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General Information
Symbol
Dmel\sdtEH681
Species
D. melanogaster
Name
FlyBase ID
FBal0032675
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sdtEH
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

C8225124T

Reported nucleotide change:

C?T

Amino acid change:

Q156term | sdt-PB; Q231term | sdt-PE; Q884term | sdt-PG; Q133term | sdt-PI; Q372term | sdt-PJ; Q786term | sdt-PL; Q884term | sdt-PM; Q156term | sdt-PN; Q470term | sdt-PO

Reported amino acid change:

Q156term

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

Nucleotide substitution: C?T.

Amino acid replacement: Q156term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Homozygous clones in the wing disc survive and do not show polarity defects.

sdtEH681 follicle cell clones, induced by driving Scer\FLP1Scer\UAS.cUa under the control of the Scer\GAL4e22c driver, are morphologically indistinguishable from wild type.

sdtEH681 embryos show reduced cuticle production, typically resulting in embryos with fragmented cuticle.

Homozygous eye clones have no obvious effects on photoreceptor cell morphogenesis. No light-induced retinal degeneration is seen.

Epithelial cells of ectodermal origin lose their apicobasal polarity resulting in the loss of epithelial integrity and cell death. Both epidermal and amnioserosa cells of stage 10 lack zonula adherens junctions (ZA) and the number of spot adherens junctions (SAJ) is lower. The structure of all ectodermally derived epithelia is affected to varying extents.

First defects in zonula adherens formation are seen at the onset of germband extension on the dorsal side of the embryo in the developing amnioserosa. The distribution of adherens junction material at the apicolateral boundary is more irregular.

Cell death is dramatically increased. Although no extra mesoderm cells are induced to become hemocytes the macrophages become larger than average. Cellular debris becomes located extracellularly in the hemolymph.

At stage 11 of embryogenesis ectodermal cells lose their normal columnar shape and round up. 1-2hr later cells form clusters, rather than a monolayer. Dead cells are often seen on the outside of the embryo. Vesicles form of cells retaining epithelial characteristics, and later secrete grains or vesicles of cuticle. Many cells fail to become integrated into an epithelial sheet. Degeneration is most extreme in the epidermis, the pharynx and the amnioserosa, and least extreme in the proventriculus and Malpighian tubules which develop almost normally.

Aberrant organogenesis and small shreds of cuticle.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhancer of
Statement
Reference
Suppressor of
Statement
Reference
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments

Expression of sdtA.Scer\UAS under the control of the maternal driver Scer\GAL4nos.PG partially rescues the cuticle phenotype seen in sdtEH681 mutant embryos. 43% of embryos examined showed continuous cuticle over 2/3 of the surface compared to 8% in the negative control.

Expression of sdtB.Scer\UAS under the control of the maternal driver Scer\GAL4nos.PG partially rescues the cuticle phenotype seen in sdtEH681 mutant embryos. 13% of embryos examined showed continuous cuticle over 2/3 of the surface compared to 8% in the negative control and approximately 10% only displayed fragments of cuticle compared to over 70% in controls.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

sdtEH681, sdt7D, sdt2, sdt3, sdtK8 and Df(1)HA11 all have a similar phenotype. The sdt mutant phenotype is not enhanced by deleting the sdt gene from the maternal germline. The phenotype of amorphic sdt mutants is essentially the same as the phenotype of amorphic crb mutants. Embryos doubly mutant for sdt and crb mutants show the same phenotype as embryos singly mutant for either gene. Phenotype is unaffected by a duplication for crb+, Dp(3;3)M95A+13.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (3)
References (15)