FlyBase Allele Report: Dmel\pum[Msc]

General Information

Symbol

Dmel\pum^Msc

Species

D. melanogaster

Name

FlyBase ID

FBal0032526

Feature type

allele

Associated gene

Dmel\pum

Associated Insertion(s)

Carried in Construct

Key Links

Allele class

Mutagen

Nature of the Allele

Allele class

Mutagen

Progenitor genotype

Caused by aberration

In(3R)Scr^Msc

Cytology

Description

Inversion breakpoint lies within the pumilio protein coding region.

(Barker et al., 1992)

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal neuroanatomy (with pum⁷)

(Menon et al., 2004)

abnormal neurophysiology (with pum⁷)

(Menon et al., 2004)

lethal | maternal effect | recessive

(Barker et al., 1992)

visible (with pum¹)

(Kim et al., 2012)

visible (with pum³)

(Kim et al., 2012)

visible (with pum⁰¹⁶⁸⁸)

(Kim et al., 2012)

Phenotype Manifest In

embryonic/larval neuromuscular junction (with pum⁷)

(Menon et al., 2004)

embryonic abdomen (with pum⁶⁸⁰)

(Sonoda and Wharton, 2001)

embryonic abdominal segment

(Wharton et al., 1998)

embryonic abdominal segment (with pum⁶⁸⁰)

(Sonoda and Wharton, 2001)

embryonic epidermis (with pum⁶⁸⁰)

(Sonoda and Wharton, 2001)

macrochaeta | ectopic (with pum¹)

(Kim et al., 2012)

macrochaeta | ectopic (with pum³)

(Kim et al., 2012)

metanotum (with pum¹)

(Kim et al., 2012)

metanotum (with pum³)

(Kim et al., 2012)

NMJ bouton (with pum⁷)

(Menon et al., 2004)

primordial germ cell | maternal effect (with pum^FC8)

(Asaoka-Taguchi et al., 1999)

wing vein | ectopic (with pum¹)

(Kim et al., 2012)

wing vein | ectopic (with pum³)

(Kim et al., 2012)

wing vein | ectopic (with pum⁰¹⁶⁸⁸)

(Kim et al., 2012)

Detailed Description

Statement

Reference

Trans-heterozygotes of pum¹/pum^Msc display extra wing veins and extra macrochaetae on their notum. The penetrance of the phenotypes is incomplete.

Trans-heterozygotes of pum³/pum^Msc display extra wing veins and extra macrochaetae on their notum. The penetrance of the phenotypes is incomplete.

Trans-heterozygotes of pum^Msc/pum⁰¹⁶⁸⁸ display extra wing veins and extra macrochaetae on their notum. The penetrance of the notum phenotype is incomplete.

(Kim et al., 2012)

pum^Msc/pum⁷ mutant muscle 12 1b neuromuscular junctions differ in a number of ways from wild-type. The synaptic span (the largest distance between the terminal boutons of the 1b synapse) is much smaller in these mutants, abnormally large boutons are found, and the total number of 1b boutons is reduced, while the average area of a type 1b bouton is increased.

An increase in mEJP amplitudes is not found in pum^Msc/pum⁷ mutant larvae.

mEJP frequency is elevated by 1.8- to 3-fold relative to heterozygote and wild-type controls in pum⁷/pum^Msc mutants.

pum^Msc/pum⁷ larval muscle 6/7 neuromuscular junctions in segments in A2-A5 exhibit an increase in eIF-4E aggregates from a wild-type average of 4.8 to approximately 18 per neuromuscular junction.

(Menon et al., 2004)

Most embryos laid by pum^Msc/pum¹³ mothers have 2 or 3 abdominal segments rather than the normal 8.

(Sonoda and Wharton, 2001)

Pole cells of embryos derived from pum^Msc/pum^FC8 females transplanted into wild-type host embryos pass normally through the midgut epithelium into the haemocoel. However, none of the transplanted pole cells are incorporated into the gonads of the host embryos, and they remain in the haemocoel and gut lumen. The average number of pole cells per embryo is reduced compared to controls in embryos derived from pum^Msc/pum^FC8 females.

(Asaoka-Taguchi et al., 1999)

Embryos derived from pum^Msc/pum^FC8 embryos have no abdominal segments. This phenotype is fully rescued if the females carry pum^nos.PB, and is largely rescued if the females carry pum^Δ2'.nos or pum^Δ5'.nos.

(Wharton et al., 1998)

Embryos from homozygous females fail to develop abdominal segments at any temperature. Injection of nanos RNA does not restore abdominal segmentation.

(Barker et al., 1992)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhancer of

Statement

Reference

pum^Msc/pum[+] is an enhancer of visible phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

NOT Enhancer of

Statement

Reference

pum^Msc/pum[+] is a non-enhancer of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

NOT Suppressor of

Statement

Reference

pum^Msc/pum[+] is a non-suppressor of visible phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Phenotype Manifest In

NOT suppressed by

Statement

Reference

pum^Msc/pum¹ has ovary phenotype, non-suppressible by cup^unspecified

(Verrotti and Wharton, 2000)

pum^Msc/pum^FC8 has ovary phenotype, non-suppressible by cup^unspecified

(Verrotti and Wharton, 2000)

Enhancer of

Statement

Reference

pum^Msc/pum[+] is an enhancer of female germline stem cell phenotype of twin^DG24102

(Joly et al., 2013)

pum^Msc/pum[+] is an enhancer of metanotum phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

pum^Msc/pum[+] is an enhancer of macrochaeta | ectopic phenotype of Egfr^UAS.cUa, Scer\GAL4^sca-537.4

(Kim et al., 2012)

NOT Enhancer of

Statement

Reference

pum^Msc/pum[+] is a non-enhancer of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

NOT Suppressor of

Statement

Reference

pum^Msc/pum[+] is a non-suppressor of wing phenotype of Hsap\PABPN1^17ala.Act88F

(Chartier et al., 2015)

Additional Comments

Genetic Interactions

Statement

Reference

The loss of germline stem cells seen in the ovaries of homozygous twin^DG24102 females is accelerated if the females are also heterozygous for pum^Msc.

(Joly et al., 2013)

Heterozygous pum^Msc greatly enhances the ectopic macrochaeta phenotype induced by Egfr^Scer\UAS.cUa overexpression in the notum under the control of Scer\GAL4^sca-537.4.

(Kim et al., 2012)

cup^unspecified has no effect on the oogenesis defects associated with pum¹/pum^Msc or pum^FC8/pum^Msc females.

(Verrotti and Wharton, 2000)

Xenogenetic Interactions

Statement

Reference

pum^Msc does not alter the wing posture defects seen upon expression of Hsap\PABPN1^17ala.Act88F.

(Chartier et al., 2015)

Complementation and Rescue Data

Partially complements

pum⁰¹⁶⁸⁸

(Parisi and Lin, 1999)

Rescued by

pum^Msc/pum^FC8 is rescued by pum^nanos.PB

(Wharton et al., 1998)

Partially rescued by

pum^Msc/pum^FC8 is partially rescued by pum^Δ2'.C1365R

(Sonoda and Wharton, 2001)

pum^Msc/pum^FC8 is partially rescued by pum^Δ5'.nanos

(Wharton et al., 1998)

pum^Msc/pum^FC8 is partially rescued by pum^Δ2'.nanos

(Wharton et al., 1998)

Not rescued by

pum^Msc/pum^FC8 is not rescued by pum^Δ2'.T1366D

(Sonoda and Wharton, 2001)

Comments

pum⁰¹⁶⁸⁸ partially complements the embryonic lethality of the maternal effect allele pum^Msc, resulting in viable yet sterile progeny.

(Parisi and Lin, 1999)

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

2186

In(3R)Scr^Msc, ru¹ Scr^Msc pum^Msc ss¹ e^s/TM3, Ser¹

Kyoto

106733

In(3R)Scr^Msc, ru¹ Scr^Msc pum^Msc ss¹ e^s/TM3, Ser¹

Notes on Origin

Discoverer

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

Msc

(Tadros et al., 2007)

pum^MSC

(Wharton et al., 2018, Joly et al., 2013, Chen and McKearin, 2005, Chagnovich and Lehmann, 2001, Chagnovich and Lehmann, 2001, Parisi and Lin, 1999)

pum^Msc

(Ribot et al., 2022, Eichler et al., 2020, Chartier et al., 2015, Kim et al., 2012, Harris et al., 2011, Kadyrova et al., 2007, Menon et al., 2004)

pum^msc

(Miles et al., 2014)

Name Synonyms

Secondary FlyBase IDs

References (25)

Report Sections

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