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General Information
Symbol
Dmel\18wΔ7-35
Species
D. melanogaster
Name
FlyBase ID
FBal0033274
Feature type
allele
Associated gene
Associated Insertion(s)
Carried in Construct
Also Known As
18w7-35
Nature of the Allele
Mutations Mapped to the Genome
 
Type
Location
Additional Notes
References
Associated Sequence Data
DNA sequence
Protein sequence
 
 
Cytology
Nature of the lesion
Statement
Reference

Imprecise excision of the P{PZ} element present in 18w00053, resulting in a 2.2kb deletion extending from the site of the P{PZ} insertion into the 18w open reading frame.

Deletion of the P{PZ} element and 2.2kb of genomic DNA removing approximately 2.2kb of open reading frame.

Expression Data
Reporter Expression
Additional Information
Statement
Reference
 
Marker for
Reflects expression of
Reporter construct used in assay
Human Disease Associations
Disease Ontology (DO) Annotations
Models Based on Experimental Evidence ( 0 )
Disease
Evidence
References
Modifiers Based on Experimental Evidence ( 0 )
Disease
Interaction
References
Comments on Models/Modifiers Based on Experimental Evidence ( 0 )
 
Phenotypic Data
Phenotypic Class
Phenotype Manifest In
Detailed Description
Statement
Reference

18wΔ7-35/Df(2R)017 flies that have been challenged with vesicular stomatitis virus show levels of viral replication that are not significantly different to that seen in wild-type controls.

The 18wΔ7-35/Df(2R)017 combination results in viable adults (although in numbers significantly lower than the expected Mendelian ratios).

18wΔ7-35/Df(2R)017 flies show normal survival rates in response to infection with fungae or Gram-negative bacteria.

Only 56% of homozygous 18wΔ7-35 third instar larvae are still alive 24 hours after infection with either E.coli or E.cloacae, compared to 90% survival for 18wΔ7-35 heterozygotes, 18wΔ1-12 homozygotes or wild-type third instar larvae. Homozygous 18wΔ7-35 third instar larvae show 100% survival 24 hours after wounding with a sterile pyrogen-free needle.

Lethality is not complete. In uncrowded conditions 0.5% of the flies survive. Survivors eclose later than their sibs and die within a few days. Survivors typically show morphological defects in at least one appendage.

External Data
Interactions
Show genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
Additional Comments
Genetic Interactions
Statement
Reference

18wΔ7-35/18wk02701; Toll-6ex13/Toll-6ex13 double mutants display 66% lethality.

18wΔ7-35/18wk02701; Tollo58/Df(3L)Brd15 double mutants are lethal.

In a hhbar3/hh6 background 18wΔ7-35 produces a dominant reduction in eye size resulting in a concave shape or nick at the anterior eye margin.

Xenogenetic Interactions
Statement
Reference
Complementation and Rescue Data
Comments
Images (0)
Mutant
Wild-type
Stocks (2)
Notes on Origin
Discoverer

Homozygous mutants develop up to the third larval instar, and after a prolonged duration of up to 4 days at this stage they die prior to pupation. Adult escapers are very occasionally observed. However, 18wΔ7-35 in combination with Df(2R)017 (a deficiency which removes 18w) produces viable adults (although in numbers significantly lower than the expected Mendelian ratios) indicating that the lethality of homozygous 18wΔ7-35 larvae is not entirely due to a lack of 18w product.

External Crossreferences and Linkouts ( 0 )
Synonyms and Secondary IDs (4)
References (15)