Allele Dmel\Egfr^top-EC20

General Information
Symbol	Dmel\Egfr^top-EC20	Species	D. melanogaster
Name		FlyBase ID	FBal0033669
Feature type	allele	Associated gene	Dmel\Egfr
Also Known As	top^EC20, der^EC20

Map ( GBrowse )
Allele class	loss of function allele
Mutagen	ethyl methanesulfonate
Recent Updates
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FB2013_03
FB2013_02
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Nature of the Allele
Allele class	loss of function allele (Clifford and Schupbach, 1989)
Mutagen	ethyl methanesulfonate (Clifford and Schupbach, 1989, Clifford and Schupbach, 1994)
Mutations Mapped to the Genome

Type Location Additional Notes References point mutation 2R:17,443,460..17,443,460 evidence=experimental na_change=G17443460A pr_change=D382N\|Egfr-PA,D431N\|Egfr-PB reported_na_change=G1513A reported_pr_change=D382N (Clifford and Schupbach, 1994)
Associated Sequence Data
DDBJ / EMBL / GenBank	DNA sequence Protein sequence Name

UniProtKB/Swiss-Prot
UniProtKB/TrEMBL

Progenitor genotype
Nature of the lesion	Statement Reference Nucleotide substitution: G1513A. Amino acid replacement: D382N. (Clifford and Schupbach, 1994)
Cytology
Phenotypic Data
Phenotypic Class
	cell polarity defective (with Egfr^t1) (Gaengel and Mlodzik, 2003) lethal (Baker and Rubin, 1992) lethal \| pupal stage \| recessive (Clifford and Schupbach, 1994, Clifford and Schupbach, 1989)
Phenotype Manifest In
	ommatidium (with Egfr^t1) (Gaengel and Mlodzik, 2003)
Detailed Description
	Statement Reference Egfr^t1/Egfr^top-EC20 animals display rotation defects in adult ommatidia. (Gaengel and Mlodzik, 2003) Enhances the female sterility and adult morphological defects of Egfr^t1. Rarely survives as transheterozygote with the semi-viable Egfr^top-CA allele. (Clifford and Schupbach, 1994) Does not suppress the "Ellipse" phenotype. Hemizygotes die during pupariation, and have abnormally small dorsal imaginal discs. (Baker and Rubin, 1992) Homozygous embryos show no defects, hemizygotes are pupal lethal. When in combination with Df(2R)Egfr3 the eye-antennal, wing and haltere imaginal discs give rise to eyes, wings and halteres that are reduced size due to altered development of the imaginal disc. Embryos produced from heteroallelic combination with Egfr^t1 have a severe ventralised phenotype, reduction in size of their dorsal appendage. (Clifford and Schupbach, 1989)
External Data
Linkouts
Interactions
	Show the genetic interaction network for Enhancers & Suppressors
Phenotypic Class
Phenotype Manifest In
NOT Enhancer of
Statement Reference Egfr[+]/Egfr^top-EC20 is a non-enhancer of anterior crossvein phenotype of Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} (Callus and Mathey-Prevot, 2002)
Other
Statement Reference Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Egfr[+]/Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein L4 phenotype (Callus and Mathey-Prevot, 2002) Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein \| ectopic phenotype (Callus and Mathey-Prevot, 2002) Egfr^top-EC20, Scer\GAL4^en-e16E, Socs36E^{Scer\UAS.P\T.cCa} has wing vein L4 phenotype (Callus and Mathey-Prevot, 2002)
Additional Comments
Genetic Interactions
Statement Reference Expression of Socs36E^{Scer\UAS.P\T.cCa} under the control of Scer\GAL4^en-e16E in a Egfr^top-EC20/+ background results in ectopic wing vein material in 86% of flies and occasional thinning of a section of the L4 longitudinal wing vein. The anterior crossvein loss phenotype of Socs36E^{Scer\UAS.P\T.cCa}-expressing flies is not affected. (Callus and Mathey-Prevot, 2002)
Xenogenetic Interactions
Statement Reference
Complementation & Rescue Data
Comments
Stocks ( 0 )

Notes on Origin
Discoverer

Comments
	Class I allele. (Clifford and Schupbach, 1994) Mutation of Egfr that coordinately affects all gene activities, a class I lesion. The allelic series for class I lesions: Egfr^t2 = Egfr^t1 < Egfr^top-EC20 < Egfr^f7 = Egfr^f1 = Egfr^flb-2E07 < Egfr^top-EE39 = Egfr^top-ED26 = Egfr^f5 < Egfr^f9 = Egfr^f10 = Egfr^f2 = Egfr^top-EE42 = Egfr^f11 = Egfr^f24 = Egfr^f3 = Df(2R)Egfr3. (Clifford and Schupbach, 1989)
External Crossreferences & Linkouts
Other Crossreferences

Linkouts

Synonyms & Secondary IDs ( 5 )
Reported As
Symbol Synonym	der^EC20 (Callus and Mathey-Prevot, 2002) DER^EC20 (Schnepp et al., 1996) Egfr^EC20 (Gaengel and Mlodzik, 2003) Egfr^top-EC20 top^EC20 (Clifford and Schupbach, 1994, Baker and Rubin, 1992, Clifford and Schupbach, 1989)
Name Synonym
Secondary FlyBase IDs

References ( 6 )
Research paper	Gaengel and Mlodzik, 2003, Development 130(22): 5413--5423 Egfr signaling regulates ommatidial rotation and cell motility in the Drosophila eye via MAPK/Pnt signaling and the Ras effector Canoe/AF6. [FBrf0167499] Callus and Mathey-Prevot, 2002, Oncogene 21(31): 4812--4821 SOCS36E, a novel Drosophila SOCS protein, suppresses JAK/STAT and EGF-R signalling in the imaginal wing disc. [FBrf0151456] Schnepp et al., 1996, Genes Dev. 10(18): 2302--2313 Vein is a novel component in the Drosophila epidermal growth factor receptor pathway with similarity to the neuregulins. [FBrf0090788] Clifford and Schupbach, 1994, Genetics 137(2): 531--550 Molecular analysis of the Drosophila EGF receptor homolog reveals that several genetically defined classes of alleles cluster in subdomains of the receptor protein. [FBrf0072848] Baker and Rubin, 1992, Dev. Biol. 150: 381--396 Ellipse mutations in the Drosophila homologue of the EGF receptor affect pattern formation, cell division, and cell death in eye imaginal discs. [FBrf0055813] Clifford and Schupbach, 1989, Genetics 123: 771--787 Coordinately and differentially mutable activities of torpedo, the Drosophila melanogaster homolog of the vertebrate EGF receptor gene. [FBrf0049928]

Allele Dmel\Egfrtop-EC20

Allele Dmel\Egfr^top-EC20