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General Information
Symbol
Dmel\trxB11
Species
D. melanogaster
Name
FlyBase ID
FBal0032908
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Mutagen
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
deletion
Comment:

Reported as a deletion of 833 nucleotides (2817-3649 in GB:M31617) leading to a frameshift and early translation termination. In the reference sequence, the span between the deletion endpoints is 967 bases.

Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

trxB11 is associated with an 833bp deletion. It could encode truncated proteins consisting of 8.6% and 17.7% of the N terminus of TRXI and TRXII respectively.

Deletion that truncates the 3726 residue protein after residue 658.

Frameshift deletion in the amino terminal portion that terminates the open reading frame after 17.5% of its length.

Small frame-shifting deletion in the common exon 3.

800bp deletion within the coding region.

Deletion of DNA, leading to a frameshift that terminates the open reading frame after the first 658 residues.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Double heterozygous stuxnetd77;trxB11 females display tergite boundary defects that are not observed in stuxnetd77 heterozygote females. stuxnetd77/Y;trxB11/+ males are lethal.

Double heterozygous stuxnet2;trxB11 females display tergite boundary defects that are not observed in stuxnet2 single heterozygote females.

27.9% of heterozygous males show anterior transformation of segment A6 to A5.

Homozygous embryos show defects in cuticle patterning: 33% lack all the denticle belts and 3% show transformation of one segment to another (although denticle belts are present).

The brains of trxJY16/trxB11 and trxE3/trxB11 larvae show a severe reduction in BrdU incorporation compared to wild type. Brain squashes are characterised by global chromatin decondensation, resembling 'prophase-like' chromatin.

trx1/trxB11 flies show transformations of wing to haltere; 49% show strong transformation, 34% show weak transformation and 16% show no transformation.

Heterozygotes show mild anterior transformations of the fifth, sixth, and seventh abdominal segments.

4.5% of heterozygous adults show at least one transformation phenotype.

Abdominal segment of heterozygous males is transformed anteriorly. Haltere is normal.

Heterozygotes do not have an apical bristle on the T3 legs.

Homozygous embryos exhibit altered gut morphology by stage 15. Loss of trx in enF4 flies causes female sterility.

Individuals die before or upon hatching.

Abnormal midgut morphology: only the second midgut constriction appears in its normal position. The first and third constrictions appear posterior to their wild-type positions.

Reduction in size of all ventral setal belts, third thoracic ventral setal belt has the appearance of the second thoracic ventral setal belt and the posterior abdominal ventral setal belts show transformation to an identity like that of a normal second thoracic ventral setal belt.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference

trxB11 has visible | dominant | homeotic | male phenotype, enhanceable by scf1/scf[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Asx3/Asx[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by AsxXF23/Asx[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by E(Pc)[+]/E(Pc)1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by E(z)[+]/E(z)5

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Psc[+]/Psc1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Sce[+]/SceD1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by ScmD1/Scm[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by ScmM56/Scm[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Su(z)21/Su(z)2[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Su(z)4[+]/Su(z)41

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Su(z)6[+]/Su(z)61

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Su(z)7[+]/Su(z)71

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Trl[+]/TrlR85

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by mxc[+]/mxcG48

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by sxc[+]/sxc1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by lidk06801/lid[+]

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by lawcp1/lawc[+]

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by lawc[+]/lawcEF520

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by Df(1)RA2/+

ash117, trxB11 has visible | homeotic phenotype, enhanceable by lawcEF520

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by lawcp1/lawc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by lawc[+]/lawcEF520

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, enhanceable by Df(1)RA2/+

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by mod(mdg4)[+]/mod(mdg4)ul

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by mod(mdg4)[+]/mod(mdg4)T6

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, enhanceable by mod(mdg4)T16/mod(mdg4)[+]

mod(mdg4)T6, trxB11 has visible | dominant | homeotic phenotype, enhanceable by ash1[+]/ash117

NOT Enhanced by
Statement
Reference

trxB11 has visible | dominant | homeotic phenotype, non-enhanceable by lawcp1

trxB11 has visible | dominant | homeotic phenotype, non-enhanceable by lawcEF520

trxB11 has visible | dominant | homeotic phenotype, non-enhanceable by lawc+10

trxB11 has visible | dominant | homeotic phenotype, non-enhanceable by Df(1)RA2

trxB11 has visible | homeotic phenotype, non-enhanceable by lawcp1

Suppressed by
Statement
Reference

ash117, trxB11/trx[+] has visible | dominant phenotype, suppressible | partially by His2Av05146

ash117, trxB11/trx[+] has visible | dominant phenotype, suppressible | partially by His2Av810

trxB11/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has visible | homeotic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

ash1[+]/ash117, trxB11 has visible | dominant | homeotic phenotype, suppressible by dom14/dom[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by dom14/dom[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by Pc3/Pc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by Pcl7/Pcl[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by Psc[+]/PscArp1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by ScmSuz302/Scm[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by esc10/esc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by esc21/esc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by esc5/esc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by esc9/esc[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by mxc[+]/mxc1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by mxc[+]/mxcM1

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by ph-d[+]/ph-d503

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by phob/pho[+]

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by sxc[+]/sxc4

ash117, trxB11/trx[+] has visible | dominant | homeotic phenotype, suppressible by sxc[+]/sxc5

NOT suppressed by
Statement
Reference
Enhancer of
Statement
Reference
Other
Statement
Reference

stxd77, trxB11/trx[+] has lethal phenotype

stx2, trxB11/trx[+] has visible | adult stage phenotype

ash1[+]/ash117, trxB11 has visible | homeotic phenotype

Phenotype Manifest In
Enhanced by
Statement
Reference

trxB11 has adult abdominal segment 6 | male phenotype, enhanceable by scf1/scf[+]

trxB11 has adult abdominal segment 5 | ectopic | male phenotype, enhanceable by scf1/scf[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Asx3/Asx[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by AsxXF23/Asx[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by E(Pc)[+]/E(Pc)1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by E(z)[+]/E(z)5

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Psc[+]/Psc1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Sce[+]/SceD1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by ScmD1/Scm[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by ScmM56/Scm[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Su(z)21/Su(z)2[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Su(z)4[+]/Su(z)41

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Su(z)6[+]/Su(z)61

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Su(z)7[+]/Su(z)71

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by Trl[+]/TrlR85

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by mxc[+]/mxcG48

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by sxc[+]/sxc1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, enhanceable by lidk06801/lid[+]

brm2, trxB11/trx[+] has haltere phenotype, enhanceable by lawcp1/lawc[+]

brm2, trxB11/trx[+] has metathoracic leg phenotype, enhanceable by lawcp1/lawc[+]

ash1[+]/ash117, trxB11 has haltere phenotype, enhanceable by lawcp1/lawc[+]

ash1[+]/ash117, trxB11 has metathoracic leg phenotype, enhanceable by lawcp1/lawc[+]

ash117, trxB11/trx[+] has haltere phenotype, enhanceable by lawcp1/lawc[+]

ash117, trxB11/trx[+] has metathoracic leg phenotype, enhanceable by lawcp1/lawc[+]

ash117, trxB11/trx[+] has haltere phenotype, enhanceable by lawc[+]/lawcEF520

mod(mdg4)T6, trxB11 has haltere phenotype, enhanceable by ash1[+]/ash117

NOT Enhanced by
Statement
Reference

trxB11 has adult abdominal segment 5 phenotype, non-enhanceable by Hsc70-403550/Hsc70-4[+]

trxB11 has adult abdominal segment 5 phenotype, non-enhanceable by Hsc70-4[+]/Hsc70-454.1

trxB11 has adult abdominal segment 5 phenotype, non-enhanceable by Hsc70-4II-R35-R7/Hsc70-4[+]

trxB11 has phenotype, non-enhanceable by Asx3

trxB11 has phenotype, non-enhanceable by Asx8

trxB11 has phenotype, non-enhanceable by trr1

Suppressed by
Statement
Reference

ash117, trxB11/trx[+] has metathoracic leg phenotype, suppressible | partially by His2Av810

trxB11/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B87Bg-6

trxB11/trx1 has wing phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxB11/trx1 has haltere | ectopic phenotype, suppressible | partially by Pp1-87B[+]/Pp1-87B1

trxB11 has adult abdominal segment 5 phenotype, suppressible by Hsc70-4+t14/Hsc70-4[+]

trxB11 has adult abdominal segment 5 phenotype, suppressible by Hsc70-4195/Hsc70-4[+]

trxB11 has adult abdominal segment 5 phenotype, suppressible by Hsc70-4PB2/Hsc70-4[+]

ash1[+]/ash117, trxB11 has abdominal segment 6 phenotype, suppressible by dom14/dom[+]

ash117, trxB11/trx[+] has abdominal segment 6 phenotype, suppressible by dom14/dom[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by Pc3/Pc[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by Pcl7/Pcl[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by Psc[+]/PscArp1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by ScmSuz302/Scm[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by esc10/esc[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by esc21/esc[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by esc5/esc[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by esc9/esc[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by mxc[+]/mxc1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by mxc[+]/mxcM1

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by ph-d[+]/ph-d503

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by phob/pho[+]

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by sxc[+]/sxc4

ash117, trxB11/trx[+] has adult metathoracic segment phenotype, suppressible by sxc[+]/sxc5

NOT suppressed by
Statement
Reference

trxB11 has phenotype, non-suppressible by Asx3

trxB11 has phenotype, non-suppressible by Asx8

trxB11 has phenotype, non-suppressible by trr1

Enhancer of
Statement
Reference

trxB11/trx[+] is an enhancer of pigment cell phenotype of w4.Ubx.bxd:pbx.N

trxB11 is an enhancer of haltere phenotype of AsxP1

trxB11 is an enhancer of pigment cell phenotype of wUbx.bxd:pbx.N

trxB11, trx[+], ash1[+], ash117 is an enhancer of arista phenotype of lawcp1

trxB11 is an enhancer of haltere phenotype of mod(mdg4)ul

trxB11, trx[+], ash1[+], ash117 is an enhancer of phenotype of mod(mdg4)ul

NOT Enhancer of
Statement
Reference

trxB11 is a non-enhancer of phenotype of w4.Ubx.bxd:pbx.ΔBC1

trxB11 is a non-enhancer of phenotype of wUbx.bxd:pbx.ΔBC

Other
Statement
Reference

stx2, trxB11/trx[+] has tergite phenotype

stxd77, trxB11/trx[+] has tergite phenotype

Additional Comments
Genetic Interactions
Statement
Reference

scf1/+ significantly enhances the penetrance of the A6 to A5 transformation phenotype that is seen in trxB11/+ males.

ash117 trxB11 double heterozygotes show transformations of third leg into second leg; an apical bristle appears on the third leg in 66% of cases. The frequency of the appearance of an apical bristle on the third leg decreases to 37% in ash117 trxB11 His2Av05146 triple heterozygotes and to 29% in ash117 trxB11 His2Av810 triple heterozygotes.

Pp1-87B87Bg-6 dominantly suppresses the transformation of wing to haltere seen in trx1/trxB11 flies; 28% show strong transformation, 29% show weak transformation and 43% show no transformation.

Pp1-87B1 dominantly suppresses the transformation of wing to haltere seen in trx1/trxB11 flies; 27% show strong transformation, 24% show weak transformation and 49% show no transformation.

Expression of Pp1-87BScer\UAS.T:Ivir\HA1 under the control of Scer\GAL4arm.PS has no effect on the transformation of wing to haltere seen in trx1/trxB11 flies.

trxB11 Hsc70-4I-R35-R7 double heterozygotes show a reduction in the number of sex comb teeth.

The abdominal segment A6 to A5 transformation of ash117/+, trxB11/+ is dominantly suppressed by dom14.

Double heterozygotes with Df(2L)Mdh, Df(2R)or-BR6, Df(3L)Ar14-8, Df(2L)cl-h3, Df(2R)Pu-D17, Df(3L)vin6, lidk06801, E(Pc)1, Psc1, SceD1, TrlR85, Su(z)71, Su(z)61, sxc1, Su(z)41, AsxXF23, Asx3 or Asx13 show T3 to T2 transformations. 52.1% of trxB11 ash117 double heterozygotes show T3 to T2 transformations. The penetrance of this phenotype is enhanced by one copy of lidk06801, E(Pc)1, Su(z)21, Psc1, AsxXF23, Asx3, mxcG48, sxc1, SceD1, E(z)5, TrlR85, Su(z)41, Su(z)71, Su(z)61, ScmD1 or ScmM56 and suppressed by one copy of Pc3, ph-d503, Pcl7, phob, mxcM1, mxc1, esc5, esc9, esc10, esc21, sxc4, sxc5, PscArp1 or ScmSuz302.

In combination with AsxP1, the haltere is much enlarged over that seen for AsxP1 alone, T3 is partially transformed towards T2. These phenotypes have a penetrance of close to 100%.

trr1 neither enhances nor suppresses trxB11 adult homeotic phenotypes.

The frequency of homeotic transformations in heterozygotes is not enhanced by lawcp1, lawcEF520, lawc+10 or Df(1)RA2. The frequency and severity of homeotic transformations (such as haltere to wing or third leg to second leg transformations) in trxB11 ash117 double heterozygotes is dominantly enhanced by lawcp1. The enhancement is stronger in a lawcp1 maternal background. The arista to leg transformation characteristic of lawcp1 is also enhanced in these flies. The frequency of homeotic transformations in trxB11 ash117 double heterozygotes is dominantly enhanced by lawcEF520 or Df(1)RA2. lawcp1/lawcEF520 females are viable. However, in combination with trxB11, lawcp1/lawcEF520 results in lethality. lawcp1/Df(1)RA2 females are viable. However, in combination with trxB11, lawcp1/Df(1)RA2 results in lethality.

trxB11 shows a transformation of haltere to wing in double heterozygous combination with either mod(mdg4)T6, mod(mdg4)ul or mod(mdg4)T16. 14.1% of trxB11 ash117 double heterozygous adults show a homeotic phenotype. This is increased to 77.7% if the flies are also heterozygous for mod(mdg4)T16, 50.4% if the flies are also heterozygous for mod(mdg4)ul and 32.6% if the flies are also heterozygous for mod(mdg4)T6. Dominantly enhances the effect of mod(mdg4)ul on the y2 phenotype. The combination ash117 trxB11 dominantly enhances the effect of mod(mdg4)ul on the y2 phenotype.

Xenogenetic Interactions
Statement
Reference

Expression of Hsap\MLLScer\UAS.C.T:Zzzz\FLAG under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 partially rescues the cuticle pattern defects seen in trxB11 homozygous embryos: 9% of embryos have a wild-type cuticle pattern.

Expression of Hsap\MLL::Hsap\AFF1Scer\UAS.cMa under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 fails to rescue the cuticle pattern defects seen in trxB11 homozygous embryos.

Complementation and Rescue Data
Rescued by
Comments

Mutant phenotype can be rescued by a cosPneo element carrying the entire 25kb trx transcription unit.

Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments

See also Mazo et al., PNAS 87: 2112--2116, and Breen and Harte, Mech Dev 35: 113--127.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (2)
References (32)