The average number of crystal cells per embryo is reduced in homozygous stage 13-14 embryos compared to wild type.
Growth cone advance and axonal pathfinding appear normal in homozygous embryos, and there is no delay in axonal outgrowth. The longitudinal tracts and commissures appear morphologically normal. The morphology of the neuromuscular junctions appears normal in stage 17 embryos.
Embryos show severe reduction in coordinated muscle contraction when compared with wild type, and an increase in uncoordinated twitching.