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General Information
Symbol
Dmel\sggM11
Species
D. melanogaster
Name
FlyBase ID
FBal0032709
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
zw3M11-1, zw3m11, sggM11-1
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Maternally and zygotically mutant sggM11 embryos (coming from mothers whose whole germline constitutes from sggM11 mutant cells only - created by the OvoD germline clone technique) show a strong 'naked cuticle' phenotype.

sggM11 mutants exhibit a complete loss of embryonic patterning due to hyperactivation of the canonical Wnt pathway, and a completely naked cuticle.

Homozygous clones in the wing result in ectopic bristles on the wing blade and margin.

Embryos mutant for sggM11 both maternally and zygotically display naked embryonic cuticle. No cells secrete denticles.

Homozygous sggM11 germ-line clone-derived embryos display a naked ventral cuticle phenotype.

sggM11 mutant embryos derived from maternal homozygous mutant germ line clones exhibit distinct perturbation of segmentation that is revealed in a loss of all denticles. These embryos do not survive.

sggM11 mutant wing blade clones exhibit thin mechanosensory bristles. Ectopic bristles can be generated anywhere on the wing blade by sggM11 mutant clones.

No denticle are present in the cuticles made by embryos maternally and zygotically mutant for sggM11.

Homozygous clones in the wing show transformations of wing blade to wing margin tissue.

Mutant stage 17 embryos do not show significant defects in the location or formation of the tracheal dorsal trunk branch, dorsal branch 10, spiracular branch 10 or the posterior spiracle.

Homozygous sggM11 or sgg32/sggM11 adults (that have been rescued to adulthood by the addition of sgghs.P coupled with larval and pupal heatshocks), show a lengthening of the locomotor activity rhythm periodicity to about 26 hours.

Extra Malpighian tubule cells are specified in sggM11 embryos dervied from sggM11 germ-line clones.

slou-expressing muscles are often absent in embryos derived from homozygous germline clones.

In embryos derived from germ line clones, the anterior midgut construction is obliterated. Phenotype is similar to that caused by Apc2 RNAi mutants.

Mutant clones generated via Scer\GAL4en-e16E and Scer\FLP1Scer\UAS.cDa acting on P{FRT(whs)}101 show ectopic bristles limited to the posterior compartment.

Embryos do not form ventral denticles.

Embryos zygotically and maternally mutant for sggM11 show reduction in the number of heart precursors. sggM11 dsh75 double mutant embryos have the same phenotype as sggM11 single mutant embryos.

Females with homozygous germ line clones, mated to wild-type males, give rise to 2 classes of embryos: null sggM11/Y embryos (lacking both maternal and zygotic sgg function) which have a completely naked cuticle and paternally rescued sggM11/+ embryos (lacking maternal sgg function) which have sparse denticles. These phenotypes are rescued to an almost wild-type denticle pattern by sggScer\UAS.cSa expressed under the control of Scer\GAL469B.

Mutant embryos show abnormal guts.

Homozygous clones induced in the eye produce scars. Outside the scarred area, ommatidial chirality is inverted on the polar side of the clone. This phenotype is unchanged if the clone is also homozygous for wgl-17.

No effect on the faf eye phenotype.

Embryos show severe segmentation defects but no increase (but rather a moderate decrease) in heart precursor formation.

In paternally rescued embryos the gut constrictions are highly abnormal, all three are condensed and folded up into one giant constriction. Malpighian tubule growth is precociously initiated. Homozygous embryos also exhibit precocious development, but are severely misshapen so morphological analysis is difficult.

More than wild type numbers of invagination folds in the stomodeal invagination are observed.

Virtually all ventral denticle belts of the embryo are replaced with naked cuticle.

Flies lacking both maternal and zygotic sgg have completely naked cuticles, regardless of the dose of ci.

Clones induced during the late second larval instar give rise to fully ventralized dorsal bristles in both anterior and posterior compartments.

Clones double mutant for sggM11 and Df(3R)Espl22 in the anterior wing differentiate anterior bristles (as for clones of sggM11 single mutants), however double mutant clones in the posterior compartment differentiate hairs typical of the posterior wing margin.

Clones of sgg in the wing blade autonomously differentiate tufts of bristles characteristic of the wing margin. Clones of cells simultaneously mutant for both dsh and sgg do not produce a dsh mutant phenotype at the wing margin, but display the phenotype of cells mutant for sgg alone.

Embryos lacking both maternal and zygotic sgg and arm function have an embryonic cuticle phenotype like that of arm mutant embryos alone. Embryos lacking maternal sgg and arm but receiving wild type paternal copies develop into normal embryos that survive until adulthood. Embryos lacking maternal sgg and arm but receiving a wild type paternal copy of arm+ only, show a sgg naked cuticle phenotype.

Embryos derived from homozygous mutant germ lines, receiving neither maternal or zygotic wild type product, lack denticles on the ventral cuticle.

Homozygous clones in the wing generally contain dense aggregations of bristles, the type of bristle depending on the location of the clone. The spacing of the normal campaniform sensilla is disrupted if the clone is near the normal location of the sensilla. Ectopic veins are sometimes formed.

Mutants embryos lacking both maternal and zygotic gene product completely lack ventral denticles: en expression is expanded and there is a strong ectopic wg stripe of expression. Embryos lacking only maternal component have some ventral denticles: en expression is identical to that in the "null" embryos but the ectopic wg stripes are sparse.

Hemizygous progeny derived from heterozygous females die during early larval stages. All embryos derived from homozygous germline clones die during embryonic development, and have very poorly differentiated cuticle which sometimes has holes in it. The head is defective, with abnormal spiracles, and the filzkorper material is very prominent. The CNS is very disorganised and the brain often protrudes dorsally rather than being covered by epidermis. The PNS is abnormal. Some embryos have a less extreme phenotype, with more cuticle differentiation and some defective denticle belt differentiation. The phenotype can be partially rescued by a paternal copy of wild-type sgg. Homozygous clones in the wing show homeotic transformation of hairs into bristles.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Suppressed by
Enhancer of
NOT Enhancer of
Statement
Reference

sggM11 is a non-enhancer of visible phenotype of Scer\GAL4bbg-C96, mamN.UAS

Suppressor of
NOT Suppressor of
Statement
Reference
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
NOT Enhancer of
Suppressor of
Statement
Reference

sggM11/sgg[+] is a suppressor of scutum & macrochaeta phenotype of Scer\GAL4ap-md544, nmoc5-1.UAS

sggM11/sgg[+] is a suppressor | partially of wing phenotype of Scer\GAL4ap-md544, nmoc5-1.UAS

sggM11 is a suppressor of wing disc | ectopic phenotype of Pka-C1E95, smo3

sggM11 is a suppressor | somatic clone of proneural cluster & dorsal mesothoracic disc phenotype of NECNΔ10-12.UAS, Scer\GAL4ptc-559.1

sggM11/sgg[+] is a suppressor of wing vein | ectopic phenotype of Scer\GAL4en-e16E, armUAS.cWa

NOT Suppressor of
Other
Additional Comments
Genetic Interactions
Statement
Reference

armF1α; sggM11 double mutants exhibit normal denticle development. Expression of aPKCΔN.Scer\UAS under the control of Scer\GAL4da.G32 in these double mutants display randomly oriented denticles. Cells secreting denticles are not rectangular or aligned in regular rows. This effect is specific to sggM11 mutants as neither expression of aPKCΔN.Scer\UAS in otherwise wild-type embryos, nor in armF1α mutant embryos show an effect on denticle organisation.

A sggM11 mutant background fails to affect the epithelial patterning defects found in armF1α mutants.

armF1α; sggM11; aPKCk06403 triple mutants exhibit significant denticle and cell disruption. Specifically, proper cell shapes and cell alignment are lost, and instead of organization into even rows, cells curved and formed denticle swirls.

Cuticles of embryos double mutant maternally and zygotically for both sggM11 and arm2 resemble that of arm2 single mutants.

Embryos triple mutant for sggM11 both maternally and zygotically and arm2 both maternally and zygotically and l(2)gl4 zygotically show a cuticle phenotype that is similar to that of zygotic l(2)gl4 single mutants.

Cuticles of embryos double mutant maternally and zygotically for armF1α and sggM11 resemble that of armF1α single mutant embryos.

Embryos triple mutant for sggM11 both maternally and zygotically and armF1α both maternally and zygotically and l(2)gl4 zygotically show a cuticle phenotype that is similar to that of zygotic l(2)gl4 single mutants.

Cuticles of embryos double mutant for sggM11 both maternally and zygotically and l(2)gl4 zygotically resemble that of l(2)gl4 single mutants.

Expression of AxnScer\UAS.cHa under the control of Scer\GAL4da.G32 suppresses the ventral denticle belt phenotype of sggM11 mutant embryos.

Expression of AxnScer\UAS.T:Src64B,T:Zzzz\FLAG under the control of Scer\GAL4da.G32 weakly suppresses the ventral denticle belt phenotype of sggM11 mutant embryos.

Expression of Apc2Scer\UAS.T:Avic\GFP has no effect on the ventral denticle belt phenotype of sggM11 mutant embryos.

Expression of Apc2Scer\UAS.T:Src64B,T:Zzzz\FLAG has no effect on the ventral denticle belt phenotype of sggM11 mutant embryos.

Zygotic expression of gsktScer\UAS.cKa driven by Scer\GAL4arm.PS in maternal mutant sggM11 embryos partially restores segmentation.

Unlike embryos from dsh3 germline clones, embryos from dsh3; sggM11 germ-line clones have no dorsal hole in their cuticles (although their dorsal cuticle is severely puckered), and have a dorso-ventrally polarised and extended leading edge at stage 13.

The addition of sggM11 has no effect on the arm2 embryonic phenotype. However the combination of arm2, armS8 and sggM11 leads to embryos with naked cuticle.

Notching of the distal wing margin in nmoc5-1.Scer\UAS; Scer\GAL4sd-SG29.1 flies is completely suppressed by sggM11/+. Loss of notum macrochaetae in nmoc5-1.Scer\UAS; Scer\GAL4ap-md544 flies is suppressed by sggM11/+ and enhanced by AxnA2.Scer\UAS, leading to the loss of all scutellar bristles. Severe blistering of the wing in nmoc5-1.Scer\UAS; Scer\GAL4ap-md544 flies is suppressed by sggM11/+, resulting in almost wild-type wings.

The loss of denticle belts in the cuticle made by sggM11/sggM11 maternal/zygotic mutant embryos, is suppressed by maternal/zygotic armF1α/armF1α (a weaker suppression is seen when arm8 is used instead). When these double mutant embryos are also zygotically mutant for wgl-8, they make little naked cuticle, a phenotype approximating to that seen in wgl-8 mutants. The appearance of the denticle lawn in these embryos is noticeably different to that seen in maternal/zygotic armF1α/armF1α embryos zygotically mutant for wgl-8. The denticle belts formed in sggM11; armF1α maternal/zygotic double mutant embryos are largely suppressed by AxnS044230/AxnS044230, leaving mainly naked cuticle without much sign of periodic character. The periodic character of the cuticles made by sggM11; arm8 maternal/zygotic double mutant embryos is suppressed by arr2/arr2, with the resulting cuticle tending toward denticle cell fates.

lgs20F, sggM11 homozygous mutant germ-line clones can made by rescuing homozygous mothers with P{αTub84B(FRT.lgs.sgg)Scer\GAL4.B} and FLPing out sggmR10 and lgsαTub84B.PK (Which are surrounded by FRT sites). When this is done, the phenotype of the animals is indistinguishable from lgs20F germ-line clones.

Pka-C1E95, sggM11 smo3 triple mutant clones in the wing disc exhibit some outgrowths from the notum but no significant wing pattern duplications.

sggM11 somatic clones, in a NECNΔ10-12.Scer\UAS/Scer\GAL4ptc-559.1 background, suppress the loss of proneural precursors and sensory organ phenotypes normally seen in these flies.

Has no effect on the wing nicking phenotype seen in mamN.Scer\UAS, Scer\GAL4C96 flies.

The sggM11 lack of denticles phenotype is not affected by injection of both dsRNA produced by annealing fz374-2360 and fza.374-2360 RNA and dsRNA produced by annealing fz2L.cKa and fz2a.L.cKa RNA.

Double mutants for sggM11 and nej3 show a worse gut phenotype than do nej3 single mutants.

Suppresses wing margin phenotype of fzScer\UAS.N, Scer\GAL469B.

Embryos doubly mutant for sggM11 and dsh3 or porPB16 also lack denticles on the ventral cuticle. Embryos doubly mutant for sggM11 and arm8 display the ventral cuticle covered with denticles.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Rescued by
Comments

The naked cuticle phenotype characteristic for both maternally and zygotically sggM11 mutant embryos cannot be rescued by Scer\GAL4arm.PU-driven expression of any of the following: sggKK-MI.Scer\UAS.T:Ivir\HA1, sggScer\UAS.T:Myr-Src64B,T:Ivir\HA1, sggKK-MI.Scer\UAS.T:Myr-Src64B,T:Ivir\HA1 but is partially rescued by co-expression of sggScer\UAS.T:Ivir\HA1.

Zygotic expression of sggScer\UAS.cSa driven by Scer\GAL4arm.PS in maternal mutant sggM11 embryos partially restores segmentation.

Zygotic expression of gsktScer\UAS.cKa driven by Scer\GAL4arm.PS in maternal mutant sggM11 embryos partially restores segmentation. The extent of sggM11 mutant rescue by gsktScer\UAS.cKa is less than the rescue by sggScer\UAS.cSa.

sggmR10 rescues hemizygous males to viable and (poorly) fertile adults.

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Synonyms and Secondary IDs (11)
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    References (67)