The extra veins in the adult wing induced by rhohs.PSt are strongly suppressed by the addition of ectopic mskScer\UAS.cLa (under the control of Scer\GAL4en-e16E). rhohs.PSt mskScer\UAS.cLa (under the control of Scer\GAL4en-e16E) wings are significantly larger than rhohs.PSt wings, despite no decrease in cell density, indicating an increase in cell division.
When rhohs.PSt; spihs.PG flies are exposed to heat shock, no effect of elevated spihs.PG expression is seen beyond that attributable to rhohs.PSt misexpression. When Shs.PK; rhohs.PSt; spihs.PG are exposed to during early pupariation (mostly between 6 and 12 hours after puparium formation (APF)) a phenotype is seen that is stronger than Shs.PK; rhohs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by Shs.PK ; rhohs.PSt, a sharp pattern of well defined forked longitudinal veins is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF.
The ubiquitous expression of rhohs.PSt under heatshock leads to a partial suppression of the spi1 loss of midline glial cell phenotype. No significant suppression of the axon tract phenotype is seen, however longitudinal tracts are thinner in these embryos. (this effect is not seen when rhohs.PSt is added to wild-type embryos). Commissure separation in rhohs.PSt,spi1 is not improved. The ubiquitous expression of rhohs.PSt under heatshock has no effect on SIIN or Egfrflb-2E07 embryos.