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General Information
Symbol
Dmel\sdETX4
Species
D. melanogaster
Name
FlyBase ID
FBal0032671
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sd-lacZ, sdP(ry+,ETX4), sd-LacZETX4
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Insertion within the first intron.

Insertion of a P{lacZ} element in the first intron, approximately 400bp upstream of the translation start site.

P-element insertion within the first intron.

Insertion components
P{lArB}sdETX4
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

sdETX4 hemizygous males have notched wings.

Hemizygous males have a notched wing phenotype.

Expression of one of sdΔ200.Scer\UAS, sdΔ124-159.Scer\UAS, sdΔ88-159.Scer\UAS, sdΔ160-219.Scer\UAS and sdΔ88-123.Scer\UAS when driven by Scer\GAL4vg.boundary, lead to an enhancement of the wing phenotype seen in sdETX4 mutants. Expression of either sdΔ87.Scer\UAS or sdScer\UAS.cSa driven by Scer\GAL4vg.boundary can either rescue the sdETX4 defective wing phenotype, or can actually make this phenotype more severe, depending on the insertion lines used. The effect of these insertions is also temperature sensitive, with certain insertions suppressing the sdETX4 phenotype at one temperature and enhancing it at another.

sdETX4/Y males display wings with notches at the margin.

Mutants have notches in the wing margin. sd11/sdETX4, sd9/sdETX4 and sd12/sdETX4 flies have a scalloped wing phenotype, with the loss of most of the wing blade tissue and erosion of the wing margin. sd13/sdETX4 flies have a scalloped wing phenotype, with little loss of wing blade tissue but variable erosion of the wing margin (ranging from very conspicuous to loss of only a few margin bristles). sd10/sdETX4 flies have wild-type wings.

Mutant animals have severely notched wings.

Mutant animals have notched wings.

Homozygotes show scalloping of the wing at the anterior margin and at the tip. sdSG29.1/sdETX4 flies show slight nicking of the wing, comparable to that seen in sdSG29.1 homozygotes.

Homozygotes have gaps in the wing margin.

Homozygous females and hemizygous males show nicking of the anterior and lateral margins of the wing blade, with males having a more severe phenotype. sdETX4/Df(1)sd72b flies have vestigial wings and the capitellum is reduced. Approximately 85% of sdETX4 flies lack all wing sensilla. Homozygotes and hemizygotes show a reduced response to 1mM sucrose in a proboscis extension test when stimulated on the tarsal or labellar sensilla. Adults also show a reduced response to sucrose, and an enhanced preference and tolerance for salt in a feeding preference assay. The L1 neuron shows a normal response on stimulation of the labellar taste hairs with varying concentrations of sucrose, NaCl or KCl. Larvae show a strongly reduced avoidance of NaCl and KCl in a chemotaxis test. Adults and larvae show a normal response to benzaldehyde and iso-amyl acetate in olfactory assays.

Mutants show a general taste defect. Wings are scalloped.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

sdETX4 has visible phenotype, enhanceable by yki[+]/ykiB5

sdETX4 has visible phenotype, enhanceable by Df(3R)P-10/+

sdETX4 has visible phenotype, enhanceable by vg[+]/vgnull

sdETX4 has visible phenotype, enhanceable by E2f[+]/E2f191

sdETX4 has visible | recessive phenotype, enhanceable by vg79d5

Suppressor of
Statement
Reference
Other
Phenotype Manifest In
Enhanced by
Statement
Reference

sdETX4 has wing margin phenotype, enhanceable by E-2g[+]/ebi2g2

sdETX4 has wing margin phenotype, enhanceable by ebiE90/ebi[+]

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebiCC3

sdETX4 has wing margin phenotype, enhanceable by E-2g[+]/ebi2g1

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebiCCS-8

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebiE4

sdETX4 has wing margin phenotype, enhanceable by ebiΔ1/ebi[+]

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebiW418X

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebik16213

sdETX4 has wing margin phenotype, enhanceable by ebi[+]/ebiWKS-24

sdETX4 has wing phenotype, enhanceable by yki[+]/ykiB5

sdETX4 has wing margin phenotype, enhanceable by Df(3R)P-10/+

sdETX4 has wing margin phenotype, enhanceable by vg[+]/vgnull

sdETX4 has wing margin phenotype, enhanceable by E2f[+]/E2f191

sdETX4 has wing phenotype, enhanceable by sensLy-1

sdETX4 has wing phenotype, enhanceable by vg79d5/vg1

sdETX4 has wing phenotype, enhanceable by vg79d5

sdETX4 has adult thorax & macrochaeta phenotype, enhanceable by vg79d5

NOT Enhanced by
Statement
Reference

sdETX4 has wing margin phenotype, non-enhanceable by ebi[+]/ebiP7

Suppressed by
NOT suppressed by
Statement
Reference

sdETX4 has wing phenotype, non-suppressible by Fab-71

Enhancer of
Statement
Reference

sdETX4 is an enhancer of wing phenotype of sensLy-1

Suppressor of
Statement
Reference
Other
Additional Comments
Genetic Interactions
Statement
Reference

Heterozygosity for ebi2g2, ebiE90, ebiCC3, ebi2g1, ebiCCS-8, ebiE4, ebiΔ1, ebiW418X, ebik16213 or ebiWKS-24 enhances the notched wing margin phenotype seen in sdETX4 hemizygous males.

ykiB5/+ enhances the notched wing phenotype of sdETX4/Y males.

The defective wing phenotypes seen when sdScer\UAS.cSa or sdΔ200.Scer\UAS are expressed under control of Scer\GAL4vg.boundary in sdETX4 mutants is alleviated when vgScer\UAS.cKa is overexpressed.

sd[ETX4]/Y males display an enhanced wing phenotype in a vgnull/+ background.

sdETX4/Y, E2f91/+ males display a severe mutant wing phenotype compared to sdETX4/Y males.

sdETX4/Y males display an enhanced wing phenotype in Df(3R)P-10/+ background.

The addition of sd::vgScer\UAS.sdTEA (driven by Scer\GAL4vg.PM) to sdETX4 animals, almost completely rescues the wing phenotype with only a small amount of wing notching and occasional loss of bristles on the wing margin. The L5 wing vein is also usually incompletely formed. sdETX4, vg1/vg79d5 wings are very small and deformed. The addition of sd::vgScer\UAS.sdTEA (driven by Scer\GAL4vg.PM) significantly rescues the wing defects.

sdETX4 vg79d5 double homozygotes show a significant reduction in wing size and an uplifting of the postscutellar bristles, similar to the phenotype of vg1 homozygotes. Flies heterozygous for vg79d5 in a sdETX4 background have a phenotype almost as strong as sdETX4 vg79d5 double homozygotes. Flies heterozygous for vgnull in a sdETX4 background do not show such a significant reduction in the wing as flies heterozygous for vg79d5 in a sdETX4 or sd1 background. Females expressing vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 in a sdETX4/+ background die in the late pupal stage. They show ectopic wing-like outgrowths at the posterior of the head capsule, with strong deformation of the eye. Males expressing vgScer\UAS.cZa under the control of Scer\GAL4ptc-559.1 in a hemizygous sdETX4 background do not show such extensive wing-like outgrowths and show only slight deformation of the eye. These males can emerge from the pupae, although they die within a few hours.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\TDUScer\UAS.cVa under the control of Scer\GAL4ptc-559.1 or Scer\GAL4da.G32 results in a large increase in eye size. This is partially suppressed in a sdETX4 background.

Defects in the wing blade can be rescued by constructs carrying Hsap\TCF13hs.PD.

Complementation and Rescue Data
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

Weak sd mutation.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
References (20)