Allele Dmel\w^BR.BR

General Information
Symbol	Dmel\w^BR.BR	Species	D. melanogaster
Name		FlyBase ID	FBal0033129
Feature type	allele	Associated gene	Dmel\w

Allele class
Mutagen	in vitro construct - regulatory fusion
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class
Mutagen	in vitro construct - regulatory fusion (Roseman et al., 1993, Roseman et al., 1995)
Mutations Mapped to the Genome

Type Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	w^+mET (Roseman et al., 1993)
Nature of the lesion	Statement Reference w^+mC minigene, which contains approximately 300bp of 5' flanking and 630bp of 3' flanking DNA, is flanked by two 430bp of su(Hw) binding region (gypsy sequences between nucleotides 647 and 1077). (Roseman et al., 1995) Construct: w^+mC minigene altered to include the su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134) upstream of the w coding region, upstream of a second su(Hw) binding region. (Roseman et al., 1993)
Carried in construct	P{SUPor-P-lt} (Giraldo et al., 2003, Roseman et al., 1993, Bhadra et al., 1999, Roseman et al., 2001, Roseman et al., 1995, Roseman et al., 1995)
Cytology
Phenotypic Data
Phenotypic Class
	eye color defective (Giraldo et al., 2003, Roseman et al., 2001) visible \| recessive (Roseman et al., 1993)
Phenotype Manifest In
	pigment cell (Roseman et al., 1993, Giraldo et al., 2003, Roseman et al., 2001)
Detailed Description
	Statement Reference Eye color: yellow (Roseman et al., 2001) The w^BR.BR mutation in X insertion lines shows complete dosage compensation. All autosomal insertion lines show some degree of dosage compensation, results suggest that the mini-w gene surrounded by su(Hw) binding region has an enhanced ability to dosage compensate at autosomal locations. In a su(Hw)^- mutant background compensation of autosomal insertion lines is incomplete, dosage compensation on the X chromosome is largely unaffected. Lack of hypercompensation of X insertion lines may be consequence of the sequences into which the insertion was integrated. (Roseman et al., 1995) Eye colour: yellow, in the majority of flies; indistinguishable from that of w^E.BR.BR. (Roseman et al., 1993)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has eye color defective phenotype, enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
NOT Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.BR has eye color defective phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has eye color defective phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has eye color defective phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has eye color defective phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has eye color defective phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has eye color defective phenotype, non-enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
NOT suppressed by
Statement Reference w^BR.BR has eye color defective phenotype, non-suppressible by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has eye color defective phenotype, non-suppressible by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
Phenotype Manifest In
Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by Pc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has pigment cell phenotype, enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
NOT Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.BR has pigment cell phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.BR has pigment cell phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has pigment cell phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.BR has pigment cell phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has pigment cell phenotype, non-enhanceable by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has pigment cell phenotype, non-enhanceable by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
NOT suppressed by
Statement Reference w^BR.BR has pigment cell phenotype, non-suppressible by esc^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001) w^BR.BR has pigment cell phenotype, non-suppressible by Scm^{hs.T:ZnF-su(Hw),T:Zzzz\FLAG} (Roseman et al., 2001)
Additional Comments
Genetic Interactions
Statement Reference Expression of w^BR.BR is reduced (in a background of reduced su(Hw)⁺) by the expression of tethered Pc, Scm or esc. In the case of Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, the effect is seen without the use of heat shock. For Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} and esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} heat shock must be applied daily. In the case of esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} the effect is dependent on the site of insertion of P{SUPor-P-lt}, in that is seen for P{SUPor-P-lt}43B but not for P{SUPor-P-lt}28C. (Roseman et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	w^BR.BR
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Giraldo et al., 2003, Nucleic Acids Res. 31(21): 6290--6305 Functional dissection of the mouse tyrosinase locus control region identifies a new putative boundary activity. [FBrf0191510] Roseman et al., 2001, Genetics 158(1): 291--307 Long-range repression by multiple Polycomb Group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila. [FBrf0135809] Bhadra et al., 1999, Genetics 152(1): 249--268 Role of the male specific lethal (msl) genes in modifying the effects of sex chromosomal dosage in Drosophila. [FBrf0108144] Roseman et al., 1995, Development 121(11): 3573--3582 A Drosophila insulator protein facilitates dosage compensation of the X chromosome mini-white gene located at autosomal insertion sites. [FBrf0084324] Roseman et al., 1995, Genetics 141(3): 1061--1074 A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. [FBrf0084323] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161]

Allele Dmel\wBR.BR

Allele Dmel\w^BR.BR