FlyBase Allele Report: Dmel\robo1[2]

General Information

Symbol

Dmel\robo1²

Species

D. melanogaster

Name

FlyBase ID

FBal0032589

Feature type

allele

Associated gene

Dmel\robo1

Associated Insertion(s)

Carried in Construct

Also Known As

robo²

Key Links

Allele class

amorphic allele - genetic evidence

Mutagen

ethyl methanesulfonate

Nature of the Allele

Allele class

amorphic allele - genetic evidence

(Kidd et al., 1998)

Mutagen

ethyl methanesulfonate

(Kidd et al., 1998, Kolodziej et al., 1995, Seeger et al., 1993)

Progenitor genotype

Cytology

Description

Mutations Mapped to the Genome

Curation Data

Type

Location

Additional Notes

References

Variant Molecular Consequences

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Disease-implicated variant(s)

Phenotypic Data

Phenotypic Class

abnormal locomotor behavior | dominant | temperature conditional

(Kucherenko et al., 2011)

abnormal neuroanatomy (with robo1¹)

(Mauss et al., 2009)

abnormal neuroanatomy | embryonic stage

(Lee et al., 2007)

Phenotype Manifest In

axon & pCC neuron

(Lee et al., 2007)

chordotonal organ & axon | lateral

(Kolodziej et al., 1995)

filopodium | embryonic stage

(Murray and Whitington, 1999)

germline cell | embryonic stage (with robo1¹)

(Weyers et al., 2011)

gonad | embryonic stage (with robo1¹)

(Weyers et al., 2011)

interneuron | embryonic stage

(Murray and Whitington, 1999)

larval RP2 motor neuron

(Murray and Whitington, 1999)

motor neuron (with robo1¹)

(Mauss et al., 2009)

pCC neuron

(Murray and Whitington, 1999)

Detailed Description

Statement

Reference

robo1²/+ mutant flies exhibit temperature-induced mobility defects.

(Kucherenko et al., 2011)

robo¹/robo² embryos exhibit gonad compaction defects and germ cell ensheathment defects.

(Weyers et al., 2011)

robo¹/robo² mutants exhibit an ectopic midline innervation phenotype (with 100% penetrance for MN-LL1 and 89% penetrance for MN-DA3).

(Mauss et al., 2009)

In robo² embryos, the pCC axon rarely aberrantly crosses the midline.

(Lee et al., 2007)

As well as the interneurons, RP2 (normally an ipsilaterally projecting neuron) can project contralaterally. RP2 and pCC can bifurcate into ipsilateral and contralateral branches. RP2 can be misrouted posteriorly. Time lapse photography shows filopodia projecting across the midline for longer than in wild type. Longer filopodia are not restricted to pathway directions. These filopodia can lead to branched growth cones. Filopodial number is not affected. Heterozygotes show a filopodial length distribution that is intermediate between wild type and homozygous mutants.

(Murray and Whitington, 1999)

Mutant phenotype of lateral chordotonal axons includes: shorter axons or defasciculated axons.

(Kolodziej et al., 1995)

"Fuzzy commisure" phenotype in embryonic central nervous system.

(Seeger et al., 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Enhanced by

Statement

Reference

robo1² has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

robo1² has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by shot³

(Lee et al., 2007)

robo1², shot³ has abnormal neuroanatomy | embryonic stage phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

Enhancer of

Statement

Reference

robo1²/robo1[+] is an enhancer of visible phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Suppressor of

Statement

Reference

robo1²/robo1[+] is a suppressor of visible phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

robo1²/robo1[+] is a suppressor of visible phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Sac1¹/Sac1[+], robo1² has abnormal neuroanatomy | dominant | embryonic stage phenotype

(Lee et al., 2011)

Phenotype Manifest In

Enhanced by

Statement

Reference

robo1² has axon & pCC neuron phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

robo1² has axon & pCC neuron phenotype, enhanceable by shot³

(Lee et al., 2007)

robo1², shot³ has axon & pCC neuron phenotype, enhanceable by exba[+]/kra²

(Lee et al., 2007)

NOT Enhanced by

Statement

Reference

robo1²/robo1¹ has gonad | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ has gonad | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

robo1²/robo1¹ has germline cell | embryonic stage phenotype, non-enhanceable by robo3¹/robo3¹

(Weyers et al., 2011)

Enhancer of

Statement

Reference

robo1²/robo1[+] is an enhancer of posterior crossvein phenotype of Dg^RNAi.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

NOT Enhancer of

Statement

Reference

robo1²/robo1¹ is a non-enhancer of gonad | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of gonadal sheath proper primordium | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of germline cell | embryonic stage phenotype of robo2⁴/robo2⁸

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of gonad | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

robo1²/robo1¹ is a non-enhancer of germline cell | embryonic stage phenotype of Df(2L)Exel7006/robo3¹

(Weyers et al., 2011)

Suppressor of

Statement

Reference

robo1²/robo1[+] is a suppressor of indirect flight muscle cell phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

robo1²/robo1[+] is a suppressor of indirect flight muscle cell phenotype of Dg^RNAi.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2011)

robo1²/robo1[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.NH2.UAS, Scer\GAL4^Act.PU

(Kucherenko et al., 2008)

robo1²/robo1[+] is a suppressor of posterior crossvein phenotype of Dys^RNAi.C.UAS, Scer\GAL4^Tub.PU

(Kucherenko et al., 2008)

Other

Statement

Reference

Dys^RNAi.C.UAS, robo1²/robo1[+] has wing vein | ectopic phenotype

(Kucherenko et al., 2008)

Additional Comments

Genetic Interactions

Statement

Reference

robo1 Dys double heterozygous flies (robo1²/Df(3R)Exel6184) do not exhibit indirect flight muscle degeneration.

robo1² Dg^O86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of robo1² suppresses the indirect flight muscle degeneration seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of robo1² suppresses the indirect flight muscle degeneration seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2011)

robo²/+ Sac1¹/+ double heterozygotes show a high frequency of ectopic midline crossings in the central nervous system.

(Lee et al., 2011)

Combination of either Dg^O86 or Df(3R)Exel6184 in heterozygous state with a single copy of robo1² does not significantly affect the frequency of lamina plexus defects in the third instar larvae.

(Marrone et al., 2011)

One copy of robo1² weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.NH2.Scer\UAS} is expressed under the control of Scer\GAL4^Act.PU.

One copy of robo1² weakly suppresses the detached posterior crossvein phenotype seen when Dys^{dsRNA.C.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU but produces extra wing vein material.

One copy of robo1² enhances the posterior crossvein phenotype seen when Dg^{dsRNA.Scer\UAS} is expressed under the control of Scer\GAL4^tub.PU.

(Kucherenko et al., 2008)

The pCC axon midline crossing phenotype of robo² embryos is dramatically enhanced in robo²/+; exba²/+ embryos and in shot³, robo²/+ embryos. The phenotype is further enhanced in shot³, robo²/+; exba²/+ embryos.

(Lee et al., 2007)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Rescued by

robo1²/robo1¹ is rescued by robo1^UAS.cKa/Scer\GAL4^eve.CQ2

(Mauss et al., 2009)

Comments

The ectopic midline innervation phenotype found in robo¹/robo² mutants can be rescued by expression of robo^Scer\UAS.cKa under the control of Scer\GAL4^eve.CQ2.

(Mauss et al., 2009)

Images (0)

Mutant

Wild-type

Stocks (0)

Notes on Origin

Discoverer

Induced on: Fas3 null chromosome.

(Kidd et al., 1998)

Comments

The 'robo1[2]' stock previously held at the Bloomington Drosophila Stock Center (number 8756) was found to contain the same mutation as the distinct and independent 'robo1[1]' allele, suggesting that the allele in the stock labeled as 'robo1[2]' was mis-identified. Stock number 8756 has since been discarded.

(Bosch, 2014.11.5)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

GA1112

(Kidd et al., 1998)

robo1²

(Huang et al., 2022)

robo²

(Sarov et al., 2016, Bosch, 2014.11.5, Knox et al., 2011, Kucherenko et al., 2011, Lee et al., 2011, Marrone et al., 2011, Weyers et al., 2011, Kucherenko et al., 2008, Lee et al., 2007)

robo^GA1112

(Mauss et al., 2009)

Name Synonyms

Secondary FlyBase IDs

References (15)

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