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General Information
Symbol
Dmel\wBR.E.BR
Species
D. melanogaster
Name
FlyBase ID
FBal0033130
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
P{SUPor-P}
Allele class
Nature of the Allele
Allele class
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Progenitor genotype
Cytology
Nature of the lesion
Statement
Reference

P{SUPor-P-lt} contains a mini-w gene that has two flanking gypsy\su(Hw)BR sites, one of which is upstream of the w eye promoter.

Two gypsy\su(Hw)BR binding sites flank the w+mET sequences to insulate the w gene from chromosomal position effects.

Construct: w+mC minigene altered to include a su(Hw) binding region (all 12 binding sites, nucleotides 647 to 1077 of Marlor et al., Mol Cell Biol 6:1129-1134 ), upstream of the w eye enhancer (nucleotides -1084 to -1465 of Qian et al., Genetics 131:79-90 ), upstream of the w coding region, upstream of a second su(Hw) binding region.

Allele components
Product class / Tool use(s)
Encoded product / tool
Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

Eye color: red

Eye colour: flies carrying wBR.E.BR have red eyes.

Eye colour: Orange to dark orange (depending on insertion site).

Eye colour: highly pigmented, in the majority of flies.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Enhanced by
NOT Enhanced by
Suppressed by
Other
Phenotype Manifest In
Enhanced by
NOT Enhanced by
Suppressed by
Other
Additional Comments
Genetic Interactions
Statement
Reference

Expression of wBR.E.BR is reduced (in a background of reduced su(Hw)+) by the expression of tethered Pc, Scm or esc. In the case of Pchs.T:su(Hw)-ZnF,T:Zzzz\FLAG, the effect is seen without the use of heat shock. For Scmhs.T:su(Hw)-ZnF,T:Zzzz\FLAG and eschs.T:su(Hw)-ZnF,T:Zzzz\FLAG heat shock must be applied daily. In the case of eschs.T:su(Hw)-ZnF,T:Zzzz\FLAG the effect is dependent on the site of insertion of P{SUPor-P}. ph-p409 completely suppresses the strong silencing of wBR.E.BR in P{SUPor-P}22F caused by tethered Pc, and substantially suppresses the strong silencing of wBR.E.BR in P{SUPor-P}22F caused by tethered Scm or esc.

The eye colour is not affected if wBR.E.BR flies are also carrying e(y)11 and zv77h. Flies that are also carrying e(y)11, zv77h and su(Hw)2/su(Hw)V have reduced eye pigmentation.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (1,997)
Notes on Origin
Discoverer
Comments
Comments

Three insertion lines carrying this allele show variegation for w, and behave as though the insertions are heterochromatic. In situ hybridization confirms that the insertion in one of these lines is in the second chromosome centric heterochromatin. In a su(Hw) mutant background the variegating phenotype of these insertions becomes more pronounced, and the su(Hw) mutation acts dominantly in this interaction.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (1)
Reported As
Symbol Synonym
wBR.E.BR
Name Synonyms
Secondary FlyBase IDs
    References (9)