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General Information
Symbol
Dmel\rhohs.PSt
Species
D. melanogaster
Name
heat shock construct of Sturtevant
FlyBase ID
FBal0033082
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
hs-rho
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Carried in construct
Cytology
Nature of the lesion
Statement
Reference
An Hsp70 promoter drives expression of a wild-type rho cDNA.
Allele components
Product class / Tool use(s)
Regulatory region(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference
Overexpression of rhohs.PSt leads to vein hypertrophy but not an increased wing area or cell division.
When heat shock drives expression of rhohs.PSt the resulting phenotype is cyclopia in the embryonic head.
Rough eye phenotype.
Expression of rhohs.PSt using multiple heat shocks during larval stages leads to failure of photoreceptor differentiation and a dorsoventral stripe of scar tissue in the adult eye.
Embryos overexpressing rhohs.PSt show a 'cyclops' phenotype in which the optic lobes are enlarged and show dorsal fusion.
Heat shock driven misexpression of rhohs.PSt produces a number of wing phenotypes including, blisters separating the dorsal and ventral surfaces of the wing, ectopic crossveins, an ectopic vein spur running between wing veins L3 and L4 near the margin, and a thickened L3 vein. All phenotypes are seen if heatshock occurs within 0-30 hours after puparium formation (APF).
Ubiquitous and sustained expression of rhohs.PSt under heatshock, results in supernumerary midline glial cells.
Overexpression of rhohs.PSt results in dramatically enlarged wing vein tissue.
Causes complex deformity of the embryonic visual system. Optic lobe is enlarged and invaginates earlier and at a more dorsomedial position than in wild type. The differentiation of Bolwig's organ is also abnormal; in many cases it does not separate from the optic lobe and does not form axons. Heat shock activation between 5 and 7 hours postfertilization has the strongest effect.
Shows extra wing vein material phenotype, even at 25oC.
Causes dorsalization of the egg.
Heat shocked embryos show an increase of 1 or 2 chordotonal organs in the lateral pentascolopidial chordotonal organs (LCh5) and in the ventral cluster.
The number of midline glia cells is increased to an average of 7 cells per segment in homozygous embryos.
Ectopic expression of rhohs.PSt in the disc and pupal wing results in the differentiation of ectopic and thicker veins.
Heat induced expression causes an increase in the number of chordotonal neurons.
The number of MGA and MGM cells can be increased by the ectopic expression of rho. Onset of midline glial cell death is shifted earlier, to stage 12 rather than stage 13, in these embryos.
Heat shock at germband extension leads to the production of stretch receptor organ precursor cells and heat inductions during wing vein development leads to the formation of ectopic veins.
Eggs laid after heat shock have dorsalized eggshells and give rise to dorsalized embryos, with expansion of dorsal structures at the expense of ventral. In some embryos stronger dorsalization was detected in the anterior region than the posterior, similar to the mutant phenotype in fs(1)K10 mutants. The large percentage of nonhatching embryos reflects failure of fertilization and dorsalization of the embryo.
External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement
Reference
rhohs.PSt has visible phenotype, enhanceable by ed[+]/ed1X5
Shs.PK, rhohs.PSt has visible phenotype, enhanceable by spihs.PG
Other
Phenotype Manifest In
Enhanced by
Statement
Reference
rhohs.PSt has eye phenotype, enhanceable by ed[+]/ed1X5
Shs.PK, rhohs.PSt has wing phenotype, enhanceable by spihs.PG
rhohs.PSt has phenotype, enhanceable by bs03267
rhohs.PSt has wing vein phenotype, enhanceable by tkv1/tkv8
rhohs.PSt has wing vein | ectopic phenotype, enhanceable by bsba
rhohs.PSt has wing vein | ectopic phenotype, enhanceable by bs11
rhohs.PSt has wing vein | ectopic phenotype, enhanceable by bs2
rhohs.PSt has phenotype, enhanceable by bsPx
NOT Enhanced by
Statement
Reference
rhohs.PSt has phenotype, non-enhanceable by spihs.PG
Suppressed by
Statement
Reference
NOT suppressed by
Statement
Reference
rhohs.PSt has phenotype, non-suppressible by spihs.PG
Enhancer of
Statement
Reference
rhohs.PSt is an enhancer of wing phenotype of Shs.PK
NOT Enhancer of
Statement
Reference
rhohs.PSt is a non-enhancer of phenotype of Egfrflb-2E07
rhohs.PSt is a non-enhancer of phenotype of SIIN
Suppressor of
Statement
Reference
NOT Suppressor of
Statement
Reference
rhohs.PSt is a non-suppressor of phenotype of Egfrflb-2E07
rhohs.PSt is a non-suppressor of phenotype of SIIN
Other
Additional Comments
Genetic Interactions
Statement
Reference
The extra veins in the adult wing induced by rhohs.PSt are strongly suppressed by the addition of ectopic mskScer\UAS.cLa (under the control of Scer\GAL4en-e16E). rhohs.PSt mskScer\UAS.cLa (under the control of Scer\GAL4en-e16E) wings are significantly larger than rhohs.PSt wings, despite no decrease in cell density, indicating an increase in cell division.
When rhohs.PSt; spihs.PG flies are exposed to heat shock, no effect of elevated spihs.PG expression is seen beyond that attributable to rhohs.PSt misexpression. When Shs.PK; rhohs.PSt; spihs.PG are exposed to during early pupariation (mostly between 6 and 12 hours after puparium formation (APF)) a phenotype is seen that is stronger than Shs.PK; rhohs.PSt alone. Also, in contrast to the diffuse ectopic veins induced by Shs.PK ; rhohs.PSt, a sharp pattern of well defined forked longitudinal veins is observed. A phenotype can also be seen in the eye, in which cell death is localised at the anterior part of the eye, when heat shock is provided between 0 and 6 hours APF.
The ubiquitous expression of rhohs.PSt under heatshock leads to a partial suppression of the spi1 loss of midline glial cell phenotype. No significant suppression of the axon tract phenotype is seen, however longitudinal tracts are thinner in these embryos. (this effect is not seen when rhohs.PSt is added to wild-type embryos). Commissure separation in rhohs.PSt,spi1 is not improved. The ubiquitous expression of rhohs.PSt under heatshock has no effect on SIIN or Egfrflb-2E07 embryos.
Dorsalization of the egg caused by rhohs.PSt is suppressed by halving the dose of spi.
Homozygous emc1 clones carrying rhohs.PSt differentiate extra veins and thicker veins. Clones located in both surfaces of the wing have a stronger phenotype than those located in only one surface.
The differentiation of thicker veins seen in rhohs.PSt is strongly increased in combination with tkv1/tkv8, while the rhohs.PSt ectopic vein phenotype is suppressed in combination with dpps4/dpps8.
Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Partially rescues
Comments
The ubiquitous expression of rhohs.PSt under heatshock is sufficient to suppress the loss of midline glial cell seen in rhoPΔ38 embryos to wild-type. Commissure separation in rhohs.PSt,rhoPΔ38 is not improved.
Images (0)
Mutant
Wild-type
Stocks (0)
Notes on Origin
Discoverer
Comments
Comments
Generated by Sturtevant, Roark and Bier.
External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (6)
Reported As
Symbol Synonym
rhohs.PSt
vehs.IC
vehs.PS
vehs.PSt
Name Synonyms
heat shock construct of Sturtevant
Secondary FlyBase IDs
  • FBal0092454
References (29)