trxB11 is associated with an 833bp deletion. It could encode truncated proteins consisting of 8.6% and 17.7% of the N terminus of TRXI and TRXII respectively.
Deletion that truncates the 3726 residue protein after residue 658.
Frameshift deletion in the amino terminal portion that terminates the open reading frame after 17.5% of its length.
Small frame-shifting deletion in the common exon 3.
800bp deletion within the coding region.
Deletion of DNA, leading to a frameshift that terminates the open reading frame after the first 658 residues.
ash117 trxB11 double heterozygotes show transformations of third leg into second leg; an apical bristle appears on the third leg in 66% of cases. The frequency of the appearance of an apical bristle on the third leg decreases to 37% in ash117 trxB11 His2Av05146 triple heterozygotes and to 29% in ash117 trxB11 His2Av810 triple heterozygotes.
Double heterozygotes with Df(2L)Mdh, Df(2R)or-BR6, Df(3L)Ar14-8, Df(2L)cl-h3, Df(2R)Pu-D17, Df(3L)vin6, lidk06801, E(Pc)1, Psc1, SceD1, TrlR85, Su(z)71, Su(z)61, sxc1, Su(z)41, AsxXF23, Asx3 or Asx13 show T3 to T2 transformations. 52.1% of trxB11 ash117 double heterozygotes show T3 to T2 transformations. The penetrance of this phenotype is enhanced by one copy of lidk06801, E(Pc)1, Su(z)21, Psc1, AsxXF23, Asx3, mxcG48, sxc1, SceD1, E(z)5, TrlR85, Su(z)41, Su(z)71, Su(z)61, ScmD1 or ScmM56 and suppressed by one copy of Pc3, ph-d503, Pcl7, phob, mxcM1, mxc1, esc5, esc9, esc10, esc21, sxc4, sxc5, PscArp1 or ScmSuz302.
The frequency of homeotic transformations in heterozygotes is not enhanced by lawcp1, lawcEF520, lawc+10 or Df(1)RA2. The frequency and severity of homeotic transformations (such as haltere to wing or third leg to second leg transformations) in trxB11 ash117 double heterozygotes is dominantly enhanced by lawcp1. The enhancement is stronger in a lawcp1 maternal background. The arista to leg transformation characteristic of lawcp1 is also enhanced in these flies. The frequency of homeotic transformations in trxB11 ash117 double heterozygotes is dominantly enhanced by lawcEF520 or Df(1)RA2. lawcp1/lawcEF520 females are viable. However, in combination with trxB11, lawcp1/lawcEF520 results in lethality. lawcp1/Df(1)RA2 females are viable. However, in combination with trxB11, lawcp1/Df(1)RA2 results in lethality.
trxB11 shows a transformation of haltere to wing in double heterozygous combination with either mod(mdg4)T6, mod(mdg4)ul or mod(mdg4)T16. 14.1% of trxB11 ash117 double heterozygous adults show a homeotic phenotype. This is increased to 77.7% if the flies are also heterozygous for mod(mdg4)T16, 50.4% if the flies are also heterozygous for mod(mdg4)ul and 32.6% if the flies are also heterozygous for mod(mdg4)T6. Dominantly enhances the effect of mod(mdg4)ul on the y2 phenotype. The combination ash117 trxB11 dominantly enhances the effect of mod(mdg4)ul on the y2 phenotype.
Expression of Hsap\MLLScer\UAS.C.T:Zzzz\FLAG under the control of Scer\GAL4mat.αTub67C.T:Hsim\VP16 partially rescues the cuticle pattern defects seen in trxB11 homozygous embryos: 9% of embryos have a wild-type cuticle pattern.
Mutant phenotype can be rescued by a cosPneo element carrying the entire 25kb trx transcription unit.