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General Information
Symbol
Dmel\sevS11.Tag:MYC
Species
D. melanogaster
Name
FlyBase ID
FBal0032692
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
sevS11, sE-sevS11, sev-S11
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference

A constitutively active form of sev is expressed under the control of two copies of the sev enhancer fused to an Hsp70 promoter.

A truncated, activated sevenless kinase overexpressed under the control of a duplicated sev enhancer.

sev amino acids 1865 to 2554, lacking most of the extracellular domain, encoded by cDNA are under the control of the duplicated sev enhancer and Hsp70 promoter. N-terminal signal sequence is provided by an inframe fusion with the signal peptide of Lcp3, and the protein is tagged with a Tag:MYC epitope.

Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Flies expressing sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev have a rough eye phenotype. Extra R7 photoreceptor cells are present in the ommatidia.

The eyes of flies that express sevS11.T:Hsap\MYC show a greater number of photoreceptor cells per ommatidium than wild-type flies.

Flies carrying sevS11.T:Hsap\MYC have a rough eye phenotype, with an average of 4.3 R7 cells per ommatidium.

Flies expressing sevS11.T:Hsap\MYC have extra R7 photoreceptors in the eye and occasionally have extra outer photoreceptor cells. Cells outside the ommatidial preclusters can adopt an R4 fate in mutant eye imaginal discs.

Expression of sevS11.T:Hsap\MYC causes a rough eye phenotype and a transformation of cone cells into additional R7 cells.

Flies carrying sevS11.T:Hsap\MYC have an average of 2.5 ectopic R7 cells per ommatidium.

Mutants have abnormal eyes - ectopic R7 photoreceptors are in nearly all ommatidia.

Expression of sevS11.T:Hsap\MYC leads to the recruitment of supernumerary R7 photoreceptor cells, exhibiting a characteristic rough pattern of irregular ommatidia.

Expression of sevS11.T:Hsap\MYC causes a rough eye phenotype due to the transformation of cone cells into additional R7 cells.

Flies carrying sevS11.T:Hsap\MYC have rough eyes, due to the transformation of cone cells into supernumerary photoreceptors, mainly R7 cells.

Flies carrying sevS11.T:Hsap\MYC have an average of 3.38 +/- 0.26 R7 photoreceptor cells per ommatidium.

sevS11.T:Hsap\MYC mutant eyes do not exhibit melanotic tumours.

Mutant flies have rough eyes.

Flies carrying sevS11.T:Hsap\MYC have rough eyes with disorganised morphology, due to the presence of multiple R7 cells per ommatidium and frequent ommatidial fusions.

Flies carrying sevS11.T:Hsap\MYC have a rough eye phenotype.

Heterozygotes exhibit roughening of patches on both eyes. Homozygotes exhibit blistering in patches on one or both eyes. The major effect is the production of ectopic R7 photoreceptors up to a maximum of 4 per individual ommatidium. Mutation results in migration of ectopic photoreceptors beneath the basal membrane.

Rough eye phenotype.

Adults exhibit a rough eye phenotype that cannot be suppressed by heterozygosity for Jra (Jra76-19).

Appearance of ectopic photoreceptor cells in the adult eye is suppressed by loss of a functional copy of Jra (Jra1 or JraIA109).

Su(Raf) Hsp83 mutations and Hsp83e6D interact genetically with the sev mutations, other E(sev) Hsp83 mutations fail to interact.

Extra R7 phenotype is suppressed by mutations in lilli.

Expression in stage 11 embryos does little harm to the developing tracheal system despite strong deleterious effects on viability. Expression at stage 13 induces numerous ectopic secondary or terminal branches.

Expression of P{sE-dos} restores the multiple R7 phenotype in sevS11.T:Hsap\MYC heterozygotes.

Overexpression of P{hs-argos.S} fails to inhibit the formation of multiple R7 cells.

Supernumerary R7 cell formation. Injection of a Ras CAAX peptidomimetic, FTI-254, fails to rescue the mutant eye phenotype.

Extra R7 cells formed in ommatidia.

Rough eye phenotype is suppressed by JrabZIP.hs.sev.T:Ivir\HA1.

Flies expressing sevS11.T:Hsap\MYC have rough eyes.

Transformation of mystery cells and cone cells into R7 cells (Basler et al., Cell 64:1069--1082 ).

Rough eye, irregular ommatidial pattern. Most ommatidia have more than 6 photoreceptor cells, several of which have rhabdomeres characteristic of R7 cells. sevS11.T:Hsap\MYC; ort double mutants exhibit 4 small rhabdomeres per ommatidium. sev14, sevS11.T:Hsap\MYC flies are attracted to UV light suggesting that some of the supernumerary R7-like cells make functional synaptic connections.

Flies carrying sevS11.T:Hsap\MYC in a sev mutant background have rough eyes. Each ommatidium contains on average 6 large rhabdomeres and 4 small rhabdomeres.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Statement
Reference

sevS11.Tag:MYC has visible phenotype, suppressible | partially by mask[+]/mask5.8

sevS11.Tag:MYC has visible phenotype, suppressible | partially by mask[+]/mask10.22

sevS11.Tag:MYC has visible phenotype, suppressible | partially by Egfrf2

NOT suppressed by
Statement
Reference

sevS11.Tag:MYC has visible phenotype, non-suppressible by cdi33-077

sevS11.Tag:MYC has visible phenotype, non-suppressible by cdi34-165

sevS11.Tag:MYC has visible phenotype, non-suppressible by cdi44-004

sevS11.Tag:MYC has visible phenotype, non-suppressible by ShcBG

sevS11.Tag:MYC has visible phenotype, non-suppressible by Shc111-40

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by ash12

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by Vha5515

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by Vha5516

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by mor2

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by osa1

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by sls1

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by skd1

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by vtd3

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by Pc3

Ras85DV12.sev, sevS11.Tag:MYC has lethal phenotype, non-suppressible by Pc4

Other
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Statement
Reference

sevS11.Tag:MYC has phenotype, non-enhanceable by lillis35

sevS11.Tag:MYC has phenotype, non-enhanceable by lilliXS407/lilliXS407

sevS11.Tag:MYC has phenotype, non-enhanceable by lilliXS575

Suppressed by
Statement
Reference

sevS11.Tag:MYC has cone cell phenotype, suppressible by dosR31

sevS11.Tag:MYC has eye phenotype, suppressible by dosR31

sevS11.Tag:MYC has ommatidium phenotype, suppressible by kay2

sevS11.Tag:MYC has eye phenotype, suppressible | partially by Sps1k11320

sevS11.Tag:MYC has eye phenotype, suppressible | partially by Catn1

sevS11.Tag:MYC has ommatidium phenotype, suppressible | partially by Catn1

sevS11.Tag:MYC has eye phenotype, suppressible | partially by Egfrf2

sevS11.Tag:MYC has phenotype, suppressible by rin1/rin[+]

sevS11.Tag:MYC has ommatidium phenotype, suppressible by rin1/rin[+]

sevS11.Tag:MYC has photoreceptor cell R7 | ectopic phenotype, suppressible by rin1/rin[+]

sevS11.Tag:MYC has phenotype, suppressible by rin2/rin[+]

sevS11.Tag:MYC has ommatidium phenotype, suppressible by rin2/rin[+]

sevS11.Tag:MYC has photoreceptor cell R7 | ectopic phenotype, suppressible by rin2/rin[+]

sevS11.Tag:MYC has eye phenotype, suppressible by dosY854F.hs.2sev

sevS11.Tag:MYC has eye phenotype, suppressible by Nrtunspecified

sevS11.Tag:MYC has eye phenotype, suppressible by DabS119A.hs.2sev

sevS11.Tag:MYC has eye phenotype, suppressible by cnkXE-1040

sevS11.Tag:MYC has eye photoreceptor cell phenotype, suppressible by Jra1/Jra[+]

sevS11.Tag:MYC has eye photoreceptor cell phenotype, suppressible by JraIA109/Jra[+]

sevS11.Tag:MYC has phenotype, suppressible by dos1.46

sevS11.Tag:MYC has phenotype, suppressible by dos2.42

sevS11.Tag:MYC has phenotype, suppressible by dosunspecified

sevS11.Tag:MYC has phenotype, suppressible by dosR31

sevS11.Tag:MYC has phenotype, suppressible by dosP115

sevS11.Tag:MYC has eye phenotype, suppressible by TfIIA-SE73

NOT suppressed by
Statement
Reference

sevS11.Tag:MYC has eye phenotype, non-suppressible by cdi33-077

sevS11.Tag:MYC has eye phenotype, non-suppressible by cdi34-165

sevS11.Tag:MYC has eye phenotype, non-suppressible by cdi44-004

sevS11.Tag:MYC has ommatidium phenotype, non-suppressible by kayhs.PZ/kay2

sevS11.Tag:MYC has phenotype, non-suppressible by lilliXS407/lilliXS407

sevS11.Tag:MYC has phenotype, non-suppressible by lilliXS575

sevS11.Tag:MYC has phenotype, non-suppressible by lillis35

sevS11.Tag:MYC has eye phenotype, non-suppressible by ShcBG

sevS11.Tag:MYC has ommatidium phenotype, non-suppressible by ShcBG

sevS11.Tag:MYC has eye phenotype, non-suppressible by Shc111-40

sevS11.Tag:MYC has ommatidium phenotype, non-suppressible by Shc111-40

Enhancer of
Statement
Reference

sevS11.Tag:MYC is an enhancer of phenotype of svp1.sev

sevS11.Tag:MYC is an enhancer of phenotype of svp2.sev

Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of drkEP2477 under the control of Scer\GAL4hs.2sev in a sevS11.T:Hsap\MYC genetic background results in a strong rough eye phenotype.

Expression of Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev in a sevS11.T:Hsap\MYC genetic background rescues the rough-eye phenotype.

Expression of drkEP2477 and Socs36EScer\UAS.P\T.cCa under the control of Scer\GAL4hs.2sev in a sevS11.T:Hsap\MYC genetic background results in an intermediate rough-eye phenotype.

Co-expression of either Socs36E34-120y+ or Socs36E34-120y- suppresses the rough eye phenotype caused by expression of sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev.

Co-expression of Socs36EScer\UAS.P\T.cCa suppresses the rough eye and extra R7 photoreceptor cell phenotype caused by expression of sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev.

Expression of drkEP2477 under the control of Scer\GAL4hs.2sev enhances the rough eye and extra R7 photoreceptor cell phenotype caused by sevS11.T:Hsap\MYC.

Co-expression of Socs36ESH2.Scer\UAS.P\T does not suppress the rough eye or extra R7 photoreceptor cell phenotype caused by expression of sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev.

Co-expression of Socs36EΔSB.Scer\UAS.P\T suppresses the rough eye and extra R7 photoreceptor cell phenotype caused by expression of sevS11.T:Hsap\MYC under the control of Scer\GAL4hs.2sev.

The increased photoreceptor cell phenotype seen in sevS11.T:Hsap\MYC flies is suppressed when flies coexpress either S6kIIScer\UAS.T:Hsap\MYC or S6kIIK231R.Scer\UAS.T:Hsap\MYC under the control of Scer\GAL4hs.2sev.

Expression of either cdi33-077, cdi34-165 or cdi44-004 under the control of Scer\GAL4hs.2sev suppresses the rough eye phenotype of sevS11.T:Hsap\MYC flies. In the absence of the Scer\GAL4hs.2sev driver, no suppression is seen.

Expression of either cdi34-165y- or cdi44-004y- under the control of Scer\GAL4hs.2sev suppresses the rough eye phenotype of sevS11.T:Hsap\MYC flies.

Expression of cdi33-077y- under the control of Scer\GAL4hs.2sev does not suppress the rough eye phenotype of sevS11.T:Hsap\MYC flies.

Expression of cdiScer\UAS.cSa under the control of Scer\GAL4hs.2sev partially suppresses the extra R7 photoreceptor cell phenotype of sevS11.T:Hsap\MYC flies.

Expression of mRpL55Scer\UAS.cSa under the control of Scer\GAL4hs.2sev does not suppress the eye phenotype of sevS11.T:Hsap\MYC flies.

Expression of sshdsRNA.Scer\UAS.T:Avic\GFP under the control of Scer\GAL4hs.2sev partially suppresses the extra R7 photoreceptor cell phenotype of sevS11.T:Hsap\MYC flies.

tsr2/tsrntf weakly suppresses the extra R7 photoreceptor cell phenotype of sevS11.T:Hsap\MYC flies.

Co-expression of Mkp3hs.sev almost completely suppresses the extra R7 photoreceptor cell phenotype seen in flies expressing sevS11.T:Hsap\MYC.

The addition of dosR31 almost completely suppresses the rough eye phenotype seen in sevS11.T:Hsap\MYC animals. The addition of both dosR31 and doshs.2sev does not suppress the phenotype. The addition of both dosR31 and dosR644K.R696K.hs.2sev or dosR696K.hs.2sev continues to suppress the rough eye phenotype. The addition of both dosR644K.hs.2sev partially suppresses the rough eye phenotype.

mask10.22 dominantly suppresses the ectopic R7 phenotype caused by sevS11.T:Hsap\MYC; the double mutant flies have an average of 1.8 ectopic R7 cells per ommatidium. mask5.8 dominantly suppresses the ectopic R7 phenotype caused by sevS11.T:Hsap\MYC; the double mutant flies have an average of 1.9 ectopic R7 cells per ommatidium.

When SelDk11320 is added to sevS11.T:Hsap\MYC flies, there is an increase of wild-type ommatidia from less than 10% to more than 50%. Only 7.5% of ommatidia in sevS11.T:Hsap\MYC eyes contain one R7 cell, the addition of SelDk11320 increases that to 61.2%. The addition of Catn1 also suppresses these phenotypes.

The addition of heterozygous dosR31 or dosP115 largely suppresses the sevS11.T:Hsap\MYC rough eye phenotype. The addition of doshs.T:Avic\GFP to dosR31/sevS11.T:Hsap\MYC flies restores the multiple R7 phenotype.

The extra R7 photoreceptor cells seen in flies carrying sevS11.T:Hsap\MYC are reduced to an average of 2.71 +/- 0.23 or 2.32 +/- 0.11 R7 photoreceptor cells per ommatidium if the flies are also carrying rin1 or rin2 respectively.

Expression of CblDv.Scer\UAS in the eye under the control of Scer\GAL4GMR.PF in combination with sevS11.T:Hsap\MYC at 29[o]C produces melanotic tumours in the eyes in approximately 40% of progeny.

vapScer\UAS.T:Hsap\MYC does not suppress the sevS11.T:Hsap\MYC phenotype when expressed under the control of Scer\GAL4hs.2sev.

The sevS11.T:Hsap\MYC rough eye phenotype is dominantly suppressed by Nrtunspecified or DabS119A.hs.2sev and is enhanced by DabΔPro.hs.2sev.

Ras85DV12.sev in combination with sevS11.T:Hsap\MYC results in synthetic lethality. Expression of sevS11.T:Hsap\MYC above a certain level in embryos results in lethality and this lethality is enhanced by co-expression of Ras85DV12.sev. The lethality is sensitive to the expression levels of both sevS11.T:Hsap\MYC and Ras85DV12.sev. Less than 10% of sevS11.T:Hsap\MYC Ras85DV12.sev embryos form cuticle. Those embryos that do form cuticle generally have missing or malformed anterior structures including head parts and thoracic segments, as marked by absence of denticle belts. Approximately one-third of the embryos show curling of the cuticle, possibly due to a failure in germband retraction, and approximately one-tenth of embryos have a weak dorsal closure phenotype. A small fraction of embryos have missing or fused abdominal denticle belts, swollen anterior segments or completely lack identifiable structures and form a ball of cuticle. sevS11.T:Hsap\MYC embryos show a similar range of phenotypes. sevS11.T:Hsap\MYC is not lethal in combination with phylsev.PC, rosev.PI, aopACT.sev, phylhs.sev or Ras85DN17.hs.2sev. The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by Trl62, trxunspecified, Df(2L)JK12, skd2, osa2, mor1, kto1, kis1, kis2, brm1, brm2, Df(3R)red-P52, RpII140AS, RpII15V, SR3-8XS-701, SR3-8S-959, SR3-7XS-17N, SR3-7S-499, Taf1XS-2232, Taf1S-625, Su(Tpl)S-705, Su(Tpl)S-192, Taf4S-466, Taf4XS-793, Taf6XS-922, Sose4G, Ras85De1B, Ras85DE4, rlXS-2275, rlXS-2442, ksrXS-2424, ksrXS-2832, phyl2829, phyl2245, βggt-IS-2554, mamS-176, Df(3R)293γ5, Df(2L)ast2, Df(2R)nap9, Df(2R)pk78c or Df(3L)vin2. The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by l(3)0624006240, l(3)0626506265, l(3)0929109291, Rpd304556, l(3)0514305143, btl00208, btl04727, frc10098, ftz-f103649, eRF105637, l(3)77CDf04521, tws02414, tws06848, l(3)0360803608, l(3)0665806658, osa04539, fray07551, l(3)0582005820, InR05545, pnt04263b, pnt07825, l(3)0307703077, l(3)0470804708, l(3)0674306743 or l(3)0673406734. The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is suppressed by the l(3)06948 line (l(3)06948a06948a and sr06948b), by the l(3)03745 line (tws03745a and Dl03745b) and by the l(3)04704 line (l(3)04704a04704a and l(3)04704b04704b). The lethality caused by sevS11.T:Hsap\MYC in combination with Ras85DV12.sev is not suppressed by TfIIA-SE32, ash12, Vha5515, Vha5516, mor2, osa1, sls1, skd1, Df(3R)urd, urd2, vtd3, Pc3 or Pc4.

Enhancer of the dosage-dependent (two or more copies of P{sev-svp1} or P{sev-svp2}) transformation of cone cells into R7 photoreceptors and at a lower frequency R7 cells into outer photoreceptors.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1)
Notes on Origin
Discoverer
Comments
Comments

The number of ectopic R7 cells is highly sensitive to the wild-type genetic background.

Constitutively activated sev kinase.

Ligand independent allele.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (9)
References (58)