mutant adult survivors exhibit a marked rough eye phenotype.
flies have rough eyes. Facet size varies from smaller than normal to enlarged (possible fused) facets. Cells of all types (pigment, cone, photoreceptor) appear to be randomly missing from ommatidial arrays. The second mitotic wave proceeds normally in pieEB3
homozygous eye discs, but unlike wild-type abundant apoptotic bodies are evident both anterior and posterior to the morphogenetic furrow. Excess cell death is also seen in wing and leg imaginal discs in these flies. On average pieEB3
homozygous adult emerge 2.5 days later than their pieEB3
/+ siblings. Homozygotes comprise 23% of the adult population instead of the expected 50%. pieEB3
is semi-lethal over Df(2L)J2
. Those surviving to adulthood are very sickly, females are infertile, or nearly so, and males have reduced fertility. Somatic clones of pieEB3
cells in the adult eye have almost normal morphology. However, pieEB3
somatic clones in the eye disc have increased cell death.
Most ommatidia contain normal array of retinula cells, photoreceptor cells are sometimes directly adjacent to neighbouring units. Associated sets of photoreceptor cells occupy a single enlarged facet and are jointly surrounded by pigment cells. Homozygotes have heldout notched wings. Eye discs sometimes show irregular spacing of the ommatidia in homozygotes. Homozygous adults have a rough eye phenotype.