormal, with extensive rippling and loss of normal morphological features including the wing pouch. These mutant discs retain their monolayered epithelial structure showing that they exhibit a hyperplastic overgrowth phenotype.

The presence of dcot.L39Q and dcot.S101R in a dcole88/Df(3R)A177der20 background results in an extended larval development period. The discs do not grow larger than normal, but the tissue seems to disintegrate in places, indicating a loss of tissue integrity.

The presence of dcot.L39Q and dcot.S101R in a dcole88/Df(3R)A177der20 background results in a very mild dominant effect on a crossvein in the adult wing, with less than 15% penetrance.

dco3/dcole88 wing discs exhibit an overgrowth phenotype.

Homozygotes show larval lethality with a discless phenotype. dco3 in combination with dcole88, dcoi3-193, dcoS053813, dcoS139602, dcoS091510 and dcoP103 : show lethality during larval or early pupal stages with typical dco3 phenotype (swollen tarsi with or without head defects). Discs overgrown. dco2 in combination with dcole88 : show lethality during larval or early pupal stages. dco18 in combination with dcole88, dcoi3-193, or dcoS053813 : show lethality during larval stages. dcole88 in combination with dcoi3-193 or dcoS053813 : show lethality during larval stages with a discless phenotype. dcole88 in combination with dcoS139602 : show lethality during larval or early pupal stages with a discless phenotype. dcole88 in combination with dcoS091510 : show lethality during larval or early pupal stages with a small discs phenotype. dcole88 in combination with dcoP103 : show lethality during late pupal stage with abnormal discs with granular zone and abnormal folding. dcole88 in combination with dcoS144701 : adult viable with reduced eyes, disordered facets and minor wing vein defects. In clones induced in the imaginal discs, homozygous dcole88 cells are growth inhibited and fail to survive. This growth inhibition and apoptosis is cell autonomous.

No vitellogenic egg primordia develop in chimeric females containing homozygous dcole88 germ cells.

Homozygotes are smaller than wild type at 4-5 days after egg laying. Larvae have correctly positioned imaginal discs. When homozygous or in heteroallelic combinations the imaginal discs degenerate producing a discless phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Enhanced by
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference

dcole88 mutant clones generated in an Scer\GAL4en-e16E/BacA\p35Scer\UAS.cHa background are consistently smaller than twin spots outside this background. The dcole88 clones are on average 4 times smaller than the twin spots in the posterior compartment. Because the twins and clones originate from the two daughter cells of a single cell that underwent mitotic recombination, they grow for the same period of time in a similar location. Therefore the approimxate 1:4 ratio of clone/twin size in dcole88 mutants suggests a defectin the rate of growth/proliferation.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Allelic series in order of decreasing strength: dcole88 > dco18 > dco2.

Pole cell transplantation shows that the dcole88 mutation interferes with germ-line development.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
References (10)