Allele Dmel\w^BR.E.BR

General Information
Symbol	Dmel\w^BR.E.BR	Species	D. melanogaster
Name		FlyBase ID	FBal0033130
Feature type	allele	Created / Updated	2006-08-20/2006-08-20
Associated gene	Dmel\w

Allele class
Mutagen	in vitro construct \| regulatory fusion
Nature of the Allele
Allele class
Mutagen	in vitro construct \| regulatory fusion (Roseman et al., 1993)
Mapped Features and Mutations
Type Symbol & Location Additional Notes References
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype	w^+mET (Roseman et al., 1993)
Nature of the lesion	Statement Reference Construct: w^+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90), upstream of the w coding region, upstream of a second su(Hw) binding region. (Roseman et al., 1993) Two gypsy\su(Hw)BR binding sites flank the w^+mET sequences to insulate the w gene from chromosomal position effects. (Roseman et al., 1995) P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter. (Bhadra et al., 1999)
Assay mode	In transgenic Drosophila (intraspecific) (Roseman et al., 1993)
Carried in construct	P{SUPor-P} (Maggert and Golic, 2002, Roseman et al., 1993, Lankenau et al., 2000, Kurenova et al., 1998, Bhadra et al., 1999, Soldatov et al., 1999, Roseman et al., 2001, Roseman et al., 1995, Mallin et al., 1998) P{SUPor-P.PRE#2} (Mallin et al., 1998) P{SUPor-P.PRE#6} (Mallin et al., 1998)
Cytology
Phenotypic Data
Phenotypic Class
	visible \| recessive (Roseman et al., 1993) eye color defective (Kurenova et al., 1998, Roseman et al., 2001)
Phenotype Manifest In
	pigment cell (Roseman et al., 1993, Kurenova et al., 1998, Roseman et al., 2001)
Detailed Description
	Statement Reference Eye colour: highly pigmented, in the majority of flies. (Roseman et al., 1993) Eye colour: Orange to dark orange (depending on insertion site). (Kurenova et al., 1998) Eye colour: flies carrying w^BR.E.BR have red eyes. (Soldatov et al., 1999) Eye color: red (Roseman et al., 2001)
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
Statement Reference w^BR.E.BR, z^v77h has eye color defective phenotype, enhanceable by e(y)1¹ (Soldatov et al., 1999) su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has eye color defective phenotype, enhanceable by e(y)1¹ (Soldatov et al., 1999) e(y)1¹, w^BR.E.BR, z^v77h has eye color defective phenotype, enhanceable by su(Hw)^V/su(Hw)² (Soldatov et al., 1999) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001)
NOT Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, non-enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, non-enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001)
Suppressed by
Statement Reference Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has eye color defective phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001)
Other
Statement Reference w^BR.E.BR, z^v77h has eye color defective phenotype (Soldatov et al., 1999) su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has eye color defective phenotype (Soldatov et al., 1999)
Phenotype Manifest In
Enhanced by
Statement Reference w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by e(y)1¹ (Soldatov et al., 1999) su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by e(y)1¹ (Soldatov et al., 1999) e(y)1¹, w^BR.E.BR, z^v77h has pigment cell phenotype, enhanceable by su(Hw)^V/su(Hw)² (Soldatov et al., 1999) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001)
NOT Enhanced by
Statement Reference su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001) su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, non-enhanceable by Scm^{hs.ΔSPM.T:su(Hw)-ZnF,T:Zzzz\FLAG} (Roseman et al., 2001)
Suppressed by
Statement Reference Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)⁷/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001) esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, su(Hw)^V/su(Hw)^E8, w^BR.E.BR has pigment cell phenotype, suppressible by ph-p⁴⁰⁹ (Roseman et al., 2001)
Other
Statement Reference w^BR.E.BR, z^v77h has pigment cell phenotype (Soldatov et al., 1999) su(Hw)^V/su(Hw)², w^BR.E.BR, z^v77h has pigment cell phenotype (Soldatov et al., 1999)
Additional Comments
Genetic Interactions
Statement Reference The eye colour is not affected if w^BR.E.BR flies are also carrying e(y)1¹ and z^v77h. Flies that are also carrying e(y)1¹, z^v77h and su(Hw)²/su(Hw)^V have reduced eye pigmentation. (Soldatov et al., 1999) Expression of w^BR.E.BR is reduced (in a background of reduced su(Hw)⁺) by the expression of tethered Pc, Scm or esc. In the case of Pc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG}, the effect is seen without the use of heat shock. For Scm^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} and esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} heat shock must be applied daily. In the case of esc^{hs.T:su(Hw)-ZnF,T:Zzzz\FLAG} the effect is dependent on the site of insertion of P{SUPor-P}. ph-p⁴⁰⁹ completely suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Pc, and substantially suppresses the strong silencing of w^BR.E.BR in P{SUPor-P}22F caused by tethered Scm or esc. (Roseman et al., 2001)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 2223 )
Bloomington	12875 y¹ P{SUPor-P}KG00797 12876 y¹ P{SUPor-P}KG02934 12878 y¹ P{SUPor-P}KG03323 12880 y¹ P{SUPor-P}KG00735 12883 y¹ P{SUPor-P}KG02856 12884 y¹ P{SUPor-P}KG00777 12885 y¹ P{SUPor-P}KG02704 12887 y¹ P{SUPor-P}KG02708 12889 y¹ P{SUPor-P}KG03295 12969 y¹ P{SUPor-P}KG00970 12984 y¹ P{SUPor-P}KG02394 12987 y¹ P{SUPor-P}KG02802
	More stocks available...
Notes on Origin
Discoverer

Comments
	Three insertion lines carrying this allele show variegation for w, and behave as though the insertions are heterochromatic. In situ hybridization confirms that the insertion in one of these lines is in the second chromosome centric heterochromatin. In a su(Hw) mutant background the variegating phenotype of these insertions becomes more pronounced, and the su(Hw) mutation acts dominantly in this interaction. (Roseman et al., 1993)
Synonyms & Secondary IDs ( 1 )
Reported As
Symbol Synonym	w^BR.E.BR
Name Synonym
Secondary FlyBase IDs

References ( 9 )
Research paper	Maggert and Golic, 2002, Genetics 162(3): 1245--1258 The Y chromosome of Drosophila melanogaster exhibits chromosome-wide imprinting. [FBrf0155500] Roseman et al., 2001, Genetics 158(1): 291--307 Long-range repression by multiple Polycomb Group (PcG) proteins targeted by fusion to a defined DNA-binding domain in Drosophila. [FBrf0135809] Lankenau et al., 2000, Chromosoma 109(1-2): 148--160 The Su(Hw) chromatin insulator protein alters double-strand break repair frequencies in the Drosophila germ line. [FBrf0128123] Bhadra et al., 1999, Genetics 152(1): 249--268 Role of the male specific lethal (msl) genes in modifying the effects of sex chromosomal dosage in Drosophila. [FBrf0108144] Soldatov et al., 1999, Molec. Cell. Biol. 19(5): 3769--3778 TAFII40 protein is encoded by the e(y)1 gene: biological consequences of mutations. [FBrf0108279] Kurenova et al., 1998, Chromosoma 107(5): 311--320 Directional gene silencing induced by a complex subtelomeric satellite from Drosophila. [FBrf0105867] Mallin et al., 1998, Genetics 148(1): 331--339 Polycomb group repression is blocked by the Drosophila suppressor of Hairy-wing [su(Hw)] insulator. [FBrf0100598] Roseman et al., 1995, Genetics 141(3): 1061--1074 A P element containing suppressor of Hairy-wing binding regions has novel properties for mutagenesis in Drosophila melanogaster. [FBrf0084323] Roseman et al., 1993, EMBO J. 12(2): 435--442 The su(Hw) protein insulates expression of the Drosophila melanogaster white gene from chromosomal position-effects. [FBrf0058161]

Allele Dmel\wBR.E.BR

Allele Dmel\w^BR.E.BR