FlyBase Allele Report: Dmel\18w[Δ7-35]

General Information

Symbol

Dmel\18w^Δ7-35

Species

D. melanogaster

Name

FlyBase ID

FBal0033274

Feature type

allele

Associated gene

Dmel\18w

Associated Insertion(s)

Carried in Construct

Also Known As

18w^7-35

Allele class

loss of function allele

Mutagen

Nature of the Allele

Allele class

loss of function allele

(Eldon et al., 1994)

Mutagen

Delta2-3 transposase

(Eldon et al., 1994)

P-element activity

(Williams et al., 1997)

Mutations Mapped to the Genome

Type

Location

Additional Notes

References

Associated Sequence Data

DDBJ / EMBL / GenBank

DNA sequence

Protein sequence

UniProtKB/Swiss-Prot

UniProtKB/TrEMBL

Progenitor genotype

18w⁰⁰⁰⁵³

(Williams et al., 1997, Eldon et al., 1994)

Cytology

Nature of the lesion

Statement

Reference

Imprecise excision of the P{PZ} element present in 18w⁰⁰⁰⁵³, resulting in a 2.2kb deletion extending from the site of the P{PZ} insertion into the 18w open reading frame.

(Williams et al., 1997)

Deletion of the P{PZ} element and 2.2kb of genomic DNA removing approximately 2.2kb of open reading frame.

(Eldon et al., 1994)

Expression Data

Reporter Expression

Additional Information

Statement

Reference

Marker for

Reflects expression of

Reporter construct used in assay

Human Disease Associations

Disease Ontology (DO) Annotations

Models Based on Experimental Evidence ( 0 )

Disease

Evidence

References

Modifiers Based on Experimental Evidence ( 0 )

Disease

Interaction

References

Comments on Models/Modifiers Based on Experimental Evidence ( 0 )

Phenotypic Data

Phenotypic Class

abnormal immune response | recessive

(Williams et al., 1997)

lethal

(Yagi et al., 2010)

lethal (with 18w^k02701)

(Kahsai et al., 2016)

lethal (with Df(2R)BSC594)

(Kahsai et al., 2016)

lethal | recessive

(Chiang and Beachy, 1994)

lethal - all die before end of larval stage | recessive

(Eldon et al., 1994)

partially lethal - majority die (with 18w^k02701)

(Yagi et al., 2010)

some die during larval stage | recessive

(Eldon et al., 1994)

viable (with Df(2R)017)

(Ligoxygakis et al., 2002)

Phenotype Manifest In

antenna

(Eldon et al., 1994)

haltere

(Eldon et al., 1994)

leg

(Eldon et al., 1994)

wing

(Eldon et al., 1994)

Detailed Description

Statement

Reference

18w^Δ7-35/Df(2R)017 flies that have been challenged with vesicular stomatitis virus show levels of viral replication that are not significantly different to that seen in wild-type controls.

(Nakamoto et al., 2012)

The 18w^Δ7-35/Df(2R)017 combination results in viable adults (although in numbers significantly lower than the expected Mendelian ratios).

18w^Δ7-35/Df(2R)017 flies show normal survival rates in response to infection with fungae or Gram-negative bacteria.

(Ligoxygakis et al., 2002)

Only 56% of homozygous 18w^Δ7-35 third instar larvae are still alive 24 hours after infection with either E.coli or E.cloacae, compared to 90% survival for 18w^Δ7-35 heterozygotes, 18w^Δ1-12 homozygotes or wild-type third instar larvae. Homozygous 18w^Δ7-35 third instar larvae show 100% survival 24 hours after wounding with a sterile pyrogen-free needle.

(Williams et al., 1997)

Lethality is not complete. In uncrowded conditions 0.5% of the flies survive. Survivors eclose later than their sibs and die within a few days. Survivors typically show morphological defects in at least one appendage.

(Eldon et al., 1994)

External Data

Linkouts

Interactions

Show genetic interaction network for Enhancers & Suppressors

Phenotypic Class

Other

Statement

Reference

18w^k02701/18w^Δ7-35, Toll-6^ex13 has lethal phenotype

(Yagi et al., 2010)

18w^k02701/18w^Δ7-35, Df(3L)Brd15/Tollo⁵⁸ has lethal phenotype

(Yagi et al., 2010)

Phenotype Manifest In

Additional Comments

Genetic Interactions

Statement

Reference

18w^Δ7-35/18w^k02701; Toll-6^ex13/Toll-6^ex13 double mutants display 66% lethality.

18w^Δ7-35/18w^k02701; Tollo⁵⁸/Df(3L)Brd15 double mutants are lethal.

(Yagi et al., 2010)

In a hh^bar3/hh⁶ background 18w^Δ7-35 produces a dominant reduction in eye size resulting in a concave shape or nick at the anterior eye margin.

(Chiang and Beachy, 1994)

Xenogenetic Interactions

Statement

Reference

Complementation and Rescue Data

Comments

Images (0)

Mutant

Wild-type

Stocks (2)

Bloomington

4372

y¹ w^*; 18w^Δ7-35/CyO

Kyoto

107712

y¹ w^*; 18w^Δ7-35/CyO

Notes on Origin

Discoverer

Homozygous mutants develop up to the third larval instar, and after a prolonged duration of up to 4 days at this stage they die prior to pupation. Adult escapers are very occasionally observed. However, 18w^Δ7-35 in combination with Df(2R)017 (a deficiency which removes 18w) produces viable adults (although in numbers significantly lower than the expected Mendelian ratios) indicating that the lethality of homozygous 18w^Δ7-35 larvae is not entirely due to a lack of 18w product.

(Ligoxygakis et al., 2002)

External Crossreferences and Linkouts ( 0 )

Synonyms and Secondary IDs (4)

Reported As

Symbol Synonym

18w^7-35

(Mace et al., 2005, Ligoxygakis et al., 2002, Hedengren et al., 2000, Williams et al., 1997)

18w^Delta7-35

(Bivik et al., 2015, Christensen et al., 2008.9.3)

18w^Δ7-35

(Li et al., 2020, Nakamoto et al., 2012, Yagi et al., 2010)

Toll-2^Δ7-35

(Li et al., 2020)

Name Synonyms

Secondary FlyBase IDs

References (15)

Report Sections

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