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General Information
Symbol
Dmel\Rafraf-1
Species
D. melanogaster
Name
FlyBase ID
FBal0032422
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
D-raf1, l(1)raf1
Key Links
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Nucleotide change:

C2342119T

Amino acid change:

Q369term | Raf-PA; Q369term | Raf-PE

Reported amino acid change:

Q412term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Amino acid replacement: Q412term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Disease-implicated variant(s)
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous embryos exhibit defects in posterior pattern.

Humeral disc cells of phlraf-1 embryos are reduced in number and arrested at the G2/M boundary.

No aberrant mitotic figures are observed at 28oC.

Humeral discs of phlraf-1 flies show reduced number of imaginal cells, while size of nuclei remains normal. Humeral discs of esg05729/esg35Ce-3 flies have nuclei similar in size to wild type, and differentiate normal dorsal T1 structures. Combination of phlraf-1 with esg05729/esg35Ce-3 greatly increased size of the imaginal cell nuclei.

Twin spot analysis demonstrates a 38% reduction of the proliferation rate. The proliferation rate is significantly recovered by Dsor1Su1.

Lethal phase is either at larval or pupal stages. The proliferating cells of both somatic and germ line origins are severely affected. The construct P{C20-Draf} confers partial rescue of the lethal phenotype.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Phenotype Manifest In
Suppressor of
Statement
Reference

Rafraf-1/phl[+] is a suppressor of phenotype of aos257

Rafraf-1 is a suppressor of phenotype of Src42AKR.hs.2sev

Additional Comments
Genetic Interactions
Statement
Reference

Twin spot analysis demonstrates a 38% reduction of the proliferation rate. The proliferation rate is significantly recovered by Dsor1Su1.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

Injection of wild type RNA rescues the filzkorper, tuft and anal pads.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (7)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (8)