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General Information
Symbol
Dmel\sti3
Species
D. melanogaster
Name
FlyBase ID
FBal0032821
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
point mutation
Nucleotide change:

A12513243T

Amino acid change:

K348term | sti-PA; K352term | sti-PB

Reported amino acid change:

K348term

Comment:

Site of nucleotide substitution in mutant inferred by FlyBase based on reported amino acid change.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

The mutation results in a truncated protein only encoding part of the kinase domain.

Amino acid replacement: K348term.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

The fraction of stiZ3-5829/sti3 individuals recovered in a cross between stiZ3-5829/TM6B and sti3/TM6B animals is significantly less than expected at 25[o]C, whereas at 18[o]C, there is no significant difference between the fraction of heterozygous and stiZ3-5829/sti3 progeny recovered. At 18[o]C, both male and female stiZ3-5829/sti3 progeny are equally represented, whereas at 25[o]C, male stiZ3-5829/sti3 progeny are significantly reduced.

The nuclear diameter of stage 12-13 stiZ3-5829/sti3 follicle cells is normal at 18[o], but is increased compared to wild type at 21[o]C.

In stiZ3-5829/sti3 flies, 100% have missing eye bristles and 37% have rough eyes when reared at 21[o]C. At 25[o]C, 100% of stiZ3-5829/sti3 adults have missing eye bristles and rough eyes.

The rough eye phenotype of stidsRNA.Sym.Scer\UAS expression under the regulation of Scer\GAL4ey.PB is enhanced by the presence of sti3. sti1/sti3, sti2/sti3, and sti4/sti3 flies have very small imaginal discs and are lethal at the prepupal stage.

In trans to a deficiency, mitotic chromosomes (both sisters and heterologues) stuck together and show metaphase delay; "successful" anaphase fragments the chromosomes.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Suppressor of
Statement
Reference
Phenotype Manifest In
NOT Enhancer of
Statement
Reference

sti3 is a non-enhancer of eye & ommatidium phenotype of tumdsRNA.UAS.cBa, Scer\GAL4ey.PB

Suppressor of
Statement
Reference

sti3/sti[+] is a suppressor of eye phenotype of AGO1UAS.cZa, Scer\GAL4GMR.PF/Scer\GAL4GMR.PF

Additional Comments
Genetic Interactions
Statement
Reference

The rough and small eye phenotypes of flies expressing AGO1Scer\UAS.cZa under the control of Scer\GAL4GMR.PF are suppressed by sti3/+, with suppression consistently being stronger in females than in males.

The sti3 mutation does not modify the RacGAP50CdsRNA.Scer\UAS (under the regulation of Scer\GAL4ey.PB) rough eye phenotype.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Fails to complement
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Carpenter.

Comments
Comments

Based on lethal phase analysis the following sti alleles can be ranked from the most severe to the weakest as follows: sti3 > sti1 = sti2 = sti4 > sti5.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Name Synonyms
Secondary FlyBase IDs
    References (4)