FlyBase Allele Report: Dmel\tin[EC40]

The myofibrils of the dorsal vessel are arranged almost exclusively in an anterior-posterior orientation in tin^ABD ; tin³⁴⁶/tin^EC40 larvae, in contrast to wild-type larvae where they are arranged spirally. In the posterior heart region of the dorsal vessel the pattern of myofibrils is highly irregular in the mutant larvae, forming abnormal cross-shaped or 'knotted' patterns. The aorta appears thinner than normal in the mutant larvae, whereas the heart frequently has a wider diameter than normal.

The heart tube is much thinner than normal in tin^ABD ; tin³⁴⁶/tin^EC40 adults. The mutant myofibrils are arranged longitudinally and transverse spirally arranged myofibrils are almost completely absent.

tin^ABD ; tin^ABD tin³⁴⁶/tin^EC40 adults show a dramatic increase in heart failure rate after pacing of the heart by external electrical stimulus compared to the heart failure rate of control adults. The recovery rate after heart failure is dramatically decreased in the mutant adults.

tin^ABD ; tin^ABD tin³⁴⁶/tin^EC40 adults have a reduced lifespan compared to controls.

(Zaffran et al., 2006)

In tin^EC40 mutant embryos, the lymph gland and heart fail to form.

(Han and Olson, 2005)

Mutant embryos have defects in the forming mesoderm around the foregut, but do form foregut ectoderm. 65% of mutant embryos have excess cells in the dorsal midline of the b1 region of the embryonic brain; the area occupied by neuronal nuclei is increased compared to wild type. The preoral brain commissure is abnormally thin. At late embryonic stage 13, mutant embryos show a reduction in the number of apoptotic cells at the dorsal midline of the brain compared to wild type. The number of brain ganglionic mother cells is unaffected in stage 11 mutant embryos.

(Page, 2002)

Mutant embryos have mild defects in the development of the tracheal dorsal trunk.

(Franch-Marro and Casanova, 2000)

The ordered files of cells seen in wild-type midgut visceral muscle primordia are not seen in these mutant embryos. The meso18E staining cells appear to invaginate, however, movement towards the interior is ragged and no ordered file of cells form.

(Taylor, 2000)

Visceral mesoderm and cardiac progenitors do not form in mutant embryos.

(Park et al., 1998)

Heart and visceral mesoderm and a subset of the somatic muscle founders fail to form in tin mutant embryos.

(Ranganayakulu et al., 1998)

Some homozygous embryos hatch and survive as first instar larvae, even though they have midgut and body wall muscle defects. They fail to grow and show lethargic behavior and large midguts. The transverse nerve is missing, as are the dorsal neurohemal organs. Both the dorsal and ventral lateral bipolar dendrite neuron are disrupted when the transverse nerve is not formed. Segmental nerve b target muscles are abnormally innervated, and this is often associated with the loss of some muscle fibres. Transverse nerve exit glial cell cannot be found.

(Gorczyca et al., 1994)

Only small islands of visceral mesoderm remain. Those principle midgut epithelial cells that are in contact with the islands of visceral mesoderm assume an epithelial phenotype.

(Tepass and Hartenstein, 1994)

Lack visceral muscles of the midgut though not the hindgut. The midgut constriction fail to form. The heart fails to develop: cardioblasts are not formed. Somatic muscle pattern is moderately affected, with the most extreme defect in the dorsal-most muscles.

(Bodmer, 1993)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Phenotype Manifest In

Enhanced by

Statement

Reference

tin^EC40 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by zfh1^75.26

(Franch-Marro and Casanova, 2000)

tin^EC40 has embryonic/larval tracheal dorsal trunk phenotype, enhanceable by zfh1^65.34

(Franch-Marro and Casanova, 2000)

NOT Enhanced by

Statement

Reference

tin^ABD, tin^EC40 has embryonic cardioblast phenotype, non-enhanceable by edl^k06602

(Schwarz et al., 2018)

tin^ABD, tin^EC40 has heart primordium phenotype, non-enhanceable by edl^k06602

(Schwarz et al., 2018)

Suppressed by

Statement

Reference

tin^EC40 has larval pericardial cell phenotype, suppressible by tin::Mmus\Nkx2-5^{2.5HD-NK2SD.hs}

(Park et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/Mmus\Nkx2-5^UAS.cRa

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/Mmus\Nkx2-5^UAS.cRa

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by tin::Mmus\Nkx2-5^{UAS.1-53:54-319}/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by tin::Mmus\Nkx2-5^UAS.Nkx-tin/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^UAS.HDs

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^UAS.HDs

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/tin::Mmus\Nkx2-5^UAS.tin-Nkx/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/tin::Mmus\Nkx2-5^UAS.tin-Nkx/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^{UAS.tin-HD,Nkx}

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^{UAS.tin-HD,Nkx}

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^{UAS.tin.1-220}

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/tin::Mmus\Nkx2-5^{UAS.tin.1-220}

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by tin::Mmus\Nkx2-5^{UAS.tin.1-134}/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by tin::Mmus\Nkx2-5^{UAS.tin.1-134}/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has visceral trunk mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Mmus\Nkx2-5^UAS.ΔNK/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has embryonic/larval heart & mesoderm phenotype, suppressible by Scer\GAL4^twi.PB/Mmus\Nkx2-5^UAS.ΔNK/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

NOT suppressed by

Statement

Reference

tin^ABD, tin^EC40 has embryonic cardioblast phenotype, non-suppressible by edl^k06602

(Schwarz et al., 2018)

tin^ABD, tin^EC40 has heart primordium phenotype, non-suppressible by edl^k06602

(Schwarz et al., 2018)

tin^EC40 has phenotype, non-suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/bap^UAS.cRa

(Ranganayakulu et al., 1998)

tin^EC40 has phenotype, non-suppressible by Scer\GAL4^twi.PB/Mmus\Nkx2-5^UAS.ΔC/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 has phenotype, non-suppressible by Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR/Rnor\Nkx2-1^UAS.cRa

(Ranganayakulu et al., 1998)

Enhancer of

Statement

Reference

tin^EC40 is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of zfh1^75.26

(Franch-Marro and Casanova, 2000)

tin^EC40 is an enhancer of embryonic/larval tracheal dorsal trunk phenotype of zfh1^65.34

(Franch-Marro and Casanova, 2000)

Suppressor of

Statement

Reference

tin^EC40 is a suppressor | partially of visceral trunk mesoderm phenotype of Mmus\Nkx2-5^UAS.cRa, Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

(Ranganayakulu et al., 1998)

tin^EC40 is a suppressor | partially of embryonic/larval heart & mesoderm phenotype of Mmus\Nkx2-5^UAS.cRa, Scer\GAL4^Mef2.PR, Scer\GAL4^twi.PB

(Ranganayakulu et al., 1998)

Other

Statement

Reference

Cdc42[+]/Cdc42³, tin^EC40 has adult heart phenotype

(Qian et al., 2011)

Additional Comments

Genetic Interactions

Statement

Reference

The cardioblast patterning defects observed in tin^EC40/tin^ABD mutant embryos are not modified by edl^k06602.

(Schwarz et al., 2018)

tin^EC40/+; Cdc42³/+ double heterozygotes exhibit a significantly increased pacing-induced heart failure rate.

(Qian et al., 2011)

The addition of zfh1^75.26 or zfh1^65.34 to tin^EC40 mutants leads to a tracheal dorsal trunk that is almost completely absent.

(Franch-Marro and Casanova, 2000)

Xenogenetic Interactions

Statement

Reference

The formation of pericardial cells is restored when tin::Mmus\Nkx2-5^{2.5HD-NK2SD.hs} is expressed in tin^EC40 embryos at 3.5 to 4.5 hours of development, although the cells are in a disorganised pattern.

(Park et al., 1998)

Effect on visceral mesoderm can be rescued by Mmus\Nkx2-5^Scer\UAS.cRa expression but not Rnor\TTF-1^Scer\UAS.cRa nor bap^Scer\UAS.cRa. tin::Mmus\Nkx2-5^{Scer\UAS.1-53:54-319} and tin^Scer\UAS.ΔC fully rescue visceral mesoderm defect but only partially rescue cardiac mesoderm defects of tin^EC40. Mmus\Nkx2-5^Scer\UAS.cRa and tin::Mmus\Nkx2-5^{Scer\UAS.Nkx-tin} partially suppress the visceral mesoderm defect but do not suppress the cardiac mesoderm defects of tin^EC40. Mmus\Nkx2-5^{Scer\UAS.ΔNK} partially suppresses both the visceral and the cardiac mesoderm defects of tin^EC40.

(Ranganayakulu et al., 1998)

Complementation and Rescue Data

Rescued by

tin^EC40 is rescued by tin^UAS.cRa/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

Partially rescued by

tin^EC40 is partially rescued by tin^UAS.ΔC/Scer\GAL4^twi.PB/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

tin^EC40 is partially rescued by Scer\GAL4^twi.PB/tin^UAS.Δ43-123/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

Not rescued by

tin^EC40 is not rescued by Scer\GAL4^twi.PB/tin^UAS.ΔHD/Scer\GAL4^Mef2.PR

(Ranganayakulu et al., 1998)

Comments

tin^{Scer\UAS.Δ43-123} fully rescues visceral mesoderm defect but only partially rescues cardiac mesoderm defects of tin^EC40.

(Ranganayakulu et al., 1998)

Images (0)

Mutant

Wild-type

Stocks (1)

Bloomington

78560

tin^EC40/TM3, P{HZR+6.8Xb}JG1, Sb¹

Notes on Origin

Discoverer

Mohler and Pardue.

(Bodmer, 1993)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (2)

Reported As

Symbol Synonym

tin^EC40

(Lovato et al., 2023, Wu et al., 2019, Schwarz et al., 2018, Sánchez-Higueras and Hombría, 2016, Schaub et al., 2015, Boukhatmi et al., 2012, Park et al., 2011, Qian et al., 2011, Liu et al., 2009, Qian and Bodmer, 2009, Qian et al., 2008, Ryan et al., 2007, Zaffran et al., 2006)

tin^ec40

(Ryu et al., 2011, Miskolczi-McCallum et al., 2005)

Name Synonyms

Secondary FlyBase IDs

References (31)

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