FlyBase Allele Report: Dmel\puc[A251.1F3]

General Information

Symbol

Dmel\puc^A251.1F3

Species

D. melanogaster

Name

FlyBase ID

FBal0032523

Feature type

allele

Associated gene

Dmel\puc

Associated Insertion(s)

P{lArB}puc^A251.1F3

Carried in Construct

Also Known As

puc-lacZ, puc^A251, puc^A251.1, puc^A251-lacZ

Key Links

Allele class

Mutagen

P-element activity

Nature of the Allele

Allele class

Mutagen

P-element activity

(Peifer, 1997.2.10)

Progenitor genotype

Associated Insertion(s)

P{lArB}puc^A251.1F3

Cytology

Description

P{lArB} insertion in intron 2.

(Dobens et al., 2001)

P{lArB} insertion in the second intron.

(Martin-Blanco et al., 1998)

Allele components

Component

Use(s)

Inserted element

P{lArB}

enhancer trap

Encoded product / tool

lacZ

reporter enzyme

Tagged with

Tag:NLS(P)

nuclear protein localization tag

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

Phenotype Manifest In

collar | maternal effect | somatic clone

(Dobens et al., 2001)

dorsal appendage | maternal effect | somatic clone

(Dobens et al., 2001)

egg | maternal effect | somatic clone

(Dobens et al., 2001)

egg chorion | maternal effect | somatic clone

(Dobens et al., 2001)

egg operculum | maternal effect | somatic clone

(Dobens et al., 2001)

embryonic/first instar larval cuticle | dorsal

(McEwen et al., 2000)

embryonic/first instar larval cuticle | maternal effect

(McEwen and Peifer, 2005)

embryonic epidermis | dorsal

(McEwen et al., 2000)

embryonic segment | maternal effect

(McEwen and Peifer, 2005)

eye

(McEwen and Peifer, 2005)

follicle cell | somatic clone

(Dobens et al., 2001)

larval dorsal hair

(McEwen et al., 2000)

nucleus & follicle cell | somatic clone

(Jordan et al., 2006)

nurse cell | maternal effect | somatic clone

(Dobens et al., 2001)

ventral denticle belt

(McEwen et al., 2000)

ventral denticle belt | maternal effect

(McEwen and Peifer, 2005)

Detailed Description

Statement

Reference

Follicle cells in puc^A251.1F3 clones show no consistent defect in mitotic cycles between stages 1-6 in oogenesis. However the nuclei in puc^A251.1F3 mutant cells remain small, suggesting that these follicle cells do not enter endocycles at the mitotic-to-endocycle transition.

(Jordan et al., 2006)

Mothers with germlines homozygous for puc^A251.1F3 lay reduced numbers of eggs; these eggs are small relative to wild type. Resulting puc^A251.1F3 maternal/zygotic embryos have severe defects in embryonic development and secrete only fragmented scraps of cuticle. The epithelial cells of these embryos undergo an increase in apoptosis in a segmentally repeated pattern in both the ventral and lateral epidermis. The cuticle defects are suppressed in embryos with a paternal wild-type copy of puc, but this fails to rescue embryonic lethality. puc^A251.1F3 maternal-only embryos display patterning defects, exhibiting segmental deletions and fusions and a corresponding loss of denticle belts.

puc^A251.1F3 mutant clones cannot be recovered in the wing disc.

The eye tissue of puc^A251.1F3 homozygous flies is almost completely missing.

(McEwen and Peifer, 2005)

In contrast to wild-type flies, puc^A251.1F3/+ flies do not die in response to being fed with paraquat. puc^A251.1F3/+ males show significant extension of mean and maximal lifespan when compared to wild-type flies. Females show qualitatively similar lifespan data, although the extension of lifespan is less pronounced than in males. puc^A251.1F3/+ flies contain significantly decreased levels of oxidized proteins compared to wild-type flies.

(Wang et al., 2003)

Females containing homozygous clones produce small, unfertilised eggs with aberrant eggshells 48 hours after clone induction. The eggshells are open in the anterior, with inadequate sealing between the operculum and the collar and the dorsal appendages are shorter than normal and variable in width. Egg chambers containing chorion encased small eggs have a mass of nurse cells similar in size to stage 11 nurse cells, indicating that nurse cell dumping is not completed. Eggs derived from females containing homozygous germline clones have normal eggshells and are fertilised. Homozygous anterior follicle cell clones are associated with defects in dorsal appendage formation. At late stages aberrant cell shapes and defects in epithelial continuity are seen in main body follicle cell clones. This class includes flaccid cup-shaped eggs that are likely resorbed and not laid.

(Dobens et al., 2001)

Embryos show puckering of dorsal epidermis with loss of dorsal hair identity and a naked scar at the dorsal midline. Ventrally, denticle belts are narrowed, with the narrowing most pronounced at the ventral midline.

(McEwen et al., 2000)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by bsk¹/bsk[+]

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by zip[+]/zip¹

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype, suppressible by sqh^E20.E21

(Kirchner et al., 2007)

NOT suppressed by

Statement

Reference

gang-of-four^x, puc^A251.1F3/puc[+] has lethal | dominant phenotype, non-suppressible by Df(3L)H99/+

(Beam and Moberg, 2010)

flw¹, puc^A251.1F3/puc[+] has visible phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Kirchner et al., 2007)

NOT Enhancer of

Statement

Reference

puc^A251.1F3/puc[+] is a non-enhancer of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

puc^A251.1F3/puc[+] is a non-enhancer of lethal | embryonic stage | recessive | partially phenotype of peb³⁰⁸

(Wilk et al., 2004)

Suppressor of

Statement

Reference

puc^A251.1F3/puc[+] is a suppressor of visible | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

NOT Suppressor of

Statement

Reference

puc^A251.1F3/puc[+] is a non-suppressor of visible | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

puc^A251.1F3/puc[+] is a non-suppressor of lethal | recessive | partially | embryonic stage phenotype of peb³⁰⁸

(Wilk et al., 2004)

Other

Statement

Reference

bru3⁰⁵⁸⁷¹/l(3)05871[+], puc^A251.1F3 has viable phenotype

(Beam and Moberg, 2010)

gang-of-four¹, puc^A251.1F3/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

gang-of-four², puc^A251.1F3/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

gang-of-four^x, puc^A251.1F3/puc[+] has lethal | dominant phenotype

(Beam and Moberg, 2010)

flw¹, puc^A251.1F3/puc[+] has viable phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has visible phenotype

(Kirchner et al., 2007)

Mbs³/Mbs[+], flw¹, puc^A251.1F3/puc[+] has lethal phenotype

(Kirchner et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, puc^A251.1F3/puc[+] has increased cell number phenotype

(McEwen and Peifer, 2005)

Phenotype Manifest In

Suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by bsk¹/bsk[+]

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by zip[+]/zip¹

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has wing phenotype, suppressible by sqh^E20.E21

(Kirchner et al., 2007)

puc^A251.1F3 has eye phenotype, suppressible by bsk²/bsk[+]

(McEwen and Peifer, 2005)

NOT suppressed by

Statement

Reference

flw¹, puc^A251.1F3/puc[+] has wing phenotype, non-suppressible by sqh^{A20.A21.Tag:FLAG}

(Kirchner et al., 2007)

Enhancer of

Statement

Reference

puc^A251.1F3/puc[+] is an enhancer of eye phenotype of hep^{CA.sev.Tag:MYC}

(Baril et al., 2009)

puc^A251.1F3/puc[+] is an enhancer of eye phenotype of Rac1^GMR.PN

(Baril et al., 2009)

puc^A251.1F3/puc^A251.1F3 is an enhancer of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

puc^A251.1F3 is an enhancer of embryonic epidermis | dorsal phenotype of wg^l-8

(McEwen et al., 2000)

puc^A251.1F3 is an enhancer of embryonic/first instar larval cuticle | dorsal phenotype of wg^l-8

(McEwen et al., 2000)

NOT Enhancer of

Statement

Reference

puc^A251.1F3/puc[+] is a non-enhancer of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

puc^A251.1F3/puc[+] is a non-enhancer of wing phenotype of pcm⁵

(Grima et al., 2008)

Suppressor of

Statement

Reference

puc^A251.1F3/puc[+] is a suppressor of eye | heat sensitive phenotype of peb¹

(Wilk et al., 2004)

puc^A251.1F3 is a suppressor of phenotype of arm³

(Cox et al., 2000)

puc^A251.1F3 is a suppressor | partially of ventral denticle belt phenotype of wg^PE4/Df(2L)DE

(McEwen et al., 2000)

puc^A251.1F3 is a suppressor | partially of embryonic epidermis | ventral phenotype of wg^PE4/Df(2L)DE

(McEwen et al., 2000)

puc^A251.1F3/puc[+] is a suppressor of ventral denticle belt phenotype of arm³

(McEwen et al., 2000)

puc^A251.1F3/puc[+] is a suppressor of embryonic epidermis | dorsal phenotype of arm³

(McEwen et al., 2000)

puc^A251.1F3/puc[+] is a suppressor of embryonic epidermis | ventral phenotype of arm³

(McEwen et al., 2000)

puc^A251.1F3 is a suppressor of ventral denticle belt phenotype of pan²

(McEwen et al., 2000)

puc^A251.1F3 is a suppressor of embryonic epidermis | ventral phenotype of pan²

(McEwen et al., 2000)

puc^A251.1F3 is a suppressor of embryonic/first instar larval cuticle | dorsal phenotype of hep¹

(Martin-Blanco et al., 1998)

NOT Suppressor of

Statement

Reference

puc^A251.1F3/puc[+] is a non-suppressor of eye | adult stage phenotype of Magi^UAS.GFP, Scer\GAL4^GMR.PU

(Zaessinger et al., 2015)

puc^A251.1F3 is a non-suppressor of ventral denticle belt phenotype of wg^l-8

(McEwen et al., 2000)

puc^A251.1F3 is a non-suppressor of embryonic epidermis | ventral phenotype of wg^l-8

(McEwen et al., 2000)

Other

Statement

Reference

pcm⁵, puc^A251.1F3/puc[+] has adult thorax phenotype

(Grima et al., 2008)

flw¹, puc^A251.1F3/puc[+] has wing phenotype

(Kirchner et al., 2007)

flw¹, puc^A251.1F3/puc[+] has metathoracic leg phenotype

(Kirchner et al., 2007)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, puc^A251.1F3/puc[+] has wing phenotype

(McEwen and Peifer, 2005)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, puc^A251.1F3/puc[+] has leg phenotype

(McEwen and Peifer, 2005)

BacA\p35^UAS.cHa, Scer\GAL4^en-e16E, puc^A251.1F3/puc[+] has wing disc phenotype

(McEwen and Peifer, 2005)

arm³, puc^A251.1F3/puc[+] has embryonic epidermis | dorsal phenotype

(McEwen et al., 2000)

pan², puc^A251.1F3 has embryonic epidermis | dorsal phenotype

(McEwen et al., 2000)

arm³, puc^A251.1F3 has embryonic epidermis | dorsal phenotype

(McEwen et al., 2000)

puc^A251.1F3, wg^PE4 has embryonic epidermis | dorsal phenotype

(McEwen et al., 2000)

Additional Comments

Genetic Interactions

Statement

Reference

The rough eye phenotype observed in adults expressing Magi^{Scer\UAS.T:Avic\GFP} under the control of Scer\GAL4^GMR.PU is not modified by combination with single copy of puc^A251.1F3.

(Zaessinger et al., 2015)

puc^A251.1F3 dominantly suppresses the wing notching phenotype seen in Df(1)N-54l9 heterozygotes (the penetrance of the phenotype is significantly decreased in the double heterozygotes).

(Beam and Moberg, 2010)

Male and female flies homozygous (or hemizygous) for pcm⁵ and heterozygous for puc^A251.1F3 are viable, but over half show a bald patch at the anterior end of the dorsal midline at 19[o]C, indicating that thorax closure is not quite complete. At 25[o]C the majority of flies have bald patches.

Male and female flies homozygous (or hemizygous) for pcm⁵ and heterozygous for puc^A251.1F3 have dull wings at 19[o]C and 25[o]C.

(Grima et al., 2008)

The flw¹/Y ; puc^A251.1F3/+ combination is viable. 30% of these flies have wing phenotypes that range from slight defects in the posterior part of the wing to stumps. Flies with the most severe wing phenotypes sometimes have third leg malformations.

Pp1-87B^87Bg-3 does not show any discernible mutant phenotype with puc^A251.1F3.

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are suppressed to wild type by bsk¹/+, zip¹/+ or sqh^E20.E21.

The wing defects seen in flw¹/Y ; puc^A251.1F3/+ flies are not suppressed by sqh^{A20.A21.T:Zzzz\FLAG}.

(Kirchner et al., 2007)

Heterozygosity for bsk² partially rescues puc^A251.1F3 mutant eye tissue.

(McEwen and Peifer, 2005)

arm³,puc^A251.1F3, pan²,puc^A251.1F3, wg^PE4,puc^A251.1F3 and wg^l-8,puc^A251.1F3 double mutants all show a novel loss of dorsal cuticle phenotype.

(McEwen et al., 2000)

Dominantly partially suppresses the dorsal open phenotype of hep¹ hemizygous embryos.

(Martin-Blanco et al., 1998)

Xenogenetic Interactions

Statement

Reference

Expression of BacA\p35^Scer\UAS.cHa, driven by Scer\GAL4^en-e16E, in a puc^A251.1F3/+ background causes tissue overgrowth throughout the posterior compartment of the wing disc in >90% of flies. Disrupted foci within the disc contain excess cells, resulting in abnormal folding of the disc epithelium. Most cells within these foci are not apoptotic. Some discs show extensive areas of overgrowth. In adults, overgrowths in the interior of the wing resemble wing blisters; excess cells appear to be in one compartment as overgrowths project from either the dorsal or the ventral surfaces. When overgrowths arise along the wing margin, excess tissue is readily apparent as an extension of the wing. Similar but less frequent outgrowths occur in posterior regions of adult legs.

(McEwen and Peifer, 2005)

Complementation and Rescue Data

Fails to complement

puc^JK0

(Kirchner et al., 2007)

puc^67BDn

(Kirchner et al., 2007)

puc^j4E1

(Kirchner et al., 2007)

puc^S023803

(Prokopenko et al., 2000)

puc^E69

(Martin-Blanco et al., 1998)

Partially rescued by

puc^A251.1F3 is partially rescued by Scer\GAL4^Act5C.PI/puc^UASp.Tag:MYC

(McEwen and Peifer, 2005)

Comments

Expression of puc^{Scer\UAS.P\T.T:Hsap\MYC} at the time of clone induction (driven by Scer\GAL4^Act5C.PI) rescues puc^A251.1F3 cell viability in the wing disc, although fewer puc^A251.1F3 clones are recovered compared to wild-type clones.

(McEwen and Peifer, 2005)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

11173

P{lArB}puc^A251.1F3 ry⁵⁰⁶/TM3, Sb¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (5)

Reported As

Symbol Synonym

l(3)A251.1

(Cox et al., 2000)

puc^A251.1

(Wang et al., 2003, McEwen et al., 2000, Martin-Blanco et al., 1998)

puc^A251.1F3

(Zhu et al., 2019, Zaessinger et al., 2015, Baril et al., 2009, Neuman-Silberberg, 2007)

puc^A251.1f3

(Wilk et al., 2004)

puc^A251

(Klemm et al., 2021, Beam and Moberg, 2010, Grima et al., 2008, Kirchner et al., 2007, Jordan et al., 2006, Dobens et al., 2001)

Name Synonyms

Secondary FlyBase IDs

References (19)

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