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General Information
Symbol
Dmel\Raf400B8
Species
D. melanogaster
Name
FlyBase ID
FBal0032418
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
D-raf400B8
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

G2342467M

Amino acid change:

G441R | Raf-PA; G441R | Raf-PE

Reported amino acid change:

G484R

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: G484R. G484R falls in the CR3 domain.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

phl400B8 mutants are lethal in larvae, but such flies carrying phlhs.PD can survive to fertile adults by using daily heatshocks. If heatshocks are withdrawn on eclosion, testes from 5 day old males are indistinguishable from controls. By day 7 however, almost all mutant testes show great proliferation centre expansion. This is due to excess, early stage germ cells and continues such that on day 15 testes are filled with these cells at the expense of post-mitotic cells. Unbranched fusomes are found in may cells even those located some distance from the hub, where in wild-type testes, only branched fusomes interconnecting secondary spermatogonia are found. Although branches fusomes are found, these fusomes usually appear only after an intervening region containing many excess cells that have only spheroid fusomes. These excess germ-cells do not result from an increased frequency of germline stem cell divisions, as the M-phase index for the tier of cells adjacent to the hub in mutant testes is almost identical to that in wild-type. Homozygous mutant germ-line clones appear to be completely wild-type.

Homozygous embryos exhibit defects in posterior pattern. Injection of wild type RNA fails to rescue the defects.

Class 1 allele: Unrescued embryos (lacking maternal and zygotic phl activity) fail to differentiate into structured embryos and degenerate around 7 hrs of development. Rescued embryos (carrying wild type phl from their father) miss most structures posterior to abdominal segment 6 or 7 and anteriorly a portion of the cephalopharyngeal skeleton, labral sense organ and medial tooth are deleted. fkh expression (in wild type evident in hindgut, Malpighian tubule(s) and anal pads) entirely absent from class 1 embryos. Expression of tll, hkb, hb, fkh absent from both rescued and unrescued embryos, 7th ftz stripe deleted and 6th ftz stripe variably deleted/expanded. Conclude from 0% to 20--25% EL of blastoderm embryos deleted in class 1 alleles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Not rescued by
Comments
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

Comparison of homozygotes for this allele with heterozygotes for this allele and Df(1)64c18 (deleted for phl) is the basis for amorph designation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (5)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (7)