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General Information
Symbol
Dmel\pckEA97
Species
D. melanogaster
Name
FlyBase ID
FBal0032381
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
megaEA97
Key Links
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

A1471409T

Amino acid change:

E97V | pck-PA; E97V | pck-PC; S117C | pck-PD

Reported amino acid change:

E97V

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: E97V.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Unlike wild-type, post stage 15 trachea in pckEA97 homozygotes are unable to exclude from their lumens, a fluorescently labelled 10kDa dextran injected into the body cavity. This is consistent with these trachea lacking a functioning septate junction barrier. The primary tracheal branches of these embryos are extremely elongated, but have almost no diameter defects.

Early tracheal development, including primary branch budding and outgrowth and lumen formation and morphology occur normally during stages 11-14. Stage 16 embryos show a number of tracheal defects. These include an increased length of the dorsal trunk compared to wild type (with no accompanying increase in cell number), mild or no defects in the diameter of the dorsal trunk and moderate defects in the diameter of other primary branches. Irregular and variable tracheal tube morphologies, with local constrictions and/or dilations are seen in all major branches. The defects are first seen at stage 15.

Cuticle is poorly pigmented, denticle belts are incompletely developed although segmentation is normal. Tracheal trunks display sclerotized internal lesions. Hemizygotes display the classical bcd phenotype of a double abdomen.

embryonic lethal no maternal effect in homozygous germ-line clones

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer

Lefevre.

Comments
Comments

Maternal germline clonal analysis demonstrates there is no maternal effect.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
  • FBal0006625
References (7)