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General Information
Symbol
Dmel\robo12
Species
D. melanogaster
Name
FlyBase ID
FBal0032589
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
robo2
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

robo12/+ mutant flies exhibit temperature-induced mobility defects.

robo1/robo2 embryos exhibit gonad compaction defects and germ cell ensheathment defects.

robo1/robo2 mutants exhibit an ectopic midline innervation phenotype (with 100% penetrance for MN-LL1 and 89% penetrance for MN-DA3).

In robo2 embryos, the pCC axon rarely aberrantly crosses the midline.

As well as the interneurons, RP2 (normally an ipsilaterally projecting neuron) can project contralaterally. RP2 and pCC can bifurcate into ipsilateral and contralateral branches. RP2 can be misrouted posteriorly. Time lapse photography shows filopodia projecting across the midline for longer than in wild type. Longer filopodia are not restricted to pathway directions. These filopodia can lead to branched growth cones. Filopodial number is not affected. Heterozygotes show a filopodial length distribution that is intermediate between wild type and homozygous mutants.

Mutant phenotype of lateral chordotonal axons includes: shorter axons or defasciculated axons.

"Fuzzy commisure" phenotype in embryonic central nervous system.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

robo12 has neuroanatomy defective | embryonic stage phenotype, enhanceable by exba[+]/kra2

robo12, shot3 has neuroanatomy defective | embryonic stage phenotype, enhanceable by exba[+]/kra2

Enhancer of
Statement
Reference

robo12/robo1[+] is an enhancer of visible phenotype of DgdsRNA.UAS, Scer\GAL4Tub.PU

Suppressor of
Statement
Reference

robo12/robo1[+] is a suppressor of visible phenotype of DysdsRNA.NH2.UAS, Scer\GAL4Act.PU

robo12/robo1[+] is a suppressor of visible phenotype of DysdsRNA.C.UAS, Scer\GAL4Tub.PU

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
Statement
Reference

robo12 has axon & pCC neuron phenotype, enhanceable by exba[+]/kra2

robo12 has axon & pCC neuron phenotype, enhanceable by shot3

robo12, shot3 has axon & pCC neuron phenotype, enhanceable by exba[+]/kra2

NOT Enhanced by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference
Other
Statement
Reference
Additional Comments
Genetic Interactions
Statement
Reference

robo1 Dys double heterozygous flies (robo12/Df(3R)Exel6184) do not exhibit indirect flight muscle degeneration.

robo12 DgO86 double heterozygous flies do not exhibit indirect flight muscle degeneration.

One copy of robo12 suppresses the indirect flight muscle degeneration seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of robo12 suppresses the indirect flight muscle degeneration seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

robo2/+ Sac11/+ double heterozygotes show a high frequency of ectopic midline crossings in the central nervous system.

Combination of either DgO86 or Df(3R)Exel6184 in heterozygous state with a single copy of robo12 does not significantly affect the frequency of lamina plexus defects in the third instar larvae.

One copy of robo12 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.NH2.Scer\UAS is expressed under the control of Scer\GAL4Act.PU.

One copy of robo12 weakly suppresses the detached posterior crossvein phenotype seen when DysdsRNA.C.Scer\UAS is expressed under the control of Scer\GAL4tub.PU but produces extra wing vein material.

One copy of robo12 enhances the posterior crossvein phenotype seen when DgdsRNA.Scer\UAS is expressed under the control of Scer\GAL4tub.PU.

The pCC axon midline crossing phenotype of robo2 embryos is dramatically enhanced in robo2/+; exba2/+ embryos and in shot3, robo2/+ embryos. The phenotype is further enhanced in shot3, robo2/+; exba2/+ embryos.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

The ectopic midline innervation phenotype found in robo1/robo2 mutants can be rescued by expression of roboScer\UAS.cKa under the control of Scer\GAL4eve.CQ2.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer

Induced on: Fas3 null chromosome.

Comments
Comments

The 'robo1[2]' stock previously held at the Bloomington Drosophila Stock Center (number 8756) was found to contain the same mutation as the distinct and independent 'robo1[1]' allele, suggesting that the allele in the stock labeled as 'robo1[2]' was mis-identified. Stock number 8756 has since been discarded.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (13)