Allele Dmel\lab^hs.PH

General Information
Symbol	Dmel\lab^hs.PH	Species	D. melanogaster
Name	heat shock construct of Heuer	FlyBase ID	FBal0032046
Feature type	allele	Associated gene	Dmel\lab

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Heuer and Kaufman, 1992)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Construct: lab gene expression is driven heat shock promoter. (Heuer and Kaufman, 1992)
Carried in construct	P{hslab} (Kuzin et al., 1997, Page, 2000, Gould et al., 1997, Heuer and Kaufman, 1992, Popperl et al., 1995, Macias and Morata, 1996, Hirth et al., 1998, Keegan et al., 1997)
Cytology
Phenotypic Data
Phenotypic Class
	wild-type (Page, 2000)
Phenotype Manifest In
	arista (Kuzin et al., 1997) cuprophilic cell (Hoppler and Bienz, 1994) midgut interstitial cell (Hoppler and Bienz, 1994)
Detailed Description
	Statement Reference Heat induced expression does not affect tarsal leg structures but causes some thickening of the arista. (Kuzin et al., 1997) Heat shocked lab^hs.PH larvae lack sclerotic plates but differentiate in every thoracic and abdominal segment a stereotyped arch-shaped cephalic structure which appears in the ventral region and results from the fusion of material at the left and right sides. (Macias and Morata, 1996) Heat shock treatments between 7 and 9 hours of embryonic development generate extra copper cells posterior to the normal copper cell domain in the larval midgut. Large flat cells and interstitial cells are transformed into cuprophilic cells. This effect is dependent on endogenous lab function. Heat shock after 11 hours of development generates cuprophilic cells with 'banana shapes' abnormally near the anterior margin of the copper cell domain. A single heat pulse between 9 and 11 hours of development frequently results in severe or virtually complete repression of copper cell development, indicating that lab autoregulation in the midgut can be negative. (Hoppler and Bienz, 1994) Double antibody staining with F2 and P12α-85E revealed the T1-T3 es organs to be abnormal, lch5 abnormal in some A segments and dch3 partially transformed to lch3 in T2 and T3. (Heuer and Kaufman, 1992)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Other
Statement Reference lab^hs.PH, ss^a40aw has visible phenotype (Kuzin et al., 1997, Kuzin et al., 1997) lab^hs.PH, ss^aSc has visible phenotype (Kuzin et al., 1997, Kuzin et al., 1997)
Phenotype Manifest In
Suppressor of
Statement Reference lab^hs.PH is a suppressor of embryonic epidermis phenotype of Abd-B^M8, Antp^Ns-rvC3, Scr^C1, Ubx^MX2, abd-A^M1 (Macias and Morata, 1996)
Other
Statement Reference lab^hs.PH, ss^a40aw has third segment of antenna phenotype (Kuzin et al., 1997, Kuzin et al., 1997) lab^hs.PH, ss^aSc has antennal segment 3 & macrochaeta phenotype (Kuzin et al., 1997, Kuzin et al., 1997) lab^hs.PH, ss^aSc has antennal segment phenotype (Kuzin et al., 1997, Kuzin et al., 1997) lab^hs.PH, ss^aSc has tarsal segment phenotype (Kuzin et al., 1997, Kuzin et al., 1997)
Additional Comments
Genetic Interactions
Statement Reference Induction of expression in ss^aSc flies causes shortening of tarsi in all antennal and leg structures. Third segment of the antenna exhibits a multiple bristle phenotype. Induction of expression in ss^a40aw flies causes overgrowth of the third antennal segment without the formation of multiple bristles. (Kuzin et al., 1997) Scr^C1 Antp^Ns-rvC3 Ubx^MX2 abd-A^M1 Abd-B^M8 larvae (deficient for activity of thoracic and abdominal homeotic genes) exhibit sclerotic plates anterior to each denticle belt. Expression of lab^hs.PH suppresses the differentiation of the sclerotic plates. The appearance of the sclerotic plates seen in heat shocked lab^hs.PH larvae can suppressed by any homeotic gene. (Macias and Morata, 1996)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Rescues	lab^hs.PH rescues lab⁹ (Page, 2000)
Comments	Expression of lab^hs.PH between 5 and 6 hours of development (using heat shock) rescues the embryonic brain defects of lab⁹ animals; the tritocerebral commissure is restored (although it is thinner than in wild-type animals) and the longitudinal connectives are also present. Some variability in rescue is seen; approximately 85% of embryos show essentially complete restoration of the tritocerebral commissure and longitudinal connectives, 10% of embryos lack a normal tritocerebral commissure but have unfasciculated axons where this structure should be, and 5% of embryos show no rescue. Expression of lab^hs.PH at 4-5 hours and 6-7 hours of development (using heat shock) rescues the lab⁹ phenotype in approximately 10% of embryos. No rescue is seen in embryos heat shocked at 7-8, 8-9, 9-10 or 10-11 hours of development. (Page, 2000)
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 2 )
Reported As
Symbol Synonym	lab^hs.PH
Name Synonym	heat shock construct of Heuer
Secondary FlyBase IDs

References ( 9 )
Research paper	Page, 2000, Dev. Genes Evol. 210(11): 559--563 labial acts to initiate neuronal fate specification, but not axon pathfinding, in the embryonic brain of Drosophila. [FBrf0134552] Hirth et al., 1998, Development 125(9): 1579--1589 Homeotic gene action in embryonic brain development of Drosophila. [FBrf0102627] Gould et al., 1997, Genes Dev. 11(7): 900--913 Positive cross-regulation and enhancer sharing: two mechanisms for specifying overlapping Hox expression patterns. [FBrf0098982] Keegan et al., 1997, Mech. Dev. 63(2): 145--157 A sequence conserved in vertebrate Hox gene introns functions as an enhancer regulated by posterior homeotic genes in Drosophila imaginal discs. [FBrf0095419] Kuzin et al., 1997, Int. J. Dev. Biol. 41(6): 867--875 Interaction between spineless-aristapedia gene and genes from Antennapedia and bithorax complexes of Drosophila melanogaster. [FBrf0100109] Macias and Morata, 1996, EMBO J. 15(2): 334--343 Functional hierarchy and phenotypic suppression among Drosophila homeotic genes: The labial and empty spiracles genes. [FBrf0086531] Popperl et al., 1995, Cell 81(7): 1031--1042 Segmental expression of Hoxb-1 is controlled by a highly conserved autoregulatory loop dependent upon exd pbx. [FBrf0083614] Hoppler and Bienz, 1994, Cell 76(4): 689--702 Specification of a single cell type by a Drosophila homeotic gene. [FBrf0068532] Heuer and Kaufman, 1992, Development 115(1): 35--47 Homeotic genes have specific functional roles in the establishment of the Drosophila embryonic peripheral nervous system. [FBrf0055864]

Allele Dmel\labhs.PH

Allele Dmel\lab^hs.PH