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General Information
Symbol
Dmel\rut2080
Species
D. melanogaster
Name
FlyBase ID
FBal0032625
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
rutabaga2080, P{lArB}rut2080
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
P{lArB} insertion within 200bp upstream of the presumed transcription start site.
Insertion components
P{lArB}rut2080
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
rut2080 homozygous adults display a significant decrease in intermediate-term (3h after conditioning) olfactory memory, regardless of whether the individuals are subjected or not to cold-shock anesthesia after conditioning, as compared to controls.
rut2080 mutant larvae have a significantly reduced ability to form aversive associative olfactory memories in response to high salt concentration compared to wild-type controls.
As in wild type, rut2080 mutant males exhibit inhibition of female contact-induced male-male aggression seen in wild type males that have previously been exposed to females.
Mutant third instar larvae show a significant decrease in the number of natural synaptopods (motile filopodia-like extensions) at type II neuromuscular junctions compared to wild type, but the number of type II boutons is normal.
rut2080 mutant third instar larval neuromuscular junctions display significantly fewer natural synaptopods than wild-type flies. Application of 10υM octopamine induces synaptopod formation in rut2080 homozygous neuromuscular junctions. rut2080 mutant larvae display normal locomotor activity but exhibit an elimination of the starvation response.
In training trial assays, where flies are repeatedly trained with odor avoidance assays, rut2080 flies perform poorly even after 15 trials, at which point their performance is not significantly different to controls. As early as 30 minutes after training, the memory of rut2080 flies is abolished.
rut2080/Y males show a defect in 2 hour memory compared to wild type.
Mutants show a significant impairment in 3 minute memory after olfactory conditioning compared to wild-type flies.
Homozygous and rut1/rut2080 adults show severely reduced 2 minute memory after a single aversive Pavlovian training session. These adults also show severely reduced memory performance 24 hours after either ten massed or ten spaced sessions of training.
Mutant flies trained by associating an odour with an electric shock do not avoid the odour after training, in contrast to wild-type flies. Mutant flies are defective in action-contingent olfactory learning.
Recently eclosed flies, mutant for rut2080 show increased sleep after social enrichment, although sleep in 3-4 day old adult rut2080 mutants does not respond to changes in the social environment.
rut2080 flies show significantly lower learning after aversive olfactory conditioning (flies trained to associate odors with an electric shock) compared to controls.
Immediate memory after one training session and one-day memory after spaced training is impaired in homozygous flies in a Pavlovian olfactory learning assay.
rut2080 mutants exhibit significantly shorter life spans compared to controls.
Mutant flies show some responsiveness to a novel visual stimulus after a transition (assayed by measuring local field potential activity in the brain), but without the sustained 9-second selection characteristic of wild-type flies, and the mutant flies also respond strongly in the 10- to 20-Hz range. Mutant animals are not distracted by a competing visual stimulus added to an optomotor assay, in contrast to wild-type flies.
rut2080 abolishes the ability of socially enriched adult flies to show decreases in sleep after exposure to social impoverishment.
Mutant flies have normal visual flight control, heat avoidance and pattern discrimination in a visual pattern memory assay, but visual pattern memory is impaired.
rut2080 mutant flies show resistance to V. cholerae infection compared to controls.
rut2080 mutant larvae, trained with LIN/SUC, LIN with DW, or SUC alone fail to exhibit a Response Index (RI) increase, unlike wild-type flies. The gustatory response for SUC is slightly lower in rut2080 larvae than in wild-type flies.
rut2080 flies show a lower calcium response from the dorsal paired neurons than wild-type flies following training consisting of 10s of CS odour, a single electric shock 9s late, 30s fresh air and a further 10s of CS odor.
Shows normal anasthesia resistant memory (ARM).
rut2080 mutants show significantly impaired performance in tests of 3 minute memory after classical conditioning for odors.
When raised at room temperature or at 25oC, the motor axon terminals of rut2080 larvae show a similar level of arborization to wild-type larvae. However, the motor nerve termini of wild-type larvae raised at 30oC show greatly increased levels of branching and variscosities, while no such large increase in terminal projection.
The volume of the antennal lobe and of individual DM2, V and DM6 glomeruli in 6 day old adults is not altered compared to wild type. The response of mutant flies to benzaldehyde is not affected by previous exposure to the odorant. The volume of the antennal lobe neuropile and the DM2, V and DM6 glomeruli is not altered in response to benzaldehyde, in contrast to the adaptive response seen in wild-type flies in response to benzaldehyde.
rut2080 flies have impaired olfactory learning compared to wild-type flies; they are less likely to learn to avoid an odor having experienced it together with an electric shock and are also less likely to be attracted to an odor if it has been combined with a sugar reward. These flies have the same ability as wild type to respond spontaneously to electric shocks, sugar and the odorants EA and IA.
rut2080 mutant flies exhibit memory defects that increase over time, decaying from about 30% of the wild-type level at 3 mins to 20% at 30 mins, and to about zero by 3 hours after training.
Larval molting profile of rut2080/rut2080 and rut2080/rut178 larvae appears normal.
Single-trial short program training in an olfactory learning assay shows that rut2080 flies perform poorly immediately after conditioning. The mutants are sensitive to two-trial, spaced conditioning over two-trial, massed conditioning, behaving in this respect qualitatively like control flies. After mock training, odour avoidance to benzaldehyde or octanol is not significantly different to wild-type flies.
Both training and memory test avoidance performances are lower in rut mutant flies. The test is based on a spatial learning paradigm with a heat punishment procedure. Thermosensitivity of rut2080 flies is indistingushable from that of wild type flies over the temperature ranges employed in the paradigm.
Mutants show defective olfactory short term memory.
Hemizygous males show increased sensitivity to ethanol in an inebriometer assay.
After presentation of electric shock with a first odour, rut2080 flies show a strongly reduced avoidance of a second, different odour compared to wild-type flies.
In an odour avoidance paradigm exhibits significantly reduced learning.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
Suppressed by
Statement
Reference
rut2080 has chemical sensitive phenotype, suppressible by amnchpd
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Other
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Adult males that are both hemizygous for rut2080 and homozygous for Rgk1Δ exhibit a severe decrease in cold-shock anesthesia-resistant intermediate-term olfactory memory (3h after conditioning) as compared to adult males that are only hemizygous for rut2080 or only homozygous for Rgk1Δ.
The reduction in the number of natural synaptopods (motile filopodia-like extensions) at the type II neuromuscular junction which is seen in rut2080 third instar larvae is not altered if the larvae are also mutant for oa21.
Scer\GAL4c747-mediated expression of RdldsRNA.8-10.Scer\UAS does not suppress the low learning performance of rut2080 flies after aversive olfactory conditioning.
amnchpd rut2080 double mutants do not show increased sensitivity to ethanol in an inebriometer assay, although each single mutant does show increased sensitivity to ethanol in an inebriometer assay.
Xenogenetic Interactions
Statement
Reference
Expression of Bper\ptxAact.Scer\UAS for 2 hours at 32[o]C (under the control of both Scer\GAL4Mef2.247 and Scer\GAL80ts.αTub84B) in a rut2080 heterozygous background results in a reduction in learning compared to in a wild-type background, although this is not significant, indicating an additive interaction.
Complementation and Rescue Data
Fails to complement
Rescued by
Comments
Expression of rutScer\UAS.cZa under the control of Scer\GAL4Tdc2.PC rescues the decrease in synaptopods seen at third instar larval neuromuscular junctions in rut2080 mutants. Expression of rutScer\UAS.cZa under the control of Scer\GAL4Tdc2.PC rescues the response to starvation block seen in rut2080 mutants.
Expression of rutScer\UAS.cZa under the control of Scer\GAL4P2.4.Pdf restores the increase in daytime sleep and daytime sleep-bout duration in rut2080 mutants. Expression of rutScer\UAS.cZa under the control of Scer\GAL4elav.Switch.PO restores the increase in daytime sleep and daytime sleep-bout duration in rut2080 mutants.
Expression of rutScer\UAS.cZa is able to suppress the increased resistance to V. cholerae infection seen in rut2080 mutant flies in the absence of a Scer\GAL4 driver.
The short term memory deficit of rut2080 mutants is rescued by rutScer\UAS.cZa; Scer\GAL4Mef2.247.Switch after feeding with 500μM RU486 for 2 days as adults. Shorter RU486 treatments produced incomplete rescue. Animals treated with RU486 during development but not as adults show no rescue 7-10 days after eclosion.
Expression of rutScer\UAS.cZa in a sunset of nerves, under the control of Scer\GAL44G, increases levels of motor axon termini arborization in rut2080 larvae raied at 30oC, but does not increase levels to those seen in wild-type larvae.
Expression of rutScer\UAS.cZa under the control of Scer\GAL4Mef2.247 rescues the limited olfactory learning phenotype of rut2080 mutants.
MB-rescued rut2080 mutant flies (through expression of rutScer\UAS.cZa by Scer\GAL4247) show extinction and extinction control performance indistinguishable from wild-type flies.
Rescue statements based on rescue of the learning and 3 minute memory defect of rut2080 flies. Rescue of rut2080 by Scer\GAL4c522 depends on specific insertion of P{UAS-rut.Z}. Rescue is evident with P{UAS-rut.Z}1 but not P{UAS-rut.Z}2.
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (8)
References (42)