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General Information
Symbol
Dmel\pieEB3
Species
D. melanogaster
Name
FlyBase ID
FBal0032439
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:
C10421406T
Reported nucleotide change:
C?T
Amino acid change:
Q391term | pie-PA; Q391term | pie-PB
Reported amino acid change:
Q391term
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Amino acid replacement: Q391term.
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
pieE1-16/pieEB3 mutant adult survivors exhibit a marked rough eye phenotype.
Homozygous pieEB3 flies have rough eyes. Facet size varies from smaller than normal to enlarged (possible fused) facets. Cells of all types (pigment, cone, photoreceptor) appear to be randomly missing from ommatidial arrays. The second mitotic wave proceeds normally in pieEB3 homozygous eye discs, but unlike wild-type abundant apoptotic bodies are evident both anterior and posterior to the morphogenetic furrow. Excess cell death is also seen in wing and leg imaginal discs in these flies. On average pieEB3 homozygous adult emerge 2.5 days later than their pieEB3/+ siblings. Homozygotes comprise 23% of the adult population instead of the expected 50%. pieEB3 is semi-lethal over Df(2L)J2, Df(2L)J27, Df(2L)J39, Df(2L)J106 or Df(2L)J77. Those surviving to adulthood are very sickly, females are infertile, or nearly so, and males have reduced fertility. Somatic clones of pieEB3 cells in the adult eye have almost normal morphology. However, pieEB3 somatic clones in the eye disc have increased cell death.
Most ommatidia contain normal array of retinula cells, photoreceptor cells are sometimes directly adjacent to neighbouring units. Associated sets of photoreceptor cells occupy a single enlarged facet and are jointly surrounded by pigment cells. Homozygotes have heldout notched wings. Eye discs sometimes show irregular spacing of the ommatidia in homozygotes. Homozygous adults have a rough eye phenotype.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressed by
Statement
Reference
Suppressor of
Statement
Reference
pieEB3 is a suppressor of visible phenotype of upd1GMR.PB
Other
Statement
Reference
Phenotype Manifest In
Suppressed by
Statement
Reference
pieEB3 has eye phenotype, suppressible | partially by BacA\p35GMR.PH
Suppressor of
Statement
Reference
pieEB3 is a suppressor of eye phenotype of upd1GMR.PB
Additional Comments
Genetic Interactions
Statement
Reference
In pieEB3 homozygotes the cell death increase posterior to the morphogenetic furrow in eye discs is suppressed and the rough eye phenotype of adults is mostly suppressed by BacA\p35GMR.PH. pieEB3/+ is lethal in combination with Wunspecified/+.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
Name Synonyms
Secondary FlyBase IDs
    References (4)